Urea Treatment Research Articles

Iron (Fe) and zinc (Zn) coated urea application enhances nitrogen (N) status and bulb yield of onion (A. cepa) through prolonged urea-N stay in alkaline calcareous soil

Onion (A. cepa) is an important horticulture crop which is sensitive to nutrient limitations worldwide. Urea hydrolysis to ammonia (NH3) and carbon dioxide (CO2), due to soil urease activity, causes urea-N loss. Comparative to several commercial products, the potential use of micronutrient coated urea to reduce urea hydrolysis based NH3-volatilization losses is novel to-date. In this study, boron (B), iron (Fe), zinc (Zn) and copper (Cu) were coated on urea at their full or half recommended rates as (B)-half, (Fe)-half, (Zn)-half, (Cu)-half, (B)-full, (Fe)-full, (Zn)-full, (Cu)-full, (B+Fe)-half, (B+Zn)-half, (B+Cu)-half, (Fe+Zn)-half, (Fe+Cu)-half, (Zn+Cu)-half, (B+Fe)-full, (B+Zn)-full, (B+Cu)-full, (Fe+Zn)-full, (Fe+Cu)-full and (Zn+Cu)-full having gum-only coated urea as an internal control. Parallel to these, p-Phenylenediamine (PPD-0.2 %, 0.4 %, 0.6 %, 0.8 % and 1.0 %) and hydroquinone (HQ-0.5 %, 1.0 % and 2 %) coated urea treatments were also used with acetone-only coated urea as an internal control. Whereas the uncoated urea treatment was taken as an overall control. In pot experiment, the soil residual urea concentrations were increased by 2, 0.5, 1.85, 2.69 and 3.15 folds at 8 days after incubation (DAI), by 5.6, 1.89, 5.97, 0.54 and 6.23 folds at 12 DAI and by 119, 123, 155, 84 and 87 folds at 16 DAI under (B)-full, (B+Zn)-full, (Fe+Zn)-full, PPD-0.8 % and PPD-1.0 % coated urea treatments, respectively, as compared to uncoated urea. Among these five coating combinations, 54.26 % and 44.82 % bulb yield increments were observed under (Fe+Zn)-full and PPD-1 % coated urea, respectively, in comparison to uncoated urea in field experiment. However, the 33.33 % increment in nitrogen agronomic efficiency (NAE) and subsequent economic gains proved (Fe+Zn)-full coated urea application as a solution to address N losses in alkaline calcareous soils.

Evaluation of Crop Establishment Methods and Nano Urea Versus Conventional Urea on Growth Indices in Rice Crop (Oryza sativa L.)

The present study aimed to evaluate Crop Establishment Methods and Nano Urea Versus Conventional Urea on Growth Indices in Rice Crop (Oryza sativa L.). Method of crop establishment influences the performance of rice through its effect on growth and development. Although, system of rice intensification has been reported to be the best establishment method. In traditional fertilizers, there is a huge loss of fertilizers by various manners like drift, leaching, runoff, microbial degradation, hydrolysis and photolysis. Bio accessibility of nutrients to plant is much lower relative to the rate of conventional fertilizers applied. The experiment was conducted during two consecutive seasons of Kharif 2022 and 2023 at the Agronomy Research Farm, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya (U.P.), India. The nitrogen level had significantly affected on crop growth rate, relative growth rate and net assimilation rate at all the stages of crop growth during both the years of investigations. The maximum Net assimilation rate at 30-60 and 60-90 DAS/DAT and crop growth rate, relative growth rate at 30-60, 60-90 and 90 to harvest DAS/DAT was recorded under 60% RDN through conventional Urea + 40% RDN through Nano Urea which was at par with 40% RDN through conventional Urea + 60% RDN through Nano Urea treatment, while significant over rest of the treatment during both the years of investigations.

Porous g-C3N4-based nanoarchitectures by playing with sustainable precursors: Role of urea/melamine ratio on the structure/properties relationship

In this study, porous g-C3N4 based nanoarchitectures were obtained by a facile and effective in-situ synthesis method, that entailed the thermal treatment of different melamine (M) and urea (U) precursor mass ratios (in particular M/U = 50:50, 20:80), which were then compared with g-C3N4 obtained from pure M and U. The role of the melamine/urea mass ratio on morphology, structure, surface area, porosity, optical and vibrational properties of the g-C3N4 -based materials, was analysed in detail by means of many characterization techniques, including FESEM, AFM , XRD, HRTEM, Raman, FTIR, UV and PL spectroscopies, BET and TGA analyses. It will be shown that samples obtained from different M and U precursor mass ratios combine the advantage of high thermal stability and significant reaction yields, which are due to melamine action, and greatly improved surface area and short migration paths, which are due to urea effect, thus providing the suitable conditions for charge transfer within the interfaces. The results may shed light on effective improvements in the structure/properties of g-C3N4 based nanoarchitectures, aiming at peculiar functional materials.

Extraction of the outer membrane protein pertactin from Bordetella pertussis with urea for the production of acellular pertussis vaccine

Pertactin (PRN), a non-fimbrial outer membrane protein of Bordetella pertussis, is the limiting component of the acellular pertussis vaccine because of its low concentration. This study aimed to develop a large-scale urea-based process for PRN extraction from B. pertussis. Cell pellet processing conditions, including freezing and thawing, were found to substantially affect PRN yield. A single cycle of rapid freezing of the cell pellet at − 30 °C with slow thawing at 5 ± 3 °C resulted in up to fivefold higher PRN yield than condition without freezing and thawing. The search for urea treatment conditions was also conducted, and 5 M urea treatment for 2 h was the optimal condition. The developed urea-based process was applied to 50 L culture scale, and residual impurities were removed by sequential anion exchange, hydrophobic interaction and gel filtration chromatography and resulted in PRN with a purity of over 95% at a yield of 33.2%. From 50 L culture broth, the final yield of PRN per cell pellet was 0.23 mg/g (wet weight). Thus, a large-scale production process for high-quality PRN from B. pertussis was developed based on urea extraction process. The results may serve as a reference for production of other membrane proteins.

Cereal-Legume Mixed Residue Addition Increases Yield and Reduces Soil Greenhouse Gas Emissions from Fertilized Winter Wheat in the North China Plain

Incorporating crop residues into the soil is an effective method for improving soil carbon sequestration, fertility, and crop productivity. Such potential benefits, however, may be offset if residue addition leads to a substantial increase in soil greenhouse gas (GHG) emissions. This study aimed to quantify the effect of different crop residues with varying C/N ratios and different nitrogen (N) fertilizers on GHG emissions, yield, and yield-scaled emissions (GHGI) in winter wheat. The field experiment was conducted during the 2018–2019 winter wheat season, comprising of four residue treatments (no residue, maize residue, soybean residue, and maize-soybean mixed residue) and four fertilizer treatments (control, urea, manure, and manure + urea). The experiment followed a randomized split-plot design, with N treatments as the main plot factor and crop residue treatments as the sub-plot factor. Except for the control, all N treatments received 150 kg N ha−1 season−1. The results showed that soils from all treatments acted as a net source of N2O and CO2 fluxes but as a net sink of CH4 fluxes. Soybean residue significantly increased soil N2O emissions, while mixed residue had the lowest N2O emissions among the three residues. However, all residue amendments significantly increased soil CO2 emissions. Furthermore, soybean and mixed residues significantly increased grain yield by 24% and 21%, respectively, compared to no residue amendment. Both soybean and mixed residues reduced GHGI by 25% compared to maize residue. Additionally, the urea and manure + urea treatments exhibited higher N2O emissions among the N treatments, but they contributed to significantly higher grain yields and resulted in lower GHGI. Moreover, crop residue incorporation significantly altered soil N dynamics. In soybean residue-amended soil, both NH4+ and NO3− concentrations were significantly higher (p < 0.05). Conversely, soil NO3− content was notably lower in the maize-soybean mixed residue amendment. Overall, our findings contribute to a comprehensive understanding of how different residue additions from different cropping systems influence soil N dynamics and GHG emissions, offering valuable insights into effective agroecosystems management for long-term food security and soil sustainability while mitigating GHG emissions.

RESPUESTA AGRONÓMICA DEL FRIJOL (Phaseolus vulgaris L.) A LA APLICACIÓN DE BIOFERTILIZANTES PROVENIENTES DE RESIDUOS PECUARIOS EN CHIAPAS, MÉXICO

<p><strong>Background:</strong> Beans (<em>Phaseolus vulgaris</em> L.) are an integral part of the basic diet in Mexico and are one of the most important crops. Mexico is the seventh producer worldwide and one of the main consumers of it. Within Mexico, the state of Chiapas is the sixth-largest producer with 68,515 t per year, with a yield of 0.59 t ha<sup>-1</sup>. However, the excessive use of chemical fertilizer has degraded soil quality and increased production costs. <strong>Objective:</strong> To study the agronomic response of the bean crop treated with biofertilizers originated from livestock excreta through anaerobic digestion (effluents). <strong>Methodology:</strong> Variables plant growth variables seed germination, flowering, photosynthesis, and the presence of pathogens were compared. The treatment evaluated were soils treated with bovine manure (treatment 1) and swine manure (treatment 2) after anaerobic digestion, urea (treatment 3) as chemical fertilizer, and control without fertilizers (treatment 4). <strong>Results:</strong> In general, the use of biofertilizers improved the yield per plant, as well as accelerated the development of the plants compared to the control, which was attractive for the producers. In addition, the use of biofertilizers in the soil decreased the presence of pathogens and increased the microbial population, which can be beneficial in soil recovery. <strong>Implications:</strong> The cost of biofertilizer production was 13.5 USD for m<sup>3</sup>, which was cheaper than chemical fertilizers. However, a detailed analysis of the application costs of biofertilizers is recommended. <strong>Conclusion:</strong> The practice of using livestock effluents (biofertilizer) is proposed as a complement to agroecological practices to improve agricultural production and recover soil fertility in southern Mexico. </p>

#794 LncRNA NEAT1 protects uremic toxin-induced intestinal epithelial barrier injury by regulating miR-122-5p/Occludin axis

Abstract Background and Aims Long non-coding RNA(LncRNA) has been reported to be associated with intestinal barrier damage. The aim of this study was to explore the mechanism of lncRNA Nuclear enriched abundant transcript 1 (NEAT1) in uremic toxin-induced intestinal epithelial barrier injury. Method Human colon cancer cells (Caco-2) were used to establish intestinal epithelial injury models with the urea treatment in different conditions. Cell Counting Kit-8 (CCK-8) and Western blot screening the best concentration and time. The expressions of lncRNA NEAT1 and miR-122-5p were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot and immunofluorescence were used to detect the expression of tight junction proteins Occludin, ZO-1 and Claudin-1. Sodium fluorescein was used to detect the paracellular permeability of intestinal epithelial injury models. The binding of miR-122-5p to lncRNA NEAT1 and Occludin was determined by bioinformatics analysis and dual luciferase reporter assay. Results The best condition for the injury model was urea treatment in 144 mg/dl for 48 hours. With the increase of urea intervention time and concentration, the damage degree of intestinal epithelial cells is aggravated. Based on the qRT-PCR results, lncRNA NEAT1 was significantly down-regulated in the model group. Meanwhile, the tight junction proteins Occludin, ZO-1 and Claudin-1 were significantly reduced. The permeability of sodium fluorescein was significantly increased in the model group. Overexpression of lncRNA NEAT1 can alleviate the above performances. As the target gene of lncRNA NEAT1, miR-122-5p is significantly up-regulated in the model group. The dual luciferase reporter assay proved that miR-122-5p was targets to Occludin. The protective effect of overexpression lncRNA NEAT1 on intestinal epithelial barrier function is reversed by miR-122-5p mimics. Conclusion LncRNA NEAT1 protects uremic toxin-induced intestinal epithelial barrier injury by regulating miR-122-5p/Occludin axis.

Synergistic Anti-Inflammatory Activity of Lipid-Free Apolipoprotein (apo) A-I and CIGB-258 in Acute-Phase Zebrafish via Stabilization of the apoA-I Structure to Enhance Anti-Glycation and Antioxidant Activities.

CIGB-258, a 3 kDa peptide from heat shock protein 60, exhibits synergistic anti-inflammatory activity with apolipoprotein A-I (apoA-I) in reconstituted high-density lipoproteins (rHDLs) via stabilization of the rHDL structure. This study explored the interactions between CIGB-258 and apoA-I in the lipid-free state to assess their synergistic effects in the structural and functional enhancement of apoA-I and HDL. A co-treatment of lipid-free apoA-I and CIGB-258 inhibited the cupric ion-mediated oxidation of low-density lipoprotein (LDL) and a lowering of oxidized species in the dose-responsive manner of CIGB-258. The co-presence of CIGB-258 caused a blue shift in the wavelength of maximum fluorescence (WMF) of apoA-I with protection from proteolytic degradation. The addition of apoA-I:CIGB-258, with a molar ratio of 1:0.1, 1:0.5, and 1:1, to HDL2 and HDL3 remarkably enhanced the antioxidant ability against LDL oxidation up to two-fold higher than HDL alone. HDL-associated paraoxonase activities were elevated up to 28% by the co-addition of apoA-I and CIGB-258, which is linked to the suppression of Cu2+-mediated HDL oxidation with the slowest electromobility. Isothermal denaturation by a urea treatment showed that the co-presence of CIGB-258 attenuated the exposure of intrinsic tryptophan (Trp) and increased the mid-points of denaturation from 2.33 M for apoA-I alone to 2.57 M for an apoA-I:CIGB-258 mixture with a molar ratio of 1:0.5. The addition of CIGB-258 to apoA-I protected the carboxymethyllysine (CML)-facilitated glycation of apoA-I with the prevention of Trp exposure. A co-treatment of apoA-I and CIGB-258 synergistically safeguarded zebrafish embryos from acute death by CML-toxicity, suppressing oxidative stress and apoptosis. In adult zebrafish, the co-treatment of apoA-I+CIGB-258 exerted the highest anti-inflammatory activity with a higher recovery of swimming ability and survivability than apoA-I alone or CIGB-258 alone. A co-injection of apoA-I and CIGB-258 led to the lowest infiltration of neutrophils and interleukin (IL)-6 generation in hepatic tissue, with the lowest serum triglyceride, aspartate transaminase, and alanine transaminase levels in plasma. In conclusion, the co-presence of CIGB-258 ameliorated the beneficial functionalities of apoA-I, such as antioxidant and anti-glycation activities, by enhancing the structural stabilization and protection of apoA-I. The combination of apoA-I and CIGB-258 synergistically enforced the anti-inflammatory effect against CML toxicity in embryos and adult zebrafish.

The urea treatment of silver birch (Betula pendula Roth) logs promotes wood decay and wood discoloration

ABSTRACT Silver birch Betula pendula Roth is a widely distributed tree species in northern Europe, the wood of which is extensively used to produce plywood and veneer. However, birch wood is highly susceptible to fungal decay and discoloration. This study investigated the effectiveness of urea treatment against fungi causing discoloration in birch logs. The effect of urea treatment was evaluated in two settings, i.e. in the forest and in the log yard. In the forest, 80 one-meter-long stem segments were produced and half of them were treated with 35% urea solution and the other half with water. To simulate mechanical bark injury, half of the segments were deliberately damaged before storage. In the log yard, 60 stem segments were similarly prepared to assess fungal species colonizing wood during long-term storage. The results showed that urea-treated and bark-damaged logs exhibit the highest rate of wood discoloration, which increased with prolonged storage. Fungal isolation from wood samples identified a higher diversity of fungal species in samples from the log yard. The most frequently isolated fungi were ascomycetes from the genus Ophiostoma. In conclusion, the results demonstrated that the urea treatment is an ineffective measure against fungi causing discoloration in birch wood.

Nitrogen leaching mitigation by tithonia biochar (Tithochar) in urea fertilizer treated sandy soil

Abstract Nitrogenous fertilizer drift from farmlands accelerates nitrogen loads in groundwaters. Biochar potential to mitigate nitrogen leaching in urea treated sandy soil was monitored in a four weeks screenhouse leaching column experiment. The trial was a factorial combination of two biochar types (B1 and B2 applied at 5 t/ha) and two urea treatments (with urea at 120 kg/ha and without urea) laid in completely randomized design with three replications. Control that received neither urea nor biochar was compared. Four weekly leaching events were conducted in each leaching column containing 300 g soil amended with appropriate treatments. Amaranthus hybridus was the test crop. The NH4-N and NO3-N leached were generally highest during the week 2 leaching event such that total NO3-N leached was 427.3 % higher than total NH4-N leached with highest contributions from sole urea treatment. Biochar pretreatment reduced total N leached by 9.5 (B1) and 26.8 % (B2) relative to sole urea. Percentage of N added lost to leaching was highest (34.1 %) in sole urea treatment with B1 and B2 pretreatment reducing the value by 54.5 and 46.9 % respectively. Correlation analysis revealed electrical conductivity of the leachate and soil as dominant indicators for N leached in the soil studied.

The Resource Utilization of Poplar Leaves for CO2 Adsorption.

Every late autumn, fluttering poplar leaves scatter throughout the campus and city streets. In this work, poplar leaves were used as the raw material, while H3PO4 and KOH were used as activators and urea was used as the nitrogen source to prepare biomass based-activated carbons (ACs) to capture CO2. The pore structures, functional groups and morphology, and desorption performance of the prepared ACs were characterized; the CO2 adsorption, regeneration, and kinetics were also evaluated. The results showed that H3PO4 and urea obviously promoted the development of pore structures and pyrrole nitrogen (N-5), while KOH and urea were more conductive to the formation of hydroxyl (-OH) and ether (C-O) functional groups. At optimal operating conditions, the CO2 adsorption capacity of H3PO4- and KOH-activated poplar leaves after urea treatment reached 4.07 and 3.85 mmol/g, respectively, at room temperature; both showed stable regenerative behaviour after ten adsorption-desorption cycles.

Polymer-coated urea applied at one-time mechanical topdressing increases nitrogen use efficiency and rice yield in the cold area

Context The application of slow and controlled release fertilisers can reduce nitrogen loss, but the research is still scarce in the cold rice region of China. Aims The study was conducted to screen slow-release or controlled-release fertilisers which can meet the nitrogen demand of rice and reduce NH3 volatilisation in different pH soils. Methods This experiment includes four fertilisation treatments: no fertiliser (control), urea, urea with N-(n-butyl) thiophosphorictriamide (NBPT), and polymer-coated urea (PCU). The amounts of urea-N, pH, ammonium-N (NH4+-N) in the surface water, and NH3 volatilisation were determined. Thereafter, to evaluate the effects of one-time mechanical topdressing of PCU mixed with compound fertilisers along with farmers’ fertilisation practice (FFP), field experiments carried out in wide soil pH differences were conducted. Key results Cumulative NH3 volatilisation was reduced in the NBPT treatment compared with the urea treatment. There was no significant difference between urea-N, NH4+-N, and pH in the surface water under PCU and control. NH3 volatilisation of PCU is negligible and significantly prolongs the soil nitrogen storage time. In the subsequent experiment, PCU blend increased yield, dry matter, nitrogen uptake, and the partial factor productivity of applied nitrogen (PFPN) compared to FFP. Conclusions PCU treatment slows down urea release and reduces ammonia volatilisation from paddy fields. In turn, it reduces ammonia-nitrogen levels and pH in surface water and further slows down the rate of ammonia volatilisation. Implications Application of PCU as a one-time mechanical topdressing fertiliser in rice fields in cold regions saves nitrogen fertiliser, increases crop yields, and reduces labour.

Urea-treatment of CoOx carbon nanofibers to improve the electrochemical performance of supercapacitor using aqueous electrolytes

Supercapacitors (SCs) play a crucial role in flexible electronics, necessitating innovative approaches to enhance surface faradaic reactions and minimize faradaic diffusion while using aqueous electrolytes. Thus, the urea treatment of cobalt oxide (CoOx)-decorated carbon nanofibers (CNFs) is proposed in this study to decrease the contribution of faradaic diffusion-limited current. Flexible CoOx/CNF electrodes were obtained by annealing ZIF-67-grafted polyacrylonitrile (PAN) fibers via a wet chemical method. The urea treatment of CoOx/CNFs increased the content of sp2-hybridized carbon and pyridinic nitrogen, as confirmed by X-ray photoelectron spectroscopy, effectively enhancing conductivity and pseudocapacitive charge storage capability via nitrogen doping. Notably, urea-treated CoOx/CNF electrode samples exhibited a capacitance of 750 mF cm−2 at a scan rate of 10 mV s−1, while retaining more than 81 % capacitance at a higher scan rate of 100 mV s-1. The cyclic voltammetry curves during variable bending angle testing (0°, 45°, and 90°) exhibited negligible changes, indicating the excellent flexibility of the SCs. The CoOx/CNFs and urea-treated CoOx/CNFs exhibited 80 % and 91 % capacitance retentions, respectively, after 10,000 galvanostatic charge and discharge cycles. Furthermore, the attained energy densities of 76 and 61 µWh cm−2 at the respective power densities of 2 and 20 mW cm−2 indicated the excellent electrochemical performance of the optimal urea-treated CoOx/CNF electrode.

Severe nitrogen leaching and marked decline of nitrogen cycle-related genes during the cultivation of apple orchard on barren mountain

Limited available lands have led to the widespread practice of establishing orchards on barren mountains to expand apple cultivation in China. However, improper nitrogen (N) management strategies in intensive orchards escalate costs and also contribute to environmental contamination. A long-term field experiment was conducted in a representative apple-producing area in Shandong Province, China, to assess the environmental risks associated with newly established orchards. This research utilized lysimeters and soil metagenomic sequencing to investigate N leaching loss and the functional microbial profiles associated with the N cycle during apple cultivation on barren mountains. Five treatments were implemented: no inorganic N fertilizer, traditional farmer's fertilization, drip irrigation with a water-soluble fertilizer, resin-coated urea, and grass interplanting. Among treatments involving inorganic N, the total N leaching rate ranged from 16.5% to 30.7% of the total N fertilizer input. Notably, in treatments with equivalent N fertilizer applications, the water-soluble fertilizer treatment exhibited the highest N leaching loss rate -(20.2–29.0%), while the grass interplanting and resin-coated urea treatments showed significantly lower rates of N leaching loss (17.0–24.9% and 15.2–24.5%, respectively). The abundance of N cycle-related genes experienced a marked decline (17.1–18.8%) during the cultivation of orchards on barren mountains. The water-soluble fertilizer treatment displayed the highest potential for nitrification, contributing to elevated N leaching. Both resin-coated urea and traditional farmer's fertilization treatments exhibited the highest abundance of genes associated with N2O emission risk to the atmosphere. However, the grass interplanting treatment, while effectively reducing N leaching, also triggered the most pronounced soil acidification. Spearman correlation analysis revealed that the decline in pH, coupled with an increase in soil NO3--N concentration and electrical conductivity (EC), were the primary factors contributing to the reduction in the abundance of key N cycle functions. In summary, excessive use of inorganic N fertilizer in orchard environments led to increased N leaching, altered soil physicochemical properties, and significant impacts on N cycle-related genes. The elevated ionic strength in soils might be responsible for both the high N leaching loss and the decrease in N cycle function abundance. In intensive orchard cultivation, each N fertilization strategy mentioned above comes with its environmental drawbacks. Further investigation is warranted to achieve a balance between productivity and environmental protection.

Impact and adoption of feed technologies at Nharira-Lancashire Dairy Scheme

Abstract The objective of this study was to investigate the impact and adoption of nutrition technologies and their effect on milk production at the Nharira-Lancashire dairy scheme. Sixty households (30 from each scheme) were randomly selected and interviewed by use of a semi-structured questionnaire. Fisher’s exact and Monte Carlo tests were carried out to determine the association between farmer experience and feed technology. Logistic regression was used to rank farmers’ awareness and adoption of feed technologies. The results indicated that farmers have been exposed to at least five new feed technologies and adoption is influenced by education status, sex, age and gender of household head (P < 0.05). Feed technology adoption was in the order: silage 70.83% > sunflower cake 41.66% > legume reinforcement 27.1% > legume trees 16.66% > napier fodder 8.33% > urea treatment 4.17%. Milk yield was affected by month and year (P < 0.05). The impact of adoption was below anticipation, as indicated by lack of economic surpluses. A cost-benefit analysis needs to be carried out for all feed technologies within the study area to streamline viable options.

Adjusting photocatalytic hydrogen production of TiO2 hollow nanospheres through nitrogen-induced shallow trapping levels

N-doped titanium dioxide (TiO[Formula: see text] hollow nanospheres with abundant oxygen vacancies were successfully synthesized by coupling urea treatment and annealing in an N2 atmosphere. The pristine TiO2 hollow nanospheres exhibit a shallow donor level for electron trapping, while the urea treatment generates a N 2p acceptor level for hole trapping. After annealing in N2, the sufficient N atoms generate abundant oxygen vacancies for trapping electrons, resulting in further improved charge separation efficiency. The N-doped TiO2 exhibits the highest H2 evolution rate, reaching 2867[Formula: see text][Formula: see text]mol[Formula: see text]g[Formula: see text][Formula: see text]h[Formula: see text], which is six times higher than that of pristine TiO2 hollow nanospheres. The introduction of oxygen vacancies by interstitial N provides a promising way to improve the photocatalysis activity of photocatalysts.

The Difference in Shoot Metabolite Profiles of a Wild and a Cultivated Barley Genotype in Response to Four Nitrogen Forms

Plants can utilize different N forms, including organic and inorganic N resources, and show great differences in the utilization efficiency of each N form among species and genotypes within a species. Previously, we found that the Tibetan wild barley genotype (XZ16) was better in the utilization of organic nitrogen in comparison with the cultivated barley genotype (Hua30). In this study, the metabolite profiles of the two barley genotypes were comprehensively compared in their response to four N forms, including nitrate (NO3−), ammonium (NH4+), urea, and glycine. The macro and micro nutrient concentrations in shoots were mostly found to be higher in the nitrate and urea treatments than in ammonium and glycine in both the genotypes. XZ16 had higher concentrations of nutrient ions in the glycine treatment, but Hua30 accumulated more nutrients in the ammonium treatment. Among a total of 163 differentially regulated metabolites, the highest up-regulation and highest down-regulation values were found in XZ16 in the glycine and urea treatments, respectively. Some important metabolites, such as proline, glutamine, serine, asparagine, L-homoserine, aspartic acid, putrescine, ornithine, and 4-aminobutyrate, were up-regulated in the glycine treatment in both the genotypes with a higher fold change in XZ16 than that in Hua30. Similarly, fructose-6-PO4, aconitic acid, and isocitrate were only up-regulated in XZ16 in the glycine treatment. Here, we concluded that the genotype XZ16 exhibited a better response to the glycine treatment, while Hua30 showed a better response to the NH4+ treatment, which is attributed to the better utilization of glycine-N and NH4+-N, respectively.

UREA TREATMENT OF MATURE WHOLE-CROP CEREAL MIXTURES AS SALVAGE FORAGE FOR SMALL-SCALE DAIRY SYSTEMS IN THE DRY SEASON

<p><strong>Background:</strong> Forage from small-grain cereals is an option to diversify feeding strategies in small-scale dairy systems (SSDS), due to their short cycle and ample adaptation. Mixtures of cereal species may have synergic advantages; however, grazing or ensiling is not always possible due to climatic factors, so forage crops are left to mature and lose quality. Urea treatment of whole-crop mature cereal mixtures may be an option to salvage forage that could not be grazed or ensiled. <strong>Objective:</strong> To evaluate the performance of dairy cows fed urea-treated mature whole-crop forage mixtures of triticale (TRT), rye (RYE), and barley (BLY), in the dry season for SSDS. <strong>Methodology</strong>: Treatment mixtures were TRT+RYE, TRT-BLY, and RYE+BLY. Fully mature cereal mixture crops were treated with 4 - 6% granular urea (plus water) on a fresh basis. Forage variables were analysed with a split-plot design. Whole-crop forage yields were 3.4 ton/ha, with highest yields for RYE-BLY. Crude protein content increased two-fold in treated forages, and dry matter digestibility was moderate with a mean estimated ME content of 7.9 MJ ME/kg DM. Performance was evaluated by an on-farm 3x3 Latin square experimental design repeated 3 times with nine Holstein cows, experimental periods were 14 days, of which 10 days were for adaptation to feeding and 4 days for sampling. Cows were offered daily 9.0 kg DM of urea-treated forage; complemented with 4.4 kg DM of concentrate, day-grazing, and 1.5 kg of cut pasture. <strong>Results: </strong>There were no statistical differences (P>0.05) for any of the animal variables. Urea treatment of whole-crop mature cereal mixtures resulted in a complementary moderate quality salvage forage for the dry season. <strong>Implications:</strong> The present study is presented as an alternative when the crops are in a state of advanced maturation and it is intended to modify the nutritional quality of the forages. <strong>Conclusion:</strong> The use of urea can be an alternative to improve the nutritional quality of forages.</p>

Superhydrophilic Dendritic FeP/Cu3P Electrocatalyst for Urea Splitting via the Intramolecular Mechanism.

The electrocatalytic overall urea splitting can achieve the dual goals of urea treatment and hydrogen energy acquisition. Herein, we exploited the principle of precipitation dissolution equilibrium to obtain bimetallic phosphide FeP/Cu3P/CF for the simultaneous oxidation of urea and reduction of water and comprehensively reveal the inherent molecular thermodynamic mechanisms on the surface of catalysts. The excellent electrochemical performance can be derived from the super water affinity and synergistic effect. Especially, the theoretical calculation unveils that the synergistic effect between FeP and Cu3P can lower the activation energy required for urea electrooxidation, thereby promoting urea splitting. In situ differential electrochemical mass spectrometry (in situ DEMS) measurements further demonstrated that urea oxidation on FeP/Cu3P/CF proceeded according to the intramolecular mechanism. This work has laid the foundation for constructing highly efficient superhydrophilic bifunctional electrocatalysts.

Impact of Foliar Application of Urea and Nano Urea Levels on Quality, Physiological and Leaf Nutrient Content Attributes of Acid Lime (Citrus aurantifolia Swingle) cv. Kagzi in Vertisols of Jhalawar District in Rajasthan

This study attempted the efficacy of various Urea and Nano urea treatments on Kagzi lime fruit characteristics, such as weight, length, breadth of juice recovery, total soluble solids (TSS), acidity, TSS/Acidity ratio ascorbic acid content, and pH value of juices. There were some significant differences in the reaction of Kagzi lime to these treatments. The Urea treatment at 2% (T4) always gave the top values for the fruit weight, length, breadth; juice recovery, TSS content, TSS/Acidity ratio; ascorbic acid content, and juice pH. While the Nano urea treatments showed positive effects, Urea treatment at 2% had the overall better performance. This implies that Urea at this level was superior in improving the fruit quality factors, which may be used to optimize Kagzi lime cultivation methods. In particular, the variables such as fruit characteristics, juice properties and nutritional content, chlorophyll content; relative water contents in leaves of leaf nutrient contents were positively influenced by 2.0% treatment Urea application These protocols led to significant improvements in fruit weight, length, and width being greater as good juice contented and TSS which increased considerably the levels of Ascorbic acid were improved, chlorophyll content enhanced while there were reduced effects despite that resulted from this prophylactic treatment. Moreover, it resulted in the maximum level of leaf nitrogen and phosphorous. These results show that Urea has significant influences on the quality of acid lime cv. The highest favorable results were noticed in the 2.0% Urea treatment administered by Kagzi lime.