Healing Trend Research Articles

Micro-Electro Nanofibrous Dressings Based on PVDF-AgNPs as Wound Healing Materials to Promote Healing in Active Areas.

The purpose of this study is to develop an innovative solution for chronic wounds in high-mobility areas, such as joints, where conventional treatments are hindered by passive healing mechanisms and the need for immobilization. By designing a micro-electro-Nanofiber dressing composed of piezoelectric polyvinylidene fluoride (PVDF) integrated with antimicrobial silver nanoparticles (AgNPs), this research aims to address the dual challenges of promoting effective wound healing and maintaining joint mobility. Herein, we developed a novel micro-electro-Nanofiber dressing using electrospinning technology, incorporating polyvinylidene fluoride (PVDF) with silver nanoparticles (AgNPs). The optimized PVDF-AgNPs Nanofiber dressings exhibited strong piezoelectric effects suitable for joint wounds. In vitro experiments demonstrated that the dressing effectively promoted fibroblast migration and collagen synthesis. In vivo, the dressing exhibited a trend of rapid healing in infected wounds within 12 days while modulating macrophage differentiation toward the anti-inflammatory M2 phenotype. Additionally, the incorporation of antimicrobial nanosilver effectively controlled local infections, further facilitating the healing process. To sum up, by harnessing the piezoelectric effect to stimulate endogenous healing mechanisms without restricting joint mobility, the developed PVDF-AgNPs Nanofiber dressings represent a transformative approach for the treatment of wounds in highly mobile body areas.

Application of SVF Microinjection Combined with Skin Tension in Ischemic Chronic Refractory Wounds

ObjectiveTo compare the healing time of two groups of patients, who were randomly divided into the simple antibiotic ointment dressing change group and the simple antibiotic ointment dressing change with the skin tension group. The efficacy of SVF micro injection combined with continuous skin tension in the treatment of Ischemic CRW caused by skin ischemic necrosis after surgery was then analyzed. Regular dressing changes were performed for the patients following the above surgery in outpatient care. MethodsA retrospective cohort study was conducted from June 2022 to August 2024, including 9 patients admitted to Ruijin Hospital of Shanghai Jiao Tong University School of Medicine and 5 patients admitted to the Sixth People's Hospital affiliated with Shanghai Jiao Tong University School of Medicine. The study included a total of 14 patients (8 males and 6 females) aged between 20 and 60 years old who met the CRW criteria and had chronic refractory wounds caused by skin ischemic necrosis, with a wound area ranging from approximately 1-3cm2. Upon admission, patients with ischemic CRW underwent limited wound debridement, SVF micro injection around the wound, and PELNAC filling coverage with VSD sealing treatment. After removing the negative pressure, they were discharged and received regular dressing changes in the outpatient department. The patients were randomly divided into two groups: the antibiotic ointment dressing change group (8 cases) and the antibiotic ointment dressing change with skin tension group (6 cases). The trend of wound healing and changes in the wound were observed, while recording the wound healing time, frequency of dressing changes, and cost of dressing changes for both groups. Data from both groups were statistically analyzed using appropriate tests. ResultsThe CRW in patients significantly decreased in size and depth after surgery. Subsequently, regular dressing changes were performed in the outpatient department and the patients were randomly divided into two groups. Data analysis revealed that the healing time for wounds with skin tension during dressing changes was 24.17 ± 3.60 days, with a total of 10.67 ± 2.94 dressing changes, and a total cost of 1954.00 ± 454.79 yuan, which was significantly shorter or cheaper than those in the simple dressing change group with a healing time of 38.25 ± 5.09 days, a total of 18.25 ± 2.92 dressing changes, and a total cost of 3059.25 ±460.52 yuan; There is a significant difference, with P values <0.05. ConclusionsThe utilization of minimally invasive comprehensive surgical treatment, based on SVF microinjection therapy, has the potential to enhance the healing process of ischemic CRW. However, it has been observed that the application of antibiotic ointment for dressing changes in the later stages following the aforementioned treatments yields unsatisfactory results in terms of wound healing. Conversely, when antibiotic ointment dressing is combined with skin tension, it can expedite the healing rate of ischemic CRW and effectively ensure a positive trajectory for wound recovery. This approach not only accelerates wound healing but also demonstrates promising clinical applicability.

Self Healing Generasi Z: Bentuk Baru Perilaku Konsumtif Pada Mahasiswi FISIP ULM

The Covid-19 pandemic is the beginning of the rise of the self healing trend among generation Z when all activities must be done from home, so many people need self healing. This study aims to determine the meaning of self healing and how self healing becomes consumptive behavior. The subjects of this research were female students of the Faculty of Social and Political Sciences, Lambung Mangkurat University. This research uses a qualitative approach with phenomenological research type. Data collection techniques using observation, interviews, and documentation. The research informants amounted to 18 people, who were obtained by snowball technique. The results showed that self healing became a habit during the Covid-19 pandemic which was finally difficult to abandon and is now a new form of consumptive behavior. Generation Z (female FISIP ULM students) interpret self healing as an obligation, a burden, a way to get recognition, and show themselves on social media. Self healing is used as a sign value that is consumed to show off a lifestyle. The consumption logic of Generation Z (female FISIP ULM students) is no longer on how self healing can provide complete healing, but is replaced by a need that must always be fulfilled. This research concludes that the meaning of self healing has shifted away from its true meaning. It is important for Generation Z students and social media users to wisely filter information and trends that can trigger consumptive behavior. Self healing should be about real self-healing, not just a trend on social media that encourages someone to do it until it becomes consumptive behavior.

Investigation of Post-Transplant Mental Well-Being in Liver Transplant Recipients with Hepatic Encephalopathy.

Objective: In this study, we aimed to examine the healing trend of hepatic encephalopathy after transplantation surgery in patients with liver failure. Method: We conducted this descriptive and cross-sectional study with the participation of liver transplant recipients. A personal information form, the West Haven Criteria (WHC), the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS), and the Richmond Agitation Sedation Scale (RASS) were used for data collection. The data were analyzed using Chi-squared tests, ANOVA, and paired-samples t-tests. Results: As time progressed after liver transplantation, hepatic encephalopathy stages regressed (p < 0.01). We found that liver transplant recipients with end-stage hepatic encephalopathy were mostly within the first 6 months after transplantation, while patients with first-stage hepatic encephalopathy had received liver transplants more than 2 years ago (p < 0.01). Conclusions: The results of our study revealed that hepatic encephalopathy stages regressed after transplantation, but there was no complete recovery. This highlights the need to develop new treatment strategies other than liver transplantation for the treatment of hepatic encephalopathy.

The trend of radiographic healing after root canal treatment in teeth with apical periodontitis based on cone-beam computed tomography: A 4-year longitudinal study

ObjectivesThe aim of this study was to observe the radiographic healing of periapical lesions after root canal treatment via volumetric measurements based on cone-beam computed tomography (CBCT) over 4 years. MethodsIn total, 162 single-root teeth from patients with chronic periapical periodontitis who underwent primary root canal treatment were included in this retrospective study. Follow-up visits were scheduled at 1, 2, and 4 years after treatment. The volume of radiolucency at pretreatment and follow-up were measured, and the radiographic outcomes were classified into 4 categories: absence, reduction, uncertain or enlargement. Reduction or enlargement was considered when the volumetric change in radiolucency was 20 % or more. ResultsDuring the 4-year follow-up period, 128 teeth were reviewed at least once, including 3 extracted teeth. Of the remaining 125 teeth, the volume of radiolucency was reduced in 116 teeth (90.6 %), uncertain in 5, and enlarged in 4 teeth during 1 to 4 years after treatment. Among the 43 teeth with reduced radiolucency at 1 year after treatment, 42 (97.7 %) had continuing reduced lesions at 4 years. In the 2 teeth with enlarged radiolucency at 1 year, the volume of radiolucency doubled at 4 years. Cox regression analysis revealed that the preoperative radiolucency size was a risk factor for persistent periapical radiolucency. ConclusionsThe efficacy of root canal treatment for apical periodontitis was predictable. When the radiolucency changed by 20 % or more in volume on CBCT scans at 1 year after treatment, reversal of the radiographic healing tendency was rare. Clinical significanceThe volumetric changes in radiolucency on CBCT could reflect trends in the healing process and may foster early clinical decision-making.

Fabrication of controlled porous and ultrafast dissolution porous microneedles by organic-solvent-free ice templating method

Porous Microneedles (PMNs) have been widely used in drug delivery and medical diagnosis owing to their abundant interconnected pores. However, the mechanical strength, the use of organic solvent, and drug loading capacity have long been challenging. Herein, a novel strategy of PMNs fabrication based on the Ice Templating Method is proposed that is suitable for insoluble, soluble, and nanosystem drug loading. The preparation process simplifies the traditional microneedle preparation process with a shorter preparation time. It endows the highly tunable porous morphology, enhanced mechanical strength, and rapid dissolution performance. Micro-CT three-dimensional reconstruction was used to better quantify the internal structures of PMNs, and we further established the equivalent pore network model to statistically analyze the internal pore structure parameters of PMNs. In particular, the mechanical strength is mainly negatively correlated with the surface porosity, while the dissolution velocity is mainly positively correlated with the permeability coefficient by the correlation heatmap. The poorly water-soluble Asiatic acid was encapsulated in PMNs in nanostructured lipid carriers, showing prominent hypertrophic scar healing trends. This work offers a quick and easy way of preparation that may be used to expand PMNs function and be introduced in industrial manufacturing development.

Knowledge mapping and research trends of stem cell in wound healing: A bibliometric analysis

AbstractWound nonhealing is a common and difficult problem in clinic. Stem cells are pluripotent cells, and their undifferentiated and self‐replicating characteristics have attracted much attention in the regenerative medicine‐related researches. New treatment approaches might result from an understanding of the function of stem cells in wound healing. Using bibliometric techniques, this study proposed to analyse the research status, hotspots, and research trends in stem cell and wound healing. By using the Web of Science Core Collection (WoSCC), we conducted an in‐depth review of publications on stem cells in wound healing from 1999 to 2023. We used scientometric analysis methods to examine annual trends, institutions, countries, journals, authors, keywords, co‐occurrence references and their closed relationship, revealing present hotspots and potential future advancements in this field. We analysed 19 728 English studies and discovered a consistent rise in annual publications. The United States and China were the two countries with the most publications. The most three influential institutions in the field were Shanghai Jiao Tong University, Sun Yat‐sen University, and University of Pittsburgh. International Journal of Molecular Sciences and Biomaterials were considered the most influential journals in this field. International Journal of Molecular Sciences had the most publications, and the most quantity of citations and the highest H‐index were found in Biomaterials. The dual‐map overlay revealed that publications in Molecular/Biology/Genetics and Health/Nursing/Medicine co‐cited journals received the majority of the citations for studies from Molecular/Biology/Immunology and Medicine/Medical/Clinical. In terms of publication production and influence, Fu X stood out among the authors, and Pittenger MF took the top spot in co‐citations. According to the keywords from the analysis, future research should concentrate on the mechanisms through which stem cells promote wound healing. We conducted a thorough analysis of the general information, knowledge base and research hotspots in the field of stem cells and wound healing from 1999 to 2023 by using the VOSviewer, CiteSpace, and other bibliometric analysis tools. It not only provided valuable insights for scholars, but also served as a reliable reference that drives further development in the field and stimulates the interest of researchers.

Interaction Phenomena between Dental Implants and Bone Tissue in Case of Misfit: A Pilot Study

The biomechanical response of cortical and trabecular bone tissues represents a fundamental aspect for the interpretation of the functional response of dental implants. In the case of misfit, the interaction phenomena occurring within the surgical and the subsequent healing phases must be interpreted primarily in the light of the response of bone tissue. This is influenced by the specific loadings induced, characterized by intensity and variable trends. The pilot study reported, which intentionally refers to a simple case of a two-implants frame, is addressed to define the method to approach the biomechanical investigation of the problem and to attest the necessity to integrate clinical competences with biomechanical analysis for interpreting different aspects of osseointegration. The action induced in cortical and trabecular bone regions depending on the implant frame conformation, the surgical procedure adopted, the varying condition at the bone–implant interface and the evolutionary trend of healing are the principal aspects to be considered to evaluate the osseointegration process. The biomechanical reliability of the specific implant frame is investigated in terms of bone–implant interaction by means of numerical models. This approach can offer valid information and support clinical practice under the fundamental condition that bone biomechanical behavior is properly characterized and represented in the model, in spite of the complex formulation to be adopted.

Meta Analysis of Trends in Domestic Research on Natural Healing (2002-2022) - Focused on KCI-registered Papers -

본 연구의 목적은 국내 자연치유 연구 동향을 분석하여 자연치유의 특징들과전망을 살펴보기 위함이다. 연구를 위해 2002부터 2022년까지 KCI에 등재된 논문 중에 제목에 ‘자연치유’가 들어간 논문 52편을 선정하였다. 선정한 논문을 NCCAM의 분류체계에 따라 주제별로 분류하였다. 특히 NCCAM에 소개된 자연 치유의학 실행 요법 목록들을 세분하여 자연치유 연구 동향을 선명하게 드러내고자 하였다. 연구의 결과 게재된 논문의 제목에는 ‘자연치유’가 들어갔지만 대부분보완대체의학의 범주의 논문들이었고, 그중에 21편(40.38%)만이 NCCAM에 소개된 ‘자연치유의학(Naturopathy)’실행 요법들에 해당하는 논문이었다. 또한, 코로나를 경험하면서 자연치유 음식에 관한 인지도가 상승하였음이 확인되었다. 그뿐만 아니라 코로나 전후로 자연치유 연구가 다양한 분야와 융합되어 연구의범위가 확장되었다. 하지만 인체의 자연치유력을 높여 질병을 예방하고 치유하는 식이요법, 해독, 생활습관 등의 연구는 여전히 부족한 실정이다. 그러므로 다양한 자연적인 접근방식을 동원하는 자연요법에 관한 연구가 더 많이 진행되어야 할필요성을 제안한다.The purpose of this study is to examine the characteristics and prospects of natural healing by analyzing domestic natural healing research trends. For this study, 52 papers with ‘Natural Healing’ in the title were selected from among the papers registered in the KCI from 2002 to 2022. The selected papers were classified by topic according to the NCCAM classification system. In particular, it was intended to clearly reveal the research trend of natural healing by subdividing the list of natural healing medicine practice therapies introduced in NCCAM. As a result of the study, although ‘Natural Healing’ was included in the title of the published papers, most of them were in the category of complementary and alternative medicine. Among the research papers, only 21 out of 52 (40.38%) belonged to the catergory of naturopathy or natural remedies according to NCCAM classification. In addition, it was confirmed that the awareness of natural healing foods increased while experiencing Covid19. In addition, before and after Covid19, natural healing research has been fused with various fields, and the scope of research has been expanded. However, research efforts on diet, detoxification, and lifestyle to prevent and heal diseases by increasing the body's natural healing power is still lacking. Therefore, we suggest additional research on natural remedies and modalities.

Effects of methacrylic anhydride gelatin hydrogel loaded with silver and recombinant human basic fibroblast growth factor on deep partial-thickness burn wounds in rabbits

Effects of methacrylic anhydride gelatin hydrogel loaded with silver and recombinant human basic fibroblast growth factor on deep partial-thickness burn wounds in rabbits

Second-Intention Healing in Mohs Micrographic Surgery: A Single-Center Academic Experience.

To describe the trends in second-intention healing (SIH) use at a single Mohs micrographic surgery (MMS) dermatology clinic and assess outcomes and patient satisfaction. The authors conducted a single-center, retrospective study of patients who underwent MMS from November 2012 through November 2018. Data obtained for each patient included sex, age, tumor characteristics, number of MMS stages, final defect size, and postoperative complications. Patient satisfaction of SIH was retrospectively assessed by telephone survey. Providers used SIH in 22% of all MMS cases (n = 159/718). It was most commonly used for defects located on the nose, ear, temple, and periocular region. The average defect size and number of MMS stages for tumor clearance were 1.3 cm and 1.5 stages, respectively. Overall, low rates of postoperative complications were observed, and 95% of patients reported optimal or acceptable levels of satisfaction. This study supports the idea that SIH is a safe, effective alternative for wound management and may have broader practice indications than those traditionally proposed.

Comparison of the effectiveness of Aloe Vera gel with 2% Nitrofurazone ointment on the healing of superficial partial-thickness burns: A randomized clinical trial study

Background &amp; Aim: Routine treatment of burn injuries is dressing with Nitrofurazone ointment. It has several complications, including sensitivity. Aloe Vera is a traditional treatment for wound healing. This study aimed to compare the effectiveness of Aloe Vera gel with Nitrofurazone ointment in the healing of superficial partial-thickness burns.&#x0D; Methods &amp; Materials: The present study was a split body controlled clinical trial which was carried out on 30 patients with superficial partial-thickness burns. The study was conducted in the Shafa Hospital in Kerman, Iran, in 2016. The sample was recruited from patients who had at least two burns, each burn on an alternate side of the body. On their burns, samples were allocated to two groups that received Aloe Vera gel or 2% Nitrofurazone ointment. Bates-Jensen Wound assessment tool was used to evaluate the healing of burns. Statistical analysis was conducted by SPSS-16 and using the repeated measure ANOVA.&#x0D; Results: The repeated measure ANOVA showed that there was a significant change in the score of BWAT in all areas during the intervention period (p=0.001), but the trend of healing in the two groups during the intervention period was not significantly different (p=0.098).&#x0D; Conclusion: Based on this study, it looks like Aloe vera gel is as effective as 2% Nitrofurazone ointment in healing wounds.

Comprehensive overview on Bacillus subtilis antibacterial metabolites production

Over the last 70 years, Food processors and the plant protection sector have both benefited from Bacillus subtilis. Their capacity to manufacture endospores for survival, as well as a multitude of antimicrobial substances has piqued industrial interest in areas such as food preservation, medicinal agents, and biopesticides. In light of the growing trend of food healing and the protection of bacterial plants, this review suggests a holistic approach to visualizing the antimicrobial screen described in Group B. This review aims to make easy and updated classification of antimicrobial metabolites in group B. subtilis, its complex phylogeny that tends to perpetuate development.

Topical cannabis-based medicines - A novel adjuvant treatment for venous leg ulcers: An open-label trial.

Venous leg ulcers are highly prevalent lower limb integumentary wounds that remain challenging to heal despite the use of evidence-based compression therapies. A multitude of adjuvant treatments has been studied but none have demonstrated enough efficacy to gain adoption into treatment guidelines. Global attention on Cannabis-Based Therapies is increasing and has been driven by quantum scientific advancements in the understanding of the endocannabinoid signalling system. Topical Cannabis-Based Medicines represent a novel treatment paradigm for venous leg ulcers in terms of promoting wound closure. Fourteen complex patients with sixteen recalcitrant leg ulcers were treated with Topical Cannabis-Based Medicines in conjunction with compression bandaging, every second day, to both wound bed and peri-wound tissues. The cohort had a mean age of 75.8years and was medically complex as reflected by a mean M3 multimorbidity index score of 2.94 and a mean Palliative Performance Scale score of 67.1%. Complete wound closure, defined as being fully epithelialized, was achieved among 11 patients (79%) and 13 wounds (81%) within a median of 34days. All three remaining patients demonstrated progressive healing trends but were lost to follow-up. The treatments were well tolerated, and no significant adverse reactions were experienced. The rapid wound closure of previously non-healing venous leg ulcers among elderly and highly complex patients suggests that Topical Cannabis-Based Medicines may become effective adjuvants in conjunction with compression therapy. This may also indicate that they may have an even broader role within integumentary and wound management. Therefore, this treatment paradigm warrants being subjected to controlled trials.

Effects and mechanism of copper oxide nanozymes on wound healing of full-thickness skin defects in diabetic mice

Effects and mechanism of copper oxide nanozymes on wound healing of full-thickness skin defects in diabetic mice

Erysimum cheiri and Rosa × damascena cerate vs. Diltiazem 2% gel in the treatment of acute anal fissure: A randomized, controlled clinical trial

IntroductionAnal fissure which is an ischemic ulcer in the lining of the anus, is a common challenging problem in current medicine. This study was designed to evaluate the benefits of a Persian Medicine cerate containing wallflower (Erysimum cheiri) and damask rose (Rosa×damascena) oil (WDC) on anal fissure. MethodsThis randomized, controlled clinical trial was conducted on 64 acute anal fissure patients. In each group, patients were given either 0.5 g of WDC or 2.5 cm of Diltiazem 2% gel twice daily for 2 weeks. Anal pain, itching and inflammation were primary outcomes. The recurrence rate and statistical analyses were performed on a per protocol and intention-to-treat basis. ResultsFifty-three patients completed the study. The symptoms decreased significantly (p < 0.05) in all patients after 2 weeks. Comparison of responses between groups reveals no significant differences (p > 0.05) in pain, itching, and inflammation. There was no significant difference (p > 0.05) in recurrence rate of the disease, but the trend of healing in WDC group was better than Diltiazem 2% gel after six weeks. No serious adverse events were observed. ConclusionThe results suggest that topical application of the cerate containing wallflower and damask rose oil can be considered as a potential herbal medicine for the treatment of anal fissure. It exerts comparable clinical effects to current routine treatment. Further studies with long-term follow-ups and larger sample sizes are necessary to have a comprehensive overview of the safety and efficacy of this natural preparation.

RETRACTED ARTICLE: Novel green synthesis and antioxidant, cytotoxicity, antimicrobial, antidiabetic, anticholinergics, and wound healing properties of cobalt nanoparticles containing Ziziphora clinopodioides Lam leaves extract

The aim of the experiment was a green synthesis of cobalt nanoparticles from the aqueous extract of Ziziphora clinopodioides Lam (CoNPs) and assessment of their cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing properties. The synthesized CoNPs were characterized using different techniques including UV–Vis., FT-IR spectroscopy, X‐ray diffraction (XRD), energy dispersive X-ray spectrometry (EDS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). According to the XRD analysis, 28.19 nm was measured for the crystal size of NPs. TEM and SEM images exhibited a uniform spherical morphology and average diameters of 29.08 nm for the biosynthesized nanoparticles. Agar diffusion tests were done to determine the antibacterial and antifungal characteristics. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) were specified by macro-broth dilution assay. CoNPs indicated higher antibacterial and antifungal effects than many standard antibiotics (p ≤ 0.01). Also, CoNPs prevented the growth of all bacteria at 2–4 mg/mL concentrations and removed them at 2–8 mg/mL concentrations (p ≤ 0.01). In the case of antifungal effects of CoNPs, they inhibited the growth of all fungi at 1–4 mg/mL concentrations and destroyed them at 2–16 mg/mL concentrations (p ≤ 0.01). The synthesized CoNPs had great cell viability dose-dependently and indicated this method was nontoxic. DPPH free radical scavenging test was done to assess the antioxidant potentials, which revealed similar antioxidant potentials for CoNPs and butylated hydroxytoluene. In vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% Co(NO3)2 ointment, treatment with 0.2% Z. clinopodioides ointment, and treatment with 0.2% CoNPs ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at day 10. Use of CoNPs ointment in the treatment groups substantially raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and remarkably decreased (p ≤ 0.01) the wound area, total cells, neutrophil, and lymphocyte compared to other groups. In conclusion, CoNPs can be used as a medical supplement owing to their non-cytotoxic, antioxidant, antibacterial, antifungal, and cutaneous wound healing effects. Additionally, the novel nanoparticles (Co(NO3)2 and CoNPs) were good inhibitors of the α-glycosidase, and cholinesterase enzymes.

Biosynthesis of copper nanoparticles using Allium eriophyllum Boiss leaf aqueous extract; characterization and analysis of their antimicrobial and cutaneous wound‐healing potentials

Recently, researchers have proven the therapeutic properties of copper nanoparticles as a common product of nanotechnology science. Due to the excellent antioxidant potential of copper nanoparticles, it seems that they can be used for the treatment of infectious diseases and cutaneous wounds. Also, Allium eriophyllum Boiss leaf is used in Kurdish traditional medicine, as one of the popular medicines of the Iranian traditional medicines, for increasing the trend of wound healing. So, we decided to synthesize the copper nanoparticles containing A. eriophyllum leaf aqueous extract, and analyze their potentials in removing bacteria and fungi and healing of cutaneous wounds. After synthesizing the copper nanoparticles, they were characterized with common techniques of organic chemistry, i.e. UV–Vis, Fourier transform‐infrared, X‐ray diffraction, field emission‐scanning electron microscopy (FE‐SEM) and transmission electron microscopy (TEM). For investigating the antimicrobial properties of CuSO4, A. eriophyllum, and CuNPs@A. eriophyllum, we used the macro‐broth dilation test. Also, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of CuSO4, A. eriophyllum, and CuNPs@A. eriophyllum were gained to indicate the exact antimicrobial potentials of them. To survey the cutaneous wound‐healing effects of CuSO4, A. eriophyllum, and CuNPs@A. eriophyllum, the 0.2% ointments were formulated and compared with the control and untreated groups. All data of antimicrobial and cutaneous wound‐healing experiments were assessed by SPSS 22 software. The results of chemical characterization indicated that copper nanoparticles had been synthesized as the best form, and the size ranges were 25–35 and 30–35 nm in the FE‐SEM and TEM images, respectively. In the antimicrobial part of this study, CuNPs@A. eriophyllum indicated higher antifungal and antibacterial effects than all standard antibiotics (P ≤ 0.01). MIC, MBC and MFC of CuNPs@A. eriophyllum were 2−4 mg/ml against all fungi and bacteria tested. With regard to cutaneous wound healing, the use of CuNPs@A. eriophyllum ointment significantly (P ≤ 0.01) raised the wound contracture, vessel, hydroxyl proline, hexuronic acid, hexosamine, fibrocyte and fibrocytes/fibroblast rate, and significantly (P ≤ 0.01) decreased the wound area, total cells, neutrophils and lymphocytes compared with other groups in rats. According to the above findings, CuNPs@A. eriophyllum may be used for the treatment of infectious diseases and cutaneous wounds in humans.

Autologous Platelet-Rich Plasma in the Treatment of Perianal Fistula in Crohn’s Disease

AimTo assess clinical healing in patients with perianal Crohn’s disease with local intrafistular injection of autologous platelet-rich plasma. MethodThe pilot study was conducted at a single centre between January 2013 and December 2015. Autologous platelet-rich plasma was prepared in platelet-rich and platelet-poor fractions for local intrafistular injection in patients with proven, established perianal Crohn’s disease. Patients were permitted biological therapies, and the Perianal Crohn’s Disease Activity Index was recorded. Patients were followed for 48 weeks for clinical signs of healing (complete, partial or non-healing), monitoring fistula drainage, closure and epithelialization. ResultsThe study included 29 patients (19 males; mean age 38 ± 12.8 years) with four exclusions in the operating room because surgery was not indicated and four lost to follow-up. Five adverse events were recorded, with two requiring the drainage of abscess collections. Of the 21 patients assessable at 24 weeks, there was complete healing, partial healing and non-healing in 7 (33.3%), 8 (38.1%) and 6 (28.6%) patients, respectively. By 48 weeks, there was complete healing, partial healing and non-healing in 6 (40%), 6 (40%) and 3 (20%) patients, respectively, with a reduction in the number of visible external fistula openings at both time points (P = 0.021). By the end of the study, there was a higher trend of healing if biological therapies were continued (85.7% with biologics vs. 75% without, P = 0.527), but there were no statistically significant differences and no differences in the Perianal Crohn’s Disease Activity Index. ConclusionAutologous platelet-rich plasma is safe in patients with perianal Crohn’s disease, with an acceptable healing rate over a medium-term follow-up, particularly if biological therapies are used concomitantly.

Therapeutic effects of Glycyrrhiza glabra aqueous extract ointment on cutaneous wound healing in Sprague Dawley male rats

Glycyrrhiza glabra has been reported to have wide applications in the treatment of many human diseases. However, its traditional use in the treatment of wounds has not been validated by any scientific study. Also, its safety in the management of cutaneous wound conditions requires attention. The purpose of the experiment was to investigate the wound healing potential of G. glabra aqueous extract ointment. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous wound, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, treatment with 3% G. glabra aqueous extract ointment (3 g of G. glabra aqueous extract + 97-g base ointment). At days 10, 20, and 30 after creating the wound, for histopathological and biochemical analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. The data were analyzed using one-way ANOVA followed by Duncan post hoc test of SPSS-22 software. At days 10, 20, and 30, G. glabra aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the wound area, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of wound contracture, fibrocyte, hexuronic acid, and hydroxyproline as compared with the basal ointment and control groups. In conclusion, the present study has demonstrated that the aqueous extracts of G. glabra have properties that render them capable of promoting accelerated wound-healing activity compared with other groups.