Treatment Of Leucoderma Research Articles

Flavonoids extract from the seeds of Psoralea corylifolia L. (PFE) alleviates atherosclerosis in high-fat diet-induced LDLR−/− mice

BackgroundThe seeds of Psoralea corylifolia L., a traditional medicine popular used in China and India, have been recommended in the treatment of leucoderma, psoriasis, osteoporosis, and gynecological bleeding. Our previous studies have found that flavonoid extract from the seeds of Psoralea corylifolia L. could activate fat browning and correct the disorder of glucose and lipid metabolism in obese mice. PurposeThe present study aimed to investigate the anti-atherosclerosis of flavonoids extract from the seeds of Psoralea corylifolia L. MethodsLeukocyte adhesion assay, RT-PCR, Western blot analysis, and immunofluorescent assay were carried out in ox-LDL induced endothelium injury and foam cells formation in vitro. Flavonoids from the seeds of P. corylifolia L. (PFE) was administrated 150 and 300 mg/kg/day in HFD-induced LDLR−/− mice for 12 weeks. ResultsFlavonoids from the seeds of P. corylifolia L. (PFE) could prevent leukocyte adhesion to the endothelium by inhibiting mRNA and protein expression of these adhesion molecules (VCAM-1, ICAM-1, and E-selectin). PFE could also prevent ox-LDL stimulated inflammation in HUVECs by inhibiting the NF-κB pathway. In addition, PFE significantly ameliorated ox-LDL induced macrophages-oriented foam cells formation through inducing cholesterol efflux via PPARγ-ABCA1/ABCG1. In HFD-induced LDLR−/− mice, PFE reversed the serum profile and circulated inflammation level. Meanwhile, PFE could remarkably alleviate atherosclerotic lesion sizes and intraplaque macrophage infiltration in aortic roots. ConclusionFlavonoids from the seeds of P. corylifolia L. could alleviate atherosclerosis by preventing endothelium injury, attenuating vascular inflammation, and alleviating the formation of foam cells.

The Phytochemical and antioxidant activity of calotropis procera

ABSTRCTCalotropis procera (Ait) R.Br.) is one of the most important plant widely cultivated in India,afghanistan and other countries also.It belongs to family Asclepiadaceae.This plant is widely used as antioxidant,antidiabetic,anticancer,antitumour,treatment of leucoderma,ulcer,rheumatoid,arthritis,wound healing jaundice. The plant is also known as arka,ak and madhar.It is also known as milkweed family.The plant every part is medicinal feature.It contains rich source of antioxidant compounds.Antioxidant compounds are significances in prevention onset of major degenerative diseases. The Major role of antioxidant compounds is to scavenge to free radicals.It is highly toxic and an important causative agent of many diseases including cancer, heart disease, cataract and congestive disorders. Antioxidant compounds block the oxidation processes that produce freeradicals which contribute towards these chronic diseases and aging different types of active phytoconstituents like alkaloids, carbohydtates, saponins, tannin,steroids,glycosides,phenlic compounds are present. This plant is also occur in dry region area.The present review discussion in the phytochemical composition,medicinal uses & Antioxidant activity of this plant. Keywords:Calotropis procera,Phytochemical screening ,Antioxidant ,DPPH Scavenging activity,Determination of total phenol content,Determination of total flavonoid content

Anti microbial activity of different dosage forms of Bakuchi (Psoralea corylifolia Linn.) taila, An Ayurvedic formulation

      Psoralea corylifolia Linn. commonly known as “Bakuchi” used in Indian traditional medicine. Ayurveda for the treatment of leucoderma, scabies, leprosy, psoriasis, dermatitis etc. Bakuchi taila is one of the Ayurvedic formulation which is used externally in skin disease. Hence, an attempt has been made to convert Bakuchi taila into its different dosage forms and to evaluate anti microbial activity against gram positive and gram negative bacilli. Bakuchi taila, Bakuchi gel, Bakuchi siktha taila and Bakuchi ointment possess significant anti microbial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia. These results confirmed that potential of Bakuchi seeds (Psoralea corylifolia Linn.) in the development of Ayurvedic topical skin formulations.Â

Chemical profiling and anti-psoriatic activity of methanolic extract of Andrographis nallamalayana J.L.Ellis

Andrographis nallamalayana is being widely used as tribal medicine in the treatment of leucoderma and mouth ulcers. Chemical profiling of methanolic extract of the whole plant (PE), using GC–MS and LC–MS, revealed the presence of compounds viz. α-tocopherol, β-sitosterol, tetradecanoic acid, monostearin, flavones/flavanones and their glycosides, chromones, etc. Topical application of imiquimod on the dorsal portion of male BALB/C mice resulted in the development of psoriatic symptoms (erythema, scaling, thickening and folding) with a mean disease activity index (DAI) of >7.0. Topical treatment with 100-μL PE (~6.4%/12.8%) formulations, for 12-days, resulted in the alleviation of disease symptoms. Compared to water-based formulations, emu oil-based formulation, PE400EO was found more effective in reducing the mean DAI (>84%), keratinocyte count (>65%) (p < 0.01) and interleukin-22 (~70%) (p < 0.05). We report, for the first time, anti-psoriatic activity of A. nallamalayana having great potential in developing a potent phytomedicine against psoriasis.

Pharmaceutical Standardization and Evaluation of Herbomineral Cream (ALG-06) for Leucoderma

The aim of this study was to formulate and evaluate the herbomineral cream ALG-O6 which is used in the treatment of leucoderma. In this formulation we used mineral drugs (red oxide and purified sulphur) along with specific combination of four common medicinal herbs (i.e. Azadiracta indica A Juss. Psoralea corylifolia L., Punica granatum L., Trigonella foenum-graecum L.) in the extract form. The quality of  the cream (ALG-O6) was evaluated by organoleptic features (color, odor and appearance) and on other hand its chemical and  physical characteristics such as pH,viscosity, solubility in different solvents along with  bio burden and irritancy test on the skin was also performed. Cream was observed in the accelerated stability condition at room temperature (RT) and 40°C/75% (RH) for a period of 6 months. Phytochemical screening of herbomineral extract (HME) and standardization were carried out by TLC finger prints of extract with two different solvent systems

Hairy roots of Ruta graveolens L. – a rich source of coumarins and furanocoumarins

Ruta graveolens L. (Rutaceae) is one of the richest natural sources of coumarins (i.a.: umbelliferone) and linear furanocoumarins (i.a.: psolaren, bergapten, xanthotoxin). Ruta has been used in the treatment of leucoderma, vitiligo and psoriasis diseases. The aim of our work was to obtain hairy root cultures of R. graveolens after transformation of explants of in vitro grown plant with Agrobacterium rhizogenes. Hypocotyls were transformed with 2 strains of A. rhizogenes (A4, LBA9402) using 2 methods. Axenic cultures derived from a single root tip were established after 3–5 weeks of subculture in an Erlenmeyer flask containing B5 medium, 3% sucrose, claforan and carbenicillin (500mg l-1). Hairy roots were maintained on a liquid B5 medium without antibiotics (16/8h photoperiod or in the darkness), on a rotary shaker at 110 rpm. Subcultures were made every 4 weeks. The transformation of Ruta tissue was confirmed on molecular level using PCR tests with primers based on the sequence of rolB and rolC genes of A. rhizogenes. Quantitative and qualitative determination of coumarins and furanocoumarins in chloroform and methanol extracts was performed by using a Hewlett- Packard Model 5890 gas chromatograph coupled with mass spectrometer TRIO 3000. Separations were performed on a capillary column BP-1 (30m x 0,25 i.d., 0, 25µm film thickness). The growth index (t 30 /t 1) for control shoots grown in vitro amounted to 5.5 and for hairy roots 4.8. In comparison to control shoots of R. graveolens in hairy roots growing in the dark we detected 2 times more bergapten; 16.5 times more izopimpinellin and 4 times more rutacultin isomer. In addition new compounds were identified, like osthol, 3-(1,1-dimetylallylo)scopoletin, 2,5-dimetyl-7-hydroxychromon, 5-(3-metyl-2-butenyl)-8-metoxypsolaren and chalepin. The level of these compounds in hairy roots growing under a 16h photoperiod was on average 2–3 times lower than in roots growing in the dark. The obtained results indicate that hairy roots of R. graveolens grown in the dark are a rich source of coumarins and furanocoumarins.

Leucodermies

Leucoderma is a whitish discoloration of skin color. We suggest a diagnostic work-up of leucodermas relying on extension of disease and age at onset. The following diseases and entities will be discussed in this chapter : oculocutaneous albinism, other generalized genetic leucodermas, tuberous sclerosis, piebaldism and Waardenburg syndrome, pigmentary mosaicism and achromic hamartoma, vitiligo, exogenous leucoderma, Sutton phenomenon, white tumors, infectious and inflammatory leucodermas as well as post-inflammatory hypopigmentation, progressive macular hypomelanosis, pityriasis alba, idiopathic guttate hypomelanosis, anemic hamartoma and Bier spots. Treatment of leucoderma is discussed and will be focused on the treatment of vitiligo.

Variation in xanthotoxin content in Ammi majus L. cultures during in vitro flowering and fruiting

Ammi majus L. possess various linear furanocoumarins, in particular, xanthotoxin which is used in the treatment of leucoderma. We report a reproducible protocol for micropropagation through mature nodal segments and the variation in xanthotoxin content at various stages of in vitro flowering and fruiting. Direct organogenesis was achieved in 100% cultures on Murashige and Skoog's (MS)+indole-3-acetic acid (IAA) (0.5 mg l −1)+kinetin (Kn) (2.0 mg l −1)+casein hydrolysate (CH) (1000 mg l −1) and flowering and fruiting on MS+IAA (0.5 mg l −1)+Kn (5.0 mg l −1)+adenine (Ad) (40 mg l −1)+CH (500 mg l −1). Shoots were rooted on MS+indolebutyric acid (IBA) (0.2 mg l −1)+glutamine (Glu) (100 mg l −1). Rooted plantlets transferred to soil could be successfully established in pots. Xanthotoxin content of in vitro cultures bearing immature green fruit was 11.9 mg g −1 dry weight in contrast to 4.2 mg g −1 dry weight in natural populations. In vitro-raised plantlets transplanted to field showed 4.8 mg g −1 dry weight of xanthotoxin.

Treatment of vitiligo.

To the Editor: —I have received numerous inquiries about my discussion in theArchives( 63 :786 [June] 1951) of the treatment of vitiligo. In 1947, I. R. Fahmy and Hamed Abu Shady isolated from the fruits of Amni majus two crystalline compounds, to which the names amoidin (C 12 H 8 O 4 ) and ammidin (C 16 H 14 O 4 ) were given. These are really bitter substances and not alkaloids, as they do not contain a nitrogen group. The available literature to date is as follows: Fahmy, I. R., and Abu Shady, H.: The Isolation and Properties of Ammoidin, Ammidin, and Majudin and Their Effect in the Treatment of Leucoderma, J. Pharm. & Pharmacol. 21:449-503, 1948. El Mofti, A. M.: A Preliminary Clinical Report on the Treatment of Leucoderma with Amni Majus Linn, J. Roy. Egyptian M. A. 31:651-665 (Aug.) 1948. El Mofti, A. M.: Further

A note on the preparation and reactions of Karanjin

A method of extracting Karanjin in good yield from Pongamia oil is described. Karanjin is only faintly bitter and is found to be useful for the treatment of leucoderma. A method of hydrolysis of Karanjin giving rise to a good yield of Karanjic acid has been found. It has been shown that Pongamia oil contains potassium in some form of chemical combination. By the action of mercuric acetate, Karanjin yields a di-acetoxy-mercuri compound.