Trinity University Research Articles

Testing Interdisciplinarity in the Field

The Interview connects Bulletin readers with scholars who have been shaping key aspects of our work in the field. Suzanne Owen at Leeds Trinity University in the UK takes some time to chat with us. Owen will be talking about her experiences in both U.S. and U.K. education systems, her path to the academy through Edinburgh courses, Canadian fieldwork, and the BASR. She and Newton also touch on the issues she sees in the UK Academy and where she hopes Religious Studies can go from here. We thank her for her time and willingness to be part of this issue of the Bulletin.

<i>Critics Not Caretakers</i>...The Public Study of Religion 20+ Years In

The topic of our conversation is Russell T. McCutcheon’s Critics Not Caretakers: Redescribing the Public Study of Religion (SUNY Press 2001). This provocative volume continues to be discussed and argued within the field, warranting an updated volume. With the release of its expanded second edition (Routledge 2024) release, the Bulletin’s editorial team thought readers would appreciate a broader conversation about the book’s impact, legacy, and potential future in framing the academic study of religion. We are joined by Leslie Smith (Avila University), Mitsutoshi Horii (Chaucer College, UK and Shumei University), Suzanne Owen (Leeds Trinity University), and Titus Hjelm (University of Helsinki) as our discussants.

Evaluating Physiological and Organizational Properties of the Tight Junction ZO-1 ZU-5 Domain in MDCK Cells

Zonula occludens-1 (ZO-1) is an essential TJ component contributing to junctional formation by mediating interactions between junctional proteins and the actomyosin cytoskeleton. MDCK II cells, a model epithelial cell line, with ZO-1 knocked-out (ZO-1 KO) have observable phenotypic changes such as alterations in the actin cytoskeletal organization and physiological barrier properties. This study focuses on the ZO-1-ZU5 domain by rescuing ZO-1 with full-length and a panel of ZO-1-ZU5 mutants. ZO-1-ZU-5 is known to bind cingulin and through this interaction ZO-1 adopts an extended conformation. We examined actomyosin cytoskeleton organization, ZO-1 mobility, gene expression and barrier function to determine whether the ZO-1-ZU5 mutants rescue full-length activity. We found that the ZO-1- ZU5 truncation mutant cell line rescued transepithelial electrical resistance (TER) but did not rescue tight junction distribution or the amount of total gamma-actin. We will present evaluation of additional ZU5 mutants. This study expands our understanding of how extended ZO-1 regulates the mechanical, genetic, and phenotypic properties of epithelial cells. Trinity University Biology Summer Undergraduate Research Fellowship. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Marketing Higher Education in Texas: Trinity University as a Case Study

Marketing Higher Education in Texas: Trinity University as a Case Study

Microcell concentrating photovoltaics for space

Christian Ruud received his PhD in materials science and engineering at Penn State University under the guidance of Dr. Chris Giebink. His doctoral research was funded by a NASA Space Technology Graduate Research Opportunities award and covered microcell concentrating photovoltaics for space and graded-index antireflection coatings. He also holds a bachelor’s degree in chemistry from St. Olaf College in Northfield, Minnesota. Recently, Christian began a new role as a research engineer at PPG in Pittsburgh, Pennsylvania. Jeffrey Gordon is professor emeritus at Ben-Gurion University of the Negev's Department of Solar Energy and Environmental Physics in Israel, where he has been a faculty member for 45 years. He played a pivotal role in stewarding it to an internationally recognized center of research and graduate education. His research advances have led to over 250 publications in leading scientific journals and cover the fields of nanomaterial synthesis, solar energy conversion, advanced optical design, finite-time thermodynamics, biomedical science, and algae biotechnology, with several innovations having been translated into impactful commercial realities. Chris Giebink has been a professor of electrical engineering at Penn State University for 10 years and is presently moving to the Department of Electrical Engineering and Computer Science at the University of Michigan. His research on optoelectronic and photonic devices has led to more than 80 publications and 13 patents. He received his PhD in electrical engineering from Princeton University and holds undergraduate degrees in physics and engineering science from Trinity University. Christian Ruud received his PhD in materials science and engineering at Penn State University under the guidance of Dr. Chris Giebink. His doctoral research was funded by a NASA Space Technology Graduate Research Opportunities award and covered microcell concentrating photovoltaics for space and graded-index antireflection coatings. He also holds a bachelor’s degree in chemistry from St. Olaf College in Northfield, Minnesota. Recently, Christian began a new role as a research engineer at PPG in Pittsburgh, Pennsylvania. Jeffrey Gordon is professor emeritus at Ben-Gurion University of the Negev's Department of Solar Energy and Environmental Physics in Israel, where he has been a faculty member for 45 years. He played a pivotal role in stewarding it to an internationally recognized center of research and graduate education. His research advances have led to over 250 publications in leading scientific journals and cover the fields of nanomaterial synthesis, solar energy conversion, advanced optical design, finite-time thermodynamics, biomedical science, and algae biotechnology, with several innovations having been translated into impactful commercial realities. Chris Giebink has been a professor of electrical engineering at Penn State University for 10 years and is presently moving to the Department of Electrical Engineering and Computer Science at the University of Michigan. His research on optoelectronic and photonic devices has led to more than 80 publications and 13 patents. He received his PhD in electrical engineering from Princeton University and holds undergraduate degrees in physics and engineering science from Trinity University.

Understanding the role of ZO-1 in cell phenotype and gene expression through myosin inhibition

Zonula occludens-1 (ZO-1) is a tight junction protein contributing to junctional formation as well as mediating interactions with junctional proteins and the actomyosin cytoskeleton. To investigate the role of ZO-1, Madin-Darby canine kidney (MDCK) II knockout cells lacking ZO-1 were created utilizing a CRISPR gene editing platform. These ZO-1 KO cells display characteristic phenotypic changes including actin accumulation and junction linearization. We evaluated membrane tension and found ZO-1 loss leads to increased membrane tension and nuclear density. We hypothesized that due changes in cytoskeletal organization and membrane tension the ZO-1 KO cells would spread less following plating. It is suspected that phenotypic changes related to actin accumulation may be the result of myosin dysregulation in the absence of ZO-1. ZO-1 KO cells that are exposed to blebbistatin, a myosin II inhibitor, resemble wild type cells in cell height and junction linearization. RNAseq and qRT-PCR were used to investigate gene expression changes related to ZO-1 loss to further interrogate ZO-1’s role in regulating membrane tension. This study highlights and expands our understanding of how ZO-1 regulates the mechanical properties of epithelial cells. Trinity University, Mach Research Fellowship This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Inducing neuronal characteristics of HT22 cells through differentiation with pro-neural factors

We are interested in using an easily cultured immortalized neuronal cell line as a replacement for primary cultures. HT22 cells are an SV40 transformed, immortalized mouse hippocampal neuron cell line, that have been used under the assumption that they will function similar to primary culture neurons. However, this assumption may be inaccurate since HT22 cells show low expression of plasmids containing neuron specific promoters. We hypothesized that differentiation of HT22 cells with removal of sera and addition of pro neural factors, would improve their neuronal characteristics. We characterize the neuronal qualities of HT22 cells grown with and without sera and after switching to a neuron supporting media containing either N2 or B27 supplements. These supplements are consistently used in the culturing of primary neurons and the differentiation of immortalized neurons. Additionally, the effects of nerve growth factor (NGF) and retinoic acid (RA) to further induce and support neuronal characteristics is being tested. NGF has been shown to induce differentiation and structural changes in HT22 cells into neuronal cells, and RA has been shown to regulate a late stage neuronal differentiation promoter, CaMKIIα. HT22 cell differentiation is characterized by multiple metrics. Cell cycle progression analyses are performed using propidium iodide (PI) staining and measured using flow cytometry, as differentiated HT22 cells are mitotically arrested and would be prevented from entering S phase. Cell death assays are also performed, using a combination of PI and Annexin V staining to determine if post differentiation HT22 cells die by apoptosis, unlike pre-differentiation HT22 cells. Post differentiation, HT22 cell CaMKIIα expression levels are determined using green fluorescent protein (GFP) as a reporter gene and measured by confocal imaging. We have also characterized the membrane properties and expression of ion channels post-differentiation using electrophysiology. Results indicate differentiation with a combination of N2 supplement, RA, and NGF has the greatest improvement of HT22 cell neuronal characteristics compared to undifferentiated HT22 cells. An increase in apoptosis rates has been observed in HT22 cells differentiated with B27 supplement alone, but rates of apoptosis seem to decrease when differentiated with B27 supplement, NGF, and RA. Using this model, we hope to characterize gene expression from viruses and plasmids containing neuronal promoters. Funding Sources: Murchison Summer Research Fellowship, McNair Scholars Program, Biology Summer Research Fellowship, Trinity University Start-Up Funds, The Brain and Behavior Research Foundation NARSAD Young Investigator Award, and the Mary E. Groff Foundation This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Using synaptic connections to dissect neural microcircuitry

There are a number of methods used to study synapses through selective labeling and control. However, these techniques are often cytotoxic, difficult to implement, or lack the selectivity required to most effectively study microcircuits. We aim to create two novel techniques for studying how the brain is wired – a method for transsynaptic genetic labeling and a method for selectively destroying synapses. For synaptic labeling we are using the C. elegans orthologs for the Notch-Delta signaling system, Glp-1 and Lag-2. Glp-1 was modified by replacing the intracellular portion of the protein with cre recombinase, a site directed DNA recombination enzyme. It is hypothesized that when a cell expressing the chimeric Glp1-Cre protein comes in close contact with a cell expressing Lag-2, cre recombinase will be released allowing for the selective expression of a fluorescent molecule based on cell proximity. In vitro testing via confocal microscopy indicates that the labeling mechanism performs as expected, labeling cells based on contact in HEK 293T cells.To selectively destroy synapses, we have developed a chimeric protein, CadPlexin, that will activate repulsion signals through homophilic binding between two cells connected by a synapse. CadPlexin is composed of the extracellular domain of fruit fly DE-Cadherin and the transmembrane and intracellular domains of mouse plexin-B2. Cadherins bind to one another homophillically, acting to bind cells with one another. Plexins, when dimerized and activated by their usual ligand, Semaphorin, will cause a downstream signaling cascade that induces cell-cell repulsion. We hypothesize that, when two cells expressing CadPlexin are in close contact (i.e. at a synapse), CadPlexin will selectively destroy the synapse, as the extracellular domains from DE-Cadherin will bind together and mimic semaphorin binding and clustering required to activate plexin signaling. Preliminary results from in vitro experiments suggest that CadPlexin performs as expected, seen through stress fibers in the actin cytoskeleton of Cos7 cells transfected with CadPlexin – a characteristic cytoskeletal change induced through Plexin signaling. Together, these techniques will allow us to label synaptically connected neurons and destroy synapses with surgical precision. Funding Sources: Murchison Summer Research Fellowship, Trinity University Start-Up Funds, McNair Scholars Program, the Brain and Behavior Research Foundation NARSAD Young Investigator Award, Mary E. Groff Foundation, Women in STEM Program. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Hemisphere-specific characterization of projections from the Pedunculopontine Tegmental Nucleus to Substantia Nigra pars Compacta dopaminergic neurons

The neurotransmitter dopamine has been shown to be implicated in motivation and reward, notably in the nigrostriatal dopaminergic pathway. Within this pathway, our research focuses on the synapse between the midbrain regions pedunculopontine tegmental nucleus (PPN) and substantia nigra pars compacta (SNc). The PPN is unusual in that it sends projections to both ipsilateral and contralateral sides of the brain, unlike other brain structures that confine their innervations to either ipsilateral or contralateral. To understand the nature of these inputs, the current study focuses specifically on the glutamatergic excitatory projections as well as the GABAergic inhibitory projections within the PPN-SNc circuit using optogenetics in combination with electrophysiological and confocal microscopic methods. These projections have not been previously studied due to a lack of tools. The goal of this particular study is to investigate how hemisphere-specific inputs differ in their synaptic strength. Stereotaxic surgery is performed to unilaterally deliver virus that encodes expression for a depolarizing light-gated ion channel, Channelrhodopsin (ChR2), tagged with yellow fluorescent protein. Light-gated channels were activated to selectively depolarize PPN axons located in the input region that subsequently stimulates synapses onto dopamine neurons. Electrophysiological tests were then run to obtain electrical recordings that permit the quantification of excitatory and inhibitory responses within the PPN-SNc pathway. Finally, brain slices were immunostained with antibodies to tyrosine hydroxylase, a precursor to dopamine, glutamic acid decarboxylase, a GABA-synthesizing enzyme, and vesicular glutamate transporter to visualize dopaminergic neurons, GABAergic projections, and glutamatergic projections, respectively. Confocal images were collected in order to quantify the macroscopic distribution of ipsilateral and contralateral projections in the SNc. We are also using injections of retrograde beads into the SNc to label projecting neurons in the PPN to confirm the hemisphere specificity seen in anterograde labeling.Preliminary findings suggest GABAergic PPN efferents project only to ipsilateral SNc. However, glutamatergic PPN efferent project to both ipsilateral and contralateral SNc, with differing strengths. Our research will permit greater clarity for future studies which seek to understand the underlying neural network and synaptic-level processes of the nigrostriatal dopaminergic pathway. Acknowledgements: This research was supported by: Murchison Summer Research Fellowship, Biology Summer Research Fellowship, Trinity University Start-Up Funds, McNair Scholars Program, the Brain and Behavior Research Foundation NARSAD Young Investigator Award, and the Mary E. Groff Foundation. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Contributors to this Issue

Other| March 01 2023 Contributors to this Issue Nineteenth-Century Literature (2023) 77 (4): 268–269. https://doi.org/10.1525/ncl.2023.77.4.268 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Contributors to this Issue. Nineteenth-Century Literature 1 March 2023; 77 (4): 268–269. doi: https://doi.org/10.1525/ncl.2023.77.4.268 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentNineteenth-Century Literature Search Gerard Lee McKeever is Lecturer in Scottish Literature in the Department of English and Scottish Literature at the University of Edinburgh. He is the author of Dialectics of Improvement: Scottish Romanticism, 1786–1831 (2020), winner of the BARS First Book Prize 2021. He is currently finishing a monograph titled Regional Romanticism: Southwest Scotland, c.1770–1830 for Palgrave Macmillan, as well as editing John Galt’s autobiographies for Angela Esterhammer’s “Edinburgh Edition of the Works of John Galt” series. Claudia Stokes is Professor of English at Trinity University. She is the author of Writers in Retrospect: The Rise of American Literary History, 1875–1910 (2006); The Altar at Home: Sentimental Literature and Nineteenth-Century American Religion (2014); and Old Style: Unoriginality and Its Uses in Nineteenth-Century U.S. Literature (2022). She is currently preparing a scholarly edition of Harriet Beecher Stowe’s religious writings for Oxford University Press. * * *... You do not currently have access to this content.

Editorial

This special issue of the Interactive Film and Media Journal constitutes the first of two issues dedicated to the IV International Interactive Film and Media Conference, held online in June 2022 and co-organized by Hudson Moura (Toronto Metropolitan University, Canada), Heidi Rae Cooley (University of Texas at Dallas, USA), Anna Wiehl (University of Bayreuth, Germany) and Stefano Odorico (Technological University of the Shannon, Ireland / Leeds Trinity University, UK). As tangential as it may seem, the IFM conferences are an opportunity to reflect on the scholarly field of interactivity, offering a platform for academics and practitioners to broaden the field across disciplines. The conference titled: Interactive Epistemology, Listening, and Ecomedia clearly demonstrated that interactivity does not just have a newly rediscovered popularity in film and media, but it is also omnipresent in a multidisciplinary context, from cultural productions such as games, films and books to audience perception such as streaming, virtual reality and real-time; from intellectual publications such as news, hypertexts, social media to virtual world economy such as cryptocurrencies.

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The Trinity University,2022/2023 pre-degree/ jupeb Admission Form Is Out ☎️+23470-6508-6538 ☎️ 07065086538 Prof Mrs Faith candidates are hereby enjoined to purchase the form and apply for suitable Undergraduate Courses Candidates must have at least 6 (six) "Cs (Credits)" grades in their O'level result (s)Candidates must score at least 120 in JAMB UTME examination.This must include English Language, Mathematics and three

The Trinity University,2022/2023 pre-degree/ jupeb Admission Form Is Out ☎️+23470-6508-6538 ☎️ 07065086538 Prof Mrs Faith candidates are hereby enjoined to purchase the form and apply for suitable Undergraduate Courses Candidates must have at least 6 (six) "Cs (Credits)" grades in their O'level result (s)Candidates must score at least 120 in JAMB UTME examination.This must include English Language, Mathematics and three

Predegree Form for Trinity University Ogun 2022/2023 Admission/ Direct Entry (DE) Form Call 0913-423-4770 Dr Mrs.Anita for more details on Remedial/Transfer form,Remedial/Direct Entry Form,PhD/PgD Form call (0913-423-4770 Mrs Judith (0913)4234770,.This is to inform the general public that the PreDegree Application form 2022/2023 session is out on sale call (0913)423-4770 mrs anita (0913)4234770 now.

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Other| July 01 2022 Information about Contributors Journal of American Folklore (2022) 135 (537): 379–380. https://doi.org/10.5406/15351882.135.537.27 Cite Icon Cite Share Icon Share Facebook Twitter LinkedIn MailTo Permissions Search Site Citation Information about Contributors. Journal of American Folklore 1 January 2022; 135 (537): 379–380. doi: https://doi.org/10.5406/15351882.135.537.27 Download citation file: Zotero Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All Scholarly Publishing CollectiveUniversity of Illinois PressJournal of American Folklore Search Advanced Search Norma E. Cantú, Norine R. and T. Frank Murchison Distinguished Professor of the Humanities at Trinity University in San Antonio, Texas, served as the President of the American Folklore Society (2019–2021). She is Professor Emerita in English at the University of Texas at San Antonio. Founder and director of the Society for the Study of Gloria Anzaldúa, she organized El Mundo Zurdo, a gathering of Anzalduístas from 2007–2019. She is a folklorist, scholar, poet, and novelist whose scholarly and creative focus is on the United States-Mexico borderlands; her most recent publications include the novel Cabañuelas (2019); Meditación Fronteriza: Poems of Love, Life, and Labor (2019), and the coedited anthologies Entre Guadalupe y Malinche: Tejanas in Literature and Art (with Inés Hernández-Ávila, 2016), meXicana Fashions: Politics, Self-Adornment, and Identity Construction (with Aída Hurtado, 2020), Teaching Gloria Anzaldúa: Pedagogy and Practice for Our Classrooms and Communities (with Margaret Cantú-Sánchez and Candace de... You do not currently have access to this content.

Information about Contributors

Other| April 01 2022 Information about Contributors Journal of American Folklore (2022) 135 (536): 262–264. https://doi.org/10.5406/15351882.135.536.19 Cite Icon Cite Share Icon Share Twitter Permissions Search Site Citation Information about Contributors. Journal of American Folklore 1 January 2022; 135 (536): 262–264. doi: https://doi.org/10.5406/15351882.135.536.19 Download citation file: Zotero Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All Scholarly Publishing CollectiveUniversity of Illinois PressJournal of American Folklore Search Advanced Search Maribel Alvarez is the Jim Griffith Chair in Public Folklore at the Southwest Center, University of Arizona, where she is also Associate Dean for Community Engagement in the College of Social & Behavioral Sciences. She is the founder and until recently served as executive director of the Southwest Folklife Alliance, an independent nonprofit affiliated with the University of Arizona. She has served on the faculty of the National Association of Latino Arts and Cultures’ National Leadership Institute for 16 years. In 2018, the American Folklore Society awarded her the prestigious Américo Paredes Prize for “excellence in integrating scholarship and engagement with the people and communities one studies.”Norma E. Cantú, the Norine R. and T. Frank Murchison Distinguished Professor of the Humanities at Trinity University in San Antonio, Texas, served as the president of the American Folklore Society from 2019 through 2021. She is Professor Emerita in English at... You do not currently have access to this content.