Treatment Of Inflammatory Periodontal Diseases Research Articles

CLINICAL AND LABORATORY EVALUATION OF A NOVEL DENTAL GEL FOR TREATMENT OF CHRONIC CATARRHAL GINGIVITIS

This article presents the study of a dental gel developed by us, containing, as an active ingredient, sanguitrithrin and oil extract from calendula flowers and yarrow grass. As a result of our studies of the dental gel on 1,844 children and adolescents, we revealed that it has antiinflammatory and wound-healing activities and produces anti-inflammatory, reparative, hemostatic and trophic effects. The results of the study make it possible to use the dental gel in clinical practice for treatment of inflammatory periodontal diseases in children. We substantiated the prospects of using the phytocomplex in the form of a dental gel and proved it as effective and safe. Besides, its dosage form is convenient for use in dental practice and even provides advantages over known domestic analogues.

Conservative treatment of inflammatory periodontal diseases in young, middle-aged and elderly patients: current aspects

Conservative treatment of inflammatory periodontal diseases in young, middle-aged and elderly patients: current aspects

ОЦЕНКА КОМПЛЕКСНОГО ЛЕЧЕНИЯ C ИСПОЛЬЗОВАНИЕМ СТОМАТО-ЛОГИЧЕСКОГО ГЕЛЯ ОРИГИНАЛЬНОГО СОСТАВА НА ПОКАЗАТЕЛИ ПСИХОЭМОЦИОНАЛЬНОГО И СТОМАТОЛОГИЧЕСКОГО ЗДОРОВЬЯ КУР-САНТОВ ВЕДОМСТВЕННОГО ИНСТИТУТА

Subject. Dental diseases are a frequent companion of distress, since the negative impact of psychoemotional stress on the immune status of cadets is reflected in changes in the microflora of the oral cavity and periodontal [4, 9]. 
 The aim of the study is to evaluate the results of using the developed gel for the preven-tion and treatment of inflammatory periodontal diseases in cadets who are under psychoemotional stress.
 Methodology. The group of examined persons consisted of 245 people-cadets of 1–4 courses of the Ministry of Internal Affairs of the Russian Federation. After the dental exami-nation, the cadets were divided into 2 groups: 26 cadets were found to have gingivi-tis/periodontitis of mild severity (group I), and 45 cadets without periodontal lesions (group II) served as a comparison group. The study included psychological testing, clarification of the state of their vegetative reactions and cortisol content in saliva, molecular genetic deter-mination of the composition of the most common periodontal pathogenic bacteria of the dentoalveolar furrow and enzyme immunoassay of the content of pro-inflammatory cytokines in saliva, cell-regulatory and immunosuppressive effects. 
 Results.The application of the gel helps to correct the phenomena of depression, while it affects the frequency of relapses of diseases.The effect of the application on the frequency of detection of periodontal pathogenic bacteria in cadets is manifested in relation to Tanerella forsythia and Aggregatibacter actinomycetemcomitans in the absence of depression, but in the presence of periodontal lesions. When the phenomena of gingivitis/periodontitis of mild se-verity occurred against the background of depression, the effects of using dental gel were in the form of a decrease in the occurrence of these bacteria. There was a significant drop in the oral fluid of IL-12 in the group with depression and periodontal disease, as well as IL-10 in the group without depression, but with mild gingivitis/periodontitis.
 Conclusions. The developed dental gel is recommended both for the phenomena of de-pression and in cases of mild gingivitis/periodontitis against the background of psychoemotional stress.

ИЗМЕНЕНИЕ ФАКТОРОВ НЕСПЕЦИФИЧЕСКОЙ ЗАЩИТЫ РОТОВОЙ ПОЛОСТИ У ДЕТЕЙ С ГИНГИВИТОМ НА ФОНЕ ПРИМЕНЕНИЯ ЭФИРНЫХ МАСЕЛ И БЕНТОНИТОВОЙ ГЛИНЫ

Introduction. The prevention and treatment of inflammatory periodontal disease in children remains a topical issue in dentistry. This is due to the increasing prevalence of this disease in the early age groups. Remission periods of diseases in children are short. Therefore, treatment and preventive measures of periodontal tissue diseases in this population should be primarily focused on the regulation of nonspecific protection system at the local level. The components of the system are secretory immunoglobulin A, lysozyme and modified albumin. Using means of natural origin as regulators will allow to optimize treatment regimens and reduce allergic reactions. Aim. The aim of the study was to study the effectiveness of the complex for applications to the gingiva, consisting of means of natural and mineral origin of the Crimean deposit, in the regulation of local oral immunity in children with chronic catarrhal gingivitis. Material and methods. Twenty-eight children aged 11–12 years with moderate chronic catarrhal gingivitis were examined. The control group consisted of 12 healthy children of the same age. We used applications of phytopreparations on the gingiva in the treatment of chronic catarrhal gingivitis daily for 20 minutes, No 10. Complex for applications was essential oils of peppermint, pine, fennel, eucalyptus (1:1:1:1) on the basis of bentonite clay (10:1). Clinical and laboratory parameters were determined in the dynamics. Statistical processing was performed using the standard method of variation statistics. Comparison of the mean indices was conducted using Student’s t-criterion. Differences were considered reliable at p<0,05. Results and discussion. The criterion for the effectiveness of treatment was the positive dynamics of paraclinical indices (p<0,001). Indicators of oral dysbiosis decreased and reached values of oral microbiocenosis of healthy children. The content of sIgA increased 1,8 times (p<0,001). The level of salivary lysozyme activity increased 1,7 times (p<0,001) and reached normal values. The concentration of modified albumin decreased 1,6-fold. Conclusion. Inclusion of applications of the combination of essential oils based on bentonite clay of the Crimean deposit into the plan of treatment of chronic catarrhal gingivitis in children contributes to a pronounced relief of the signs of inflammation in the gingiva. The proposed complex has a pathogenetic orientation in the treatment of chronic catarrhal gingivitis, which is confirmed by the pronounced dynamics of nonspecific immunity parameters.

Фітопрепарати в комплексному лікуванні запальних захворювань пародонту

In this article the review of foreign and domestic literary sources, which are devoted to the actual problem of modern dentistry – the treatment of inflammatory diseases of periodontal tissues: gingivitis and periodontitis are presented. The complex approach to their treatment involves the appointment of a significant amount of pharmacotherapeutic drugs. Therapeutic failures and iatrogenic complications have led to the fact that today the interests of doctors and population to medicinal products significantly increased. The purpose of the study is to analyze the data of scientific literature on the use of plant-based medicinal products for the treatment of periodontal inflammatory diseases over the past 10 years. Materials and methods. Comprehensive and systematic analysis of literature. Review and discussion. The analysis of information sources on the use of plant-based medicinal products in dentistry both independently and in the composition of medical and prophylactic means has established that the modern assortment of plant-based preparations in the pharmaceutical market of Ukraine to a certain extent is limited. The emergence of new plant-based species that have been tested in conditions of experimental pathology and require an evidence-based clinical base is noted. The composition of plant-based preparations used for the treatment of inflammatory periodontal diseases include vitamins, biologically active substances, glycosides, alkaloids, in connection with a wide range of action: antiseptic, anti-inflammatory, regenerating, hemostatic, antioxidative. The data on plant-based preparations that are most often used such as chamomile extracts, calendula, hypericum, plantain, kalanchoe, aloe, eucalyptus, milfoil, nettle, calamus and plant-based species are summarized. The medicinal agents considered are mainly recommended for local treatment of periodontal diseases in the form of dental care means, mouth rinse, gel, chewing gum, herbal liquer. It is known that the complex treatment of periodontal diseases includes a general influence on the body. The properties of green tea with its wide range of actions are investigated. With antioxidant properties, it can be a healthy alternative for controlling destructive changes in periodontal diseases. Attention is drawn to the proposed unique natural complex “Resverazin” due to a wide range of pharmacological action, low toxicity and relative safety. The drug produces antioxidant, anti-inflammatory, immune stimulating, vasodilative, neuroprotective action. Conclusion. Based on the literature analysis, it can be concluded that the accumulated experimental and clinical data on the therapeutic properties of plants prove perspective of their use in the complex treatment of inflammatory periodontal diseases. Future studies are mandatory for further confirmation of the effectiveness of these medicinal plants

METHODS FOR TREATMENT OF CHRONIC GENERALIZED PERIODONTITIS (LITERATURE REVIEW)

The article provides data on the methods used for the treatment of generalized periodontitis. Most researchers believe that periodontitis is a polyetiological disease, the development of which is based on a complex of pathological changes occurring in the oral cavity associated with microbiological and immunological changes. Improving the effectiveness of treatment of inflammatory periodontal diseases is an urgent task in modern dentistry.Keywords:dentistry, diseases of the oral mucosa, periodontal disease, chronic generalized periodontitis, etiology, treatment

ANALYSIS OF THE TREATMENT OF PATIENTS WITH PERIODONTITIS IN THE THERAPEUTIC DEPARTMENT OF THE CLINIC OF DENTISTRY OF THE MILITARY MEDICAL ACADEMY NAMED AFTER S. M. KIROV

Over the past decades, significant progress has been made in the study of the etiology and pathogenesis and in the treatment of inflammatory periodontal diseases, including chronic generalized periodontitis, however, according to epidemiological studies, a high level of prevalence of periodontal diseases among the population of our country remains. Elderly patients sometimes do not fully adapt to the conditions of existence after complex therapy. According to the analysis of the structure of patient’s visits to the therapeutic department of the Clinic of Dentistry of the Military Medical Academy named after S.M. Kirov in the period of 2017-2019, an annual increase in the number of visits for localized and generalized periodontitis was revealed. Periodontitis ranks third or fourth among oral diseases in terms of the number of initial requests for therapeutic dental care, and second in terms of the total number of visits, second only to dental caries, which demonstrates the social significance of this disease. The dynamics of the ratio of primary and repeated visits for periodontitis is of interest. From 1:2.3 in 2017, this is the ratio in 2018. increased to 1:2.7 and reached 1: 3.5 in 2019, which indicates an increase in the quality of dental care for patients with chronic localized and generalized periodontitis. The main measures of non-surgical treatment of periodontitis were professional oral hygiene, correction of individual hygiene skills, local use of antiseptics, which meets the requirements of the clinical recommendations for the treatment of periodontitis, approved by the Decision of the Council of the Association "Dental Association of Russia". At the same time, in the surgical department of the dental clinic, curettage of periodontal pockets was performed in 639 patients in 2017-2018.

Therapeutic physical factors at chronic generalized periodontitis (literature review)

Strengthening public health and increasing the life expectancy of Russians are the most priority tasks of the state.
 Chronic generalized periodontitis occupies one of the leading places among the urgent problems of dentistry. The main therapeutic and preventive measure in this case, through which the preservation and strengthening of the health of the population is achieved, is medical rehabilitation using therapeutic physical factors. The inclusion of therapeutic physical factors in the rehabilitation treatment programs for various chronic diseases, including periodontal diseases, significantly increases the clinical effectiveness of rehabilitation programs, which is associated with the summation and mutual potentiation of their therapeutic effects, comparability and minimal side effects. So, numerous studies on the use of therapeutic physical factors in dentistry convincingly testify to the sanogenetic effects of therapeutic mud, mineral bischofite complexes, mineral waters in the treatment of inflammatory periodontal diseases.
 The rationale for the development of new treatment and rehabilitation technologies for chronic generalized periodontitis is the insufficient effectiveness of the existing standardized methods of treating this category of patients.
 The review article is devoted to the restorative treatment of patients with chronic generalized periodontitis. Indications and contraindications for the use of therapeutic physical factors (balneological methods, pelotherapy, apparatus physiotherapy, climatotherapy, psychotherapy, diet therapy, new forms of balneo- and phytopreparations) for this category of patients are presented. It should also be noted the role of promoting a healthy lifestyle, including training in primary and secondary prevention, in order to preserve the dental health of the Russian population.

Захворюваність населення України запальними захворюваннями пародонта, прогнозування та профілактика патологій в сучасних умовах

The purpose of the study. The article reflects the issues of the incidence of inflammatory periodontal diseases in the population of Ukraine, methods for predicting exacerbations of diseases for the timely conduct of secondary prevention. It has been established that the high prevalence of periodontal diseases among different groups of the population makes this problem one of the most important problems of modern dentistry. According to WHO experts, 80% of schoolchildren in different countries have periodontal pathology, and among adults it occurs in almost 100% of cases. Modern periodontal prophylaxis should be carried out on the basis of clinical examination of patients. Periodontal prophylaxis should be based on taking into account the identified etiological factors of the disease. Predicting the incidence of periodontitis and predicting the course of the disease is one of the most important problems of periodontology today. Solving forecasting problems in clinical, experimental and preventive medicine is one of the most difficult areas of the activity of doctors of various profiles. Materials and methods. In order to form a risk group, the occurrence of an exacerbation of inflammatory periodontal diseases for the timely implementation of secondary prevention of pathology, an algorithm for the probability of diseases and a mathematical model for predicting the course of the disease were developed. The use of this method made it possible to select 9 signs out of 42 that most significantly affect the prognosis of exacerbation in inflammatory periodontal diseases. Results and discussion. Based on the curves of the dependence of the frequency of the occurrence of exacerbation of inflammatory periodontal diseases on the magnitude of the risk of exacerbation of this pathology, 3 degrees of prognosis probability were determined: low, medium, high. The use of the method of stepwise discriminant analysis made it possible to develop a system for predicting the possibility of an exacerbation of inflammatory periodontal disease and to scientifically substantiate the principles of identifying risk groups for the possibility of exacerbation of inflammatory periodontal diseases in this group of examination. For patients with a risk of exacerbation, dynamic observation was established for one year, before the beginning of which they were divided into 2 groups – the study and control. Patients in the study group underwent preventive treatment, which included the appointment of an appropriate diet, work and rest regimen, correction of the state of the immune system using immunomodulatory drugs, treatment of concomitant diseases and local treatment of inflammatory periodontal diseases. Patients in the control group underwent conventional prophylaxis, which included adherence to general rules: a rational regimen, a balanced diet, rational oral hygiene, and basic sanitary and educational work. Conclusion. As shown by the results of dynamic observation, among patients of the study group, exacerbation of the disease was observed in 14.3% of cases, in patients of the control group – in 42.5%, which corresponded to the likelihood of an exacerbation of the disease by 94.5%

The effectiveness of a dental gel with barberry extract in the treatment of plaque-induced gingivitis in orthodontic patients undergoing treatment with fixed appliances

Relevance. The high necessity in orthodontic treatment among young patients and the use of fixed orthodontic appliances for the treatment of dental pathology are the factors that initiate inflammatory periodontal processes. Timely diagnosis and optimal preventive and treatment procedures can prevent or stop the inflammatory periodontal complications which could have developed during the orthodontic treatment. One of the effective methods for the prevention and treatment of inflammatory periodontal diseases is the use of targeted antimicrobial agents based on medicinal plants that have specific antimicrobial activity against periodontal pathogens. Purpose – to study the effectiveness of the dental gel with barberry extract for the treatment of plaque-induced gingivitis in orthodontic patients during the active phase of the treatment with fixed appliances.Materials and methods. The study included 60 patients (35 women and 25 men) aged 18 to 35 years who had presented for the orthodontic care. The patients undergoing orthodontic treatment with fixed appliances were randomly divided into two groups. In group I, the patients were prescribed applications of a dental gel with barberry extract; in group II, this medication was not prescribed. The clinical condition of the periodontium was assessed with periodontal indices PMA, SBI and OHI-S. The immune status was evaluated by the level of secretory IgA, interleukins (IL-1β and IL-4) and tumor necrosis factor-α (TNF-α). The patients were followed up during 1 month of treatment. The results were statistically processed with Statsoft Statistica 8.0 and Graph Pad Prism 5.0.Results. In group II, the production of immunoglobulin IgA, pro- and anti-inflammatory cytokines was impaired after a month of treatment, associated with a significant deterioration in the oral hygiene status and an increase in PMA (by 3 times), SBI (by 5.3 times) indices. In group II, the oral fluid concentrations of IL-1β, IL-4 and TNF-α significantly differed from those in group I as of the 7th day of the observation period and indicated suppression of the mucosal immunity. Clinical and immune parameters of the group I patients improved and were comparable with the control by the end of the observation month.Conclusions. Patients with dental abnormalities are at risk of developing inflammatory periodontal diseases. The results of the immunological tests, performed during the treatment with the use of a dental gel with barberry extract, demonstrated the recovery of the local immunity, as evidenced by the elevation of secretory immunoglobulin A in saliva and improvement of cytokine profile parameters.

MODERN SCIENTIFIC VIEW ON THE OPTIMIZATION OF TREATMENT OF PERIODONTAL DISEASES

The article presents a retrospective review of professional domestic and foreign literature on modern aspects of treatment of inflammatory periodontal diseases, including vector therapy, plasmalifting, which can be used in various forms of this pathology. Periodontal diseases are one of the most pressing problems of dentistry, which has not only general but also great social significance, due to the high prevalence, intensity of the process, the subsequent formation of chronic odontogenic foci of infection, tooth loss at a young age and adverse effects on the body. Treatment of periodontal disease is a complex and lengthy process that includes general methods of influencing the pathophysiological processes occurring in the body, both local - therapeutic, orthopedic and surgical measures. Before planning treatment, the dentist should determine the patient's medical and dental history, conduct clinical and radiological examinations, determine the degree of loss of the alveolar ridge, functional disorders of the dental system and the possibility of their correction. Depending on this, determine the plan of treatment and rehabilitation measures, the sequence of medical interventions. The main task of periodontal treatment is to optimize the conditions for maintaining a healthy dentition and restoring its physiological functions. The difficulty of treating inflammatory periodontal diseases is that known and standard methods (antibacterial therapy and courses of local therapy) do not always give a positive result and a long period of remission. Therefore, to improve the quality of medical care for patients with periodontal pathology, it is necessary to introduce new innovative methods of treatment in dental practice. Given the patterns of pathophysiological processes occurring in periodontal tissues and bone tissue of the alveolar process, to date, several stages of treatment and rehabilitation measures have been proposed. The first stage is the elimination of the pathogenic action of microbial factors and inflammatory processes in periodontal tissues. Microbial invasion and the inflammatory reaction of periodontal tissues caused by it activate the processes of osteoclastical bone resorption. The task of the second stage of treatment and rehabilitation measures is to restore the physiological structure of gums and bone by normalizing metabolic processes in periodontal tissues, reducing the activity of resorption processes and stimulating reparative osteogenesis in the bone tissue of the alveolar process and the body in general, surgical treatment. At the third stage of treatment and rehabilitation measures, teeth with a significant degree of atrophy of the alveolar process are removed, which cannot be used for prosthetics. To restore the integrity of the dentition, even distribution of masticatory load, appropriate prosthetics are performed. The use of the latest methods contributes to the effective treatment of periodontal disease in the early stages, stabilization of the process at deeper stages, in some cases helps to avoid surgery.

Clinical efficiency of ultraphonophoresis of Metrogyl Denta gel in mild periodontitis

BACKGROUND: Periodontal diseases are one of the most common in dentistry. Along with the complications of caries, periodontitis is one of the causes of tooth loss. In the etiology of this disease, the leading role is played by the microbial factor, as well as microcirculatory disorders in periodontal tissues. The complex mechanism of periodontitis development involves a comprehensive treatment approach aimed at different links in the pathogenesis of the development of this disease. Among the numerous physical factors used in the treatment of periodontal diseases, ultraphonophoresis, which combines the effects of ultrasound and a medicinal substance, is not widely used. The study of the effectiveness of the use of Metrogil Denta gel ultraphonophoresis in the treatment of inflammatory periodontal diseases is of considerable scientific and practical interest.
 AIM: This study aimed to investigate the effect of ultraphonophoresis of gel on the clinical course of chronic generalized periodontitis of moderate severity.
 MATERIALS AND METHODS: Sixty patients (aged 3162) with chronic generalized periodontitis of moderate severity were examined and treated. In the main group (n=30), treatment included high-frequency ultrasound and antibacterial preparation of Metrogyl Denta gel applied to the periodontal tissue (ultraphonophoresis of Metrogyl Denta gel). In the control group (n=30 patients), Metrogyl Denta gel was injected into the periodontal tissue.
 RESULTS: Application of Metrogyl Denta gel for moderate periodontitis did not improve local blood circulation. Six months after treatment, nearly half of the examined patients showed signs of inflammation, which was accompanied by a worsening of periodontal index (PI), SchillerPisarev tests results, and rheography finding. Twelve months after treatment, 63% of the patients developed complications.
 CONCLUSIONS: There was further deterioration in the clinical and functional parameters. After ultraphonophoresis of Metrogyl Denta gel, there was a decrease in the readings of the SchillerPisarev test, PI, and normalization of the qualitative and quantitative indicators of the rheoparodontogram, which indicated a decrease in inflammation and activation of local blood circulation in the periodontium. In the long term after treatment, despite the increase in hygiene index, the relative stability of clinical and functional parameters was maintained. Even a year after treatment, the level of indicators did not reach the level obtained before treatment.

The use of natural antioxidants in the treatment of inflammatory periodontal diseases

The use of natural antioxidants in the treatment of inflammatory periodontal diseases

Photodynamic therapy in the treatment of periodontal diseases

The article presents an overview of the use of photodynamic therapy in modern dentistry based on the literature sources. Various types of photosensitizers are presented, the action of which has a good effect on the course of the process in periodontal pockets. The conclusion is made about the vantages of using this method in the treatment of inflammatory periodontal diseases, as well as the effect of photodynamic therapy on the periodontal microbiota, which has a positive effect on the treatment of patients.

КЛИНИКО-ЛАБОРАТОРНАЯ ОЦЕНКА ПРИМЕНЕНИЯ БАЛЬЗАМА ДЛЯ ДЕСЕН «ЛЕСНОЙ БАЛЬЗАМ» ПРИ ЛЕЧЕНИИ ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЙ ПАРОДОНТА

Subject. Microorganisms are a fundamental factor in the development of inflammation. The choice of a medicinal composition in the form of a gel can provide a prolonged introduction of dosage forms into the focus of inflammation. For our research, the herbal medicine Gum balm "Forest Balm" was selected. The main components are aloe juice, bisabolol, panthenol and ginger extract, which have an anti-inflammatory and wound-healing effect; troxerutin, which helps reduce gum bleeding; fir extract, which blocks the growth of pathogenic oral microbiota.
 Purpose ― to assess the effectiveness of the use of gum balm "Forest Balsam" in the treatment of inflammatory periodontal diseases 
 Methodology. A survey of 30 volunteer patients aged 20 to 22 years was conducted. Dental examinations were performed at the following time intervals: before application, after the 1st application, after 3 days, after 5 days and 7 days of application. The prevalence of the inflammatory process was assessed using the papillary-marginal-alveolar index and the Muhlemann bleeding index (modified by Cowell). Qualitative and quantitative study of microbiological material was performed by measuring the buccal epithelial colonization index to determine the status of local immunity.
 Results. Changes in the cytological picture in the direction of normocenosis are determined on the third day of applying the gum balm "Forest balm". On the 7th day of application, it eliminates all visible symptoms of gum disease (bleeding, inflammation, swelling).
 Conclusions. Gum balm "Forest balm" improves the protective function of the oral cavity by activating cellular defense mechanisms, strengthens local immunity, activates its own immune defense of the oral cavity to fight opportunistic microorganisms, increases the colonization resistance of the oral mucosa to opportunistic microorganisms.

Analysis of the provision of paradontology in the Armed forces of the Russian Federation

A statistical study of the appeal to the dental clinic of S. M. Kirov Military Medical Academy patients who were diagnosed with chronic generalized periodontitis, as well as the analysis of treatment methods for inflammatory periodontal diseases in the Armed Forces based on data from the Main Military Medical Directorate for 2012-2018. Analysis of treatment methods for chronic generalized periodontitis in the military unit indicates the choice of a radical method - tooth extraction. Most patients who applied to the clinic of maxillofacial surgery and surgical dentistry were diagnosed with a severe degree of chronic generalized periodontitis. Basically, patchwork operations were performed on the upper jaw and lower jaw. The proportion of patients with moderate severity of chronic generalized periodontitis prevailed in the dental clinic. In the conditions of the dental clinic, no flap periodontal operations were performed, most often the method of curettage of periodontal pockets was used, which was carried out to improve the quality of the intervention according to classical recommendations in the field of several teeth, including using high-tech equipment. Timely and high-quality comprehensive treatment of inflammatory periodontal diseases is the basis for successful activities to reduce periodontal morbidity and improve oral health in military personnel. Due to the relevance of inflammatory periodontal diseases in the Armed Forces of the Russian Federation, the choice of treatment method should be based on etiological and pathogenetic therapy, dentists should abandon the radical method of tooth extraction.

Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Oxygenase-1 Pathway.

Human gingival fibroblast barrier dysfunction caused by inflammation contributes to gingivitis and can lead to inflammatory periodontal disease. The disease features include upregulated epithelial permeability, increased inflammatory mediators, and downregulated junctional complex molecules. Carbon monoxide- (CO-) releasing molecule-3 (CORM-3) is a water-soluble compound that has demonstrated anti-inflammatory effects in in vitro and in vivo studies. In this study, we aimed to investigate the effects of CORM-3 on the expression of tight and adherens junction molecules on human gingival fibroblasts (HGFs) stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). HGFs were cultured from the explants of normal human gingival tissues, which were stimulated in the presence or absence of CORM-3. Epithelial barrier function was evaluated by paracellular permeability and junctional complex molecule expression analyses. The protein and mRNA expression levels of adherens junction molecules (VE-cadherin and β-catenin) and tight junction molecules (zona occludens-1, ZO-1) were studied using western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-PCR). The mRNA and protein expression levels of these cytokines were also analyzed in HGFs transiently transfected with HO-1 small interfering RNA (siRNA) in response to TNF-α and IL-1β stimulation. CORM-3 reduced permeability and enhanced the expression of junctional complex molecules (ZO-1, VE-cadherin, and β-catenin) in TNF-α- and IL-1β-induced HGFs. However, these effects of CORM-3 were attenuated when HO-1 siRNA was transiently transfected in HGFs. These findings indicate that CORM-3 exerts anti-inflammatory effects on TNF-α- and IL-1β-stimulated HGFs via the HO-1 pathway, which suggests the promising potential of CORM-3 in the treatment of inflammatory periodontal disease.

Биофармацевтические исследования по выбору основы для стоматологического геля с экстрактом барбариса

Исследованы биофармацевтические свойства модельных гелевых композиций c экстрактом корня барбариса и использованием в качестве гелеобразователей метилцеллюлозы, натриевой соли карбоксиметилцеллюлозы, натриевой соли альгиновой кислоты и поливинилового спирта методами прямой диффузии в агаровый гель и диализа через полупроницаемую мембрану. Определены кинетические закономерности процесса и скорости набухания исследуемых гелевых основ. Показана целесообразность использования натриевой соли альгиновой кислоты для получения стоматологического геля с экстрактом корня барбариса, обладающего специфической бактериостатической активностью в отношении таких пародонтопатогенных микроорганизмов, как Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens и Actinobacillus actinomycetemcomitans, для профилактики и лечения воспалительных заболеваний пародонта.

Разработка новых подходов при лечении воспалительных заболеваний пародонта

Разработка новых подходов при лечении воспалительных заболеваний пародонта

Treatment of chronic periodontitis by non-surgical periodontal therapy in association with diode laser compared to conventional non-surgical therapy

Mechanical surface treatment and removal of the above and subgingival biofilm (Tartar ablation; SRP) are consideredthe most suitable tools for the treatment of periodontal inflammatory diseases, with the aim of destroying bacterialbioflim, reducing bacteria, and slowing down recolonization by pathogenic microorganisms.Often, however, the only S&RP are not enough, as there are patients who are experiencing relapses.Recently, laser therapy has been suggested as a potential tool to improve the outcome of periodontal non-surgicaltreatment.The objective of the following study was to evaluate the clinical healing of periodontal pockets treated with mechanical therapy, scaling and root planing, and diode laser application, compared to that obtained with non-surgical mechanical therapy alone.The study was designed as a randomized controlled clinical trial. Patients in the control group (13 patients) underwent conventional non-surgical therapy only, while patients in the test group (17 patients) were associated withconventional non-surgical treatment, a laser irradiation session.At baseline and after 6 months, the parameters of probing depth (PD), bleeding on probing (BOP), gingival recession(REC) were assessedThe main variable of this study was the PD (probing depth)FMPS and FMBS at follow-up improved in both groups. The FMPS baseline test group 32.59 ± 6.74 - follow up 12.00± 3.16.The baseline of the control group showed 33.00 ± 9.55, the follow up 13.15 ± 4.85 The FMBS baseline test groupfound 24.29 ± 5.01 while at follow 9.65 ± 2.69. The baseline control group 30.31 ± 7.74, Follow up 11.08 ± 2.33.There is a statistical significance. (P.VALUE 0.0001)There were no significant differences between the groups in terms of PD, CAL and BOP at baseline and at follow-up.PD Test group 4,89±1,58 3,95±0,85 0,0001Control group 5,02±1,57 4,01±0,86CAL (mm)Test group 0,89±2,29 0,77±1,91Control group 0,28±1,38 0,24±1,14REC Test group 0,19±0,49 0,19±0,51Control group 0,06±0,29 0,57±029BOP Test group 51,2% 23,5%Control group 54,0% 20,9%The results showed differences in both baseline and follow-up for REC.Test group 0,19±0,49 0,19±0,51Control group 0,06±0,29 0,57±029In intra-group analyzes, there are differences between baseline and follow-up for all values, except for REC in thecontrol group.The diode laser can be used as an appropriate device for periodontal treatments, but it can offer additional andsignificant benefits if used according to appropriate protocols and parameters, and especially if associated withnon-surgical, manual and ultrasound periodontal instrumentation, always site-specific , as it is a tool that does notreplace traditional methods.