Treated Sugarcane Bagasse Research Articles

Analisis Pengaruh Perlakuan Microwave Plasma dalam Cairan Terhadap Kadar Glukosa dan Produk Nano Material Pada Ampas Tebu

Sugarcane (Saccharum officinarum L.) is a raw material that is processed to produce crystal sugar and bioethanol. The process produces sugarcane bagasse. In this study, The liquid-plasma treatment based on microwave plasma in the liquid was used to treat sugarcane bagasse to observe the effect on glucose concentration and gain byproducts. The energy input parameters were varied by 340, 360, and 400 W with irradiation time variations of 1, 3, and 5 minutes. Nelson Somogyi's method was used to determine the percentage of glucose as well as Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), and Image-J software to characterize the side-product of the samples. The results showed that the glucose concentration reduced around 27.6% - 99.2 % after microwave plasma in-liquid treatment at the energy input of 340 Watt and 360 Watt, respectively, with an irradiation time of 5 minutes. The side-product is cellulose nanoparticles which have an average diameter of 92, 106, and 169 nm

Decontamination of Zinc, Lead and Nickel from Aqueous Media by Untreated and Chemically Treated Sugarcane Bagasse: A Comparative Study

A methodology treatment was developed to removethe toxic metals suchas Zn(II), Pb(II) and Ni(II) from its aqueous solution using sugarcane bagasse (SB). In this study, Variety parameters like pH, contact time, adsorbent dose and the initial metal ions concentration were studied in batch experiments. The maximum removal capacities for Zn(II) were 92% for untreated SB and 96% for treated SB with H2SO4, for Pb(II) were found 86% for untreated SB and 92% for treated SB with H2SO4 while for Ni(II) were found 78% for untreated SB and 90% for treated SB with H2SO4 at optimum conditions. The maximum removal of Zn(II), Pb(II) and Ni(II) was obtained at 5 ppm initial concentration. It was noted that the metal ions removal capacity of this adsorbent was SB-H2SO4> SB-native, which indicated that the chemical treatment of SB enhanced the biosorption of metal ions. To describe the adsorption isotherms, langmuir and freundlich models were used. The Langmuir isotherm was found to be in a good agreement with experimental data of R2 value equal to 0.99 while R2 value at the range (0.91-0.97) for Freundlich isotherm. The biosorption process obeyed pseudo-second-order kinetics. This study showed that SB is a suitable and low cost-effective adsorbent for Zn(II), Pb(II) and Ni(II) ions removal of from their aqueous solutions.

Production of enzymatic complex from agro-industrial biomass and its application in combustible ethanol

Waste biomass and agro-industrial by-products, for production ethanol, will meet much of the great demand for this product. To reduce costs and optimize production, this study investigated solid-state fermentation (SSF) to obtain crude enzyme complex (CEC) from different agro-industrial biomasses (sugarcane bagasse, corn peel bran, rice straw bran and roasting and ground coffee residue) using cellulolytic fungi. The most promising CEC were evaluated in simultaneous hydrolysis and fermentation (SHF) for ethanol production by Saccharomyces cerevisiae in a culture broth containing sugarcane bagasse treated by steam explosion, and roast and ground coffee residue. In SSF with bioreactor volume of 0.25 L, containing 40 g of the biomass mixture and 40 g of sterile water with resuspended cells (1.0 x108 spores/g of solid medium) and temperature of 30±2 ºC, the strains Trichoderma reesei and Penicilium oxalicum provided the best enzyme activity. The CEC of T. reesei provided a concentration of 7.5 g L-1 of ethanol in a substrate containing treated sugarcane bagasse (60%) and roast and ground coffee residue (40%), under SHF conditions (pH 4.5, 35±2 °C, 48 h). The results obtained in this study show a promising alternative for correct disposal and use of residues and agro-industrial by-products by use in the production of enzymes and lignocellulosic ethanol.

Evaluating the Effect of Residual Lignin Contents and Fermentation Systems on Bioethanol Production

Peroxide-acetic acid (Peroxide-HAc) pretreatment is a simplified and cost-effective technology due to its mild conditions without the catalysis of strong acids, which was introduced to treat sugarcane bagasse (SCB) in this paper. Then pretreated SCB was used to produce bioethanol using thermophilic Saccharomyces cerevisiae in deionized water (DW) and sodium citrate buffer solution (SCS) fermentation systems, respectively. Results: showed that peroxide-HAc pretreatment can selectively remove lignin and change morphological structures, thus positively increasing saccharification and fermentation efficiency downstream. SCB pretreated at 100 °C obtained the lowest residual lignin content of 2.88% and achieved the highest ethanol yield of 92.10% (0.45 g/g-biomass) in DW system. In particular, SCS can decrease the residual sugars content of pretreated SCB during simultaneous saccharification and co-fermentation (SSCF) process. The addition of Tween 80 improved SSCF of SCB pretreated at 80 °C likely because Tween 80 can block residual lignin adsorption with enzymes. The concentration of acetic acid and glycerol byproducts from SCB pretreated at 80 °C can be decreased by SCS and DW systems, respectively, comparing to SCS and DW systems with additing Tween 80. This study provided a systematic process route for the bioethanol production by mild pretreatment method.

Determination of Mechanical Properties and Characterization of Alkali Treated Sugarcane Bagasse, Pine Apple Leaf and Sisal Fibers Reinforced Hybrid Polyester Composites for Various Applications

In this work tensile, flexural, compression, impact and hardness properties of alkaline treated sugarcane bagasse (SB), pine apple leaf (PALF) and sisal fiber (SF) reinforced with polyester composites are expressed for the first time. This effort to develop hybrid composite materials with improved execution for worldwide applications is a progressing procedure. Be that as it may, with their remarkable and wide scope of inconstancy, common fiber composites could rise as another elective designing material which can substitute the utilization of manufactured fiber composites. The challenge in working with natural fiber composites (NFC) is the large variation in properties and characteristics. In this experimental investigation SF as a base material, PALF and SB are filler materials. The weight percentage of SF has been maintained as constant and the remaining two fiber fillers were varied. The NFC are prepared by hand layup method and mechanical characteristics of tensile, flexural, compression, impact and hardness tests were performed as per ASTM standard. The experiment results revealed a consistent tendency of an increase in the above mechanical attributes to adding natural fibers/fillers. Better interlinking capability between the NFC and polyester matrix is also responsible for the effective resistance capability. This type of composite materials can be useful for various engineering fields.

Mehanske lastnosti in absorbcija vode izbranih naravnih vlaken kot zamenjava za azbest

This work investigates the influence of banana (Musa Sapientum) and jute fibers as a reinforcement in a cement/waste brown paper pulp matrix for applications in housing construction in Nigeria. The natural fibers were extracted from banana trunk and cocoa sack and, thereafter, treated with 1-M sodium hydroxide (NaOH) for 2 h to expose the lignin and cellulose and thus expose the hemicellulose content of the fibers. The treated fibers were allowed to dry in air for 7 days before cutting into short fibers of 10 mm length. Paper pulp was prepared by soaking waste brown cardboard papers in water for 24 h, thereafter, it was grinded in the paper pulp machine to form a paper pulp slurry, and then sun-dried for 5 days. The treated fibers were thoroughly mixed with the dried paper pulp to develop Fiber Cement Board (FCB) samples of varied weight content (5, 10, 15, and 20) w/% using the hand lay-up technique with the aid of a cold compacting machine. Thereafter, an appropriate quantity of cement as binder and treated sugarcane bagasse as filler material were added to the mix. The developed FCB samples were allowed to cure in air in the laboratory for 28 days before testing. Flexural, compressive, thermal conductivity, and water absorption tests were carried out on the samples using a universal testing machine, Lee's disk apparatus, and the percentage weight of different immersed samples in water, respectively. The results of the mechanical properties examined showed that FCB sample D containing (10 w/% banana fiber, 10 w/% jute fiber, 15 w/% cement/bagasse and 65 w/% paper pulp) gave the optimum results for the flexural and compressive properties with a respective value of 0.843 MPa and 7.333 MPa, while FCB sample F gave the best results for the thermal conductivity and water-absorption property.

Cetylpyridinium bromide (CPB)-treated sugarcane bagasse for the removal of chromate in aqueous solution

Modification of sugarcane bagasse (SB) by cetylpyridinium bromide (CPB) creating surfactant-modified SB (SBC) for the removal of chromate has been studied. The CPB adsorbed increased in line with the increased CPB concentrations, and infrared spectroscopy showed preserved SB structure after modification. SB showed little adsorption capacity in the adsorption study; however, the adsorption of chromate is proportional to the amount of CPB adsorbed on SB, possibly due to the attached CPB molecules on the SB. CPB molecules create a bilayer formation on SB, generating anion exchange sites for chromate to be adsorbed on SBC. The adsorption behavior of chromate on SBC followed the Langmuir equation model better than the Freundlich model. A better adsorption capacity was noted at the lower pH value of the chromate solution. In conclusion, surfactant-modified SB could be a potential alternative adsorbent for removing toxic chromate anion in water.

Development of One Pot Strategy for Hyper Production and In Vivo Evaluation of Lovastatin.

The aim of this project was to improve the Aspergillus terreus strain and pretreatment of sugarcane bagasse as carrier substrate for bulk production of lovastatin, a cholesterol-lowering drug, in solid state fermentation. Sugarcane bagasse was treated with alkali (1–3% NaOH) for the conversion of complex polysaccharides into simple sugars for better utilization of carrier substrate by microorganism for maximum lovastatin production. Ethidium bromide (time of exposure 30–180 min) was used to induce mutation in Aspergillus terreus and the best mutant was selected on the basis of inhibition zone appeared on petri plates. Fermented lovastatin was quantified by high-performance liquid chromatography. The fermented lovastatin, produced by parent and mutant Aspergillus terreus strain, was checked on body weight, blood glucose and serum cholesterol, ALT, AST, HDL-C, LDL-C, TG and TC levels of rats for their cholesterol lowering capacity. Our results indicate that selected strain along with 2% NaOH treated sugar cane bagasse was best suitable for bulk production of lovastatin by fermentation and fermented lovastatin effectively lower the cholesterol level of rats.

Removal of hexavalent chromium concentration from the aqueous solution using chemically treated sugarcane bagasse powder

Removal of hexavalent chromium concentration from the aqueous solution using chemically treated sugarcane bagasse powder

Treatment of malachite green dye containing solution using bio-degradable Sodium alginate/NaOH treated activated sugarcane baggsse charcoal beads: Batch, optimization using response surface methodology and continuous fixed bed column study

In this study, carbonized material was produced using sodium hydroxide treated Sugar cane bagasse (SB), and synthesized materials ware used to prepare Sodium Alginate/SBAC composite beads which were further used as an adsorbent to remove malachite green dye (MG) present in water. Physiochemical characteristics of composite beads were analyzed using FTIR, SEM, TGA, and BET. Adsorption equilibrium data showed excellent fit to the Freundlich model (R2 = 0.994) than to the Langmuir model (R2 = 0.925). Adsorption kinetics study indicated that the MG removal process would be better described by the pseudo-second-order kinetic model. The thermodynamic study suggested the spontaneous and endothermic nature of MG adsorption. By using response surface methodology, the optimum conditions for MG adsorption on composite beads were found to be 115.43 min, 0.3 g/L and pH 8 for contact time, adsorbent mass, and pH respectively and MG adsorption efficiency was 97.88%. The fixed-bed column data were evaluated using several kinetic models and among them, Thomas model showed the best agreement with investigation results. These results revealed that synthesized composite beads have a high affinity toward MG and it could be reasonable, eco-friendly adsorbent for dye removal from wastewater.

Effect of lignin isolated from p-toluenesulfonic acid pretreatment liquid of sugarcane bagasse on enzymatic hydrolysis of cellulose and cellulase adsorption

During lignocellulosic enzymatic hydrolysis, lignin is generally considered negative. To investigate the relation between lignin profiles and the effect of lignin on hydrolysis, in this study, the enzymatic hydrolysis efficiency and cellulase adsorption amount of Avicel and PB (p-toluenesulfonic acid (p-TsOH) treated sugarcane bagasse) were firstly determined before and after adding the p-TsOH lignin (isolated lignin from PB). Subsequently, the particle size, crystallinity, hydrogen bonds, electrostatic charges and hydrophobicity of PB and Avicel, and the chemical bonds of p-TsOH lignin were measured. The results indicated that adding the p-TsOH lignin inhibited Avicel’s hydrolysis, but increased the cellulase adsorption. This might be because after adding p-TsOH lignin, the lignin adsorbed more cellulase and the cellulase molecules on Avicel decreased. For PB, adding the p-TsOH lignin reduced the cellulase adsorption, but did not impact the hydrolysis efficiency. This might be due to the decrease in non-productive adsorption of cellulase.

The Strength of Polylactic Acid Composites Reinforced with Sugarcane Bagasse and Rice Husk

There are more than 1000 species of cellulose plants available in fiber form. A number of them are by-products from the major food crops contain lignocellulosic sources and being investigated as composite reinforcement materials. Sugarcane bagasse and rice husk are potential reinforcement materials and they were used to reinforce polylactic acid (PLA) matrix to make green composites. In this research work, sugarcane bagasse was given two different kinds of treatment; some were alkali treated using 8 wt.% NaOH at room temperature for an hour and some other were steam treated at 0.75 MPa for 30 minutes. The fiber content of the composites changed with weight percentage ratios of sugarcane bagasse/rice husk/PLA was 25/0/75, 25/5/70 and 25/10/65. Flexural strength was tested in accordance with ASTM D790-17 and structural evaluation was evaluated using scanning electron microscope (SEM) on the fracture section of the flexural test samples. Composites produced using steam treated sugarcane bagasse and rice husk have lower area density (1277-1385 g/m2) compared to the ones formed using NaOH treated bagasse and rice husk (1162-1500 g/m2). Both values of area density are below the density of neat PP and wood flour reinforced PP/PE composites used as reference materials. The flexural test shows the NaOH treatment on the bagasse fibers improve the flexural strength of the composites but the rice husk content introduced to the structure reduces the strength of the composites. SEM evaluation shows fiber fracture and few pull-out.

Enhanced hydrolysis of hydrothermally and autohydrolytically treated sugarcane bagasse and understanding the structural changes leading to improved saccharification

The enzymatic mixture containing Novozyme Cellic CTec2 spiked with xylanase derived from Thermomyces lanuginosus (1000 U xylanase per g of substrate) resulted in enhanced saccharification of native, hydrothermally and autohydrolytically pretreated sugarcane bagasse when compared to the benchmark Cellic CTec2. Hydrothermally pretreated bagasse upon hydrolysis with the enzymatic mixture yielded considerably higher levels of sugars as compared to benchmark Cellic CTec2, resulting in saccharification efficiencies of 85.7, 77.8 and 78.9% at 10, 15 and 20% (weight per volume) substrate loadings, respectively. Autohydrolysed and native sugarcane bagasse samples had lower saccharification yields, although xylanase addition to the benchmark cocktail still was beneficial. Chemical compositional analysis and detailed morphological examination of the substrates after pretreatments as well as the enzymatic hydrolysis reactions employing confocal laser scanning microscopy (CLSM), field emission scanning electron microscopy (FESEM), X-Ray diffraction (XRD) and solid state nuclear magnetic resonance (ssNMR) provided fundamental insights into the structural changes during deconstruction and shed light on how such changes affected the biomass enzyme hydrolysis.

Alkali-peroxide treatment of sugar cane bagasse. Effect of chemical charges on the efficiency of xylan isolation and susceptibility of bagasse to saccharification

Sugar cane is a promising raw material for the extraction of hemicelluloses. An alkali treatment of sugar cane bagasse under proper conditions followed by a precipitation using a reasonable quantity of ethanol can be an effective method to isolate these polysaccharides. In this work, bagasse is treated to obtain two products: (a) polymeric hemicellulose and (b) an enzymatic hydrolysate from the treated bagasse after hemicellulose extraction. The effects of charges of sodium hydroxide (10, 20, 40% w/w) and hydrogen peroxide (0, 10, 20% w/w) in the alkali treatment were evaluated. A 32 experimental design was considered under control of metal ions and inert atmosphere during alkaline treatment. An acceptable proportion of xylose plus arabinose could be extracted from bagasse (up to 18.4 g/100 g of bagasse). Both the alkali and peroxide showed strong effects on the yield of the extraction, as well as on the yield of the precipitation in ethanol-water solution. Besides, the susceptibility of bagasse for cellulolytic enzymatic hydrolysis is improved. The highest hemicellulose precipitation yield was 85% which corresponded to the treatment with the highest alkali charge without peroxide. The highest yield of enzymatic hydrolysis was obtained for the highest alkali and peroxide charges.

Coproduction of xylooligosaccharides and fermentable sugars from sugarcane bagasse by seawater hydrothermal pretreatment

In this study, natural seawater without additional chemicals was selected to treat sugarcane bagasse for the production of xylooligosaccharides and glucose. This pretreatment not only more effectively conserves freshwater resources than hydrothermal pretreatment and enzymatic hydrolysis, but also decreases corrosion of the equipment relative to techniques utilizing acid and alkaline pretreatment. The maximum yield of 67.12% xylooligosaccharides (of initial xylan), including 11.49% xylobiose, 16.23% xylotriose, 23.82% xylotetraose, and 15.58% xylopentaose was obtained under mild condition (175 °C for 30 min). Moreover, greater amounts of xylotetraose were generated during seawater hydrothermal pretreatment under all conditions, likely because NaCl in seawater cut the hydrogen bonds between xylo-oligomers. In addition, 94.69% cellulose digestibility and 78.58% xylan digestibility were achieved from the solid residue with an enzyme dosage of 30 FPU/g cellulose. Results indicated that seawater hydrothermal pretreatment is a more environmentally-friendly and sustainable technique for producing xylooligosaccharides and fermentable sugars than other methods.

Treatment of laundry wastewater using chemically treated sugarcane bagasse

Laundry wastewater constitutes of detergent compounds, bleaching agent, textile color pigments and dirt. These compounds are hazardous once discharged into the water source without proper treatment due to the adverse effects on the aquatic life in the freshwater bodies. This fixed-bed column adsorption study using the natural biomass from chemically treated sugarcane bagasse is proven to be effective for the removal of constituents present in the laundry wastewater. The synthetic laundry wastewater was prepared in the laboratory from a powder type of detergent with a concentration of 50mg/L and 250mg/L to be further used in the adsorption process. The characteristics of the synthetic laundry wastewater were analyzed by the turbidity, pH, dissolved oxygen (DO) and chemical oxygen demand (COD). The fixed-bed column experiment with the treated sugarcane bagasse was conducted under different parameters such as the effect of pH, initial concentration and bed height. The best removal efficiency was observed at a bed height of 10 cm with a concentration of 50 mg/L is 65%, followed by 33% and 20% for bed heights of 4 and 2 cm respectively. As for the concentration of 250 mg/L, the best removal efficiency was at a bed height of 10 cm which was found to be 58% followed by 26% and 17% removal for a bed height of 4 cm and 2 cm respectively. The kinetics of the adsorption was investigated using the kinetic models of Thomas and Yoon-Nelson. The experimental results fitted well on the Thomas model and Yoon-Nelson which showed a high linear regression value greater than 0.9. These results prove that chemically treated sugarcane bagasse is an effective low-cost adsorbent for reducing turbidity, pH, COD and increasing the DO concentration.

Exploring why sodium lignosulfonate influenced enzymatic hydrolysis efficiency of cellulose from the perspective of substrate\u2013enzyme adsorption

BackgroundCellulase adsorbed on cellulose is productive and helpful to produce reducing sugars in enzymatic hydrolysis of lignocellulose; however, cellulase adsorbed on lignin is non-productive. Increasing productive adsorption of cellulase on cellulose would be beneficial in improving enzymatic hydrolysis. Adding lignin that was more hydrophilic in hydrolysis system could increase productive adsorption and promote hydrolysis. However, the effect mechanism is still worth exploring further. In this study, lignosulfonate (LS), a type of hydrophilic lignin, was used to study its effect on cellulosic hydrolysis.ResultsThe effect of LS on the enzymatic hydrolysis of pure cellulose (Avicel) and lignocellulose [dilute acid (DA) treated sugarcane bagasse (SCB)] was investigated by analyzing enzymatic hydrolysis efficiency, productive and non-productive cellulase adsorptions, zeta potential and particle size distribution of substrates. The result showed that after adding LS, the productive cellulase adsorption on Avicel reduced. Adding LS to Avicel suspension could form the Avicel–LS complexes. The particles were charged more negatively and the average particle size was smaller than Avicel before adding LS. In addition, adding LS to cellulase solution formed the LS–cellulase complexes. For DA-SCB, adding LS decreased the non-productive cellulase adsorption on DA-SCB from 3.92 to 2.99 mg/g lignin and increased the productive adsorption of cellulase on DA-SCB from 2.00 to 3.44 mg/g cellulose. Besides, the addition of LS promoted the formation of LS–lignin complexes and LS–cellulase complexes, and the complexes had more negative charges and smaller average sizes than DA-SCB lignin and cellulase particles before adding LS.ConclusionsIn this study, LS inhibited Avicel’s hydrolysis, but enhanced DA-SCB’s hydrolysis. This stemmed from the fact that LS could bind cellulase and Avicel, and occupied the binding sites of cellulase and Avicel. Thus, a decreased productive adsorption of cellulase on Avicel arose. Regarding DA-SCB, adding LS, which enhanced hydrolysis efficiency of DA-SCB, increased the electrostatic repulsion between DA-SCB lignin and cellulase, and therefore, decreased non-productive adsorption of cellulase on DA-SCB lignin and enhanced productive adsorption of cellulase on DA-SCB cellulose.Graphical abstract

Exploring surface properties of substrate to understand the difference in enzymatic hydrolysis of sugarcane bagasse treated with dilute acid and sulfite

The sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) is beneficial to subsequent enzymatic hydrolysis. In this study, to elucidate enzymatic hydrolysis mechanism of SPORL treated sugarcane bagasse (SCB), the surface properties of SPORL and dilute acid (DA) treated SCBs were compared, including cellulase adsorption isotherm and kinetics, specific surface area and average size of pores, water retention value (WRV), element composition and percentages of different chemical bonds on the substrate surface. The results showed the hydrolysis yield of SPORL SCB (76.16 %) was higher than that of DA (57.82 %) and SPORL SCB had better adsorption capacity, lower binding strength, a higher cellulase adsorption rate, larger specific surface area and higher WRV, and these two SCBs might adsorb cellulase by COR bonds (R: organic groups), suggesting SPORL SCB’s surface was more advantageous to enzymatic hydrolysis. This study could help understand the mechanism of lignocellulosic hydrolysis in depth.

A stepwise pretreatment of sugarcane bagasse by alkaline and hydroxymethyl reagent for bioethanol production

Reducing the lignin inhibition in the enzymatic digestibility is essential to obtain high yields of sugar and bioethanol. The sequential NaOH and hydroxymethylation pretreatment (AHP) were used to treat sugarcane bagasse (SCB). Compared with NaOH pretreated SCB alone (AP-SCB), AHP increased the yield of glucose and xylose from 53.3%–68.88 %, from 67.8%–74.7 %, respectively. The hydrolysate was fermented using Saccharomyces cerevisiae for 24 h, and then ethanol concentration was 10.67 g/L, which increased by approximately 13 % compared with that of AP-SCB. The post-treatment of hydroxymethylation introduced hydroxymethyl groups and hydroxylic groups into in the aromatic nucleus of lignin, increasing the hydrophilicity and solubility of lignin in materials, which can reduce the nonproductive adsorption of lignin on cellulases and thereby improve the enzymatic saccharification efficiency.

Synthesis and characterization of microwave absorbing material

The present work encloses the application of ultrasonically blended chemicals in the treatment of waste materials like sugarcane bagasse fiber for presynthesis of microwaves absorbing material/radar absorbing material for different applications. The optimized blended chemicals with alcohol blended acrylic acid are determined by computation of different acoustic parameters with the propagation of ultrasonic wave in frequency ranges from 1 to 5 MHz. The optimum blend of acrylic acid with methanol with 0.4 concentrations, out of all other alcohol group, shows its best characteristics in all frequency ranges for the modification of surface of sugarcane bagasse. The study of elemental dispersion images of untreated and treated sugarcane bagasse fiber signifies the suitability of the material for synthesis of microwave absorbing materials. The presence of many reaction sites on the treated sugarcane bagasse fiber makes its suitability for synthesis of biomaterial. The SEM of treated sugarcane bagasse fiber gives significant explanation for absorption of incident microwave radiation on the biomaterial prepared from this waste. The reflection loss of the synthesized biocomposite shows significant effect of the treatment of ultrasonic wave for microwave absorption at 10 GHz in X-band frequency range.