Triester Approach Research Articles

Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides1

The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.

Use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach

Use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach

Improved triester approach for the synthesis of pentadecathymidylic acid.

Improved triester approach for the synthesis of pentadecathymidylic acid.