PURPOSE: To compare maximum oxygen consumption acquired by two maximum cardiopulmonary exercise tests with fifteen minutes of recovery between them. METHODS: Eleven physically healthy volunteers performed two maximal treadmill ramp protocols (the protocol was programmed to achieve a volitional fatigue by the volunteer within 10 ± 2 min.) with a 15 minutes of recovery between them. The treadmill grade and velocity varied according with the maximum predicted oxygen uptake (VO2 max). The interval between tests were executed with 2 minutes walking (4 km/h), followed by 2 minutes in orthostatic position and 11 minutes seated, accomplishing 15 minutes of recovery. Time (TT1, TT2) and VO2max (VO2max1, VO2max2) performed in both tests were analyzed. Statistical analysis was used Kolmogorov-Smirnov method obtaining a normal distribution, Students t-test was used for repeated measures which did not show significant difference in the variable time, but have shown in VO2 max. For statistical probability was considered P < 0,05. RESULTS: TT1 (min): 12,33 ± 1,07; TT2: 12,66 ± 0,91; VO2 max1 (ml.kg.min−1): 52 ± 3,82; VO2 max2: 54 ± 3,52. CONCLUSIONS: The sequenced application of two maximum cardiopulmonary exercise tests in treadmill using a ramp protocol with a 15 minutes of recovery between them resulted an higher value of VO2max at the second test however the mean time of tests did not show a significant difference. Further studies are necessaries in order to elucidate it, with larger number of subjects and more controlled variables.