Treatment Of Gastric Ulcer Research Articles

Abomasal ulcers: Do ranitidine or omeprazole prevent phenylbutazone-induced lesions in sheep?

Treatment and prevention of gastric ulcers are well known in monogastric animals; however, there are uncertainties about the preventive effects of acid-reducer drugs, their dosages and the best route of administration to ruminants. The object of this research was to test the preventive actions of ranitidine and omeprazole on abomasal ulcer development in sheep treated with a high dosage of non-steroidal anti-inflammatory drug. Eight healthy adult sheep with abomasal cannula were distributed in two contemporary and balanced 4 × 4 Latin squares (eight animals per group). For seven days, animals in the control group received high doses of phenylbutazone (4.4 mg/kg, every 12 h) while the treated groups received the same high doses of phenylbutazone plus an acid-reducer drug, namely 2 mg/kg of intravenous ranitidine every 12 h; 0.4 mg/kg of intravenous omeprazole; 4 mg/kg of oral paste omeprazole, both once a day. Intravenous omeprazole caused phlebitis, increasing serum fibrinogen content, and 75 % of the treated animals were diagnosed with type 1a ulcers in abomasal mucosa. Oral omeprazole showed no adverse effects, but was not effective in preventing type 1a ulcer in 50 % of animals, which was the same amount as diagnosed in control group. Despite of a minor heart rate increase, ranitidine treated animals showed only 37.5 % of abomasal injuries. Based on clinical findings, intravenous ranitidine is apparently better than omeprazole in prevention of abomasal ulcers, but another kind of experimental design comparing group by group is required and strongly recommended to ensure the beneficial effect of ranitidine.

Revealing the Active Compounds and Mechanism of Banxia Xiexin Decoction Against Gastric Ulcer by Network Pharmacology and Molecular Docking

Gastric ulcer (GU) is a clinically common gastrointestinal disease with a long disease course that frequently reoccurs. Banxia Xiexin decoction (BXD), a traditional Chinese medicine prescription, has a prominent protective effect against GU. Nonetheless, the therapeutic mechanisms of BXD against GU remain elusive. In this study, a rat model of GU was established by gavage with 95% ethanol, and BXD significantly attenuated the inflammatory effect of GU in rats. An “active ingredient–target” interaction and GU protein–protein interaction networks were constructed based on system biology, which could screen out the crucial active ingredients. The target protein–protein interaction network for the BXD treatment of GU was constructed to identify the key target proteins with network topology parameters. The DAVID database was then used to perform Gene Ontology and Kyoto encyclopedia of genes and genomes enrichment analysis on the proteins targeted by BXD in the treatment of GU. Finally, molecular docking technology was used to study the interactions between key active ingredients and core target proteins. A total of 89 active ingredients of BXD were screened and 63 target proteins of BXD in the treatment of GU were identified. Through the analysis of protein–protein interaction and the active ingredient–target protein network diagram, it was found that tumor necrosis factor-α(TNF-α), AKT1, and PTGS2 may play a key role in the treatment of GU by BXD. Molecular docking showed that these 3 core target proteins had a good affinity with the main components of BXD, including baicalein, norwogonin, and skullcapflavone II. The mechanism of BXD against GU may involve the inhibition of inflammatory response and oxidative stress, involving signaling pathways such as TNF, hypoxia-inducible factor-1, and mitogen-activated protein kinase. Network pharmacology and molecular docking technology indicated the key active ingredients, target proteins, and signal pathways that may be the biological basis of BXD in the treatment of GU.

RETRACTED: Corrigendum to "mPEG-b-P(Glu-co-Phe) nanoparticles increase gastric retention time and gastric ulcer treatment efficacy of 20(S)-ginsenoside Rg3" [Biomed. Pharmacother. 146 (2022) 112608

This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This correction has been retracted at the request of the Editors as it has been superseded by the retraction of the article it was intended to correct.

Comparison of Clinical Effects between Omeprazole and Omeprazole in the Treatment of Gastric Ulcer

Comparison of Clinical Effects between Omeprazole and Omeprazole in the Treatment of Gastric Ulcer

Clinical Study on the Kangfuxin Solution Combined with Omeprazole in the Treatment of Active Gastric Ulcer

Clinical Study on the Kangfuxin Solution Combined with Omeprazole in the Treatment of Active Gastric Ulcer

Chemical Investigation of Tetradium ruticarpum Fruits and Their Antibacterial Activity against Helicobacter pylori.

The fruit of Tetradium ruticarpum, known as Evodiae Fructus, is a traditional herbal medicine used to treat gastric and duodenal ulcers, vomiting, and diarrhea. The traditional usage can be potentially associated with the antibacterial activity of T. ruticarpum fruits against Helicobacter pylori. However, so far, the antibacterial activity of T. ruticarpum fruits and antibacterial components against H. pylori has not been investigated despite the traditional folk use. The current study was conducted to investigate the bioactive chemical components of T. ruticarpum fruits and evaluate their antibacterial activity against H. pylori. Phytochemical investigation of the EtOH extract of T. ruticarpum fruits led to the isolation and identification of nine compounds (1–9), including phellolactone (1), the absolute configuration of which has not yet been determined. The chemical structures of the isolated compounds were elucidated by analyzing the spectroscopic data from one-dimensional (1D) and two-dimensional (2D) NMR and high-resolution electrospray ionization mass spectrometry (HR-ESIMS) experiments. Specifically, the absolute configuration of compound 1 was established by the application of computational methods, including electronic circular dichroism (ECD) calculation and the NOE/ROE-based interproton distance measurement technique via peak amplitude normalization for the improved cross-relaxation (PANIC) method. In the anti-H. pylori activity test, compound 3 showed the most potent antibacterial activity against H. pylori strain 51, with 94.4% inhibition (MIC50 and MIC90 values of 22 and 50 μM, respectively), comparable to that of metronidazole (97.0% inhibition, and MIC50 and MIC90 values of 17 and 46 μM, respectively). Moreover, compound 5 exhibited moderate antibacterial activity against H. pylori strain 51, with 58.6% inhibition (MIC50 value of 99 μM), which was higher than that of quercetin (34.4% inhibition) as a positive control. Based on the bioactivity results, we also analyzed the structure–activity relationship of the anti-H. pylori activity. Conclusion: These findings demonstrated that T. ruticarpum fruits had antibacterial activity against H. pylori and could be used in the treatment of gastric and duodenal ulcers. Meanwhile, the active compound, 1-methyl-2-(8E)-8-tridecenyl-4(1H)-quinolinone (3), identified herein also indicated the potential application in the development of novel antibiotics against H. pylori.

A Comparative Study on Shengyang Yiwei Decoction Combined with Rebaparte in the Treatment of Gastric Ulcer of Spleen and Stomach Qi Deficiency and Dampness Stagnation with Middle-jiao Type

A Comparative Study on Shengyang Yiwei Decoction Combined with Rebaparte in the Treatment of Gastric Ulcer of Spleen and Stomach Qi Deficiency and Dampness Stagnation with Middle-jiao Type

Protective effect of active components of Eucommia ulmoides leaves on gastric ulcers in rats: Involvement of the PI3K/Akt/NF-κB pathway.

Eucommia ulmoides leaves are widely developed as food and medicines in China and Japan. Its main components have anti-inflammatory properties against gastric ulcers. The purpose of this study was to assess the protective role of an extract derived from the active components of Eucommia ulmoides leaves (EUL 50) against a gastric ulcer and analyze the underlying antiulcer mechanism. The main components of EUL 50 were identified using an ultra-performance liquid chromatography (UPLC) method. Network pharmacology and molecular docking were performed to predict the possible mechanism of action of EUL 50 in the treatment of gastric ulcers. The rats received EUL 50 intragastric administration twice a day for 3 days. Hydrochloric acid/ethanol (HCl/EtOH) was utilized to induce gastric ulcers, followed by histopathological and histochemical evaluation of the ulcer tissues and determination of the main oxidative stress parameters and inflammatory cytokines. The expression of PI3K/Akt/NF-κB pathway-related proteins was measured. Neochlorogenic acid, chlorogenic acid, rutin, and so on were identified as the major components of EUL 50 by UPLC. The prediction results identified the PI3K/Akt/NF-κB signaling pathway as the main possible protective mechanism against gastric ulcers. Furthermore, in a dose-dependent manner, EUL 50 reduced gastric tissue damage. In addition, the high dose of EUL 50 administration resulted in remarkable reductions in the levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin-1β (IL-1β) by 22.64%, 42.61%, 57.78%, and 56.51%, respectively, and suppression of the phosphorylation of Akt, p65, IKKα, and IκBα by 60.87%, 67.65, 74.58%, and 59.57%, respectively, and increased the antioxidant enzyme activity. EUL 50 is rich in flavonoids and organic acids that can act on the PI3K/Akt/NF-κB signaling pathway; as a result, oxidative stress and inflammation are considerably reduced, and gastric ulcers caused by HCl/EtOH are reduced. PRACTICAL APPLICATION: As a medicinal and food substance, Eucommia ulmoides leaves are widely used in the development of health products. EUL 50, a moderately polar part of E. ulmoides leaves, was obtained by extraction and enrichment and was found to have a better protective effect against HCl/EtOH-induced gastric ulcers. This finding can enrich the traditional application of E. ulmoides leaves and provide a basis for their health product development.

Clinical Analysis of Drug Treatment of Gastric and Duodenal Ulcer in the Department of Gastroenterology

Clinical Analysis of Drug Treatment of Gastric and Duodenal Ulcer in the Department of Gastroenterology

Antisecretory and Spasmolytic Activities of Aqueous and Ethanolic Stem Bark Extracts of Nauclea diderrichii in Wistar Rats.

Background Diarrheal diseases are a major cause of morbidity and mortality throughout the world and particularly in developing countries. Nauclea diderrichii is a plant used in traditional medicine in the treatment of anemia, fever, gastric ulcer, malaria, abdominal pain, skin infections, and diarrhea. The present work is aimed at evaluating the antisecretory and spasmolytic activities of aqueous and ethanolic stem bark extracts of Nauclea diderrichii in Wistar rats. Methods The effect of aqueous and ethanolic extracts of Nauclea diderrichii was tested at doses of 100, 200, and 300 mg/kg on castor oil-induced secretory diarrhea, misoprostol-induced fluid accumulation, and the effect of pretreatment with yohimbine and glibenclamide. They were also tested on normal motility and castor oil- and carbachol-induced hypermotility. Results The results showed that the aqueous and ethanolic extracts of Nauclea diderrichii significantly (p < 0.001) inhibited castor oil-induced secretory diarrhea at all the doses. Both extracts significantly (p < 0.001) inhibit fluid accumulation induced by misoprostol. The pretreatment with glibenclamide reduced the antidiarrheal activity of aqueous extract of Nauclea diderrichii. The pretreatment with yohimbine did not alter the effect of the aqueous extract of Nauclea diderrichii. On intestine transit as on castor oil- and carbachol-induced motility, the aqueous and ethanolic extracts at doses of 100 and 200 mg/kg reduced significantly (p < 0.05, p < 0.01, and p < 0.001) the travelled distance by charcoal and peristaltic index. Conclusions The study demonstrated that the aqueous and ethanolic extracts of Nauclea diderrichii possess antisecretory and antispasmolytic properties hence its use in traditional medicine against diarrhea.

Biocompatible, adhesive and stable GelMAc/PVAMA/MPDA@Cur hydrogels regulate immune response to improve endoscopic submucosal dissection-induced gastric ulcer healing in vivo

Endoscopic submucosal dissection (ESD) is one of the most important strategies for early gastrointestinal tumor treatment. However, the ideal healing of artificial ulcers produced after ESD procedure are still hard to achieve. In a gastric ulcer environment, the inherent peristole, acid condition and excessive oxidative stress seriously affect the therapeutic outcome of traditional medication. Curcumin (Cur) is a promising anti-inflammatory drug for gastric ulcer treatment, but its long-term use is still challenging. A polymer-assisted drug delivery system in gastrointestinal disease treatment is a new direction but is still at an initial stage. Herein, we report a hybrid hydrogel consisting of catechol motif-modified methacrylated gelatin (GelMAc) and methacrylated poly (vinyl alcohol) (PVAMA). GelMAc brought significant adhesive property and biocompatibility. PVAMA could effectively provide stability and mechanical strength. Moreover, mesoporous polydopamine nanoparticles were prepared as the drug carrier to load Cur via π-π stacking and hydrogen bonding interactions, and they were encapsulated in the hybrid GelMAc/PVAMA hydrogel. The prepared sample was proved to have a unique stability on gastric tissue even bearing acid (pH 2) and oxidative stress (H2O2) conditions. The slowly released Cur from the hydrogel was capable of inducing M2 polarization of macrophages. In an in vivo gastric ulcer model, the prepared sample remarkably improved gastric ulcer healing by curbing the pro-inflammatory response and alleviating oxidative stress. An accelerated reepithelization and angiogenesis were also observed. Thus, this study provides a promising hydrogel for the treatment of ESD-induced ulcers, which may attract more attentions to explore the deeper combination between biomaterials and gastrointestinal research.

Actualization of the modern approach to the treatment of gastric ulcer

Despite the generally accepted fact that Helicobacter pylori is the main cause of gastric and duodenal ulcers, its role is not always decisive. It was found that this pathogen does not have a sufficient level of invasiveness, and in about 70% of cases does not lead to morphological disorders of the gastric and intestinal mucosa. Speaking about the possible causes of the development of peptic ulcer, one cannot but take into account the influence of stressful situations, aggravated heredity, and nutritional errors. One of the urgent problems of modern medicine is to increase the effectiveness of peptic ulcer treatment and prevent recurrence of the disease, and the main direction of antiulcer therapy is the eradication of Helicobacter pylori with the parallel administration of antisecretory drugs. Meanwhile, it should be noted that the lack of certainty in matters of pathogenesis over a number of years led to the unreasonable prescription of a large number of various medications, ranging from antacids and antispasmodics to sedatives and tranquilizers. Such a wide variety of therapeutic drugs used indicates that for a long time it was not possible to confirm the real effectiveness and clinical efficacy of most antiulcer drugs, which once again confirmed the correctness of the phrase said by the famous therapist V.Kh. Vasilenko regarding the fact that "gastroduodenal ulcers heal due to treatment, despite treatment and even in spite of treatment".

Mechanism of Saikosaponins from Radix bupleuri in the Treatment of Acetic Acid-Induced Gastric Ulcer in Rats

Mechanism of Saikosaponins from Radix bupleuri in the Treatment of Acetic Acid-Induced Gastric Ulcer in Rats

Ilex rotunda Thunb Protects Against Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Restoring the Intestinal Mucosal Barrier and Modulating the Oncostatin M/Oncostatin M Receptor Pathway.

Ilex rotunda Thunb (IR) is a traditional Chinese medicine used for the clinical treatment of gastric ulcers and duodenal ulcers; however, the effect of IR on ulcerative colitis (UC) and its underlying mechanism remains unclear. This study investigated the therapeutic effect of IR on UC mice induced by dextran sulfate sodium (DSS) as well as the potential underlying mechanism. The main components of IR were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Then we established a model of UC mice by administering 2.0% DSS for 7 days followed by 2 weeks of tap water for three cycles and administered IR. On day 56, the disease activity index (DAI), colon length, pathological changes, and inflammatory response of the colon tissue of mice were assessed. The oxidative stress and apoptosis of colon tissue were detected, and the integrity of the intestinal mucosal barrier was evaluated to assess the effect of IR. Furthermore, the relationship between oncostatin M (OSM) and its receptor (OSMR) in addition to the IR treatment of UC were evaluated using a mouse model and Caco2 cell model. The results showed that IR significantly alleviated the symptoms of UC including rescuing the shortened colon length; reducing DAI scores, serum myeloperoxidase and lipopolysaccharide levels, pathological damage, inflammatory cell infiltration and mRNA levels of interleukin one beta, tumor necrosis factor alpha, and interleukin six in colon tissue; alleviating oxidative stress and apoptosis by decreasing kelch-like ECH-associated protein 1 expression and increasing nuclear factor-erythroid factor 2-related factor 2 and heme oxygenase-1 protein expression; and promoting the regeneration of epithelial cells. IR also promoted the restoration of the intestinal mucosal barrier and modulated the OSM/OSMR pathway to alleviate UC. It was found that IR exerted therapeutic effects on UC by restoring the intestinal mucosal barrier and regulating the OSM/OSMR pathway.

Cellulose-Conjugated Copper-Oxide Nanoparticles for the Treatment of Ethanol-Induced Gastric Ulcers in Wistar Rats.

Gastric ulcer (GU) is the most common and chronic inflammatory condition mediated by multiple immune cells like neutrophils, macrophages, and lymphocytes with multiple pro-inflammatory cytokine interleukins such as IL-8, IL-10, IL-β, and interferon-γ (IFN-γ). Copper (Cu) is one of the essential micronutrients mainly found in the liver and brain. It plays a major role in metabolism, enzyme conversion, free radical scavenging, trafficking agents, and many others. Due to its various roles in the biological system, it can also be used as a therapeutic agent in many diseases like colon cancer, bone fracture healing, angiogenesis, as an antibacterial, wound-healing and radiotherapeutic agents. In this study, we used thiol-functionalized cellulose-conjugated copper-oxide nanoparticles (CuI/IIO NPs) synthesized under environmentally friendly conditions. We have evaluated the effects of cellulose-conjugated CuI/IIO NPs against ethanol-induced gastric ulcer in Wistar rats. The cellulose-conjugated CuI/IIO NPs were evaluated against different physical, histochemical, and inflammatory parameters. The NPs promoted mucosal healing by ameliorating ulcerative damage, restoring the histoarchitecture of gastric mucosa, and inhibiting pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), and other inflammatory biomarkers such as myeloperoxidase (MPO) activity and nitric oxide (NO) levels. The current study's findings suggest that cellulose-conjugated CuI/IIO NPs exerted antiulcer effects on the preclinical rat model and have promising potential as a novel therapeutic agent for the treatment of gastric ulcers.

Network Pharmacology Analysis and Experimental Verification Strategies Reveal the Action Mechanism of Danshen Decoction in Treating Ischemic Cardiomyopathy.

Background Danshen Decoction comprises Salvia miltiorrhiza, Santalum album, and Amomum villosum. It can promote blood circulation and remove blood stasis, and is commonly used in the treatment of gastric and duodenal ulcers, coronary heart disease, angina pectoris, etc. This research is based on network pharmacology and is experimentally verified to explore the potential mechanism of Danshen Decoction in the treatment of ischemic cardiomyopathy (ICM). Methods The effective components and targets of Danshen Decoction were firstly extracted from Traditional Chinese Medicine Systems Pharmacology (TCMSP) Database and Analysis Platform, the drug-component-target-disease network was then constructed, the protein-protein interaction (PPI) network was constructed, the Gene Ontology (GO) enrichment analysis was carried out, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was analyzed in order to find the potential active components and therapeutic mechanisms. Finally, the in vitro hypoxia/reoxygenation model in H9c2 cells was established to verify the predicted active components and therapeutic mechanisms. Results The results showed that Danshen Decoction has 67 potential active components and 109 therapeutic targets in treating ICM. These targets were rich in a variety of gene functions and different signaling pathways; the main gene targets include TP53, c-Jun, and Akt1. Go enrichment analysis showed that response to drug, membrane raft, and G protein-coupled amine receiver activity rank first in each process, and the main signaling pathways include PI3K-Akt signaling pathway. Through molecular docking and experimental verification of the major active components and core therapeutic targets, the active components of Danshen Decoction demonstrated an ability to reduce the cell damage caused by hypoxia/reoxygenation in H9c2 cells by regulating the core therapeutic target including Akt1, c-Jun, and TP53. Conclusion Danshen Decoction has the effect of treating ICM in multiple ways, which is consistent with the results of network pharmacology. This laid a foundation for further study in exploring the active principles and pharmacological mechanism of Danshen Decoction.

Antiulcer effect of Helminthostachys zeylanica (L.) Hook. root extract on ethanol-induced acute gastric lesion in Wistar rats

Context: Helminthostachys zeylanica is well known as a traditional medicine with various pharmacological activities, including antioxidant and anti-inflammatory. These activities are needed in the treatment of gastric ulcers. Aims: To evaluate the effectiveness of H. zeylanica as an antiulcer agent on acute peptic ulcer. Methods: The antiulcer effect of the H. zeylanica ethanolic extract (100, 200 and 400 mg/kg BW) was studied through ulcer index, physicochemical properties of gastric juice and histopathological analysis in male Wistar rats. Results: The extract reduces ulcer index, gastric fluid volume, total acidity and increases gastric protection factors such as pH and mucus levels. These results were not significantly different from omeprazole (p&gt;0.05). Photomicrograph analysis showed improvement of membrane structure in extract-treated animals. Conclusions: H. zeylanica extract could be valued as an antiulcer agent.

Genus mallotus (euphorbiaceae): a review on traditional medicinal use, phytochemistry and biological activities

Ethnopharmacological relevance: The genus Mallotus (Euphorbiaceae family) includes 124 accepted names and distributes in tropical and sub-tropical regions. Some Mallotus species are used in traditional medicines for the treatment of chronic hepatitis, enteritis stomach, disorders, cholelithiasis, anti-cancer, gastric, and duodenal ulcers.&#x0D; Aim of the study: According to the Scifinder database, this is the first review study that focuses on the phytochemistry and pharmacology of Mallotus genus (17 Mallotus species) to understand the link between the traditional medicinal uses, phytochemistry, and bioactivities. Thus, they provides a scientific fundamental for further research in the phytochemical and pharmacological activities of their species.&#x0D; Materials and methods: Information about the Mallotus genus was collected using various databases, such as Web of Science, SciFinder, PubMed, Sci-hub, Google Scholar, Wiley, Elsevier, ACS publications, and SpringerLink between 1978 and 2021. Plant names were validated by “The Plant List” (www.theplantlist.org).&#x0D; Results: Up to now, total 325 compounds were reported from Mallotus species, including phloroglucinols, steroids, coumarins, benzopyrans, flavonoids, chalcones, galics, and bergenins. The extracts and phytochemical constituents of the Mallotus genus are a rich source of biologically active, including anti-cancer, anti-inflammatory, immunoregulatory, antioxidant, antibacterial, antifungal, anti-virus, and other activities.&#x0D; Conclusions: The review indicated that the Mallotus genus is a promising source of biological activities compounds, special anti-cancer chromanes. The results of this review confirm the great potential of Mallotus species. Thus, it will be helpful for further research in the phytochemistry and pharmacology of the Mallotus genus.

Exploration on the Efficacy of Rebeprazole Sodium Enteric-soluble Tablets and Omeprazole in Gastric Ulcer Treatment

Exploration on the Efficacy of Rebeprazole Sodium Enteric-soluble Tablets and Omeprazole in Gastric Ulcer Treatment

Scrophularia striata: Therapeutic and Healing Properties with an Emphasis on Oxidative Stress and Gastric Ulcer Treatment

: Some plants, including Scrophularia striata, have traditionally been used among people of the Zagros region for infection and wound healing. Therefore, the purpose of this research was to investigate the therapeutic and healing effects of S. striata with an emphasis on oxidative stress and gastric ulcer treatment. Antirrhinum majus, with a local name of S. striata, is a wildling and perennial plant from the A. majus family, found in most temperate and tropical regions of Iran, including Ilam. Substances, including cinnamic acid, quercetin, isorhamnetin-3-O-rutinoside, nepitrine, phenylpropanoid glycoside (osteoside 1), alcohol allyl, and D-N-octyl phthalate, have been identified in S. striata. People living in Ilam province have been using this plant experimentally for many years in various forms such as edible decoction, incense, and poultice in the treatment of various diseases such as inflammation and infection of the eyes and ears, skin burns, infectious wounds, episiotomy, pain, gastrointestinal disorders, colds, hemorrhoids, and boils. The S. striata extract has a significant positive effect on the size and number of gastric ulcers, and with increasing the concentration of the extract, the size and number of the wounds decrease. In general, the present study showed that the S. striata medicinal plant has a significant restorative effect on skin and gastrointestinal wounds, especially gastric ulcers, but clinical trials are required for the oral and therapeutic use of S. striata.