ABSTRACTTrauma-informed schools are working to mitigate the effects of childhood trauma across the life span (NCTSN, 2008). While universal screenings are recommended as part of a trauma-informed framework (Ko et al, 2008), there are few developed models on how to implement universal trauma screenings in schools. In an effort to prevent and treat childhood trauma, Wediko Children’s Services (Wediko) developed a school-based universal childhood trauma screening protocol. The use of the Adverse Childhood Experiences (ACE) Questionnaire as the screening tool to identify each student’s ACE score, as well as how the gained knowledge from these screenings was then utilized to treat trauma, is discussed. With a focus on supporting urban, low-income schools, empirical research examining the ACE Questionnaire, trauma-informed care and universal screenings is reviewed through an implementation framework. This theoretical paper offers Wediko as a case study to assist other schools and community-based mental health agencies in administering universal screenings in their schools with a focus on how to support the initial implementation phase.