Levels Of Triclocarban Research Articles

Determination of triclocarban and triclosan in urine by QuEChERS extraction and ultra-performance liquid chromatography-tandem mass spectrometry

Objective: To establish a method for the determination of triclocarban (TCC) and triclosan (TCS) in urine by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) after purification by QuEChERS. Methods: In May 2022, urine samples were extracted by acetonitrile, purified by QuEChERS, separated by Waters Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm), and eluated with water-acetonitrile as mobile phase gradient at a flow rate of 0.3 ml/min. The detection was conducted in negative ion mode (ESI(-)) and multiple reaction monitoring (MRM) scanning, it was quantified with a internal standard method, and the methodology was verified. Results: The linear ranges of TCC and TCS were 0.5-100.0 μg/L and 1.0-100.0 μg/L, and the correlation coefficients were 0.9997 and 0.9991, respectively. The limits of detection and quantitation of TCC and TCS were 0.17 and 0.33 μg/L, and 0.5 and 1.0 μg/L, respectively. The recoveries of TCC and TCS were 100.1%-102.8% and 96.7%-108.6%, and the relative standard deviations were 4.9%-6.7% and 4.1%-8.3%, respectively, at 2.0, 10.0 and 80.0 μg/L. Conclusion: QuEChERS-UPLC-MS/MS method is simple, rapid, sensitive and reproducible, and can be used for rapid and accurate simultaneous detection of TCC and TCS exposure levels in occupational population.

Trimester-specific exposure to triclocarban during pregnancy: Associations with oxidative stress and size at birth

Triclocarban (TCC) is an extensively used antimicrobial agent that exhibits endocrine disrupt potential, but its effects on fetal growth remain largely unknown. Herein, we measured TCC, its four hydroxylated metabolites and two dechlorination products, as well as the oxidative stress biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) in maternal urine samples collected across three trimesters of pregnancy in Wuhan, China. Linear mixed-effect models and multiple linear regression models were applied for correlation analysis. TCC was detected in >97 % of urine samples after conjugate hydrolysis (geometric mean: 0.249–0.335 ng/mL). An interquartile range increase in TCC was associated with a 6.65 % increase in 8-OHdG (95 % confidence interval: 2.15–11.16 %). Urinary TCC in the first trimester was inversely associated with body weight in infant girls, with significant p-value for trend (ptrend = 0.011) across tertiles of TCC concentrations. Urinary 8-OHdG in the third trimester was associated with reduced ponderal index in infant boys (ptrend = 0.020). Urinary levels of TCC correlated well with its transformation products (2′-OH-TCC, 3′-OH-TCC, 6-OH-TCC, 4′-DHC, and DCC). No clear association was found between these metabolites and 8-OHdG, as well as size at birth. Our results revealed the potential exposure risks of TCC during the early life stage, future replications in other populations are needed.

Association of urinary triclosan, methyl triclosan, triclocarban, and 2,4-dichlorophenol levels with anthropometric and demographic parameters in children and adolescents in 2020 (case study: Kerman, Iran).

Endocrine-disrupting chemicals (EDCs) can be a major risk factor for noncommunicable illnesses, especially when children are exposed to them. The purpose of this study was to assess the urine concentrations of triclosan (TCS), methyl triclosan (MTCS), triclocarban (TCC), and 2,4-dichlorophenol (2,4-DCP) and its association with anthropometric and demographic parameters in children and adolescents aged 6–18 living in Kerman, Iran, in 2020. A GC/MS instrument was used to measure the concentrations of the analytes. TCS, MTCS, TCC, and 2,4-DCP geometric mean concentrations (µg/L) were 4.32 ± 2.08, 1.73 ± 0.88, 4.66 ± 10.25, and 0.19 ± 0.14, respectively. TCS, MTCS, TCC, and 2,4-DCP were shown to have a positive and significant association with BMI z-score and BMI (p-value < 0.01). TCS and MTCS have a positive, strong, and substantial association (p-value < 0.01, r = 0.74). There was no significant association between the waist circumference (WC) and the analytes studied. In addition, there was a close association between analyte concentration and demographic parameters (smoking, education, income, etc.) overall. In Kerman, Iran, the current study was the first to look into the association between TCS, MTCS, TCC, and 2,4-DCP analytes and anthropometric and demographic data. The levels of urinary TCS, MTCS, TCC, 2,4-DCP, and anthropometric parameters in children and adolescents are shown to have a significant association in this study. However, because the current study is cross-sectional and it is uncertain if a single experiment accurately reflects long-term exposure to these analytes, more research is needed to determine the impact of these analyses on the health of children and adolescents.

The fate of triclocarban in activated sludge and its influence on biological wastewater treatment system

Triclocarban (TCC), a typical emerging contaminant, was abundantly released into environment and frequently detected in practical wastewater treatment plants. However it is also an important material when being added to personal skin care products as a antibacterial agent. In this work, the behavior of TCC in wastewater treatment process was investigated. Experiments showed that ~82% of influent TCC was removed by activated sludge adsorption and its adsorption isotherm was well fitted with Linear model and Freundich model. High levels of TCC had seriously impact on the settleability, dewaterability and extracellular polymetric substance (EPS) of activated sludge, even on effluent turbidity after a long-term exposure. Furthermore, the performance of biological wastewater treatment was damaged by TCC long-term exposure as well. The removal rates of total nitrogen and phosphorus decreased from 91.2 ± 2.1% to 72.6 ± 2.2% and from 94.7 ± 3.1% to 78.4 ± 2.3%, respectively, with TCC level increasing from 0 to 100 μg/L. Mechanism analysis showed that TCC exposure significantly inhibited the relevant biological processes, such as ammonia oxidation, denitrification, phosphorus release and uptake, which were closely relevant to nitrogen and phosphorus removal.

Occurrence and fate of antimicrobial triclocarban and its transformation products in municipal sludge during advanced anaerobic digestion using microwave pretreatment

This study, for the first time, investigated the impact of microwave pretreatment on the fate of the pervasive antimicrobial triclocarban (TCC) that was already present in municipal sludge, before and during advanced anaerobic digestion (AD) under thermophilic and mesophilic conditions. A range of microwave temperature (80 and 160 °C) and exposure duration (1 and 30 min) configurations were studied by employing ten bench-scale anaerobic digesters fed with mixed sludge at three different solids retention times (SRTs) including 20, 12, and 6 days. Seasonal changes influenced the levels of TCC in municipal sludge sampled from a plant employing the biological nutrient removal. Initial batch pretreatment studies showed that microwave irradiation itself can achieve TCC removal efficiencies up to 30 ± 4 and 64 ± 5% at 80 and 160 °C, respectively. The control digesters utilizing un-pretreated mixed sludge showed limited TCC removals, between 18 and 32% and 11–26% respectively, under thermophilic and mesophilic temperatures. On the other hand, the highest TCC elimination (78 ± 2%) was obtained from the thermophilic digester utilizing microwaved sludge at 160 °C for 30 min at SRT of 12 days. The non-chlorinated carbanilide (a transformation product of TCC) was detected and quantified for the first time during conventional and microwave-pretreated anaerobic sludge digestion. The formation of carbanilide in biosolids through reductive dechlorination could be an indicator of efficient and complete TCC transformation. This research demonstrated that AD coupled with microwave pretreatment can be used to reduce environmental concentrations of TCC in municipal sludge and biosolids.

Fate of Triclocarban (TCC) in aquatic and terrestrial systems and human exposure

Triclocarban (TCC) is considered as contaminant of emerging concern (CEC), and ranked in the top 10 CEC occurrence. TCC is a high production volume synthetic chemical used extensively in various personal care products. This chemical will be released into the environment via incomplete wastewater treatment and untreated wastewater discharge. TCC and its transformation products (4,4′-dichlorocarbilide (DCC),1-(3-chlorophenyl)-3-phenylurea (MCC) and carbanilide (NCC),2′OH-TCC, 3′OH-TCC) were detected in the environmental matrices. Sediment organic carbon will influence TCC concentrations in suspended and bed sediments. TCC is an antimicrobial agent and also emerging endocrine disruptor that can cause immune dysfunction and affect human reproductive outcomes. Furthermore, TCC alters the expression of proteins related to binding and metabolism, skeletal muscle development and function, nervous system development and immune response. TCC has potential health risks in wildlife and humans. Several animal studies illustrate that it can cause various adverse effects, which can be monitored by antioxidant biomarkers (CAT, GST and LPO). Accumulation of TCC in organisms depends on the lipophilicity and bioavailability of TCC in sediment and water. TCC was continuously detected in aquatic system. TCC is a lipophilic compound, which can efficiently bind with lipid content. Women are more vulnerable to TCC due to substantially higher frequency and extended exposure to TCC. This review provides basic information of occurrence of TCC and the exposure levels in aquatic organisms. Several literature have shown the higher usage and human exposure levels of TCC, which provides useful information for the chemical management approaches.

Assessment of human exposure to triclocarban, triclosan and five parabens in U.S. indoor dust using dispersive solid phase extraction followed by liquid chromatography tandem mass spectrometry.

Antimicrobials in indoor dust pose concerns due to their endocrine disrupting activities and potential promotion of antibiotic resistance. We adopted dispersive solid phase extraction (d-SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify antimicrobials in dust. The method showed favorable linearity (R2 >0.99), recovery (83-115%), and method detection limits (1.2-5.6 ng/g, dry weight). All seven analytes were found at median concentrations in ng/g in each of the 80 U.S. dust samples collected from athletic facilities and residential homes: methyl paraben (1920) > propyl paraben (965) > triclosan (390) > triclocarban (270) > ethyl paraben (195) > butyl paraben (80) > benzyl paraben (6). Triclosan levels in dust from athletic facilities were significantly higher than those in private homes (p < 0.05). Median estimated daily intake (EDI) of antimicrobials in ng/kg-body weight/d from dust ingestion was lowest for adults (1.9) and higher for more sensitive subpopulations, including infants (19.8), toddlers (23.6), children (11.8) and teenagers (4.6). This first application of d-SPE to the analysis of dust produced U.S. baseline data for triclosan and triclocarban levels in indoor dust just prior to the 2017 Federal ban on use of these trichlorinated aromatics in antiseptic soaps and related personal care products.

Urinary levels of triclosan and triclocarban in several Asian countries, Greece and the USA: Association with oxidative stress

Triclosan (TCS) and Triclocarban (TCC) are widely used as antimicrobial preservatives in personal care products (PCPs). Because of their potential for endocrine disrupting effects, human exposure to these chemicals is a concern. Biomonitoring studies of human exposure to TCS and TCC have shown widespread exposure of populations in western European countries and the USA. However, exposure to TCC and TCS by populations in Asian countries is less well known. In this study, concentrations of TCS and TCC were determined in human urine collected from seven Asian countries (China, India, Korea, Kuwait, Japan, Saudi Arabia, and Vietnam), and Greece and the USA. A total of 430 urine samples were analyzed for TCS and TCC, of which 355 (83%) and 82 (19%), respectively, contained measurable levels of these chemicals. The overall geometric mean [GM] concentrations of TCS and TCC, were 1.36 and 0.03ng/mL, respectively. The highest mean concentration of TCS was found in urine from China (100ng/mL) and the lowest concentration was found in urine from Vietnam (2.34ng/mL). We also analyzed urinary 8-OHdG, a marker of oxidative stress, to elucidate the association with TCS and TCC levels for samples from Saudi Arabia (n=130) and a positive correlation between Ln-transformed TCC levels and 8-OHdG was found, although this was not statistically significant. This is the first study to report urinary levels of TCS and TCC in several Asian countries, especially for Vietnam, Kuwait, and Japan.

Triclosan/triclocarban levels in maternal and umbilical blood samples and their association with fetal malformation

Triclosan (TCS) and triclocarban (TCC) are widely used as antimicrobial compounds in consumer products. TCS and TCC are frequently found in waste water and sewage. In this study, we investigate the potential impact of exposure to triclosan (TCS) and triclocarban (TCC) on fetal abnormalities. We measured TCS and TCC levels in maternal and umbilical cord blood samples from 39 pregnant women diagnosed with fetal or post-birth abnormalities at Beijing Obstetrics and Gynecology Hospital. 52 pregnant women who gave birth to healthy neonates during the same period of time were included as controls. Applying ultra-performance liquid chromatography-tandem mass spectrometry, TCS and TCC concentrations were measured in maternal and fetal sera. Significantly increased levels of TCS were detected in maternal sera from mothers with abnormal births. Similar levels of TCS or TCC were found in maternal and cord sera in control group. The concentrations of TCS or TCC in maternal sera correlated with those in umbilical cord sera (r=0.649, P<0.01). These observations suggest that maternal blood test could be a useful assay for detecting fetal exposure to TCS and TCC, and high exposure to TCS may be potentially associated with increased risk for fetal malformations.

Triclocarban Influences Antibiotic Resistance and Alters Anaerobic Digester Microbial Community Structure.

Triclocarban (TCC) is one of the most abundant organic micropollutants detected in biosolids. Lab-scale anaerobic digesters were amended with TCC at concentrations ranging from the background concentration of seed biosolids (30 mg/kg) to toxic concentrations of 850 mg/kg to determine the effect on methane production, relative abundance of antibiotic resistance genes, and microbial community structure. Additionally, the TCC addition rate was varied to determine the impacts of acclimation time. At environmentally relevant TCC concentrations (max detect = 440 mg/kg), digesters maintained function. Digesters receiving 450 mg/kg of TCC maintained function under gradual TCC addition, but volatile fatty acid concentrations increased, pH decreased, and methane production ceased when immediately fed this concentration. The concentrations of the mexB gene (encoding for a multidrug efflux pump) were higher with all concentrations of TCC compared to a control, but higher TCC concentrations did not correlate with increased mexB abundance. The relative abundance of the gene tet(L) was greater in the digesters that no longer produced methane, and no effect on the relative abundance of the class 1 integron integrase encoding gene (intI1) was observed. Illumina sequencing revealed substantial community shifts in digesters that functionally failed from increased levels of TCC. More subtle, yet significant, community shifts were observed in digesters amended with TCC levels that did not inhibit function. This research demonstrates that TCC can select for a multidrug resistance encoding gene in mixed community anaerobic environments, and this selection occurs at concentrations (30 mg/kg) that can be found in full-scale anaerobic digesters (U.S. median concentration = 22 mg/kg, mean = 39 mg/kg).

Chinese population exposure to triclosan and triclocarban as measured via human urine and nails.

Triclosan (TCS) and triclocarban (TCC) exposures are highly concerned due to their suspected endocrine-disrupting effects. The present study investigated TCS and TCC exposure levels in the general Chinese population by biomonitoring human urine and nail samples. TCS (69-80%) and TCC (99-100%) were frequently detected, which demonstrates that the general Chinese population has extensive exposure to these chemicals. The geometric mean (GM) urinary concentrations were 0.40μg/g creatinine (creat), 95% confidence interval (CI) 0.30-0.56, for TCS and 0.40μg/g creat, 95% CI 0.29-0.56, for TCC. On the other hand, the GM levels of TCS and TCC were 13.57 (5.67μg/kg) and 84.66μg/kg (41.50μg/kg) in fingernail (toenail) samples, respectively, indicating that the levels in fingernails were approximately twice as high as those in toenails. Pearson's correlation coefficients between the urine and fingernail (toenail) samples were 0.715 (0.614) for TCS and 0.829 (0.812) for TCC. These data suggest that nail samples can be applied to the biomonitoring for TCS and TCC in the general population. We observed that the levels of both chemicals were higher in females than in males for urine and fingernail samples, but no significant differences were found between different genders for either compound in toenails. Nineteen- to 29-year-olds had the highest TCS levels in their nail samples, whereas TCC levels did not differ with regard to age. Region of residence significantly influenced TCS and TCC concentrations in the three biological matrices measured.

Occurrence and fate of triclosan and triclocarban in a subtropical river and its estuary

The occurrence of triclosan (TCS) and triclocarban (TCC) in a subtropical river (Jiulong River) and its estuary was investigated for two years. TCS and TCC were ubiquitously detected in the Jiulong River and its estuary. The levels of TCS and TCC ranged from less than the method detection limit to 64ng/L and from 0.05 to 14.1ng/L in the river, respectively. The levels of TCS and TCC in the estuary ranged from 2.56 to 27.25ng/L and 0.38 to 5.76ng/L, respectively. Temporal and spatial variations of TCS and TCC in the Jiulong River and its estuary were observed during the investigation. The weather conditions did not show significant correlations with TCS and TCC, whereas several water quality parameters showed high correlations with TCS and TCC. The microcosm studies showed that both direct photolysis and biodegradation contributed to TCS removal, whereas indirect photolysis was important for TCC removal in the surface water.

Human Fetal Exposure to Triclosan and Triclocarban in an Urban Population from Brooklyn, New York

Triclosan (TCS) and triclocarban (TCC) are antimicrobial agents formulated in a wide variety of consumer products (including soaps, toothpaste, medical devices, plastics, and fabrics) that are regulated by the U.S. Food and Drug Administration (FDA) and U.S. Environmental Protection Agency. In late 2014, the FDA will consider regulating the use of both chemicals, which are under scrutiny regarding lack of effectiveness, potential for endocrine disruption, and potential contribution to bacterial resistance to antibiotics. Here, we report on body burdens of TCS and TCC resulting from real-world exposures during pregnancy. Using liquid chromatography tandem mass spectrometry, we determined the concentrations of TCS, TCC, and its human metabolites (2′-hydroxy-TCC and 3′-hydroxy-TCC) as well as the manufacturing byproduct (3′-chloro-TCC) as total concentrations (Σ−) after conjugate hydrolysis in maternal urine and cord blood plasma from a cohort of 181 expecting mother/infant pairs in an urban multiethnic population from Brooklyn, NY recruited in 2007–09. TCS was detected in 100% of urine and 51% of cord blood samples after conjugate hydrolysis. The interquartile range (IQR) of detected TCS concentrations in urine was highly similar to the IQR reported previously for the age-matched population of the National Health and Nutrition Examination Survey (NHANES) from 2003 to 2004, but typically higher than the IQR reported previously for the general population (detection frequency = 74.6%). Urinary levels of TCC are reported here for the first time from real-world exposures during pregnancy, showing a median concentration of 0.21 μg/L. Urinary concentrations of TCC correlated well with its phase-I metabolite ∑-2′-hydroxy-TCC (r = 0.49) and the manufacturing byproduct ∑-3′-chloro-TCC C (r = 0.79), and ∑-2′-hydroxy-TCC correlated strongly with ∑-3′-hydroxy-TCC (r = 0.99). This human biomonitoring study presents the first body burden data for TCC from exposures occurring during pregnancy and provides additional data on composite exposure to TCS (i.e., from both consumer-product use and environmental sources) in the maternal–fetal unit for an urban population in the United States.

Occurrence, distribution, and multi-phase partitioning of triclocarban and triclosan in an urban river receiving wastewater treatment plants effluent in China

Occurrence, distribution, spatial and seasonal variations, and partitioning between aqueous phase and suspended particulate matters (SPM) of triclocarban (TCC) and triclosan (TCS) in Xiaoqing River, which receives wastewater treatment plant (WWTP) effluents, were studied. The distribution of the total TCC and TCS levels in surface water and sediments along the river were discussed. The highest TCC and TCS concentrations were both found near the discharge port of WWTPs, and the TCC and TCS levels decreased downstream of the WWTPs as a result of their distances from the source of WWTP discharges. The mean values of TCC and TCS in low-flow season were 1.62 and 1.80 times, respectively, as much as in high-flow season in surface water. The study on partitioning of TCC and TCS between aqueous phase and SPM shown the mean level of dissolved TCC accounted for about 10 % of the total level in surface water, whereas the TCS level was about 30 %. The TCC concentrations detected in the surface sediment samples (0 to 5 cm) ranged from 226 to 1,956 ng/g, with a mean value of 733 ng/g. The TCS levels were between 85 and 705 ng/g, with a mean value of 255 ng/g. The distribution and variations of TCC and TCS in sediments along the river were highly consistent with those in the water phase. The TCC and TCS levels in deep sediments (5 to 10 cm) were significantly lower than those in surface sediments. The mean TCC level in surface sediments was about 2.4 times as much as in deep sediments, and the TCS level in surface sediments was 3.1 times as much as in deep sediments.

The effect of liming on antibacterial and hormone levels in wastewater biosolids

This study analyzes the effect of liming on levels of triclocarban (TCC), triclosan (TCS), estrone (E1), and progesterone (P), two antimicrobial agents and two natural hormones, respectively. Factors studied include lime particle size, mixing time, and overall lime contact time. The study results suggest that coarse lime may be more active than fine lime due to less interaction with surrounding air. Both TCS and TCC concentrations were lower in coarse limed samples versus unlimed samples and the decrease was a function of time. A similar, but statistically insignificant trend in TCC and TCS levels was observed in fine lime samples with respect to unlimed samples. Liming was also found to decrease apparent E1 levels, with more notable decreases in samples amended with coarse lime. P-levels significantly increased after 1-day of contact time, stabilizing over the next 14 days of the study period. This increase and stabilization of P-levels was attributed to the pH and moisture-driven conversion of more chemically complex steroids into P.

Fate of Triclocarban, Triclosan and Methyltriclosan during wastewater and biosolids treatment processes

Triclocarban (TCC) and Triclosan (TCS) are two antibacterial chemicals present in household and personal care products. Methyltriclosan is a biodegradation product of TCS formed under aerobic conditions. TCC and TCS are discharged to Waste Water Treatment Plants (WWTP) where they are removed from the liquid phase mainly by concentrating in the solids. This study presents a thorough investigation of TCC, TCS and MeTCS concentrations in the liquid phase (dissolved + particulate) as well as solid phases within a single, large WWTP in the U.S. Total TCC and TCS concentrations decreased by >97% with about 79% of TCC and 64% of TCS transferred to the solids. The highest TCC and TCS removal rates from the liquid phase were reached in the primary treatment mainly though sorption and settling of solids. The TCC mass balances showed that TCC levels remain unchanged through the secondary treatment (activated sludge process) and about an 18% decrease was observed through the nitrification–denitrification process. On the other hand, TCS levels decreased in both processes (secondary and nitrification–denitrification) by 10.4 and 22.6%, respectively. The decrease in TCS levels associated with observed increased levels of MeTCS in secondary and nitrification–denitrification processes providing evidence of TCS biotransformation. Dissolved-phase concentrations of TCC and TCS remained constant during filtration and disinfection. TCC and TCS highest sludge concentrations were analyzed in the primary sludge (13.1 ± 0.9 μg g−1 dry wt. for TCC and 20.3 ± 0.9 μg g−1 dry wt. for TCS) but for MeTCS the highest concentrations were analyzed in the secondary sludge (0.25 ± 0.04 μg g−1 dry wt.). Respective TCC, TCS and MeTCS concentrations of 4.15 ± 0.77; 5.37 ± 0.97 and 0.058 ± 0.003 kg d−1 are leaving the WWTP with the sludge and 0.13 ± 0.01; 0.24 ± 0.07 and 0.021 ± 0.002 kg d−1 with the effluent that is discharged.

An Immunoassay To Evaluate Human/Environmental Exposure to the Antimicrobial Triclocarban

A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclocarban (TCC) was developed. The haptens were synthesized by derivatizing the para position of a phenyl moiety of TCC. The rabbit antisera were screened and the combination of antiserum 1648 and a heterologous competitive hapten containing a piperidine was further characterized. The IC(50) and detection range for TCC in buffer were 0.70 and 0.13-3.60 ng/mL, respectively. The assay was selective for TCC, providing only low cross-reactivity to TCC-related compounds and its major metabolites except for the closely related antimicrobial 3-trifluoromethyl-4,4'-dichlorocarbanilide. A liquid-liquid extraction for sample preparation of human body fluids resulted in an assay that measured low part per billion levels of TCC in small volumes of the samples. The limits of quantification of TCC were 5 ng/mL in blood/serum and 10 ng/mL in urine, respectively. TCC in human urine was largely the N- or N'-glucuronide. TCC concentrations of biosolids measured by the ELISA were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid, inexpensive, and convenient tool to aid researchers monitoring human/environmental exposure to TCC to better understand the health effects.