P1 Treatment Research Articles

Effects of curing method on quality of vanilla pod using a greenhouse effect dryer

The problem faced in Indonesian vanilla production and export is the high variation of vanilla quality. This is caused by some factors, including variations in the age of harvest, curing process and drying method. This study aimed to determine the effects of curing using a greenhouse effect (GHE) dryer on the quality of vanilla pods. The vanilla pod was harvested and subjected to the withering process by soaking in water at a temperature of 60-65°C for 2 mins. After that, the sweating process was carried out for 2 days at room temperature. Then, the samples were subjected to four curing treatments [drying with GHE dryer with no sweating process (P1), drying with GHE dryer combined with sweating in the first two days (P2), drying with GHE dryer combined with sweating in the first four days (P3) and drying with sun drying combined with sweating in the first two days (P4)]. Drying was continued for all treatments until the water content of the vanilla pod reached 34-35%. The changes in color, shape, weight loss, water and vanilla content of vanilla pod before and after the curing process were observed. The results showed that drying with a GHE dryer combined with sweating in the first four days (P3) significantly affects the vanillin content and aroma of cured vanilla pod. There was no effect of curing on the color of cured vanilla pods. The P3 treatment was the best curing method since the vanillin content was highest with acceptable color and aroma.

Study on the Mechanism of Nd:YAG Laser-Assisted Therapy on the Changes of Subgingival Flora in Periodontitis.

This study evaluated the therapeutic efficacy of combining Neodymium-doped Yttrium Aluminum Garnet (Nd:YAG) laser with subgingival curettage and root planing (SRP) in generalised stage III/grade C periodontitis patients and its effects on cytokine dynamics and microbial community. Fifteen patients diagnosed with stage III/grade C periodontitis were included in the cohort. The right and left sides of the mouth were randomly assigned either the conventional SRP (control) group or the SRP supplemented with Nd:YAG laser group (experimental group, 160 mJ, 4 W) in a split-mouth design. Clinical periodontal indices were recorded at baseline and at the 6-week follow-up post-treatment. ELISA was utilised to measure IL-1β and TNF-α levels in gingival crevicular fluid. The subgingival microbiota's composition and variations were characterised using 16S rDNA amplicon sequencing, while quantitative real-time polymerase chain reaction (qRT-PCR) was employed to analyse the changes in the red-complex bacteria in subgingival plaque. The SRP+Nd group exhibited a statistically significant reduction in record probing depth (PD) and bleeding on probing (BOP) compared to the SRP group after treatment (p0.05). The SRP+Nd group showed a markedly lower IL-1β level than the SRP group (p0.05). Furthermore, there was no statistically significant difference in the dominant subgingival microbiota composition and level of the red-complex bacteria between the two groups (p>0.05). The adjunctive use of Nd:YAG laser with SRP demonstrates promising short-term therapeutic benefits for patients with extensive stage III/grade C periodontitis. Both SRP as a standalone treatment and its combination with Nd:YAG laser effectively facilitate a transition in the dominant bacterial community from periodontitis-associated to periodontal health-associated microbiota.

Sweet corn (Zea mays L.) seed performance enhanced under drought stress by chitosan and minerals coating.

Sweet corn (Zea mays L.) var. Saccharata is a tropical and semitropical annual cereal with low germination, poor vigor, and weak seedling establishment in the soil. In order to enhance the physical properties of sweet corn and examine the effects of seed coating on the morphological, biochemical, and physiological characteristics of sweet corn seedlings under drought stress conditions, we conducted a factorial experiment in greenhouse conditions. Seed coating was carried out using a mixture of vermiculite (V), kaolin (K), and perlite (P) in a ratio of 3:1.5:2. The main factors of the greenhouse experiment comprised three levels of coating treatment (chitosan 0.5% + V10K2.5P5 (gr), NaAlg 1% + V10K2.5P5 (gr), and non-coated seeds as a control) along with drought stress at four levels (0, -0.3, -0.6, and -0.9bar). In greenhouse conditions, the growth indexes of sweet corn seedlings were studied under increasing levels of drought stress. The results showed that as drought stress levels increased, certain growth indicators such as seedling emergence and seedling emergence rate, soluble protein, chlorophyll total content, nitrogen, and phosphorus content decreased. On the other hand, mean emergence, proline, potassium, soluble sugars, malondialdehyde, and hydrogen peroxide were increased. The study found that the highest seedling emergence percentage occurred in the coating treatment of chitosan 0.5% + V10K2.5P5 (gr) at all levels of drought stress. Overall, seed coating with the Chitosan 0.5% + V10K2.5P5 (gr) treatment improved the performance of sweet corn seeds and reduced the negative effects of drought stress by increasing seedling emergence and establishment.

Feed palatability and consumption of Eclectus Roratus Biaki in The Captivity of Bird and Ochid Park in Biak Numfor

This study aimed to assess the palatability and feed consumption of bayan parrots (Eclectus roratus biaki) in the captivity at the Bird and Orchid Park in Biak, using a variety of local feed combinations. The feeds consisted of fruits (papaya and bananas) and seeds (mung beans and sweet corn). A total of 8 parrots (4 males and four females) were used, housed in pairs within individual cages. Four treatments were tested: P0 (papaya and banana), P1 (papaya, banana, and mung beans), P2 (papaya, banana, and sweet corn), and P3 (papaya, banana, mung beans, and sweet corn). Feed and water were provided ad libitum and feed consumption was measured by weighing the remaining feed daily. The results indicated that bananas had the highest palatability and consumption across all treatments, with the highest feed consumption occurring in the P3 treatment, which included a combination of fruits and seeds. Palatability index, dry matter intake, and organic matter intake were higher when parrots were offered a varied diet. No significant differences were found between treatments in daily consumption, but the variety in feed combinations led to improved consumption patterns. The findings suggest that providing a balanced and varied diet consisting of fruits and seeds is crucial for optimizing bayan parrots' nutritional intake and health in captivity. This study contributes to improved feeding strategies for captive management and conservation efforts, ensuring the well-being of this endemic species.

Polypropylene Packaged Ten Days Cold Stored Rose Cut Flowers cv. Bordeaux Influenced by Different Pulsing Solution

The study included different pulsing solution viz., water, HQC 200mg/l, sucrose 50g/l + HQC 200 mg/l, citric acid 200mg/l, α-lipoic acid 200mg/l, GA3 100 mg/l and BA 100mg/l to study the effect on flower quality and vase life of PP (polypropylene) packaged cold storage (at 2°C) rose cv. Bordeaux, for the period of 10 days. Pre-storage pulsing of GA3 100mg/l solution treatment showed highly promising results in extending flowers vase life along with maintained flower quality just after storage and at 4th DAS in vase life. Further, GA3 100mg/l solution treatment recorded significantly higher water up take (175.65 ml), change in fresh weight (10.50%) at 4th DAS, total dissolve solids (8.85 °brix, 8.05 °brix and 6.75 °brix) and membrane stability index (81.97 %, 65.97 % and 37.88 %) at 2nd, 4th and 6th DAS. Moreover, it was evident from the results that same treatment recoded significantly higher total soluble sugar (59.65 mg/g fresh weight), Estimated protein (18.79 mg/g fresh weight), catalase activity (20.42 µ mol H2O2 min-1 g-1 protein), peroxidation activity (40.30 µ mol H2O2 min-1 g-1 protein), pigment content (15.82 mg/g fresh weight) anthocyanin content in the petals at 4th DAS. Whereas, GA3 100 mg/l pulsing solution treated PP packaged cold stored rose cut flowers cv. Bordeaux recorded significantly higher vase life (6.88 days) which was at par with treatment P2 (Sucrose 50 g/l + HQC 200 mg/l).

PENGARUH EKSTRAK BUAH BELIMBING WULUH (AVERRHOA BILIMBI L.) TERHADAP DAYA TETAS TELUR IKAN GURAMI

Carp is one of commodities freshwater many developed by the cultivator of the fish, as the demand for a high market, maintenance easy and princes relatively stable, think nice and nutrition content high. One alternative to address the problem is the use of extract fruit carambola wuluh. Of which it has infected a fungus saprolegnia sp. Marinated to extrac the fruit of the leatherback wuluh. The concentration of an extract to the leatherback wuluh that is used is identification ppm 4.000 to the density of the 50 egg. The research uses the experimental methods random design it is the complete (RAL) with 4 treatment and 3 preparation of test questions so that apol corporate secretary ronald naggol there were a total of 12 of the experiment. Treatment P1 (time of soaking 10 minutes), treatment P2 (time of soaking 15 minutes), treatment P3 (time of soaking 20 minutes), treatment P4 (in the absence of soaking / control). The research results show that the addition of an extract the fruit of the leatherback wuluh to exert an influence real (P<0,05) against hatch power has risen and other eggs and concern only the survival of roasted gouramy fish with a long time of soaking an extract the fruit of the leatherback wuluh as longaudience with the value of hatch power has risen and other eggs as much as (96.67%). and the level of concern only the survival of (97.93%)

Assessing the Influence of Long-Term Gender-Affirming Hormone Therapy on Cardiovascular Risk in Transgender Men through Carotid Intima-Media Thickness.

Background: Transgender men use exogenous androgen for male pattern virilization. Hysterectomy and bilateral salpingo-oophorectomy (HBSO) is performed to stop the endogenous estrogen secretion. Cardiovascular disease (CVD) risk has been shown to increase with long-term use of androgens and the removal of estrogen. We aimed to investigate the CVD risk in these individuals by measuring internal and common carotid artery intima-media thicknesses (CIMT). Methods: In this cohort study, data were collected from transgender men who had undergone HBSO and used androgens for at least two years (median treatment duration was 5 years in our research). Cisgender women in the same age range were selected as the control group. Demographics, vital signs, and hematological values of transgender patients and cisgender women subjects in the control group were noted. CVD markers were compared with sonographically measured CIMT values. Results: The mean age and body mass index (BMI) of the study group were 32.6 and 25.3, respectively. Weight, systolic-diastolic blood pressure, hemoglobin, hematocrit, low-density lipoprotein (LDL), serum triglyceride (TG), HbA1c levels, internal CIMT, and common CIMT values of the study group were higher, while the high-density lipoprotein (HDL) level was significantly lower compared the control group (p1 = 0.025, p2 = 0.010, p3 = 0.002, p4 = 0.001, p5 = 0.001, p6 = 0.012, p7 = 0.008, p8 = 0.007, p9 = 0.013, and p10 = 0.001). There was also an increase in the body weight, BMI, LDL, and TG levels of the study group after the testosterone treatment (p1 = 0.025, p2 = 0.019, p3 = 0.001, p4 = 0.001, and p5 = 0.001). Conclusions: We demonstrated that the use of testosterone therapy in transgender men is associated with higher CIMT values. While further investigation is needed to assess morbidity and mortality rates, we recommend that regular clinical and radiological examinations be performed in these individuals to accurately evaluate the risk of CVD.

PENGARUH FREKUENSI PEMBERIAN VITAMIN E TERHADAP POST PARTUS ESTRUS DAN POST PARTUS MATING SAPI PERANAKAN FRIESIEN HOLSTEIN

This study aimed to determine the effect of the frequency of vitamin E application on the reproductive performance of postpartum FH crossbreed cows, which Include Post Partus Estrous (first estrus after calving) and Post Partus Mating (first mating after calving). The study used treatment giving vitamin E to cows after calving with treatment 0 (P0)/control, treatment 1 (P1) was injected on the 7th and 14th days after calving and treatment 2 (P2) was injected on the 7th day, the 14th day after calving and 17th day after calving. Post Partus Estrous (PPE) and Post Partus Mating (PPM) were the treatment variables observed. Giving vitamin E on the 7th, 14th, and 17th days after calving (P2) showed a very significant effect (P<0.01) on Post Partum Estrous (PPE). The results of ANOVA analysis of treatment 2 (P2) days 7, 14, and 17 after calving showed a very significant effect (P<0.01) on Post Partus Mating (PPM). This study shows that the P2 treatment with vitamin E injection on days 7, 14, and 17 after calving obtained the best reproductive performance by producing the shortest Post-Partus Estrus (PPE) of 51 days and the shortest Post-Partus Mating (PPM) of 71.83 days.

Management of soft rot in pakchoi (Brassica chinensis L.) using polyphenols from pineapple peel waste

Soft rot is one of the most susceptible soil-borne diseases of pakchoi (Brassica chinensis L.), severely hindering its industrial development. The research aimed to evaluate the application effect of polyphenols extracted from pineapple peel waste in improving the rhizosphere soil environment of pakchoi infected by soft rot and promoting plant health and growth. We found that using pineapple peel polyphenols (PPPs) at 4 mg/mL concentration (P1 treatment) significantly improved soil quality, reduced pakchoi disease incidence, and effectively promoted pakchoi growth. Compared with the control group (CK), the P1 treatment led to a marked 79.97 % reduction in diseased pakchoi leaves. Meanwhile, the rhizosphere soil structure showed reduced diseased genera and increased beneficial bacterial phyla, along with boosted soil enzyme activities. Furthermore, P1 treatment improved key growth parameters of pakchoi, including neck thickness (62.07 %), plant height (57.43 %), fresh weight (154.52 %), dry weight (121.88 %), maximum leaf length (47.28 %), width (30.98 %), and total chlorophyll content (50.11 %). The application of PPPs has shown significant effects in enhancing the ecological environment of the diseased soil and reducing the incidence of soft rot disease of pakchoi and has great potential in increasing the yield of pakchoi. This study offers an eco-friendly and sustainable approach for the valorization of PPPs and the management of soil-borne diseases, which holds profound implications for enhancing crop quality, soil health, and human well-being.

Effect of Pinching on Growth, Yield, and Quality of African Marigold (Tagetes erecta L.) cv. Pusa Narangi Gainda

Marigold is a very common and a hardy flower crop cultivated throughout the world. Pinching is an important intercultural practice commonly followed in cultivation of marigold however, this needs to be standardized for getting higher yields in commercial cultivation. Hence, the present investigation was conducted at Rajasthan Agricultural Research Institute, Durgapura, Jaipur, Division of Horticulture, during 2020-21 and 2021-22 to standardize the commercial pinching practice for getting higher productivity. Three levels of pinching (no pinching, single at 20 days and double at 20 and 40 days after transplanting) were used in the course of the research arranged using a factorial randomized block layout with three replications applied to African marigold variety Pusa Narangi Gainda. The results revealed that treatment P0 (No pinching) was discovered to be the finest in increment in height of plant, early flower bud initiation flower opening and treatment P2 (Double pinching) in boosting spread of plant, primary branches, length of leaf, leaf width, leaf area, plant fresh weight, plant dry weight, total biomass, root- shoot ratio, 50% flowering, flower diameter, stalk and ray floret length, fresh and dry flower weight, mass and number of flower per plant, flower yield additionally improved quality characteristics like chlorophyll and xanthophyll content in marigold. Hence, it might be concluded that application of double pinching can be helpful and recommended in improving yield as well as quality characteristics in commercial cultivation of marigold.

Plant Growth-promoting Potential of Oil Palm Boiler Ash-based Organomineral Fertilizer for Green Mustard Cultivation Under Reduced Urea Dose in Ultisols

This study aims to demonstrate the capability of Oil Palm Boiler Ash (OPBA)-based organomineral fertilizer (OMF) to maintain the growth, green biomass yield, and N uptake of green mustard under a reduced N application rate. This research was carried out from March to May 2021 in a research area in Bengkulu City with a Randomly Complete Block Design scheme and three replications. The treatment consisted of P0 = control (without OMF or urea), P1 = 200 kg urea ha−1, P2 = 325 kg OMF ha−−1 + 50 kg urea ha−−1, P3 = 650 kg OMF ha−−1 + 50 kg urea ha−−1, P4 = 975 kg OMF ha−−1 + 50 kg urea ha−−1, and P5 = 1300 kg OMF ha−−1 + 50 kg urea ha−−1. The results showed that the growth and green biomass yield increased as the application rate of OMF increased. Treatment of P5 produced the highest growth, green biomass yield, and N uptake, equivalent to P2 treatment. The green biomass yield at P5 was 161% higher than that at P0. Therefore, OMF has the potential to promote the agronomic performances of green mustard under 50% reduced N dose in Ultisols. Keywords: slow release fertilizer, nutrient management, oil palm waste, organic pellet fertilizer, green mustard

An operational discontinuous Galerkin shallow water model for coastal flood assessment

Hydrodynamic modeling for coastal flooding risk assessment is a highly relevant topic. Many operational tools available for this purpose use numerical techniques and implementation paradigms that reach their limits when confronted with modern requirements in terms of resolution and performances. In this work, we present a novel operational tool for coastal hazards predictions, currently employed by the BRGM agency (the French Geological Survey) to carry out its flooding hazard exposure studies and coastal risk prevention plans on International and French territories. The model, called UHAINA (wave in the Basque language), is based on an arbitrary high-order discontinuous Galerkin discretization of the nonlinear shallow water equations with SSP Runge–Kutta time stepping on unstructured triangular grids. It is built upon the finite element library AeroSol, which provides a modern C++ software architecture and high scalability, making it suitable for HPC applications. The paper provides a detailed development of the mathematical and numerical framework of the model, focusing on two key-ingredients : (i) a pragmatic P0 treatment of the solution in partially dry cells which garantees efficiently well-balancedness, positivity and mass conservation at any polynomial order; (ii) an artificial viscosity method based on the physical dissipation of the system of equations providing nonlinear stability for non-smooth solutions. A set of numerical validations on accademic benchmarks is performed to highlight the efficiency of these approaches. Finally, UHAINA is applied on a real operational case of study, demonstrating very satisfactory results.

Effect of Rabbit Urine Bio Fertilizer and NPK on Vegetative Variables of Sorghum (Sorghum bicolor L.)

Sorghum (Sorghum bicolor L.) is a plant that requires more N nutrients than other nutrients. The abundance and affordability of rabbit urine bio fertilizer can reduce the cost of cultivation production inputs due to the high price of inorganic fertilizers. Rabbit urine contains higher N nutrients compared to other livestock urine. This study aims to obtain a combination of Rabbit Bio Urine (BUK) fertilizer concentration and NPK fertilizer dosage that can give the best results on the growth of sorghum plants (Sorghum bicolor L.). The research method used is a non-factorial Randomized Group Design (RAK) consisting of 6 levels, namely: P1 (BUK 0 ml/L + 100% NPK), P2 (BUK 41 ml/L + 80% NPK), P3 (BUK 27 ml/L + 80% NPK), P4 (BUK 18 ml/L + 80% NPK), P5 (BUK 9 ml/L + 80% NPK), and P6 (BUK 5 ml/L + 80% NPK). The application of rabbit bio urine fertilizer gives a real effect on the parameters of plant height and stem diameter at the age of 36 HST and 42 HST. In the parameter of the number of leaves, rabbit bio urine fertilizer and NPK gave no significant effect at all observation times. The P2 treatment (BUK 41 ml/L + NPK 80%) gave the highest results in the parameters of plant height at the age of 36 HST and 42 HST which amounted to 98,1 cm and 123,7 cm. In the parameter of stem diameter, the highest result was obtained by P1 (BUK 0 ml/L + NPK 100%) which amounted to 14,8 mm. Keywords : N Nutrient, Rabbit Urine Bio Fertilizer, Sorghum

The Effect of Mocaf Flour and Yellow Pumpkin Flour (Cucurbita Moschata) Proportion on the Acceptability and Physicochemical Quality of Wet MI

Overcoming dependence on wheat flour as raw material, researchers want to develop a wet noodle product by using mocaf flour to substitute wheat flour and adding pumpkin flour to increase the nutritional value of wet noodles, namely potassium minerals as an alternative main food that can help lower blood pressure in hypertension sufferers.To determine the effect of the proportion of mocaf flour and pumpkin flour on the physicochemical quality of wet noodles. This type of research uses an experimental study design with a Completely Randomized Design (CRD). The comparison of the proportions of mocaf flour and pumpkin flour is P0 (100g : 0g), P1 (95g : 5g), P2 (90g :10g), P3 (85 g :15g), P4 (80g :20g). Physical quality and chemical quality test data were analyzed using the Independent T-test. The research results show that the percent water absorption capacity; percent elongation; potassium levels; and sodium levels, namely P0 (58.5% ; 19.8% ; 16.5mg/100 g; 5.64 mg/100g), P2 (70.3% ; 8.8% ; 55.95 mg/100g ; 5.55 mg/100g). The best treatment P2 does not meet the adult RDA percentage and cannot be classified as high in potassium. Wet noodle products cannot yet be used as an alternative to foods high in potassium for preventing hypertension.

Comparative Biochemical and Pharmacodynamic Analyses of Asarum heterotropoides Fr. Schmidt var. Mandshuricum (Maxim) Kitag and Asarum sieboldii Miq var. Seoulense Nakai Roots

Background:Asarum heterotropoides and Asarum sieboldii are commonly used in traditional Chinese medicine. However, little is known about how they differ in terms of essential oil (EO) and ethanol extract (EE) content and composition. Moreover, the effect of various geographical locations on the essential oil (EO), ethanol extract (EE), and asarinin content of different Asarum samples remains unknown. We tested four root-drying methods, i.e., soil removal and shade drying (P1), water washing and shade drying (P2), and water washing and drying at 30 °C (P3) and 40 °C (P4). We used LC-MS and GC-MS to investigate these differences. We also investigated the pharmacodynamic effects of EO and EE. Results: Overall, the EO, EE and asarinin contents of the analysed samples were 19.21–51.53 μL.g−1, 20.00–45.00 μL.g−1, and 1.268–2.591 mg.g−1, respectively. P1 treatment yielded the lowest volatile oil content compared to the other three treatments. GC-MS analysis revealed 78 EO components. Among the six major EO components, eucarvone, 3,5-dimethoxytoluene, and methyl eugenol were higher in A. heterotropoides than in A. sieboldii. However, the latter had a higher myristicin content. LC-MS analysis identified 888 EE components in roots and leaves of A. heterotropoides and A. sieboldii; 317 differentially accumulated metabolites were identified. EO and EE showed a dose-dependent reduction in the degree of swelling and an increase in the inhibition rate of drug concentration on acetic acid writhing in mice. Asarum EO proved to be more effective than EE in the pharmacodynamic study. Conclusions: We conclude that Asarum species show inter- and intra-specific differences in EO and EE content and composition, which may influence the pharmacodynamics of Asarum root extracts.

Microbial Quality of Kupang Paste Products from Different Producers

General Background the utilization of local ingredients, such as kupang, is integral to the culinary practices in Balongdowo Village, Sidoarjo Regency. Specific Background shrimp paste, a prominent product derived from kupang, is valued in the market, yet its quality raises concerns among consumers and producers. Knowledge Gap despite its economic significance, limited research has been conducted on the microbiological quality of kupang-based products, particularly during storage. Aims this study aims to evaluate the microbiological analysis of two kupang petis products from different producers in Balongdowo Village during ambient temperature storage. Results employing descriptive analysis across five storage treatments (P0: fresh, P1: 1 week, P2: 2 weeks, P3: 3 weeks, P4: 4 weeks), findings indicated that total plate count (TPC) in P0 treatment met national quality standards (SNI 2718.1: 2013), while yeast mold levels exceeded permissible limits. Notably, the TPC of sample B (1.6 x 10³) was lower than that of sample A (2.2 x 10³) in the fresh condition (P0). Novelty this research provides a comprehensive analysis of the microbiological quality of kupang petis, filling a significant gap in the literature. Implications the results underscore the necessity for rigorous quality control measures in the production and storage of shrimp paste to ensure consumer safety and compliance with health standards. Highlights: Microbiological quality assessment of kupang petis during storage. TPC values in fresh condition meet national standards. Yeast mold levels exceed permissible limits in stored samples. Keywords: Kupang, Shrimp Paste, Microbiological Analysis, Quality Standards, Storage

The Effectiveness of Tawandakulo (Kleinhovia Hospita L.) On SGOT, SGPT Enzyme Levels, and Hepatocyte Regeneration in Paracetamol-Induced Rats

Background:Paracetamol is metabolized by cytochrome P450 enzymes into a highly toxic reactive metabolite, N-acetyl-p-benzoquinonimine (NAPQI), which can cause liver damage. Antioxidants play a crucial role in mitigating liver toxicity. Kleinhovia hospitaL. (also known as Tawandakulo) is a potent plant antioxidant due to its content of secondary metabolites, such as phenolics, flavonoids, saponins, and terpenoids. Objective:This study aimed to investigate the levels of SGOT and SGPT enzymes, as well as liver histopathology, as indicators of liver health. Methods:An experimental research design with a randomized post-test control group was used. The study involved 25 rats, randomly divided into five groups. Paracetamol was administered at a toxic dose of 2.5 g/kg body weight, dissolved in distilled water, and given orally through NGT no. 5. Results:The study found a decrease in SGOT levels in the treatment group and a significant reduction in SGPT enzyme levels between pre-intervention (P2) and post-intervention (P3). There was a significant change in enzyme levels in treatment group 2 (p = 0.00) and treatment group 3 (p = 0.03). However, no significant difference was observed in treatment group 4 (p = 0.09). Liver cells in treatment groups P1, P2, and P3 showed signs of regeneration, whereas cells in the control group did not regenerate. Group P4 exhibited slower liver cell regeneration than the other treatment groups. Conclusion:This study concludes that a decoction of Kleinhovia hospitaL. can stimulate liver cell growth and reduce SGOT and SGPT enzyme levels. Keywords: Kleinhovia hospitaL; SGOT; SGPT; Liver cell regeneration; Indonesia.

The Effect of Probiotic Administration with Different Time Intervals on Water Quality in Shrimp Cultivation Vaname (Litopenaeus vannamei) Biofloc System

Vaname shrimp is one of the marine fishery commodities that has high economic value. Water in vaname shrimp cultivation must be maintained in quality. Ammonia can be harmful to shrimp, control of ammonia and organic matter content can be done biologically with the application of probiotics. Therefore, this study needs to be conducted to determine the right time interval in providing probiotics to the water quality of vaname shrimp cultivation in the biofloc system. The method used in this study is the experimental method, using RAL with 4 treatment levels and 3 repetitions, with treatment P0 (Once during maintenance), P1 (once every 5 days), P2 (once every 10 days) and P3 (once every 15 days), the probiotic used in this study is the probiotic Probio-7. The results of the study during 56 days of maintenance showed that the average value of water quality salinity 26.7-27 ppt, temperature 27.7-28°C DO 7.13-7.16 mg/L, nitrate 0.63-0.87 mg/L and Total Ammonia 0.76-0.92 mg/L. Based on the research activities that have been carried out, the interval of probiotic administration has a significant effect on water quality. Probiotic administration can increase the Total Ammonia content and reduce the amount of ammonia in this study. Probiotic administration with an interval of 5 days once (P1) gave a total ammonia value of 0.78 mg/L with an ammonia amount of 0.067 mg/L. P1 also gave the highest growth, absolute weight 5.61 g, absolute length 8.82 cm, SGR 5.779%/Day and FCR 1.47.

Evaluation of the Maize - False basil Cultural Association in the Fight against the Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

In Ivory Coast, maize (Zea mays L.) is the second most cultivated cereal after rice. Despite this, maize cultivation is limited by pest pressures. Apart from the usual pests, a new enemy has arrived on the continent, the fall armyworm, Spodoptera frugiperda. To control this pest, farmers use pesticides, which are not only expensive, but also pose risks to human health and the environment. The objective of this study is to find an alternative solution to chemical control through the cultivation of maize-Ocimum gratissimum (False basil) in two ways. The first consisted in planting False basil around the maize plot as a barrier plant (P1). The second consisted of alternating maize plants with False basil plants (P2). The experimental design used was a one-factor randomized FISHER block with three treatments. The efficacy of the two association modalities was compared to that of control which was maize crop only. The results showed better control of fall armyworm in the combination than control. Of the two combination treatments, it was the P1 treatment that was effective against S. frugiperda compared to the P2 treatment and the control. Thus the infestation rate of treatment P1 (33 %) was lower than that of treatment P2 (50 %) followed by the control (70 %). In addition, it was the P1 treatment that harbored fewer armyworms (8 larvae) compared to the P2 treatment (22 larvae) and the control (35 larvae). The maize-False basil crop combination therefore appears to be beneficial than pure crops as it significantly reduces the infestation rate, damage level and harbors less fall armyworm population.

Steroid hormone-deprived sex reversal in cyp11a1 mutant XX tilapia experiences an ovary-like stage at molecular level

Fish sex is largely influenced by steroid hormones, especially sex hormones. Here, we established a steroid hormone-free genetic model by mutation of cyp11a1 in Nile tilapia, which was confirmed by EIA assay. Gonadal phenotype and transcriptome analyses showed that the XX mutants displayed sex reversal from female to male but with defective spermatogenesis. Despite the sex reversal, the aromatase encoding gene cyp19a1a was continuously expressed in the gonads of the XX mutants, which might be caused by androgen deficiency. Whole-mount fluorescence in situ hybridization and transcriptome analysis showed that the gonads of the XX mutants firstly developed towards ovary but shifted to testis between 10 to 15 days after hatching. Detailed expression analysis of key sex differentiation pathway genes foxl3 and dmrt1 combined with apoptosis analysis revealed transdifferentiation of germ cells from female to male during sex reversal. Rescue experiments showed that both P5 and E2 treatment rescued the sex reversal of cyp11a1 mutant XX fish. Overall, our results revealed a transient ovary-like stage and transdifferentiation of germ cells from female to male in the early gonads of the steroid hormone-deprived cyp11a1 mutant XX fish.