Tribulus Terrestris Extract Research Articles

The effect of Alcoholic plant (tribulus terrestris) extract on the bacteria isolated from genital tract of infertile cows in vitro

Out of Two hundred swabs collected from genital tract of infertile cows during the period between7/1/2012 to 30/6-2012. Cultured on Blood agar, Nutrient agar, MacConkey agar and Mannitol salt agar. 195 swab represented (97.5%) give positive result and 5 swab (2.5%) give negative result. from the positive culture 78 isolated (39%) mixed bacterial infection. All bacteria isolates were identified by using different biochemical tests and API-20E, the single bacterial isolation in this study were Esherichia coli 41.8%, Staphylococcus aureus36.8%, Pseudomonas aeruginosa17%, Citrobacter braakii1.7%, Providencia rettergi1.7% and Shewanella putrefaciens 0.9%. In mixed bacterial isolation, the highest percentage was 53.8% Staphylococcus + Esherishia coli aureus and the lowest percentage was 1.3% Esherishia coli + Citrobacter braakii + Pseudomonas aeruginosa. Antibiotic susceptibility test were carried out on bacterial isolates using 10 single antibiotic discs of commonly used drug, the test showed that most bacterial isolated resist for most antibiotics (Amikacin, Amoxicillin, Nalidix acid, (Amoxicillin and Clavulanic acid), Cefixime, Ceftriaxone, Tetracycline, Nitrofurantoin, Norfloxacin and Gentamycin) on the other hand observed sensitive for alcoholic plant tribulus terrestris extract (fruit) in different concentrations

861 Tribulus terrestris extract as curative agent in experimentally induced urolithiasis

861 Tribulus terrestris extract as curative agent in experimentally induced urolithiasis

The effect of the Tribulus terrestris extract on spermatogenesis in the rat

The effect of the Tribulus terrestris extract on spermatogenesis in the rat

Influence of Tribulus terrestris on testicular enzyme in fresh water ornamental fish Poecilia latipinna

The influence of Tribulus terrestris on the activities of testicular enzyme in Poecilia latipinna was assessed in lieu of reproductive manipulation. Different concentrations of (100, 150, 200, 250, and 300 mg) Tribulus terrestris extract and of a control were tested for testicular activity of enzymes in Poecilia latipinna for 2 months. The testis and liver were homogenized separately in 0.1 mol/l potassium phosphate buffer (0.1 mol/l, pH 7.2). The crude homogenate was centrifuged, and supernatant obtained was used as an enzyme extract for determination of activities. The activities of testicular functional enzyme ALP, ACP, SDH, LDH, and G6PDH levels were changed to different extent in treated groups compared with that of the control. The total body weight and testis weight were increased with the Tribulus terrestris-treated fish (Poecilia latipinna). These results suggest that Tribulus terrestris induced the testicular enzyme activity that may aid in the male reproductive functions. It is discernible from the present study that Tribulus terrestris has the inducing effect on reproductive system of Poecilia latipinna.

Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribulus terrestris

Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate solution, using phytotherapy is being sought. The present study attempted to evaluate the antilithiatic properties of Tribulus terrestris commonly called as "gokhru" which is often used in ayurveda to treat various urinary diseases including urolithiasis. The activity of Tribulus terrestris was investigated on nucleation and the growth of the calcium oxalate (CaOx) crystals as well as on oxalate induced cell injury of NRK 52E renal epithelial cells. Tribulus terrestris extract exhibited a concentration dependent inhibition of nucleation and the growth of CaOx crystals. When NRK-52E cells were injured by exposure to oxalate for 72 h, Tribulus terrestris extract prevented the injury in a dose-dependent manner. On treatment with the different concentrations of the plant, the cell viability increased and lactate dehydrogenase release decreased in a concentration dependent manner. The current data suggests that Tribulus terrestris extract not only has a potential to inhibit nucleation and the growth of the CaOx crystals but also has a cytoprotective role. Our results indicate that it could be a potential candidate for phytotherapy against urolithiasis.

Effects of Tribulus terrestris (Puncture Vine) supplementation on performance and digestive system of broiler chicks

The purpose of this study was to determine the possible effects of the herbal preparation Tribulus terrestris (TT) extract on muscle building (increase in breast and legs weight) in broiler chicks. One hundred eighty broiler chicks were assigned to three treatment groups; control, 180 ppm TT and 360 ppm TT extract. Body weight, body gain and dietary intake were determined weekly for 6 weeks. Control diet included 234 g crude protein (CP) and 3141 kcal ME (grower) per kg diet during 1-21 days of age and 204 g CP with 3199 kcal ME (finisher) per kg diet for 22-42 days of age. Growth performance, muscle building (increase in breast and legs weights) and digestive system parts were determined. TT extract did not affect growth performance and muscle building but decreased ileum + jejunum and empty gut weights (P<0.05). In conclusion, the present results showed that Tribulus terrestris had no effect on growth or muscle building. This may be TT extract do not work in birds as do in human subjects, and more likely, the physiological differences between human and bird.

Effects of Tribulus terrestris on endocrine sensitive organs in male and female Wistar rats

Aim of the study Investigate the possible effects of Tribulus terrestris (TT) on endocrine sensitive organs in intact and castrated male rats as well as in a post-menopausal rat model using ovariectomized females. Materials and methods Three different dose levels of TT (11, 42 and 110 mg/kg/day) were administered to castrated males for 7 days and to intact males and castrated females for 28 days. In addition to TT treatment, all experiments also included a group of rats treated with dehydroepiandrosterone (DHEA). In experiments using castrated males and females we also used testosterone and 17α-ethynylestradiol, respectively, as positive controls for androgenicity and estrogenicity. Results Neither DHEA nor TT was able to stimulate androgen sensitive tissues like the prostate and seminal vesicle in both intact and castrated male rats. In addition, administration of TT to intact male rats for 28 days did not change serum testosterone levels as well as did not produce any quantitative change in the fecal excretion of androgenic metabolites. However, a slight increase in the number of homogenization-resistant spermatids was observed in rats treated with 11 mg/kg/day of TT extract. In ovariectomized females, TT did not produce any stimulatory effects in uterine and vaginal epithelia. Conclusions Tribulus terrestris was not able to stimulate endocrine sensitive tissues such as the prostate, seminal vesicle, uterus and vagina in Wistar rats, indicating lack of androgenic and estrogenic activity in vivo. We also showed a positive effect of TT administration on rat sperm production, associated with unchanged levels of circulating androgens.

Changes in the brain cortex of rabbits on a cholesterol-rich diet following supplementation with a herbal extract of Tribulus terrestris.

Extracts of the medicinal herb Tribulus terrestris (TT) are used for treating various diseases. The saponins, a component of TT, play a role in regulating blood pressure and in treatment of hyperlipidemia. The aim of the study was to investigate the immunohistochemical and ultrastructural alterations in the cerebral cortex of experimental rabbits on a cholesterol rich diet treated with TT. The rabbits were divided into three groups and followed for 12 weeks as control group (CG); experimental group I (EG-I), fed with a cholesterol-rich diet; experimental group II (EG-II), treated with an extract of TT (5 mg/kg/day) after a cholesterol-rich diet of 4 weeks. In EG-I there were ultrastructural changes, including mitochondrial degeneration, increased lipofuscin pigments, myelin sheath damage with axoplasmic shrinkage and electron dense granules in the neurovascular unit. The number of synapses apparently decreased in both experimental groups. Administration of TT extract in EG-II led to marked ultrastructural alterations in neurons, including decreased mitochondrial degeneration (P<0.001) and extensive oedematous areas in the neurovascular unit. However, in EG-II, lamellar myelin, axonal structures and mitochondria were well protected. These alterations possibly indicate that saponins have an effect on the neurons either directly or by its conversion to steroidal saponins. Therefore, these findings add further evidence supporting the protective claims of TT in cerebral architecture in dietary induced hyperlipidemia.

Effects of Puncturevine (Tribulus terrestris) extract on spermatogenesis in the rat

Tribulus terrestris (Zygophyllaceae) have been used as an aphrodisiac both in the Indian and Chinese traditional systems of medicine. Administration of Tribulus terrestris extract (TT) increased sexual behavior and intracavernous pressure both in normal and castrated rats and these effects were probably due to the androgen increasing property of TT. The objective of the present study is to evaluate the effect of TT on the spermatogenesis in rat. 66 sexuality mature male rats with 320 to 370 weight were used and were divided into three groups .Group 1 was treated with distilled water and Group 2 and 3 was treated with TT at the dose of 10 and 20 mg/kg body weight orally, respectively, once daily for 2,3,4,5,and 7 weeks. The number of caudal epididymal sperm and weight of the epididym and testis were increased significantly at groups of 10 and 20 mg/kg for 28 and 35 days (p

The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction – an evaluation using primates, rabbit and rat

Hormonal effects of Tribulus terrestris (TT) were evaluated in primates, rabbit and rat to identify its usefulness in the management of erectile dysfunction (ED). TT extract was administered intravenously, as a bolus dose of 7.5, 15 and 30 mg/kg, in primates for acute study. Rabbits and normal rats were treated with 2.5, 5 and 10mg/kg of TT extract orally for 8 weeks, for chronic study. In addition, castrated rats were treated either with testosterone cypionate (10mg/kg, subcutaneously; biweekly for 8 weeks) or TT orally (5mg/kg daily for 8 weeks). Blood samples were analyzed for testosterone (T), dihydrotestosterone (DHT) and dehydroepiandrosterone sulphate (DHEAS) levels using radioimmunoassay. In primates, the increases in T (52%), DHT (31%) and DHEAS (29%) at 7.5mg/kg were statistically significant. In rabbits, both T and DHT were increased compared to control, however, only the increases in DHT (by 30% and 32% at 5 and 10mg/kg) were statistically significant. In castrated rats, increases in T levels by 51% and 25% were observed with T and TT extract respectively that were statistically significant. TT increases some of the sex hormones, possibly due to the presence of protodioscin in the extract. TT may be useful in mild to moderate cases of ED.

Masculinization of Convict Cichlid (Cichlasoma nigrofasciatum) by immersion in Tribulus terrestris extract

We have examined the effects of Tribulus terrestris (TT), a non-toxic herb, on sex reversal in the Convict Cichlid Cichlasoma nigrofasciatum with the aim of introducing a new environmentally friendly method for masculinization in C. nigrofasciatum. TT is a natural plant product that elevates the testosterone levels in humans and animals. Different concentrations (0.0, 0.10, 0.20 and 0.30 g/l) of TT extract were tested for their effect on sex reversal in C. nigrofasciatum by immersing newly hatched offspring once weekly for 2 months in TT extract. Of the dosages used in the present study, 0.30 g/l TT was the most effective in terms of masculinization, resulting in a maximum male ratio of 87.23% (P 0.05). We concluded that TT had no negative effect on the survival rate of C. nigrofasciatum. In addition, all groups of TT-treated fish exhibited successful growth acceleration compared to the control group, but only the 0.30 g/l TT treatment significantly improved the growth rate of C. nigrofasciatum. (P < 0.01). Sex reversal in C. nigrofasciatum demonstrated that TT-treated 0-day-old larvae showed successful sex reversal, spermatogenesis and a better growth rate than untreated progenies.

The Effects of Gokshura, Tribulus terrestris on Sex Reversal of Guppy, Poecilia reticulata

This study examined the effects of Tribulus terrestris (TT) on sex reversal in guppy, Poecilia reticulata. The objective of this study was to introduce a new environmentally friendly method for masculinization in P. reticulata. Since male guppy has higher commercial value than female. TT is a natural, non-toxic herb which helps enhance testosterone levels in human and animals. It was prepared in a laboratory in France. Different concentration (0.0, 0.05, 0.1 and 0.15 g L(-1)) of TT was investigated for sex reversal in the Poecilia reticulata. TT extract was administered by immersion of newly born offspring once weekly for two months. Among the dosages used in the present study 0.15 g L(-1) TT was the most effective dosage that ensured maximum male ratio (80%, p < 0.01). Although, sex ratios of 0.05 and 0.1 g L(-1) TT were not significantly different from the expected 1:1 ratio, in these two groups treatment with TT also result in higher number of males (58.25 and 59.77%, respectively), than control (p > 0.05). Total survival rates in all treatments and control were uniformly high ranging from 83 to 87% (p > 0.05). It is concluded that TT has no negative effect on survival rate of P. reticulata. All groups of TT-treated fish exhibited successful growth acceleration comparing to the control group, but only TT treatment at the concentration of 0.15 and 0.1 g L(-1) TT significantly improved growth rate of P. reticulata (p < 0.01). Histological examinations revealed that testes of fish treated with TT-extract contained all stages of spermatogenesis. Sex reversal in P. reticulata demonstrated that TT treated new-born progenies showed successful sex reversal, spermatogenesis and better growth rate than untreated progenies.

85 The effect of a tribulus terrestris extract on plasma steroid hormone concentrations and the urinary testosterone/epitestosterone (TE) ratio

85 The effect of a tribulus terrestris extract on plasma steroid hormone concentrations and the urinary testosterone/epitestosterone (TE) ratio

Effect of Tribulusterrestris on nicotinamide adenine dinucleotide phosphate-diaphorase activity and androgen receptors in rat brain

Tribulus terrestris L. (Zygophyllaceae) have been used as an aphrodisiac both in the Indian and Chinese traditional systems of medicine. Administration of Tribulus terrestris extract (TT) increased sexual behaviour and intracavernous pressure both in normal and castrated rats and these effects were probably due to the androgen increasing property of TT. The objective of the present study is to evaluate the effect of TT on nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity and androgen receptor (AR) immunoreactivity in rat brain. Twenty-four adult male Sprague-Dawley rats were divided into two groups of twelve each. Group I was treated with distilled water and Group II was treated with TT at the dose of 5mg/kg body weight orally, once daily for 8 weeks. Following treatment transcardiac perfusion was done with Ringer lactate, 4% paraformaldehyde and 30% sucrose. The brain tissue was removed and sections of the paraventricular (PVN) area of hypothalamus were taken for NADPH-d and AR immunostaining. There was an increase in both NADPH-d (67%) and AR immunoreactivity (58%) in TT treated group and these results were statistically significant compared to the control. Chronic treatment of TT in rats increases the NADPH-d positive neurons and AR immunoreactivity in the PVN region. Androgens are known to increase both AR and NADPH-d positive neurons either directly or by its conversion to oestrogen. The mechanism for the observed increase in AR and NADPH-d positive neurons in the present study is probably due to the androgen increasing property of TT. The findings from the present study add further support to the aphrodisiac claims of TT.

Natural Ways to Enhance Male Fertility

Alternative and Complementary TherapiesVol. 10, No. 1 ArticlesNatural Ways to Enhance Male FertilityChris D. Meletis and Jason BarkerChris D. MeletisSearch for more papers by this author and Jason BarkerSearch for more papers by this authorPublished Online:5 Jul 2004https://doi.org/10.1089/107628004772830366AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail FiguresReferencesRelatedDetailsCited byUlva lactuca methanolic extract improves oxidative stress-related male infertility induced in experimental animals31 July 2019 | Archives of Physiology and Biochemistry, Vol. 127, No. 5Evaluation of the Spermatogenic Activity of Polyherbal Formulation in Oligospermic MalesBioMed Research International, Vol. 2018EVALUATION OF THE EFFECTS OF CAPSULES CONTAINING TRIBULUS TERRESTRIS EXTRACT AND LCARNITINE ON TREATMENT OF OLIGOSPERMIA IN MALES14 July 2018 | Journal of Evolution of Medical and Dental Sciences, Vol. 7, No. 29Phytochemical and Biological Studies of Tribulus terrestris L. Growing in EgyptInternational Journal of Pharmacology, Vol. 14, No. 2Optimizing Female Fertility Chris Meletis and Jason Barker5 July 2004 | Alternative and Complementary Therapies, Vol. 10, No. 2 Volume 10Issue 1Feb 2004 To cite this article:Chris D. Meletis and Jason Barker.Natural Ways to Enhance Male Fertility.Alternative and Complementary Therapies.Feb 2004.22-27.http://doi.org/10.1089/107628004772830366Published in Volume: 10 Issue 1: July 5, 2004PDF download

Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats

Tribulus terrestris (TT) has long been used in the traditional Chinese and Indian systems of medicine for the treatment of various ailments and is popularly claimed to improve sexual functions in man. Sexual behaviour and intracavernous pressure (ICP) were studied in both normal and castrated rats to further understand the role of TT containing protodioscin (PTN) as an aphrodisiac. Adult Sprague-Dawley rats were divided into five groups of 8 each that included distilled water treated (normal and castrated), testosterone treated (normal and castrated, 10 mg/kg body weight, subcutaneously, bi-weekly) and TT treated (castrated, 5 mg/kg body weight, orally once daily). Decreases in body weight, prostate weight and ICP were observed among the castrated groups of rats compared to the intact group. There was an overall reduction in the sexual behaviour parameters in the castrated groups of rats as reflected by decrease in mount and intromission frequencies (MF and IF) and increase in mount, intromission, ejaculation latencies (ML, IL, EL) as well as post-ejaculatory interval (PEI). Compared to the castrated control, treatment of castrated rats (with either testosterone or TT extract) showed increase in prostate weight and ICP that were statistically significant. There was also a mild to moderate improvement of the sexual behaviour parameters as evidenced by increase in MF and IF; decrease in ML, IL and PEI. These results were statistically significant. It is concluded that TT extract appears to possess aphrodisiac activity probably due to androgen increasing property of TT (observed in our earlier study on primates).