Dentine hypersensitivity (DH) is a common condition that is frequently encountered in dental practice. The aim of this multi-centre and cross-sectional study was to establish the prevalence of DH in the general population in China. A total of 6843 subjects were questioned and examined using a triple syringe with a blast of cold air to confirm the diagnosis of DH. The sensitive surface, loss of attachment and gingival recession of sensitive teeth were measured and recorded. Of the 2932 subjects reporting hypersensitive symptoms, 2363 subjects were further diagnosed as DH, giving an overall prevalence of 34·5%. The greatest number of subjects with DH were within the 50-59 years age group (P < 0·01). The most commonly affected teeth were the premolars and the most provoking factor was the presence of a cold stimulus. The prevalence of DH in a representative sample of the general population in China was 34·5%, indicating that DH is a common oral problem. Therefore, the recognition of the condition and subsequent treatment by the dentist is therefore essential if the condition is to be managed correctly.