Tree Uptake Research Articles

Linking Mineral Nutrition and Fruit Quality to Growth Intensity and Crop Load in Apple

Diagnostic of the nutritional status in fruit trees can be estimated using different models. However, these models usually do not fully match the character of the perennial crops and their accuracy needs to be improved. This study aimed at deepening the understanding of the aspects controlling mineral content of macro- and micronutrients and fruit quality of the apple trees with respect to the crop load and vegetative shoot growth. The focus was given on description of the nutrient content variability in time and space, its relation to important physiological events, and conditions altering their gradient in leaf and fruit. The evaluation was carried out with six years old ’Golden Delicious’ apple trees on M9, planted in an experimental orchard. Flowering rate, total yield, shoot growth intensity, mean fruit weight and quality along with mineral content of leaves, and fruit in different development stages were analysed according to intensity of natural or regulated flowering and fruit set, respectively. We found that aside of macroelements, iron, and manganese depended on the crop load. For nitrogen, phosphorus, and potassium content, their gradient increased up to 10–25% within the sufficiency range as the trees tried to maintain certain level of these nutrients in leaves and fruit simultaneously during deficit. In contrary, the higher content of Mg, Fe, and Mn related to the avoidance of deficit through up to 20–30% higher uptake in trees with higher crop load. Phosphorus, potassium, and calcium content in short fruiting shoots was more stage dependent, showing relation with the timing of cessation of particular meristematic growth activity. If no intense deficit occurred, the trees kept unrestricted initial investment of nutrients in the fruit during cell division stage whatever the crop load was. Their content at the later development stages appeared to be more dependent upon the consumption by particular sink. These findings allowed to improve the interpretation of the fruit trees nutrition status due to adaptation of the reference nutrient content in low cropping trees by a reduction of 5–10% N and P, 10–15% Ca, as well as by an increase of 15–20% K content in leaves at the stage with fruit size of 20 mm, and without change in P content at the stage of about 70% of final fruit size.

Role of photosynthesis and stomatal conductance on the long-term rising of intrinsic water use efficiency in dominant trees in three old-growth forests in Bosnia-Herzegovina and Montenegro

Old-growth forests have an important role in maintaining animal and plant diversity, are important carbon (C) reservoirs and are privileged sites to study long-term plant physiological responses, long-term forest dynamics and climate change impact on forest ecosystems. Several studies have highlighted how old-living trees undergo age-related declines with hydraulic limitations and reduction in photosynthesis, though some recent works have suggested that such a decline is not always observed. Our study aims at understanding the role of atmospheric CO2 increase on tree C uptake and stomatal conductance (gs) in old-living trees by analysing the long-term patterns of tree growth and intrinsic water use efficiency (iWUE) in three old-growth forests in the Balkans (Bosnia-Herzegovina and Montenegro), using dendrochronology and isotopic analysis. We hypothesised a long-term increase in iWUE in the studied old-growth forests, mostly related to enhanced photosynthesis rather than reduced stomatal conductance. Tree cores were sampled from dominant silver fir (Abies alba Mill.) trees in each forest. Tree-ring widths were measured and basal area increments (BAI) were assessed for each sampled tree and, from the six longest chronologies, five decades were chosen for cellulose extraction, its isotopic analysis (δ13C, δ18O), iWUE and leaf water 18O evaporative enrichment above the source water (Δ18OL) determination. We observed a continuous and significant increase in iWUE from 1800 to 2010 in the sampled dominant trees at all the three old-growth forests. Our BAI data and our estimates of Δ18OL across the study period support the idea that enhanced photosynthesis rather than reduced stomatal conductance is the major driver of the measured iWUE increase. Thus, our results support some recent findings challenging the hypothesis that iWUE in forests is primarily the result of a CO2-induced reduction in stomatal conductance as well as the so called hydraulic limitation hypothesis.

A single-layer urban canopy model with transmissive radiation exchange between trees and street canyons

Urban trees are one of the most effective strategies to mitigate excessive heat stress in cities. To understand the underlying mechanisms of their cooling effect and to assess their use in urban planning, the accurate simulation of how trees interact with the ambient built environment is critical and imperative. However, the representation of urban trees in existing urban canopy models (in particular the single-layer ones) remains oversimplified. Here we develop a new Monte Carlo ray tracing method to explicitly resolve the canopy transmittance and evaluate its impact on radiative view factors between trees and regular building facets. The new method is highly accurate in reproducing analytical solutions. Sensitivity tests of radiative view factors suggest the importance of canopy transmittance in changing the radiation exchange. We then incorporate the ray tracing algorithm into the new version of the Arizona State University (ASU) Single-Layer Urban Canopy Model (ASLUM v3.1). In addition to radiation transmittance, ASLUM v3.1 explicitly resolves the radiative shading, evapotranspiration, and root water uptake of urban trees in street canyons, with significantly improved performance in predictions (especially latent heat flux) when compared to previous versions. We further apply ASLUM v3.1 to evaluate the impacts of trees with varying characteristics on urban radiation exchange and turbulent heat fluxes. Results show that urban trees reduce the net radiation of ground and wall as well as the daytime temperature via shading and transpiration, but may slightly warm the nighttime street canyons through radiative trapping effect.

Dynamics of changes in the concentrations of herbicides and nutrients in the soils of a combined wheat-poplar tree cultivation: a field experimental model during the growing season

The efficiency of a wheat-poplar agroforestry system was investigated in order to assess nutrient and pesticide uptake in an experimental plot located in Athens, Greece, during the spring–summer cultivating period of 2017. Soil sampling was performed on a monthly basis and the monitored pollutants were NO3−–N, NO2−–N, NH4+–N and PO43−–P ions, and the herbicides pendimethalin along with its metabolite M455H001, iodosulfuron-methyl-sodium and mesosulfuron-methyl-sodium. Soil analysis was performed using Liquid Chromatography tandem mass spectrometry and Ion Chromatography. The tested tree-crop system exhibited reductions of more than 80% and up to 100% for nutrients, whilst with regards to the examined herbicides and M455H001 metabolite, their lessening was more than 75% and up to 100% in the closest to the tree row points compared to the control point away from the trees. In conclusion, agroforestry alley cropping systems exhibit a potential for tree uptake of excess pesticides and nutrients and may act as a technique for pollution abatement, since tree roots that extend below the crops can uptake the excess of agrochemicals that would otherwise remain in soil or enter ground and surface waters.

Seasonal Fe Uptake of Young Citrus Trees and Its Contribution to the Development of New Organs.

This work quantifies Fe uptake in young citrus trees, its partitioning among plant compartments, and the contribution of the Fe absorbed from fertilizer to the development of new tissues. A soil pot experiment was conducted using 4-year-old clementine trees (Citrus clementina Hort ex Tan), and a dose of 240 mg Fe was applied by labeled fertilizer (92% atom 57Fe excess). Plants were uprooted at five different phenologic states: end of flowering (May 15), end of fruit setting and fruit drop (July 1), two fruit growing moments (August 1 and October 15), and at complete fruit maturity (December 10). The Fe accumulated in the root system exceeded 90% of the total Fe content in the plant. All organs progressively enriched with 57Fe (8.5–15.5% and 7.4–9.9% for young and old organs, respectively). Reproductive ones reached the highest increase (111% between May and October). 57Fe enrichment from woody organs reflects an increasing gradient to sink organs. The root system accumulated 80% of the Fe absorbed from the fertilizer, but the young organs accumulated relatively more Fe uptake during flowering and fruit setting (15.6% and 13.8%, respectively) than old organs (around 9.8%). Although iron derived from fertilizer (Fedff) preferably supplied young organs (16.7–31.0%) against old ones (2.5–14.9%), it only represented between 13.8% and 21.4% of its content. The use efficiency of the applied Fe (FeUE) barely exceeded 15%. The lowest FeUE were found in young and old organs of the aerial part (1.1–1.8% and 0.7–1.2%, respectively). Since the pattern of the seasonal absorption of Fe is similar to the monthly distribution curve of the supplied Fe, it is recommended that the application of Fe chelates in calcareous soils should be performed in a similar way to that proposed in this curve.

Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach.

Ammonium (NH4+) plays key roles in plant growth, development, fruit quality, and yield. In plants, NH4+ uptake and transport are facilitated by NH4+ transporters (AMT). However, molecular mechanisms and physiological functions of type-II AMT (AMT2) transporters in fruit trees are still unclear, especially in peach. In this study, we cloned and characterized an AMT2 family gene from peach, PpeAMT3;4, and determined its function in yeast mutant. Expression analysis showed that PpeAMT3;4 was majorly expressed in peach roots and significantly decreased by NH4+ excess but had no response to NH4+ deficiency. Functional determination and 15nitrogen-labeled NH4+ uptake assay in yeast cells implied that PpeAMT3;4 was a typical high-affinity transporter, with a Km value of 86.3 μM, that can uptake external NH4+ in yeast cells. This study provides gene resources to uncover the biological function of AMT2 transporters and reveals molecular basis for NH4+ uptake and nitrogen (N) nutrition mechanisms in fruit trees.

Water uptake plasticity of savanna trees in encroached grassland: small trees match the mature trees

Processes linked to woody plant encroachment in grassland are still not well understood, especially the interactions between trees and soil water availability. Our aim was to study the depth of water uptake by trees in grassland and its controlling factors. We studied water uptake in Vachellia sieberiana trees (hereafter called ‘acacias’) in a humid grassland of South Africa. We especially studied the effect of size classes of acacias and their position in the catena on water potential (ψ) of acacia leaves and water uptake depth of acacias across seasons, as inferred from isotope δ18O values of soil and stem water samples. Acacias were more water stressed in February (wet season) with the most negative water potentials. Taller acacias (>3 m height) were more water-stressed (ψ ± SD = −1.62 ± 0.81 MPA) than smaller acacias (<1 m height) (ψ ± SD = −1.32 ± 0.80 MPA). In September (end of dry season), all tree size classes uptake water equally in A and B soil horizons. In February (middle of the wet season), tall, medium and even small trees favoured the B horizon. Small trees had similar plasticity in water uptake to taller trees and could modify depth of water uptake between seasons. This process could increase their probability of becoming adults, and may favour tree encroachment in grasslands.

A δ2H offset correction method for quantifying root water uptake of riparian trees

Root water uptake plays an important role in water cycle in Groundwater-Soil-Plant-Atmosphere-Continuum. Stable isotopes (δ2H and δ18O) are effective tools to quantify the use of different water sources by plant roots. However, the widespread δ2H offsets of stem water from its potential sources due to δ2H fractionation during root water uptake result in conflicting interpretations of water utilization using stable isotopes. In this study, a potential water source line (PWL), i.e., a linear regression line between δ18O and δ2H data of both soil water at different depths and groundwater, was proposed to correct δ2H offsets of stem water. The PWL-corrected δ2H was determined by subtracting the deviation between δ2H in stem water and PWL from the original value. The MixSIAR model coupled with seven types of input data (i.e. various combinations of single or dual isotopes with uncorrected or corrected δ2H data by PWL or soil water line (SWL)) were used to determine seasonal variations in water uptake patterns of riparian tree of Salix babylonica (L.) along the Jian and Chaobai River in Beijing, China. These methods were evaluated via three criteria including Akaike Information Criterion (AIC), Bayesian Information Criterion (BIC) and root mean square error (RMSE). Results showed that different types of input data led to considerable differences in the contributions of soil water at upper 30 cm (9.9–57.6%) and below 80 cm depths (29.0–76.4%). Seasonal water uptake patterns were significantly different especially when δ2H offset was pronounced (p < 0.05). The dual-isotopes method with uncorrected δ2H underestimated the contributions of soil water in the 0–30 cm layer (by 30.4%) and groundwater (by 56.3%) compared to that with PWL-corrected δ2H. The PWL correction method obtained a higher groundwater contribution (mean of 29.5%) than that estimated by the SWL correction method (mean of 24.5%). The MixSIAR model using dual-isotopes with PWL-corrected δ2H produced the smallest AIC (94.1), BIC (91.9) and RMSE values (4.8%) than other methods (94.9–101.7, 92.6–99.5 and 5.3–12.4%, respectively), which underlined the best performance of PWL correction method. The present study provides crucial insights into quantifying accurate root water uptake sources even if δ2H offset exists.

Investigation of pollution history in XKS mining area in China using dendrochronology and LA-ICP-MS.

Past pollution episodes and the intensity of mining activity in a contaminated region can be unraveled, using archived elemental information in tree rings. Elemental dendrochronology can be utilized with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to make a timeline of metal(loid)s uptake in trees. In this study, six tree core samples from five tree species were collected around the XKS antimony mine region in Hunan, China, which is impacted by metal pollution: NM1 shuitong (Camptotheca acuminate L.), M1 shuitong (Camptotheca acuminate L.), G1 xiangchun (Toona sinensis L.), J2 wutong (Firmiana platanifolia L.), BSN kulian (Melia azedarach L.), and MT1 zhang (Cinnamomum camphora L.). Tree cores were dated by counting annual rings, and concentrations of As, Ca, Pb, Sb, and Zn were determined using LA-ICP-MS. Results demonstrate that the highest concentrations of Sb are present in the areas closer to mining activity: NM1 - north mine (shuitong), MT1 - mine tailing site (zhang), and BSN - between south and north mine (kulian) tree cores have average Sb concentrations of 18.8mg/kg, 13mg/kg, and 4.8mg/kg, respectively. In comparison, at the village sites located farther away from the mining areas, G1 xiangchun, J2 wutong, and M1 shuitong have average Sb concentrations of 0.69mg/kg, 0.57mg/kg, and 0.66mg/kg, respectively. NM1 shuitong, G1 xiangchun, and MT1 zhang all have large Sb and Zn peaks around 1986, while BSN kulian has larger Sb peaks slightly later around 1988-1990. J2 wutong has Sb peaks somewhat earlier, at 1977 and 1980. Unlike the others trees, M1 shuitong has greater Sb concentrations in more recent years (2009, 2015-2016) demonstrating Sb pollution.

Effects of drought on nitrogen uptake and carbon dynamics in trees.

Research on drought impact on tree functioning is focussed primarily on water and carbon (C) dynamics. Changes in nutrient uptake might also affect tree performance under drought and there is a need to explore underlying mechanisms. We investigated effects of drought on (a) in situ nitrogen (N) uptake, accounting for both, N availability to fine roots in soil and actual N uptake, (b) physiological N uptake capacity of roots and (c) the availability of new assimilates to fine roots influencing the N uptake capacity using 15N and 13C labelling. We assessed saplings of six different tree species (Acer pseudoplatanus L., Fagus sylvatica L., Quercus petraea (Mattuschka) Liebl., Abies alba Mill., Picea abies (L.) H.Karst. and Pinus sylvestris L.). Drought resulted in significant reduction of in situ soil N uptake in deciduous trees accompanied by reduced C allocation to roots and by a reduction in root biomass available for N uptake. Although physiological root N uptake capacity was not affected by drought in deciduous saplings, reduced maximum ammonium but not nitrate uptake was observed for A. alba and P. abies. Our results indicate that drought has species-specific effects on N uptake. Even water limitations of only 5weeks as assessed here can decrease whole-plant inorganic N uptake, independent of whether the physiological N uptake capacity is affected or not.

Nutrient Uptake in Huanglongbing-affected Sweet Orange: Transcriptomic and Physiological Analysis

Since the advent of Huanglongbing [HLB (Candidatus Liberibacter asiaticus)] in Florida, several preliminary reports have emerged about the positive effects of mineral nutrition on the performance of HLB-affected citrus (Citrus sp.) trees. HLB-affected trees are known to undergo significant feeder root loss. Therefore, studies have focused on foliar nutrient application instead of soil-applied nutrients speculating that the HLB-affected trees root systems may not be competent in nutrient uptake. Some studies also suggest that HLB-affected trees benefit from micronutrients at higher than the recommended rates; however, the results are often inconclusive and inconsistent. To address this, the goal of the present study was to evaluate the nutrient uptake efficiency and the quantitative and qualitative differences in nutrient uptake of HLB-affected trees. HLB-affected and healthy sweet orange (Citrus sinensis) trees were grown in a 100% hydroponic system with Hoagland solution for 8 weeks. The trees were deprived of any fertilization for 6 months before the transfer of trees to the hydroponic solution. Altogether, the four treatments studied in the hydroponic system were healthy trees fertilized (HLY-F) and not fertilized (HLY-NF), and HLB-affected trees fertilized (HLB-F) and not fertilized (HLB-NF). HLY-F and HLY-NF trees were found to have similar levels of leaf nutrients except for N, which was found to be low in nonfertilized trees (HLY and HLB). Both HLB-F and HLB-NF trees had lower levels of Ca, Mg, and S compared with HLY trees. In addition, HLB-NF trees had significantly lower levels of micronutrients Mn, Zn, and Fe, compared with HLY-NF trees. The hydroponic solution analysis showed that HLB-F and HLY-F trees had similar uptake of all the nutrients. Considering that HLB-affected trees have a lower root-to-shoot ratio than healthy trees, nutrient uptake efficiency per kilogram of root tissue was significantly higher in HLB trees compared with HLY trees. Under nutrient-deficient conditions (day 0) only nine genes were differentially expressed in HLB roots compared with HLY roots. On the other hand, when fertilizer was supplied for ≈1 week, ≈2300 genes were differentially expressed in HLB-F roots compared with HLY-F roots. A large number of differentially expressed genes in HLB-F were related to ion transport, root growth and development, anatomic changes, cell death, and apoptosis compared with HLY-F trees. Overall, anatomic and transcriptomic analyses revealed that HLB-affected roots undergo remarkable changes on transitioning from no nutrients to a nutrient solution, possibly facilitating a high uptake of nutrients. Our results suggest the roots of HLB-affected trees are highly efficient in nutrient uptake; however, a small root mass is a major limitation in nutrient uptake. Certain micronutrients and secondary macronutrients are also metabolized (possibly involved in tree defense or oxidative stress response) at a higher rate in HLB-affected trees than healthy trees. Therefore, a constant supply of fertilizer at a slightly higher rate than what is recommended for micronutrients and secondary macronutrients would be beneficial for managing HLB-affected trees.

A single tree model to consistently simulate cooling, shading, and pollution uptake of urban trees

Extremely high temperatures, which negatively affect the human health and plant performances, are becoming more frequent in cities. Urban green infrastructure, particularly trees, can mitigate this issue through cooling due to transpiration, and shading. Temperature regulation by trees depends on feedbacks among the climate, water supply, and plant physiology. However, in contrast to forest or general ecosystem models, most current urban tree models still lack basic processes, such as the consideration of soil water limitation, or have not been evaluated sufficiently. In this study, we present a new model that couples the soil water balance with energy calculations to assess the physiological responses and microclimate effects of a common urban street-tree species (Tilia cordata Mill.) on temperature regulation. We contrast two urban sites in Munich, Germany, with different degree of surface sealing at which microclimate and transpiration had been measured. Simulations indicate that differences in wind speed and soil water supply can be made responsible for the differences in transpiration. Nevertheless, the calculation of the overall energy balance showed that the shading effect, which depends on the leaf area index and canopy cover, contributes the most to the temperature reduction at midday. Finally, we demonstrate that the consideration of soil water availability for stomatal conductance has realistic impacts on the calculation of gaseous pollutant uptake (e.g., ozone). In conclusion, the presented model has demonstrated its ability to quantify two major ecosystem services (temperature mitigation and air pollution removal) consistently in dependence on meteorological and site conditions.

Cadmium Uptake in Native Cacao Trees in Agricultural Lands of Bagua, Peru

Cadmium (Cd) contamination threatens cocoa farming in the province of Bagua in Amazonas, Peru. This study reports our assessment of Cd concentrations in cocoa farm soils, and in cocoa roots, leaves, testa, and cotyledon, thus evaluating the magnitude of the problem caused by Cd exposure. For our analysis, we sampled agricultural soil, cocoa roots, leaves and pods at 29 farms in the province of Bagua. Concentrations of Cd in each of the samples were measured and correlated with selected variables at each sampling site. Within our collection of samples, Cd levels showed great variability. In soil, Cd concentrations ranged between 1.02 and 3.54 mg kg−1. Concentrations of this metal within cocoa trees measured from roots, leaves, testa, and cotyledon, Cd ranged from 0.49 mg kg−1 to 2.53 mg kg−1. The cocoa trees exhibited variable degrees of allocation Cd from the soil to their tissues and thus considerable variation among themselves. We found that Cd amounts in roots were up to five times more concentrated than Cd levels in the soils and 2.85 times [Cd] the amounts found in cotyledon. Soil pH is a key variable enabling the uptake of this metal. Most importantly, our evaluation determined that measurements from the majority of farms exceeded the maximum permissible limits established by Peruvian and European legislation.

Temporal offset between precipitation and water uptake of Mediterranean pine trees varies with elevation and season

For climate models that use paleo-environment data to predict future climate change, tree-ring isotope variations are one important archive for the reconstruction of paleo-hydrological conditions. Due to the rather complicated pathway of water, starting from precipitation until its uptake by trees and the final incorporation of its components into tree-ring cellulose, a closer inspection of seasonal variations of tree water uptake is important. In this study, branch and needle samples of two pine species (Pinus pinaster and Pinus nigra subsp. laricio) and several water compartments (precipitation, creek, soil) were sampled over a two-year period and analyzed for the temporal variations of their oxygen and hydrogen stable isotope ratios (δ18O and δ2H) at five sites over an elevation gradient from sea level to around 1600 m a.s.l. on the Mediterranean island of Corsica (France). A new model was established to disentangle temporal relationships of source water uptake of trees. It uses a calculation method that incorporates the two processes mostly expected to affect source water composition: mixing of waters and evaporation. The model results showed that the temporal offset from precipitation to water uptake is not constant and varies with elevation and season. Overall, seasonal source water origin was shown to be dominated by precipitation from autumn and spring. While autumn precipitation was a more important water source for trees growing at mid- (~800–1000 m a.s.l) and high-elevation (~1600 m a.s.l.) sites, trees at coastal sites mostly took up water from late winter and spring. These findings show that predicted decreases in precipitation amounts during the wet season in the Mediterranean can have strong impacts on water availability for pine trees, especially at higher elevations.

Seasonal variations in photosynthetic functions of the urban landscape tree species Gingko biloba: photoperiod is a key trait

Seasonal variations in the leaf photosynthetic traits of an urban tree, Ginkgo biloba, were almost synchronized with the photoperiod. Non-stomatal limitations were a cue for photosynthesis in Ginkgo biloba. Photosynthetic functions, which are key traits in determining the carbon uptake of urban trees, exhibit significant seasonal variations in temperate zones. It is essential to clarify the seasonal dynamics of photosynthesis to evaluate the CO2 uptake in urban areas. We investigated seasonal variations in the photosynthetic traits of Ginkgo biloba, which is a major urban deciduous tall tree often planted in Japan. Seasonal variations in the leaf photosynthetic traits, including the maximum photosynthesis rate, maximum carboxylation rate, and mesophyll and stomatal conductance, were well fitted to quadratic models, in which they peaked around the summer solstice and then declined with time. Seasonal variations in the environmental variables, such as photoperiod, temperature, and solar radiation, were compared to those of the leaf photosynthetic traits, in which the photoperiod explained well variations in the leaf photosynthetic traits. Seasonal variations in photosynthesis were largely governed by non-stomatal limitations, i.e., mesophyll and biochemical limitations. The high synchrony of the photoperiod and photosynthetic traits during leaf maturation may cause an enhancement in the daily carbon uptake of G. biloba leaves around the summer solstice, which has the longest photoperiod, and thus, will lead to an increase in the annual carbon uptake.

Fourteen years of compost application in a commercial nectarine orchard: effect on microelements and potential harmful elements in soil and plants

The objective of this experiment was to valuate, after 14 years, the impact of annual compost applications on micronutrient and potentially toxic trace elements on nectarine tree uptake and soil fertility. The study was performed in the Po valley, Italy, on the variety Stark RedGold (grafted on GF677). Since orchard planting, the following treatments were applied, in a randomized complete block design, with four replicates: 1. unfertilized control; 2. mineral fertilization (N was supplied as NO3NH4 at 70–130 kg ha−1 year−1); 3. compost at 5 t DW ha−1 year−1; 4. compost at 10 t DW ha−1 year−1. The actual rate of application was 12.5 (LOW) and 25 (HIGH) t ha−1, since compost was concentrated in the tree row. Compost was made from domestic organic wastes mixed with pruning material from urban ornamental trees and garden management and stabilized for 3 months.The supply of compost HIGH induced an enrichment of soil total Cu, Zn and Cd, and a decrease of Fe and Co concentration; with values always below the European threshold limits for heavy metals in the soil. In addition, compost (at both rates) increased availability (DTPA-extractable) of Fe, Mn and Zn, Cd, Ni, and Pb in the top soil (0–0.15 m).Total micronutrient and trace element tree content was not affected by fertilization treatments; however, the recycled fraction returned to the soil at the end of the season through abscised leaves and pruned wood of Cu, Fe, Mn and Zn was increased by mineral fertilization; Fe and Zn also by compost HIGH. Our data show that the introduction of compost at both 12.5 and 25 t ha−1 year−1 in the row did not increase the risk of pollution related to potentially toxic trace elements and at the same time increased the bioavailability of Fe, Mn and Zn.

Does ASCAT observe the spring reactivation in temperate deciduous broadleaf forests?

Scatterometer observations over land are sensitive to the water content in soil and vegetation, but have been rarely used to study seasonal changes in the plant water status and seasonal development of deciduous trees. Here we use Advanced Scatterometer (ASCAT) observations to investigate the sensitivity of C-band backscatter to spring phenology of temperate deciduous broadleaf forests in Austria. ASCAT's multi-angle looking capability enables the observation of backscatter over a large range of incidence angles. The vegetation status affects the slope of the backscatter-incidence angle relationship. We discovered a maximum in the slope around the month April, hereafter referred to as spring peak, predominantly in regions covered by deciduous broadleaf forest. We hypothesized that the spring peak indicates the average timing of leaf emergence in the deciduous trees in the sensor footprint. The hypothesis was tested by comparing the timing of the spring peak to leaf unfolding observations from the PEP725 phenology database, to the increase of leaf area index (LAI) during spring, and to temperature. Our results demonstrate a good agreement between the ASCAT spring peaks, phenology observations and temperature conditions. The steepest increase in LAI however lags behind the ASCAT peak by several days to a few weeks, suggesting that the spring peak in fact marks the timing of maximum woody water content, which occurs right before leaf emergence. Based on these observations, we conclude that the ASCAT signal has a high sensitivity to spring reactivation and in particular water uptake of bare deciduous broadleaf trees. Our findings might provide the basis for novel developments to estimate eco-physiological changes of forests during spring at large scales.

Approaches to Understand Historical Changes of Mercury in Tree Rings of Japanese Cypress in Industrial Areas

Historical changes of mercury (Hg) concentrations in tree rings of Japanese cypress (Chamaecyparis obtusa) and the role of dendrochemistry over the last 50 years in Yeosu and Gwangyang National Industrial Complexes of South Korea were evaluated. Mercury uptake in trees were found to be bidirectional and variable depending on atmospheric Hg conditions. With low atmospheric Hg concentrations, Hg concentrations in tree rings were significantly influenced by soil Hg concentrations via roots. With high atmospheric Hg concentrations, Hg concentrations in tree rings were dominated by atmospheric Hg uptake via foliage. Patterns of Hg concentration in sampling sites were divided into: (1) a linear increase in low concentration of Hg originated from soils via roots during 1967–1977 and (2) an elevated and constant concentration with spatial variation of Hg concentration due to foliar uptake from atmosphere during 1978–2014. Between 1967 and 1977, when shrubs and vegetation senesced each year, there was an annual source of Hg in soils due to continued deposition of Hg to soil via litterfall and debris. Thus, Hg concentration was increased over time. During these periods, Hg concentrations in tree rings reflected uptakes of Hg through roots under young forest and low atmosphere Hg conditions. Whether tree rings can serve as reliable proxies for atmosphere Hg concentrations remain unclear due to Hg uptakes from soils and limits from atmosphere under low atmospheric Hg conditions. Intensified chemical plants and steel mills have continued throughout Yeosu and Gwangyang industrial areas since late 1970s, resulting in high Hg emissions. Hg concentrations in tree rings during 1978–2014 showed elevated and constant levels. In addition, tree ring Hg concentrations at study sites were increased gradually with decreasing distance from industrial areas, with a high concentration of 11.15 ng/g at the Yeosu site located the nearest to industrial areas and a low concentration of 4.34 ng/g at the Suncheon site which was the farthest away from industrial areas.

The expanding role of deep roots during long‐term terrestrial ecosystem development

AbstractModels of ecosystem development and response to environmental variation must incorporate change in vertical soil space as well as over time. Insufficient measurement of subsurface soil properties represents a major observational bias in ecosystem studies.We address these changes in horizontal (time) and vertical (soil profile) space along a three‐million‐year, semi‐arid, piñon‐juniper woodland substrate age gradient with characteristic progressive and retrogressive ecosystem development phases and a shift from nitrogen (N) and water to phosphorus (P) limitation. We present a novel pedological approach using isotopic tracers and biogeochemical analyses to address fine root distribution, depth of plant uptake and relative nutrient availabilities.We show that (a) the quantity of fine roots remains constant with ecosystem development but their distribution in the soil profile becomes increasingly deeper and less concentrated in the surface soil; (b) mean depth of tree uptake becomes deeper with substrate age and follows the relative availability of P as P‐limitation develops and (c) soil P transformations in the oldest soil profiles resemble the theoretical changes with age to produce a depth gradient of relative N and P availability.Synthesis. The expanding role of deep roots in this model system is tightly linked to phases of ecosystem development and relative nutrient availability. The inclusion of whole soil profiles is vital to investigating the intersections of biota, soil and geologic substrate and developing a more complete understanding of ecosystem structure and function.

Iodine uptake and translocation in apple trees grown under protected cultivation

AbstractBackground and Aims: Agronomic biofortification of food crops with iodine may improve the dietary intake of this trace element, which is essential for human development and health. So far, little is known about the suitability of this technique in pome fruits. The objectives of this study were (1) to investigate uptake and translocation of exogenously applied iodine in apple trees, (2) to identify possible strategies of iodine biofortification for this type of fruit, and (3) to evaluate interactions between foliar applied iodine and selenium.Methods: Apple trees were cultivated in a plastic tunnel for two growing seasons. Iodine was applied via leaves or substrate. During the 2nd year, simultaneous foliar application of iodine and selenium were tested as well. At harvest time, iodine and selenium content in leaves and fruits were determined. The phytoavailable iodine concentration in the growing medium was analyzed following an extraction with calcium chloride. In addition, the dynamics of iodine applied as potassium iodide and iodate in a peat‐based substrate was investigated in an incubation experiment without plants.Results: The iodine concentration in washed apples increased more than 100‐fold, valuing around 50 µg (100 g FM)−1 by foliar application of iodine as compared to the control treatment. However, this level was only achieved in fruits which were directly wetted by the spray solution. The translocation of leaf‐absorbed iodine to fruits was negligible. Following a substrate fertilization, the fruit iodine content remained rather low due to a strong retention of iodine in the growing medium. When using foliar sprays, the addition of selenium did not affect the iodine enrichment of the apple fruits.Conclusions: Foliar fertilization of iodine seems to be a promising method to biofortify apples with iodine. The level of I achieved in apple fruits by means of foliar fertilization can significantly contribute to the daily I intake requirement of humans.