Triacylglyceride Content Research Articles

Effect of erva-mate (Ilex paraguariensis A. St.-Hil., Aquifoliaceae) on serum cholesterol, triacylglycerides and glucose in Wistar rats fed a diet supplemented with fat and sugar

Ilex paraguariensis A. St.-Hil., Aquifoliaceae, is a species native to the subtropical and temperate regions of South America, used in beverages prepared by infusion such as teas, chimarrão and tererê. To investigate the physiological effects of I. paraguariensis on the metabolism of fats and sugars in Wistar rats, following the ingestion of erva-mate tea, four experimental groups were constructed: Lipid Control Group (receiving water and high-fat diet); Lipid Tea Group (extract of I. paraguariensis and high-fat diet); the Sugar Control Group (water and high-sugar diet); and Sugar Tea Group (extract of I. paraguariensis and high-sugar diet). The animals received their particular diet for 60 days, and were weighed weekly. After this period, the plasma concentrations of cholesterol, glucose and triacylglycerides were determined, together with the weight of visceral fat. The data were subjected to statistical analysis with a significance level of p<0.05. The results show that the ingestion of erva-mate affected body weight, visceral fat and plasma glucose, cholesterol and triacylglyceride levels.

Sample classification for improved performance of PLS models applied to the quality control of deep-frying oils of different botanic origins analyzed using ATR-FTIR spectroscopy

The selection of an appropriate calibration set is a critical step in multivariate method development. In this work, the effect of using different calibration sets, based on a previous classification of unknown samples, on the partial least squares (PLS) regression model performance has been discussed. As an example, attenuated total reflection (ATR) mid-infrared spectra of deep-fried vegetable oil samples from three botanical origins (olive, sunflower, and corn oil), with increasing polymerized triacylglyceride (PTG) content induced by a deep-frying process were employed. The use of a one-class-classifier partial least squares-discriminant analysis (PLS-DA) and a rooted binary directed acyclic graph tree provided accurate oil classification. Oil samples fried without foodstuff could be classified correctly, independent of their PTG content. However, class separation of oil samples fried with foodstuff, was less evident. The combined use of double-cross model validation with permutation testing was used to validate the obtained PLS-DA classification models, confirming the results. To discuss the usefulness of the selection of an appropriate PLS calibration set, the PTG content was determined by calculating a PLS model based on the previously selected classes. In comparison to a PLS model calculated using a pooled calibration set containing samples from all classes, the root mean square error of prediction could be improved significantly using PLS models based on the selected calibration sets using PLS-DA, ranging between 1.06 and 2.91% (w/w).

Lipidomics Analysis Reveals Efficient Storage of Hepatic Triacylglycerides Enriched in Unsaturated Fatty Acids after One Bout of Exercise in Mice

BackgroundEndurance exercise induces lipolysis, increases circulating concentrations of free fatty acids (FFA) and the uptake and oxidation of fatty acids in the working muscle. Less is known about the regulation of lipid metabolism in the liver during and post-exercise.Methodology/Principal FindingsWe performed an ultra fast liquid chromatography-mass spectrometry (UFLC-MS) based lipidomics analysis of liver tissue samples obtained from C57Bl/6J mice immediately after a 60 min treadmill run of moderate intensity, and after 3 h of recovery. The PLS-DA scores plot for 115 quantified lipid molecular species revealed a clear separation of the hepatic lipid profile of sedentary from recovering mice, but not from mice immediately after running. 21 lipid species were considered to be most responsible for the difference in the hepatic lipid profiles, including 17 triacylglycerides (TG), one lysophosphatidylcholine (LPC) and three phosphatidylcholines (PC). TG species were found to be more abundant in the recovery phase, while PC species were decreased. The degree of accumulation of individual TG species correlated well with the amount of theoretical energy stored whereas no increase was found for TG species containing only saturated or one monounsaturated fatty acid. Total liver TG content as assayed by an enzymatic method was increased to 163% in the recovery phase, while it was significantly decreased in skeletal muscle by the exercise bout and remained less in the recovery phase. Results from fasted and refed mice indicate that fasting-induced lipolysis was associated with a pronounced accumulation of hepatic TG, which is reversed by refeeding for 5 h. Thus food intake per se did not elevate hepatic TG.ConclusionThese data indicate that high availability of FFA induced by endurance exercise or fasting resulted in a transient hepatic TG accumulation, while muscle TG content was decreased during exercise presumably due to increased muscle fatty acid oxidation.

Early Impairment of Transmural Principal Strains in the Left Ventricular Wall After Short-Term, High-Fat Feeding of Mice Predisposed to Cardiac Steatosis

myocardial lipid accumulation precedes some cardiomyopathies, but little is known of concurrent effects on ventricular mechanics. We tested the hypothesis that intramyocardial lipid accumulation during a short-term, high-fat diet (HFD) affects 2-dimensional strains in the heart. We examined the hearts of nontransgenic (NTG) mice and of transgenic mice predisposed to elevated triacylglyceride (TAG) storage linked to low-level overexpression of peroxisome proliferator activated receptor (PPAR-α). myocardial lipid and transmural principal strains E1 and E2 were determined in vivo with (1)H magnetic resonance spectroscopy/imaging before and after 2 weeks of an HFD in both PPAR-α and NTG littermate mice. Baseline lipid was elevated in PPAR-α compared with NTG mice. An HFD increased mobile lipid by 174% in NTG mice (P<0.05) and by 79% in PPAR-α mice (P<0.05). After an HFD, lipid and TAG were higher in PPAR-α versus NTG mice by 63% and 81%, respectively. However, TAG in PPAR-α mice after an HFD was similar to TAG in PPAR-α mice fed a regular diet, suggesting that the magnetic resonance spectroscopy signal from lipid is not exclusive to TAG. Only at the highest lipid contents, achieved in PPAR-α mice, were strains affected. Endocardial strain was most compromised, with a negative correlation to lipid (P<0.05). a short-term HFD elevated myocardial lipid measures as determined by magnetic resonance spectroscopy, which became dissociated from TAG content in hearts predisposed to cardiac steatosis. The increased lipid was associated with concurrent, transmural reductions in E1 and E2 strains across the left ventricular wall. Strains were attenuated at the highest levels of lipid accumulation, suggesting a threshold response. Thus, 2-dimensional strains are impaired early and without left ventricular diastolic dysfunction, owing to cardiac steatosis.

Palmitate protects hepatocytes from oxidative stress and triacylglyceride accumulation by stimulation of nitric oxide synthesis in the presence of high glucose and insulin concentration

Excessive flux of free fatty acids (FFA) into the liver contributes to liver impairment in non-alcoholic fatty liver disease (NAFLD). It remains unclear how FFA contribute to impairment of hepatocytes. This study treated hepatocytes with linoleic acid and palmitate to investigate the early event triggering FFA-mediated impairment. It determined cell viability, content of nitrite/nitrate and triacylglycerides (TG), inducible nitric oxide synthase (iNOS) protein, oxidation of cardiolipin (CL) as well as formation of F2-isoprostanes in the presence of insulin and glucose. Linoleic acid caused significant decrease in cell viability. It is shown that palmitate caused induction of iNOS resulting in increased nitrite/nitrate concentration and slight increase in TG content. Linoleic acid led to a decrease in nitrite/nitrate concentration parallelled by massive TG accumulation in combination with increased oxidation of CL and increased F2-isoprostane levels. It is concluded that nitric oxide (NO) concentration regulates FFA-dependent TG accumulation and oxidative stress in rat hepatocytes.

Effect of inulin supplementation and dietary fat source on performance, blood serum metabolites, liver lipids, abdominal fat deposition, and tissue fatty acid composition in broiler chickens

A study was conducted to evaluate the effect of adding inulin to diets containing 2 different types of fat as energy sources on performance, blood serum metabolites, liver lipids, and fatty acids of abdominal adipose tissue and breast and thigh meat. A total of 240 one-day-old female broiler chicks were randomly allocated into 1 of 6 treatments with 8 replicates per treatment and 5 chicks per pen. The experiment consisted of a 3 x 2 factorial arrangement of treatments including 3 concentrations of inulin (0, 5, and 10 g/kg of diet) and 2 types of fat [palm oil (PO) and sunflower oil (SO)] at an inclusion rate of 90 g/kg of diet. The experimental period lasted from 1 to 34 d. Dietary fat type did not affect BW gain but impaired feed conversion (P < 0.001) in birds fed the PO diets compared with birds fed the SO diets. The diets containing PO increased abdominal fat deposition and serum lipid and glucose concentrations. Triacylglycerol contents in liver were higher in the birds fed PO diets. Dietary fat type also modified fatty acids of abdominal and i.m. fat, resulting in a higher concentration of C16:0 and C18:1n-9 and a lower concentration of C18:2n-6 in the birds fed PO diets. The addition of inulin to diets modified (P = 0.017) BW gain quadratically without affecting feed conversion. Dietary inulin decreased the total lipid concentration in liver (P = 0.003) and that of triacylglycerols and very low density lipoprotein cholesterol (up to 31%) in blood serum compared with the control groups. The polyunsaturated fatty acid:saturated fatty acid ratio increased in abdominal and i.m. fat when inulin was included in the SO-containing diets. The results from the current study suggest that the addition of inulin to broiler diets has a beneficial effect on blood serum lipids by decreasing triacylglyceride concentrations The results also support the use of inulin to increase the capacity of SO for enhancing polyunsaturated fatty acid:saturated fatty acid ratio of i.m. fat in broilers.

Supercritical fluid extraction of grape seed ( Vitis vinifera L.) oil. Effect of the operating conditions upon oil composition and antioxidant capacity

In this work, the supercritical fluid extraction of grape seed oil has been studied at 180, 200 and 220 bar, and 313.15 and 323.15 K to analyse their influence upon oil quality. The extraction curves exhibit an initial linear extraction period followed by a smooth asymptotic plateau at 11.5%. The extraction rate increases with increasing pressure and decreasing temperature, due to the influence of pressure and temperature upon the oil solubility; the convective mass transfer coefficient played a minor role on the process. The triacylglycerides content and the fatty acids profile have been determined, being roughly unaffected by the operating conditions adopted. The antioxidant activity of the oil has been assessed by the DPPH spectrophotometric method, and showed to increase with pressure and noticeably with temperature. Along extraction curves, the antioxidant activity is more pronounced on the oil collected during the first stages of the process, where 35–40% of total oil is extracted.

Influence of nitrogen-limitation regime on the production by Chlorella vulgaris of lipids for biodiesel feedstocks

Microalgae have the potential to provide large amounts of lipid-containing biomass that could be used as a renewable oil feedstock for biofuel use. Many fast-growing algae, such as Chlorella vulgaris, when grown under nutrient-sufficient conditions, have a lipid profile that is unsuitable for biodiesel use, since the triacylglyceride content is too low. However, growth under nitrogen limitation has been shown to increase triacylglycerides. Here we investigate the most effective nitrogen-limitation regime and find that rather than transferring cells to a medium without nitrogen, the maximal triacylglyceride productivity (46 mg/l/day) is achieved by allowing the cells to deplete the nitrogen naturally. Detailed analysis of the fatty acid composition of the lipids indicated that there was a high proportion of α-linolenic acid (C18:3) under all conditions, so that it would have to be blended with other feedstocks in order to adhere to international standards for biodiesel.

Monitoring of Polymerized Triglycerides in Deep-Frying Oil by On-Line GPC-FTIR Spectrometry Using the Science Based Calibration Multivariate Approach

On-line gel permeation chromatography Fourier transform infrared (GPC-FTIR) is proposed as detection technique for the determination of the total polymer triacylglyceride (PTG) content in olive oil as an alternative to other conventional detectors such as the evaporative light scattering detector (ELSD) or the refraction index detector (RID). FTIR detection allowed confirmation additionally to quantification of the analytes via their characteristic infrared spectra. For the extraction of chromatograms, a multivariate method called science based calibration (SBC) was used. The SBC method employed a reference spectrum of triacylglycerides (TG) extracted from the injection of a fresh olive oil sample and data obtained from a blank injection as ‘noise’ matrix, to estimate a regression vector subsequently used to predict the relative concentration of the analyte during the GPC run. Results found evidenced that the use of SBC improved the selectivity, sensitivity and repeatability of the GPC-FTIR measurements as compared to the ‘classical’ approach based on the measurement of the spectral area in a defined spectral range. The developed method provided a limit of detection for PTGs of 0.19 mg mL−1, which corresponds to a 0.65% w/w for an oil sample mass of 300 mg.

Correction

HomeCirculation ResearchVol. 104, No. 9Correction Free AccessCorrectionPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toFree AccessCorrectionPDF/EPUBCorrection Originally published8 May 2009https://doi.org/10.1161/CIRCRESAHA.109.100005Circulation Research. 2009;104:e59This article corrects the followingSubstrate–Enzyme Competition Attenuates Upregulated Anaplerotic Flux Through Malic Enzyme in Hypertrophied Rat Heart and Restores Triacylglyceride ContentIn the article by Pound et al, published in the March 27, 2009, issue of the journal (Circ Res. 2009;104:805–812), Figure 4 incorrectly shows the magnitude of flux values in the y axis. In the published graph, y axis values incorrectly extend from 0 to 1.2. The corrected scale extends from 0 to 0.12. The relative differences between experimental groups remain as indicated in the figure legend and article text, and the conclusions of the study are not affected. The corrected figure and corresponding legend are provided below, and the corrected article can be found online at http://circres.ahajournals.org/cgi/content/full/104/6/805. Download figureDownload PowerPointFigure 4. Fractional contribution of anaplerotic flux to citric acid cycle flux. Note elevated anaplerosis in hypertrophied rat hearts (HYP). Activation of PDH to compete against increased malic enzyme in hypertrophied hearts (HYP+DCA) moderated anaplerosis, which was lower than in untreated HYP. Black bar indicates sham hearts; white bar, DCA-treated sham hearts; hatched bar, hypertrophied hearts; gray bar, DCA-treated hypertrophied hearts. *P<0.05 vs the other 3 groups; †P<0.05 vs HYP.The authors regret this error.Reference1. Pound KM, Sorokina N, Ballal K, Berkich DA, Fasano M, LaNoue KF, Taegtmeyer H, O'Donnell JM, Lewandowski ED: Substrate–enzyme competition attenuates upregulated anaplerotic flux through malic enzyme in hypertrophied rat heart and restores triacylglyceride content: attenuating upregulated anaplerosis in hypertrophy. Circ Res. 2009;104:805–812. Previous Back to top Next FiguresReferencesRelatedDetailsRelated articlesSubstrate–Enzyme Competition Attenuates Upregulated Anaplerotic Flux Through Malic Enzyme in Hypertrophied Rat Heart and Restores Triacylglyceride ContentKayla M. Pound, et al. Circulation Research. 2009;104:805-812 May 8, 2009Vol 104, Issue 9 Advertisement Article InformationMetrics https://doi.org/10.1161/CIRCRESAHA.109.100005 Originally publishedMay 8, 2009 PDF download Advertisement

Omega-3 oil intake during weight loss in obese women results in remodelling of plasma triglyceride and fatty acids.

Previous studies have shown that a combination of weight loss and fish oil supplementation reduce cardiovascular disease and diabetes risks by increasing adiponectin and reducing triacylglyceride concentrations, while weight loss alone significantly improves insulin sensitivity and reduces inflammation. Here, a metabolomic approach, using a combination of 1H-Nuclear Magnetic Resonance spectroscopy, and gas and liquid chromatography and mass spectrometry, was employed to elucidate the metabolic changes in blood plasma following weight loss and fish oil supplementation. The intervention study was conducted over 24 weeks, with 93 female subjects randomised to one of three groups. Two groups followed a 12-week weight loss program, followed by a 12-week weight maintenance period and were randomised to fish or placebo oil capsules; a control group did not follow the weight loss program and were given placebo oil capsules. Lipid profiles changed dramatically upon fish oil intake and subtly across the two weight loss groups. While the fish oil supplementation increased the proportion of various phospholipid species, previously reported reductions in total triacylglycerides (TAGs) upon fish oil intake were shown to be driven by a reduction in a specific subset of the measured TAGs. This remodelling of triglycerides may represent further beneficial effects of fish oil supplementation.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-009-0161-7) contains supplementary material, which is available to authorized users.

Substrate–Enzyme Competition Attenuates Upregulated Anaplerotic Flux Through Malic Enzyme in Hypertrophied Rat Heart and Restores Triacylglyceride Content

Recent work identifies the recruitment of alternate routes for carbohydrate oxidation, other than pyruvate dehydrogenase (PDH), in hypertrophied heart. Increased carboxylation of pyruvate via cytosolic malic enzyme (ME), producing malate, enables "anaplerotic" influx of carbon into the citric acid cycle. In addition to inefficient NADH production from pyruvate fueling this anaplerosis, ME also consumes NADPH necessary for lipogenesis. Thus, we tested the balance between PDH and ME fluxes in hypertrophied hearts and examined whether low triacylglyceride (TAG) was linked to ME-catalyzed anaplerosis. Sham-operated (SHAM) and aortic banded rat hearts (HYP) were perfused with buffer containing either 13C-palmitate plus glucose or (13)C glucose plus palmitate for 30 minutes. Hearts remained untreated or received dichloroacetate (DCA) to activate PDH and increase substrate competition with ME. HYP showed a 13% to 26% reduction in rate pressure product (RPP) and impaired dP/dt versus SHAM (P<0.05). DCA did not affect RPP but normalized dP/dt in HYP. HYP had elevated ME expression with a 90% elevation in anaplerosis over SHAM. Increasing competition from PDH reduced anaplerosis in HYP+DCA by 18%. Correspondingly, malate was 2.2-fold greater in HYP than SHAM but was lowered with PDH activation: HYP=1419+/-220 nmol/g dry weight; HYP+DCA=343+/-56 nmol/g dry weight. TAG content in HYP (9.7+/-0.7 micromol/g dry weight) was lower than SHAM (13.5+/-1.0 micromol/g dry weight). Interestingly, reduced anaplerosis in HYP+DCA corresponded with normalized TAG (14.9+/-0.6 micromol/g dry weight) and improved contractility. Thus, we have determined partial reversibility of increased anaplerosis in HYP. The findings suggest anaplerosis through NADPH-dependent, cytosolic ME limits TAG formation in hypertrophied hearts.

Single strand conformation polymorphism in intron I of the chicken apoVLDL-II gene, and its relationship with triglyceride and very low density lipoprotein concentrations

Genetically fat and lean chickens reared identically in the same environment were used to investigate the effect of the apoVLDL-II gene (intron I) on total cholesterol, triglyceride, and lipoprotein concentrations. The overall allele frequency in the 2 populations was 0.59 for allele A and 0.41 for allele B. The allele frequency in the Rugao breed was 0.52 for allele A and 0.48 for allele B; however, in the Anka breed it was 0.65 for allele A and 0.35 for allele B. The apoVLDL-II genotype in the genetically fat Anka breed was associated with a significant increase in serum triacylglyceride concentration in sexually immature birds.

Impact of ovariohysterectomy and food intake on body composition, physical activity, and adipose gene expression in cats1

The mechanisms contributing to BW gain following ovariohysterectomy in domestic cats are poorly understood. Moreover, the effects of food restriction to maintain BW following spaying have been poorly studied. Thus, our primary objective was to determine the effects of spaying and food restriction to maintain BW on adipose and skeletal muscle mRNA abundance and activity levels in cats. After a 4-wk baseline period (wk 0), 8 adult (approximately 1.5 yr old) domestic shorthair cats were spayed and fed to maintain BW for 12 wk. After 12 wk, cats were fed ad libitum for an additional 12 wk. Body composition was determined, activity levels were measured, and adipose and muscle biopsies were collected at wk 0, 12, and 24. Fasting blood samples were collected at wk 0, 6, 12, 18, and 24. To maintain BW post-spay, food intake was decreased (P < 0.05) by 30%. During this phase, mRNA abundance of adipose tissue lipoprotein lipase and leptin was decreased (P < 0.05), representing only 52 and 23% of baseline expression, respectively. Interleukin-6 mRNA, however, was increased (P < 0.05) 2-fold. Physical activity was decreased (P < 0.05) by wk 12, most dramatically during the dark period (approximately 20% of baseline activity). During ad libitum feeding (wk 12 to 24), food intake, BW, body fat percentage, and total fat mass were greatly increased (P < 0.05). Compared with wk 0, circulating leptin concentrations tended to increase (P < 0.10) by wk 18 and 24 (4.45 vs. 10.02 and 9.14 ng/mL, respectively), whereas glucose (91 vs. 162 mg/dL) and triacylglyceride (30 vs. 48 mg/dL) concentrations were increased (P < 0.05) by wk 24. Adipose tissue lipoprotein lipase, hormone sensitive lipase, and adiponectin mRNA were decreased (P < 0.05) at wk 24. Adipose interleukin-6 mRNA was increased (P < 0.05) at 24 wk. Physical activity was further decreased (P < 0.05) by wk 24, during the light (60% of baseline) and dark (33% of baseline) periods. In summary, spaying and food restriction affect physical activity levels and several genes associated with lipid metabolism (decreased lipoprotein lipase), food intake (decreased leptin expression), and insulin insensitivity (increased interleukin-6). By identifying these changes, targets for nutritional intervention or lifestyle management have been identified that may curb the risk of obesity and related disorders in spayed cats.

Body lipid and fatty acid composition in male gilthead seabream broodstock at different stages of the reproductive cycle: effects of a diet lacking n-3 and n-6 HUFA

Total lipid (TL), lipid classes and fatty acid composition of neutral (NL) and polar (PL) lipids were studied in the gonads, liver and muscle of gilthead seabream males (Sparus aurata) fed a control diet (diet C) or an n-3 and n-6 highly unsaturated fatty acids (HUFA)-deficient diet (diet D), at different stages of the reproductive cycle. Between pre-spermatogenesis (November) and spermatogenesis (March), the lipid content was high and particularly rich in cholesterol, phosphatidylcholine and phosphatidylethanolamine in gonads from both dietary groups. At post-spermatogenesis (June), TL and especially PL dramatically decreased in the gonads from both groups. However, at this period diet C fish gonads were richer in triacylglycerides (TAG) than those from diet D fish. The liver lipid contents and particularly TAG were over 200% lower in June than in March for both groups. Nevertheless, the most noteworthy depletion of lipids during this period was achieved by the n-3 HUFA in diet D fish. Conversely, arachidonic acid (20:4n-6) did not decrease in NL or PL from gonads and liver in groups C and D. Muscle lipids from diet C fish were relatively insensitive to seasonal influences. However, in June, the muscle TAG content was significantly reduced in diet D fish.

Abstract 5382: Accelerated Triacylglyceride Dynamics Induced by Cardiac PPARα Overexpression are Potentiated by Adrenergic Stress to Reveal Increased Reliance on Endogenous Lipid Stores

Peroxisome proliferator-activated receptor alpha (PPARα) is a nuclear receptor involved in regulating fatty acid utilization and is implicated in the pathogenesis of diabetic cardiomyopathy. However, the involvement of PPARα in the specific utilization of endogenous lipid stores and the consequences for triacylglyceride (TAG) formation in the cardiomyocyte remain unknown. Thus, we examined TAG dynamics in isolated perfused hearts of transgenic mice exhibiting cardiac-specific over-expression of PPARα under normal and stressed conditions. Hearts from MHC-PPARα overexpressing mice (PPAR) and non-transgenic (NTG) littermates were isolated and retrogradely perfused in a 14.1 T NMR magnet with buffer containing 0.4 mM [2,4,6,8,10,12,14,16]-13C palmitate, as a source of long chain fatty acids (LCFA) + 10 mM glucose. Dynamic 13C NMR observations of the beating heart and endpoint mass spectrometry provided TAG turnover at baseline work (PPAR, n=12; NTG, n=7) and adrenergic stress (10–7 M isoproterenol, ISO) (PPAR ISO, n=5; NTG ISO, n=6). ISO elevated rate pressure product by 27% and 25% in NTG and PPAR, respectively (P&lt;0.05). At baseline workloads, PPAR contained 45% more TAG than NTG (P &lt;0.05) and TAG turnover was elevated in PPARα mice by 191% over NTG. ISO did not affect TAG content in either NTG or PPAR. However, ISO induced large increases in TAG turnover in PPAR from 1454 ± 232 to 2532 ±334 nanomoles/mg prot/min (P&lt;0.05), without affecting TAG turnover in NTG. The near doubling of TAG turnover with elevated content in MHC-PPARα hearts indicates upregulated baseline lipid metabolism via both utilization and storage pathways. Surprisingly, the adrenergic stress response potentiates the already elevated TAG dynamics in MHC-PPARα hearts, indicating persistence in matching LCFA storage rates to maintain this elevated endogenous lipid pool. Elevated TAG content in PPAR was maintained during ISO, despite increased reliance on endogenous TAG to meet the increased energy demand as compared to NTG. Therefore, activation of LCFA oxidation enzymes by PPARα is coupled in complementary fashion to the activation of LCFA storage kinetics, suggesting an expanded role for PPARα in the induction of TAG turnover.

The − 11391G/APolymorphism of the Adiponectin Gene Promoter is Associated with Metabolic Syndrome Traits and the Outcome of an Energy-restricted Diet in Obese Subjects

Adiponectin is an adipose tissue-specific hormone that is commonly decreased in obese subjects. Furthermore, single-nucleotide polymorphisms (SNPs) of the adiponectin gene have been associated with metabolic phenotypes. The present study investigated whether the adiponectin gene promoter variant -11391 G/A (rs17300539) could predict the risk of developing traits characterizing the metabolic syndrome (MetS) and the impact of weight management. The -11391 G/A SNP was genotyped in 180 Spanish volunteers (BMI: 31.4+/-3.2 kg/m (2); age: 35+/-5 years). Clinical measurements were determined at baseline, following an 8-week low-calorie diet (LCD), and at 32 and 60 weeks. At baseline, the GG genotype was associated with higher HOMA-IR, insulin and triacylglyceride concentrations than other genotypes (p<0.05) and was also related with a higher risk of insulin resistance (OR: 2.437, p=0.025) and MetS clinical manifestations (OR: 3.236, p=0.003). Following the LCD, the increased risk in GG subjects compared with others disappeared (p>0.05). By 32 weeks after dietary therapy (n=84), GG carriers had recovered the risk of metabolic comorbidities (OR: 2.420, p=0.043). This risk was even more evident after 60 weeks (OR: 2.875, p=0.014). These data show an increased risk of insulin resistance and MetS complications in obese subjects of the -11391 GG genotype. The risk was markedly reduced during an energy-restricted diet, but was not sustained. Carriage of the A allele therefore confers protection from weight regain, and the effect is particularly evident 32-60 weeks after the dietary intervention, when improvement in GG subjects had disappeared.

LET-767 Is Required for the Production of Branched Chain and Long Chain Fatty Acids in Caenorhabditis elegans

LET-767 from Caenorhabditis elegans belongs to a family of short chain dehydrogenases/reductases and is homologous to 17beta-hydroxysterol dehydrogenases of type 3 and 3-ketoacyl-CoA reductases. Worms subjected to RNA interference (RNAi) of let-767 displayed multiple growth and developmental defects in the first generation and arrested in the second generation as L1 larvae. To determine the function of LET-767 in vivo, we exploited a biochemical complementation approach, in which let-767 (RNAi)-arrested larvae were rescued by feeding with compounds isolated from wild type worms. The arrest was only rescued by the addition of triacylglycerides extracted from worms but not from various natural sources, such as animal fats and plant oils. The mass spectrometric analyses showed alterations in the fatty acid content of triacylglycerides. Essential for the rescue were odd-numbered fatty acids with monomethyl branched chains. The rescue was improved when worms were additionally supplemented with long chain even-numbered fatty acids. Remarkably, let-767 completely rescued the yeast 3-ketoacyl-CoA reductase mutant (ybr159Delta). Because worm ceramides exclusively contain a monomethyl branched chain sphingoid base, we also investigated ceramides in let-767 (RNAi). Indeed, the amount of ceramides was greatly reduced, and unusual sphingoid bases were observed. Taken together, we conclude that LET-767 is a major 3-ketoacyl-CoA reductase in C. elegans required for the bulk production of monomethyl branched and long chain fatty acids, and the developmental arrest in let-767 (RNAi) worms is caused by the deficiency of the former.

カリエスの経験に係わるヒト耳下腺液中の脂質

It has been reported that saliva may play an important role in the prevention and development of enamel caries and that both lipids and protein contents in saliva may be relevant to this role. This study examined the lipid and protein levels in saliva from individuals differing in caries experience. Female subjects (20 to 21 years old) were used divided equally into two groups, caries-susceptible group (CSG) and caries-resistant group (CRG). Stimulated parotid saliva and stimulated whole saliva were collected from the subjects. After centrifugation, each saliva sample was analyzed for the concentrations of lipids and proteins and for the compositions of lipids and fatty acids. The lipid and protein contents in parotid saliva increased in proportion to increase of the flow rate. The lipid content was slightly correlated with the protein one (r = 0.33). Total lipid and protein concentrations were higher in the samples from CSG than those from CRG. The lipid composition was similar in the samples from the two groups; more than half in total lipids was neutral lipids, followed by glycolipids and phospholipids. Neutral lipids and free fatty acid and triacylglyceride concentrations were significantly higher in the samples from CSG than those from CRG (p<0.01 for each). Also stearic, linoleic and docosahexaenoic acids were significantly higher in the former group than the latter one (p<0.05 and p<0.01). In summary, the lipid concentrations in parotid saliva from caries susceptible subjects were higher than those from caries resistant ones, and the difference in fatty acid composition was detected between them. The variations in the lipid levels and fatty acid composition may be associated with those in caries development.

Plasma concentration of leptin and ghrelin in Standardbred foals as related to the age, sex, exercise and training

The effect of acute exercise was studied in a group of 42 clinically healthy young Standardbred trotters. These trotters had been divided into four groups according to their age. Their ages were from 1.5 to 3 years. Three jugular venous blood samples were collected via venipuncture from each horse. These samples were collected while (1) at rest, (2) after the end of the exercise and (3) 30 min after the end of the exercise. Exercise showed a significant increase in plasma leptin concentration (3.8 ± 0.31 at rest v. 4.3 ± 0.37 just after exercise and 4.4 ± 0.47 ng/ml after a 30-min rest; ANOVA P < 0.05). The difference between values obtained 30 min after exercise and at rest was significantly greater in 1.5-year-old horses than in those aged 2.5 years (+1.3 ± 0.43 v. +0.1 ± 0.15 ng/ml; ANOVA P < 0.05). The mean plasma leptin concentration was higher in fillies than in colts (4.9 ± 0.47 v. 3.5 ± 0.36 ng/ml; ANOVA P < 0.05). A positive correlation between the plasma concentrations of leptin and triacylglycerides measured just after exercise was detected (r = 0.65). The acute exercise significantly increased the plasma concentration of ghrelin that was measured just after exercise (1255 ± 55.9 v. 1127 ± 54.2 pg/ml; ANOVA P < 0.05). The exercise-induced age-related changes in the plasma ghrelin concentration were significantly lower in 2.5-year-old trotters than in 1.5-year olds. To sum up, the changes in plasma leptin and ghrelin concentrations during bouts of exertion tend to decrease with age and/or training of Standardbred foals.