The anti-collision pier plays an irreplaceable role in road traffic protection due to its significance. In this research, the biomimetic honeycomb structure was applied to internal anti-collision pier interior structures. The enhancement of mechanical strength and energy absorption characteristics was explored and optimized by five anti-collision pier honeycomb structures. Finite elements of the piers are designated as 650 mm in diameter and 850 mm in height. Polypropylene Acetate (PLA) material is utilized in this research due to its environment-friendly characteristics. Displacement loading in finite element simulation is 50 mm to the middle region of the model at YOZ direction. The energy-absorbing properties of five optimized honeycomb anti-collision piers at the same force position will be carefully compared. Moreover, the influence of internal hexagon direction-quantity configuration upon loading resistance under displacement loading is outlined. The results determined the best biomimetic structure to be three honeycomb shapes with a central triangle area, with maximum stress of 503.8 MPa and fracture displacement of 58.02 mm. Furthermore, the numerical simulation shows that the number of nest increases has a negative relationship with the effect upon force and deformation of the model. Moreover, the triangular central area is superior to the Y-shape central area in both mechanical strength and energy absorption performance.