A direct current glow discharge plasma was generated on a canonical 2.75° half-angle wedge test article. Experiments were conducted in the Texas A&M University Actively Controlled Expansion Tunnel at M=5.7 and Re=6×106/m. The effects of Reynolds number, polarity, and upstream perturbation were all independently studied via imaging, power measurements, and optical emission spectroscopy (OES). These measurements are the first to be conducted in a tripped hypersonic boundary layer and allow a deeper understanding of the interplay between the plasma and tripped flow. The plasma, formally characterized as a normal glow discharge, had negligible Joule and cathode heating effects on the boundary layer. Analogously, the installation of trips upstream of the electrodes changed only the appearance of the plasma, creating periodic streaks corresponding to the wakes and vortices produced by each trip but not altering the power across the electrodes. The OES indicates generation of nitric oxide (NO) in both the positive column and, mainly, at the cathode at the relatively low plasma power of ∼47W.