Purpose: Using Landsat OLI remote sensing images in the two phases of 2015 and 2020. Taking Nanjing as the research area. Based on RS and GIS technology, adopting maximum likelihood method for supervised classification, extracting and producing a land use classification map for the two phases of Nanjing from 2015 to 2020. And further, using the land use change transfer matrix to analyze and summarize the characteristics and laws of the dynamic changes of land use in Nanjing. Result: From 2015 to 2020 in Nanjing, the overall land use change showed “two reductions and four increases”. The area of arable land and bare land decreased by 729.46 km2, 332.31 km2; the area of u rban construction land, woodland, grassland, and water area increased by 448.39 km2, 231.67 km2, 216.87 km2 and 164.84 km2. And each land use type has a different amount of area conversion. Conclusion: The analysis of land use change based on Landsat remote sensing image research area can effectively predict the change trend of regional land use. It can provide a basis for the research on the driving forces of land use change in the region, and it can also provide decision-making support for the formulation of land-related policies in the region.