Abstract: Even if βAPP remains the gold standard in identifying diffuse axonal injuries, other histological, ultrastructure, histochemical, or immunohistochemical markers may aid the diagnosis and may better characterize this traumatic brain pathology. Moreover, associated changes, including neuronal body changes, the presence of microglial clusters, and so on, when identified in a traumatic context, should suggest at least the need of using specific markers for identification of DAIs. The purpose of this article is to present the main microscopy techniques used in forensic practice to detect diffuse axonal injury. Key Words: diffuse axonal injury, beta-APP, ubiquitin, forensic pathology. 1) (a) MD, National Institute of Legal Medicine, Bucharest, Romania; (b) Lecturer, “Carol Davila” University of Medicine and Pharmacy, Dept of Legal Medicine and Bioethics, Bucharest, Romania *Corresponding author: Sos. Vitan Barzesti 9, 042122, Sector 4 Bucharest, Romania, Tel. +40-723-791072, Email: soraer@gmail.com, sorin.hostiuc@legmed.ro2) Lecturer, MD, Research Center for Microscopic Morphology and Immunology, Department of Morphology, University of Medicine and Pharmacy of Craiova, Craiova, Romania3) (a) MD, Floreasca Clinical Emergency Hospital, Bucharest, Romania, (b) Assist.Prof., “Carol Davila” University of Medicine and Pharmacy, Dept of Surgery, Bucharest, Romania4) Prof, “Carol Davila” University of Medicine and Pharmacy, Dept of Physiology, Bucharest, Romania5) (a) MD, National Institute of Legal Medicine, Bucharest, Romania; (b) Lecturer, “Carol Davila” University of Medicine and Pharmacy, Dept of Pathology, Bucharest, Romania