The results of a survey of Doryctinae (Hymenoptera: Braconidae), excluding species of Heterospilus Haliday, at Konza Prairie near Manhattan, Kansas are reported. Eleven sites representing prairie and woodland/wetland areas, including gallery forest, were sampled in 2001 and 2005 using Malaise and canopy traps. Topographic trap placement included lowland, midslope, and upland areas. Twentyfour species were collected, including 18 in 2001 and 16 in 2005. Twenty-three of the 24 species were collected from the woodland/wetland sites, including 19 from gallery forest sites; five of the 24 species were collected from the prairie sites. Rhaconotus fasciatus (Ashmead) was the most abundant species in 2001 (n = 13); Callihormius bifasciatus (Ashmead) and Coiba jeffersoni Kula were the most abundant species in 2005 (n = 10). The following new species are described: Doryctes xanthogaster Kula and Marsh, Doryctes xanthosoma Kula and Marsh, Doryctinus zolnerowichi Kula and Marsh, and Pambolidea dollari Kula and Marsh. Glyptocolastes caryae (Ashmead), status revised is removed from synonymy with Glyptocolastes rugulosus (Cresson), the type species for Doryctinus Roman. The latter species is transferred to Acrophasmus Enderlein, resulting in Acrophasmus as a new synonym of Doryctinus. Synonymy of the aforementioned genera results in the following nomenclatural changes: Doryctinus amazonicus (Roman), new combination; Doryctinus arizonensis (Marsh), new combination; Doryctinus atriventris (Cresson), new combination; Doryctinus butleri (Marsh), new combination; Doryctinus costaricensis (Marsh), new combination; Doryctinus erugatus (Marsh), new combination; Doryctinus exilis (Enderlein), new combination; Doryctinus ferrugineus (Marsh), new combination; Doryctinus gauldi (Marsh), new combination; Doryctinus immigrans (Beardsley), new combination; Doryctinus maeandrius (Enderlein), new combination; Doryctinus marshi Greenbaum, revised combination; Doryctinus rubronotum (Marsh), new combination; Doryctinus rugulosus (Cresson), revised combination; Doryctinus scobiciae (Marsh), new combination; and Doryctinus secundus (Muesebeck and Walkley), new combination. Doryctes infuscus Marsh is a new synonym of Doryctes rufipes (Provancher), Pioscelus wichitus (Viereck) is a new synonym of Pioscelus borealis (Ashmead), and Rhaconotus graciliformis (Viereck) is a new synonym of R. fasciatus. The following species are first records for Kansas: C. bifasciatus, Dendrosoter sulcatus Muesebeck, D. rufipes, D. ferrugineus, Ecphylus hypothenemi Ashmead, Ecphylus rohweri Muesebeck, Ontsira mellipes (Ashmead), and Rhaconotus canadensis Marsh.