Tree-ring Variability Research Articles

Neighborhood competition modulates the link between crown structure and tree ring variability in monospecific and mixed forest stands

3D crown shape and tree ring development are autobiographies of the growth conditions. With advancements in terrestrial laser scanning, modeling growth from 3D crown structure offers insights into trees’ structural and functional responses in a non-destructive and repetitive way. However, how the trees in different growing conditions respond in 3D structure and manifest their responses into tree rings remains unexplored, which would help to better explain tree-level growth dynamics and forest management. To enhance this understanding, we tested a set of hypotheses: (HI) that 3D crown shape (CS) and tree ring (width) patterns (TRP) are correlated across different growing conditions or forest stands like monospecific, provenances trials, and mixed forest stands; (HII) that stand types influence the CS-TRP link; and (HⅢ) local neighborhood competition (LNC) modulates the CS-TRP link. (HⅣ) 3D crowns manifest local growth conditions; therefore, 3D crown structures can be used to predict tree ring growth. We assessed these hypotheses by employing terrestrial laser scanning-based 3D crown shape and dendrochronology-based tree ring width patterns from Norway spruce (Picea abies [L.] Karst.) trees growing in pure spruce (unthinned and thinned), provenances trial, and mixed-species trial (with European Beech, Fagus sylvatica [L.]) stands covering a large-scale competition gradient. We first show that 3D crown shape and TRP metrics differed significantly across forests (p<0.05) but were correlated (p<0.05). Neighborhood competition among the forest types influences the link between 3D crowns and tree rings. Pathway-based analyses revealed that neighborhood competition indirectly influences ring variability by modifying crown structure (p<0.05), suggesting local growth conditions are mostly manifested into crown shapes, leading to 3D crown shape-based low-error growth predictions (0.44 mm) across forest types. However, incorporating competition legacy information (competition over the last 30 years) in the model slightly improved the prediction performance (error reduced to 0.41 mm), further explaining that the crown likely loses growth information due to growing conditions (competition it faces and species with it is growing). This study reveals how trees in different growing conditions differed structurally and mechanized their responses in tree rings, providing crucial insights into tree-level growth dynamics and management.

Warming effects on tree-ring variables in P. hartwegii Lindl. at the extremes of its natural elevational distribution in central Mexico

In alpine forests, the impact of warming differs at the upper and lower extremes of the elevation gradient causing trees to compensate through different adaptive mechanisms. The objective was to retrospectively evaluate the radial growth response to recent interannual climate variation in Pinus hartwegii trees growing at the extremes of their elevational gradient on two volcanic mountains in central Mexico (La Malinche and Nevado de Toluca). Increment cores collected from 174 P. hartwegii trees were used to estimate time-series of growth ring including ring width and density variables, as well as basal area increment (BAI) and ring biomass (BIO) for 1964–2016. Differences in average radial growth and time-trends of ring variables in response to climate variables were estimated based on correlations of ring variables, BAI, and BIO with inter-annual variation in temperature, rainfall, and length of the frost-free period (FFP). Results showed higher average values of ring width and density variables, as well as BAI and BIO (i.e., higher productivity) at low elevation on both mountains. Time-trends for BAI and BIO were positive at both ends of the elevation gradient, but ring width and density variables followed opposite trends. Inter-annual variation of ring variables was explained more by temperature than by rainfall or FFP; however, temperature was negatively correlated with RW and positively with RD, even though BAI and BIO were positively correlated with temperature (All them with P < 0.001) at both elevational end on both mountains. For most of the ring variables, the total variation explained by the ANOVA model was more related to the linear warming trend (greater than 67%). Warming is affecting radial growth of P. hartwegii trees differently at high versus low elevation on both mountains, showing warning signs from the perspective of functional traits, but not in terms of productivity.

Disentangling the Evidence of Milankovitch Forcing From Tree-Ring and Sedimentary Records

Tree-ring records constitute excellent high-resolution data and provide valuable information for climate science and paleoclimatology. Tree-ring reconstructions of past temperature variations agree to show evidence for annual-to-centennial anomalies in past climate and place the industrial-era warming in the context of the late Holocene climate patterns and regimes. Despite their wide use in paleoclimate research, however, tree rings have also been deemed unsuitable as low-frequency indicators of past climate. The arising debate concerns whether the millennia-long tree-ring records show signals of orbital forcing due to the Milankovitch cycles. Here, we produce a summer-temperature reconstruction from tree-ring chronology running through mid- and late-Holocene times (since 5486 BCE) comprising minimum blue channel light intensity (BI). The BI reconstruction correlates with existing and new tree-ring chronologies built from maximum latewood density (MXD) and, unlike the MXD data, shows temperature trends on Milankovitch scales comparable to various types of sedimentary proxy across the circumpolar Arctic. Our results demonstrate an unrevealed potential of novel, unconventional tree-ring variables to contribute to geoscience and climate research by their capability to provide paleoclimate estimates from inter-annual scales up to those relevant to orbital forcing.

Global Ecopotential of Dendrochronology

Drought is a meteorological phenomenon that has influenced the life of human society for ages and still has a great impact on it. This phenomenon can be divided into a group of sub-phenomena, e.g. soil and air droughts, winter droughts, etc.… From a physiological point of view, drought may have different consequences in different periods of a growing season. It seems however difficult to describe quantitative characteristics of a physiologically important drought using any meteorological parameters because within an ecosystem the results of the interaction of ecological factors may change the way plants respond to a certain limited number of values of meteorological parameters. Photosynthesis and transpiration characteristics predetermine that the function modelling annual tree ring variability will always and under any conditions have a variable that will depend on drought. The objective of dendroclimatic studies can be either to separate a drought-associated climatic signal from the tree ring chronology or, on the contrary, to search a chronology with a “clear” and easily detected drought-associated signal. Having retrospective aspects, Dendroclimatic studies show considerable promise for the evaluation of droughts including those that have an impact on crop yields. Such studies can give further information for crop yield forecasts.

Comparing automatically generated and manually measured tree-ring transects of growth trends with Hawaiian sandalwood as an example species

Tree-ring measurements are a primary quantitative tool used in numerous scientific disciplines. Some species, however, exhibit morphological complexities leading to significant uncertainty in these measurements. Hawaiian Sandalwood (Santalum paniculatum) stems, for example, often develop asymmetric growth features that hinder tree-ring measurements. These features include faint-ring boundaries and wedging rings which disappear in portions of the cross-section. In this work we a use a novel two-dimensional transect methodology and our own open-source software, svg-dendro, to analyze particularly difficult cross-sections. Our method accomplishes this by first tracing all rings by hand and automatically generating a user-specified number of transects. On average, these traced measurements had more sensitivity to tree-ring variability without losing important equivalencies with the traditional binocular stereomicroscope technique (e.g., radii, skewness) as indicated by greater mean variance for ring number, mean tree-ring width, and standard deviation. All S. paniculatum samples had ring wedging, where certain sides of the stem had many locally absent tree rings but to different intensities. The new technique allows us to analyze the shift from complete rings with little to no wedging to rings with more wedging starting between the 19th and 40th ring, where deep stem lobes begin forming. The new method also reveals the difficulty in measuring these trees, as the wedging creates multiple lobes with different visible ring counts. This research suggests that this two-dimensional methodology would be best applied to non-circular trees with fewer incomplete rings, supporting the importance of species and population selection. Overall, we have developed an efficient and flexible means to measure otherwise unmeasurable growth features in tree samples through representing tree-ring boundaries as curves and developing software to sort and map transects.

Phenotypic plasticity of European larch radial growth and wood density along a-1,000 m elevational gradient.

Phenotypic plasticity is a key mechanism for sedentary long-living species to adjust to changing environment. Here, we use mature Larix decidua tree-ring variables collected along an elevational transect in the French Alps to characterize the range of individual plastic responses to temperature. Stem cores from 821 mature Larix decidua trees have been collected from four plots distributed along a 1,000-m elevational gradient in a natural forest to build up individual linear reaction norms of tree-ring microdensity traits to temperature. The sign, magnitude and spread of variations of the slopes of the individual reaction norms were used to characterize variation of phenotypic plasticity among plots and traits. Results showed a large range of phenotypic plasticity (with positive and negative slopes) at each elevational plot and for each tree-ring variable. Overall, phenotypic plasticity tends to be larger but positive at higher elevation, negative at the warmer lower sites, and more variable in the center of the elevation distribution. Individual inter-ring reaction norm is a valuable tool to retrospectively characterize phenotypic plasticity of mature forest trees. This approach applied to Larix decidua tree-ring micro-density traits along an elevation gradient showed the existence of large inter-individual variations that could support local adaptation to a fast-changing climate.

Alpine Holocene tree-ring dataset: age-related trends in the stable isotopes of cellulose show species-specific patterns

Abstract. Stable isotopes in tree-ring cellulose are important tools for climatic reconstructions even though their interpretation could be challenging due to nonclimate signals, primarily those related to tree aging. Previous studies on the presence of tree-age-related trends during juvenile as well as adult growth phases in δD, δ18O, and δ13C time series yielded variable results that are not coherent among different plant species. We analyzed possible trends in the extracted cellulose of tree rings of 85 larch trees and 119 cembran pine trees, i.e., in samples of one deciduous and one evergreen conifer species collected at the tree line in the Alps, covering nearly the whole Holocene. The age trend analyses of all tree-ring variables were conducted on the basis of mean curves established by averaging the cambial-age-aligned tree series. For cambial ages over 100 years, our results prove the absence of any age-related effect in the δD, δ18O, and δ13C time series for both the evergreen and the deciduous conifer species, with the only exception being larch δD. However, for lower cambial ages, we found trends that differ for each isotope and species; i.e., mean δ13C values in larch do not vary with aging and can be used without detrending, whereas those in cembran pine show a juvenile effect, and the data should be detrended. Mean δ18O values present two distinct aging phases for both species, complicating detrending. Similarly, mean δD values in larch change in the first 50 years, whereas cembran pine changes between 50 and 100 years. Values for these two periods of cambial age for δD and δ18O should be used with caution for climatic reconstructions, ideally complemented by additional information regarding mechanisms for these trends.

Multi‐variable approach pinpoints origin of oak wood with higher precision

AbstractAimSpatial variations of environmental conditions translate into biogeographical patterns of tree growth. This fact is used to identify the origin of timber by means of dendroprovenancing. Yet, dendroprovenancing attempts are commonly only based on ring‐width measurements, and largely neglect additional tree–ring variables. We explore the potential of using wood anatomy as a dendroprovenancing tool, and investigate whether it increases the precision of identifying the origin of oak wood. Since different tree–ring variables hold different information on environmental conditions prevailing at specific times of the growing season—which vary between source regions—we hypothesize that their inclusion allows more precise dendroprovenancing.LocationEurope, Spain.TaxonQuercus robur L., Quercus petraea (Matt.) Liebl., Quercus faginea Lam., Quercus pyrenaica Willd.MethodsWe sampled four oak species across Northern Spain, i.e. from the Basque country and Cantabria and—in the Basque country—from low to high elevation (topographic/latitudinal gradient). We measured multiple tree–ring variables to (a) extract complementary variables; (b) present statistical relations among them; (c) analyse region‐specific variation in their patterns based on time–series of individual trees; and (d) determine underlying climate–growth relationships. Leave‐one‐out analysis was used to test whether a combination of selected variables allowed dendroprovenancing of a randomly selected tree within the area.ResultsA combination of latewood width (LW) and earlywood vessel size was used to pinpoint the origin of oak wood with higher precision than ring width or LW only. Variation in LW pinpointed the wood to east and west areas, whereas variation in vessels assigned wood to locations along a latitudinal/topographic gradient. The climatic triggers behind these gradients are respectively an east–west gradient in June–July temperature and a north–south gradient in winter/spring temperatures. The leave‐one‐out analyses supported the robustness of these results.Main conclusionsIntegration of multiple wood–xylem anatomical variables analysed with multivariate techniques leads to higher precision in the dendroprovenancing of ring‐porous oak species.

Climate forcing of tree growth in dry Afromontane forest fragments of Northern Ethiopia: evidence from multi-species responses

BackgroundClimate-induced challenge remains a growing concern in the dry tropics, threatening carbon sink potential of tropical dry forests. Hence, understanding their responses to the changing climate is of high priority to facilitate sustainable management of the remnant dry forests. In this study, we examined the long-term climate-growth relations of main tree species in the remnant dry Afromontane forests in northern Ethiopia. The aim of this study was to assess the dendrochronological potential of selected dry Afromontane tree species and to study the influence of climatic variables (temperature and rainfall) on radial growth. It was hypothesized that there are potential tree species with discernible annual growth rings owing to the uni-modality of rainfall in the region. Ring width measurements were based on increment core samples and stem discs collected from a total of 106 trees belonging to three tree species (Juniperus procera, Olea europaea subsp. cuspidate and Podocarpus falcatus). The collected samples were prepared, crossdated, and analyzed using standard dendrochronological methods. The formation of annual growth rings of the study species was verified based on successful crossdatability and by correlating tree-ring widths with rainfall.ResultsThe results showed that all the sampled tree species form distinct growth boundaries though differences in the distinctiveness were observed among the species. Positive and significant correlations were found between the tree-ring widths and rainfall, implying that rainfall plays a vital role in determining tree growth in the region. The study confirmed the formation of annual growth rings through successful crossdating, thus highlighted the potential applicability of dendroclimatic studies in the region.ConclusionsOverall, the results proved the strong linkage between tree-ring chronologies and climate variability in the study region, which further strengthens the potential of dendrochronological studies developing in Ethiopia, and also has great implications for further paleo-climatic reconstructions and in the restoration of degraded lands. Further knowledge on the growth characteristics of tree species from the region is required to improve the network of tree-ring data and quality of the chronology so as to successfully reconstruct historic environmental changes.

From xylogenesis to tree rings: wood traits to investigate tree response to environmental changes

ABSTRACTIt is noteworthy that the largest part of global vegetation biomass depends on a thin layer of cells: the vascular cambium. Understanding the wood formation processes and relationships with environmental factors is a crucial and timely research question requiring interdisciplinary efforts, also to upscale the information gained and to evaluate implications for tree growth and forest productivity.We provide an overview of wood formation processes up to tree-ring development, bearing in mind that the combined action of intrinsic factors and environmental drivers determines the anatomical traits of a tree ring formed at a specific time and position within the tree’s architecture. After briefly reviewing intrinsic factors, we focus attention on environmental drivers highlighting how a correct interpretation of environmental signals in tree rings must be grounded in a deep knowledge of xylogenesis and consequent wood anatomical traits. We provide guidelines on novel methods and approaches recently developed to study xylem formation. We refer to existing literature on established techniques for retrospective analyses in tree-ring series of anatomical and isotopic traits, to assess long-term ecophysiological responses to environmental variations, also giving advice on possible bias because of interand within-tree variability.Finally, we highlight that, once the temporal axis of intra-annual tree-ring variability of xylem traits is established by xylogenesis analysis, a multidisciplinary approach linking classical dendro-ecology, wood functional traits (dendro-anatomy) and eco-physiology (here focusing on dendro-isotopes) allows a better interpretation of past environmental events hidden in tree rings, and more reliable forecasts of wood growth in response to climate change.

Wood anatomical traits highlight complex temperature influence on Pinus cembra at high elevation in the Eastern Alps.

Climate sensitivity of populations at the margins of their distribution range is of key importance to understand species' responses to future warming conditions. Pinus cembra is of particular interest being a typical high-elevation taxon, spread with mostly scattered populations within its actual range, but still overlooked in traditional dendrochronological researches due to low tree-ring variability and climate sensitivity. With a different approach, we analyzed time series of xylem anatomical traits, split into intra-ring sectors, and used daily climate records over 89years (1926-2014) aiming to improve the quality and time resolution of the climate/growth associations. From nine trees growing at their altitudinal limit and on 1.5 × 106 tracheids, we measured ring width (MRW), cell number per ring, lumen area (LA), and cell-wall thickness (CWT). We then computed correlations with monthly and fortnightly climate data. Late-spring and summer temperature emerged as the most important factors. LA and especially CWT showed a stronger temperature response than MRW, starting in mid-May and early June, respectively. CWT also evidenced the longest period of correlations with temperature and a significant difference between latewood radial and tangential walls. Analysis of xylem anatomical traits at intra-ring level and the use of daily temperature records proved to be useful for high resolution and detailed climate/growth association inferences in Pinus cembra.

Gene expression profiling of periodontitis-affected gingival tissue by spatial transcriptomics

This study represents the first large-scale systematic dendroclimatic sampling focused on developing chronologies from different species in the eastern Mediterranean region. Six reconstructions were developed from chronologies ranging in length from 115years to 600years. The first reconstruction (18852000) was derived from principal components (PCs) of 36 combined chronologies. The remaining five, 18002000, 17002000, 16002000, 15002000 and 14002000 were developed from PCs of 32, 18, 14, 9, and 7 chronologies, respectively. Calibration and verification statistics for the period 19312000 show good levels of skill for all reconstructions. The longest period of consecutive dry years, defined as those with less than 90% of the mean of the observed MayAugust precipitation, was 5years (15911595) and occurred only once during the last 600years. The longest reconstructed wet period was 5years (16011605 and 17511755). No long term trends were found in MayAugust precipitation during the last few centuries. Regression maps are used to identify the influence of large-scale atmospheric circulation on regional precipitation. In general, tree-ring indices are influenced by MayAugust precipitation, which is driven by anomalous below (above) normal pressure at all atmospheric levels and by convection (subsidence) and small pressure gradients at sea level. These atmospheric conditions also control the anomaly surface air temperature distribution which indicates below (above) normal values in the southern regions and warmer (cooler) conditions north of around 40N. A compositing technique is used to extract information on large-scale climate signals from extreme wet and dry summers for the second half of the twentieth century and an independent reconstruction over the last 237years. Similar main modes of atmospheric patterns and surface air temperature distribution related to extreme dry and wet summers were identified both for the most recent 50years and the last 237years. Except for the last few decades, running correlation analyses between the major European-scale circulation patterns and eastern Mediteranean summer precipitation over the last 237years are non-stationary and insignificant, suggesting that local and/or sub-regional geographic factors and processes are important influences on tree-ring variability over the last few centuries.

The influence of increasing temperature and CO2 concentration on recent growth of old-growth larch: contrasting responses at leaf and stem processes derived from tree-ring width and stable isotopes.

Time series of tree-ring growth show significant increases since the early 1970s at the alpine tree line, with simultaneously increasing temperatures and atmospheric CO2 concentration. For a comprehensive understanding of this growth change, the physiological response patterns at both the leaf and stem level need to be separately analyzed and identified, and can be retrieved from tree-ring growth and isotope (δ13C, δ18O) series. In this study, we assessed the relative contribution of environmental factors to interannual tree-ring variability by multivariate linear mixed-effects models and the dual isotope approach on a dataset of tree-ring records of ~400-year-old larch (Larix decidua Mill.) from a non-water-limited high-elevation site in the Swiss Alps. The models suggest that summer temperatures and the recent lack of larch budmoth outbreaks were most important for explaining growth variations and trends, while a significant direct effect of the continuously increasing CO2 concentration could not be confirmed. In contrast, δ13C and δ18O, which are strongly influenced by fractionation changes in the leaf, clearly reflected the impact of air humidity (precipitation and vapor pressure deficit) and CO2 concentration: the increase in (δ13C-derived) intrinsic water-use efficiency over the second half of the 20th century suggests an increase in carbon assimilation as a result of enhanced CO2 concentration. The tree-ring δ18O largely reflected recent precipitation as source water, thus indicating a low variability in stomatal conductance, which was confirmed by the dual isotope approach. These leaf-level effects were not reflected in stem growth as they may have been masked by the temperature-caused growth limitation controlling the allocation of increased amounts of photosynthates into wood growth. Our approach demonstrates that the identification of different roles of environmental factors on leaf and stem processes helps to improve the assessment of site-specific changes of carbon fluxes and growth performance under future environmental conditions.

Climate of the late Pleistocene and early Holocene in coastal South China inferred from submerged wood samples

Tree-ring variability of submerged wood specimens in coastal areas provides important clues about sea level change and climate variability of the past. We dated submerged wood samples from coastal Fujian province in China using the radiocarbon methods and investigated their tree-ring variability. The submerged wood samples from the Shenhu Bay that date to the early Holocene (∼8000 calibrated years B.P.) may be related to the rise of the sea level after the Last Glacial Maximum (LGM). The submerged wood samples from the Qianhu Bay site dated to the Marine Isotope Stage 3 (MIS 3) (>40,000 calibrated years B.P.). Most of the submerged wood samples are from coniferous trees with frequent branching tree rings. Frequent branching tree rings in this region are mostly found from the currently endangered coniferous species with narrow ecological amplitude at humid sites. Tree rings of the submerged sample show conspicuous interdecadal variability (∼20 years) than interannual variations, which differs from modern tree rings of nearby regions which have stronger interannual and multi-decadal variability. Our study highlights the potential to use submerged samples of coastal Southeast China for paleoclimate studies.

Growth responses to climate in a tree-ring network of European beech (Fagus sylvatica L.) from the eastern limit of its natural distribution area

The first dendrochronological network of European beech (Fagus sylvatica L.) from eastern limit of its distribution was established covering the species’ altitudinal range (210–1160m a.s.l.) and three eco-regions. Statistical analysis revealed spatial trends among the 14 chronologies, with increasing tree-ring variability and synchronicity towards the east, particularly expressed by rBAR (mean interseries correlation). By means of HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analysis), the chronologies were divided into three main groups: a Mountain, Plateau and Eastern class. These correspond to the Alpine South, Continental and Pannonian bioclimatological zones. Dendroclimatological analysis revealed a general climate response pattern, with summer temperatures, April–May precipitation and April–June drought being the main limiting factors in the study area. The intensity of the correlations increases towards the eastern distribution limits and decreases towards the upper treeline of European beech. Similarly, the length of the climate-sensitive period changes, with the precipitation window increasing from April in the Mountains region to March–May in the East region and the scPDSI (self-calibrated Palmer drought severity index) window increasing from April–June in the Mountains to the previous July to the current September in the East. The western and uppermost site shows signs of being limited by low July temperatures. This chronology network represents a sensitive monitoring instrument to quantify the impact and adaptability of European beech to actual and future climatic change.

Sex-related climate sensitivity of Araucaria araucana Patagonian forest-steppe ecotone

Dioecious species offers the possibility to discern plant development in relation to sex. However, there is little information of the incidence of sex on tree-growth sensitivity to seasonal variations in climate. In this study we aim to examine the relationship between the climate and tree growth variability from Araucaria araucana inferred from independent male and female tree-ring chronologies derived from old growth forests growing on the xeric environments of NW Patagonia in Argentina. The analysis included 186 standing trees (93 males and 93 females) distributed in five forest stands. After applying a Generalized Linear Mixed Models (GLMMs), result suggests significant differences between tree-ring width related to both sex and climatic conditions. When considering the relationships between tree growth and climate, sensitivity of tree-ring variability to climate appeared to be sex-dependent. While male trees are more sensitive to land precipitation during part of the current growing period, female trees appears to be more sensitive to air surface temperature during the prior period of growth. At atmospheric circulation scales, male trees are more sensitive to the Southern Annular Mode index during the current period of growth. The reported differences in the sensitivity of the response to climate from both male and female trees can be attributed to different mechanisms in the use of the environmental resources. In relation to recent trends in climate experienced in northern Patagonia, these changes, if continued, may compromise the growth and reproductive fitness of the A. araucana forests.

Spatiotemporal alignment of radial tracheid diameter profiles of submontane Norway spruce

Studying intra-annual wood formation dynamics provides valuable information on how tree growth and forests are affected by environmental changes and climatic extreme events. This study has the aim to evaluate and to quantify synergetic potentials emerging from a combination of current state of the art techniques used to monitor intra-annual wood formation processes. Norway spruce trees were studied in detail during the growing season 2009 with weekly sampling of microcores, high resolution point-dendrometers and wood anatomical analysis. The combination of the applied techniques allowed us to convert the spatial scales of radial tracheid diameter profiles to seasonal time scales and to synchronize fluctuations in intra-annual cell diameter profiles. This spatiotemporal information was used to validate the recently introduced software MICA (Multiple interval-based curve alignment). In comparison to the conventional approach of averaging profiles of tree ring variables, the MICA aligned profiles exhibit a significantly higher synchronicity of the averaged data points. We also demonstrate two new features in the MICA application that enable to extrapolate spatiotemporal information between intra-annual profiles for the construction of robust mean (consensus) profiles that are representative for the population dynamics. By using a set of complementary techniques in an integrated approach, this study highlights a new methodological framework that can contribute to a better understanding of the environmental control of wood formation during the growing season.

Tree ring variability and climate response of Abies spectabilis along an elevation gradient in Mustang, Nepal

In mountainous areas including the Himalayas, tree lines are expected to advance to higher altitudes due to global climate change affecting the distribution and growth of plant species. This study aimed at identifying the tree ring variability of Abies spectabilis (D. Don) and its response to the climate along an elevation gradient in the high Himalayas of central Nepal. Tree core samples were collected from four sites in Mustang district. All sites were located in the same valley and exposed to similar weather conditions. Out of 232 samples collected from the sites, Titi lower (2700 m), Titi upper (2900 m), Pangukhark (3100 m) and Lete upper (3300 m), 44, 40, 39 and 41 series were successfully cross-dated and ring-width chronologies including 168, 79, 138 and 156 years previous to 2012 were developed, respectively. Statistically significant differences in average annual radial growth were noted among the four sites with the highest radial growth observed at mid-elevation sites. Chronological statistics based on residual chronologies for the common period revealed that A. spectabilis at the upper elevation site was more climate sensitive than at the other three sites. At the highest-elevation sites the correlation between pre-monsoon precipitation and tree growth was positive, and for the month of May this was statistically significant (p&lt;0.05). Moreover, spring temperature (March-June) was negatively correlated with precipitation and with tree growth at all sites, and at the upper elevation site (3300 m) the correlation was significant for March, April and May. Banko Janakari, Vol. 24, No. 1 pp. 3-13

Early summer temperatures reconstructed from black pine (Pinus nigra Arnold) tree-ring widths from Albania

The first regional chronology of black pine ( Pinus nigra Arnold) from Albania, its response to climate and a 428-year long reconstruction of June–July temperatures for the 1583–2010 period are presented. Samples were collected at five locations from the north to the south of Albania. We constructed a 552-year long Pinus nigra chronology with sufficient sample depth for potential climate reconstruction from 1583 to 2010 (428 years). Response to precipitation was significant only for July of the current year (0.23), while a clear temperature response to the May–August period was identified, with July having by far the highest correlation (−0.47). When combined, June–July temperatures had the highest correlation of all tested combinations ( r = −0.63), explaining almost 40% of the tree-ring width variability. The test of the climate signal temporal stability using a 31-year running correlation highlighted the very stable relationship between tree-ring indices and June–July temperatures. The spatial extent of the regional Pinus nigra chronology tested, using a field correlation function, showed very high spatial correlation over a large part of the Balkan Peninsula and even southern Italy. Based on high explained variance between tree-ring indices and June–July temperatures and significant values of reduction of error (RE) and coefficient of efficiency statistics (CE; 0.62 and 0.25 in the calibration period), a linear model was developed, with June–July temperatures reconstructed for the 1583–2010 period. Our reconstruction and identified extreme years were compared with various temperature, drought and precipitation reconstructions from nearby regions, as well as data from documentary archives. We confirmed a high degree of similarity of our reconstruction with other reconstructions and documentary data.

A cluster of stratospheric volcanic eruptions in the AD 530s recorded in Siberian tree rings

Recently published, improved chronologies for volcanic sulfate in Greenland and Antarctic ice permit a comparison of the growth responses of absolutely annually dated tree rings at three locations in Siberia with annual ice-core records of volcanic eruptions centered on AD 536. For the first time for this region and period, we present unique data sets for tree-ring width, cell-wall thickness, δ13C and δ18O in cellulose. These were based on multiple samples from relict wood of larch obtained from two sites close to the northern limit of tree growth on the Taimyr Peninsula and in northeastern Yakutia, and at a high-elevation, location 20° further South in the Altai Mts. An event in AD 536 was associated with different, but marked, changes in tree-ring parameters at the high-latitude sites compared with the high elevation site. An AD 541 event was associated with its own distinctive tree-ring responses across the three sites and multiple variables. The years after AD 532 were marked by a strong and sustained decrease in growth at the high-elevation, more southerly, site. The combination of improved ice-core chronology for the climatically effective volcanic eruptions of this part of the 6th century AD, and an array of tree-ring sites with different climates and multiple tree-ring variables permits a richer description of tree responses to this cluster of events. The pattern of tree-ring parameter responses at the three locations in AD 536, AD 541, and perhaps AD 532 is consistent with responses to climatically effective volcanic eruptions influencing tree response in those and subsequent years.