In this paper we prove that under some conditions on the parameters the univariate Triebel–Lizorkin spaces Fr,qs(R) can be characterized in terms of quarklets. So for functions from Triebel–Lizorkin spaces we obtain a quarkonial decomposition as well as a new equivalent quasi-norm. For that purpose we use quarklets that are constructed by means of biorthogonal compactly supported Cohen–Daubechies–Feauveau spline wavelets, where the primal generator is a cardinal B-spline. Moreover we introduce some sequence spaces apposite to our quarklet system and study their properties. Finally we also obtain a quarklet characterization for the Triebel–Lizorkin–Morrey spaces Eu,r,qs(R).