Transport Of NO3 Research Articles

The Arabidopsis thaliana ecotype Ct-1 achieves higher salt tolerance relative to Col-0 via higher tissue retention of K+ and NO3-

Agriculture is vital for global food security, and irrigation is essential for improving crop yields. However, irrigation can pose challenges such as mineral scarcity and salt accumulation in the soil, which negatively impact plant growth and crop productivity. While numerous studies have focused on enhancing plant tolerance to high salinity, research targeting various ecotypes of Arabidopsis thaliana has been relatively limited. In this study, we aimed to identify salt-tolerant ecotypes among the diverse wild types of Arabidopsis thaliana and elucidate their characteristics at the molecular level. As a result, we found that Catania-1 (Ct-1), one of the ecotypes of Arabidopsis, exhibits greater salt tolerance compared to Col-0. Specifically, Ct-1 exhibited less damage from reactive oxygen species (ROS) than Col-0, despite not accumulating antioxidants like anthocyanins. Additionally, Ct-1 accumulated more potassium ions (K+) in its shoots and roots than Col-0 under high salinity, which is crucial for water balance and preventing dehydration. In contrast, Ct-1 plants were observed to accumulate slightly lower levels of Na+ than Col-0 in both root and shoot tissues, regardless of salt treatment. These findings suggest that Ct-1 plants achieve high salinity resistance not by extruding more Na+ than Col-0, but rather by absorbing more K+ or releasing less K+. Ct-1 exhibited higher nitrate (NO3-) levels than Col-0 under high salinity conditions, which is associated with enhanced retention of K+ ions. Additionally, genes involved in NO3- transport and uptake, such as NRT1.5 and NPF2.3, showed higher transcript levels in Ct-1 compared to Col-0 when exposed to high salinity. However, Ct-1 did not demonstrate significantly greater resistance to osmotic stress compared to Col-0. These findings suggest that enhancing plant tolerance to salt stress could involve targeting the cellular processes responsible for regulating the transport of NO3- and K+. Overall, our study sheds light on the mechanisms of plant salinity tolerance, emphasizing the importance of K+ and NO3- transport in crop improvement and food security in regions facing salinity stress.

Multiple isotopes reveal the impact of land use change on nitrate transport and transformation in deep loess deposits

The deep-rooted economic trees decrease water storage but increase nitrate-nitrogen (NO3–N) accumulation in the soil due to the root water uptake and excessive use of fertilizers. Knowing how the NO3 accumulates in the soil and to what extent it can be flushed out or transformed can provide insights on the processes controlling N flux to waterways. We sampled cultivated farmland and orchards with varying ages of apple and peach trees in the loess deposits to > 15 m depth to measure the contents and isotopes of water and NO3 in soils. The novelty of this study is that the water isotopes are used to analyze water and NO3 transport, while the NO3 isotopes are further employed for NO3 source and transformation analysis. The parabolic NO3–N profiles (258–3579 kg N ha−1) were mainly observed within 0–5 m under different land use types. The surface-aggregated NO3 reservoirs under old apple orchard were about six times of farmland due to the overuse of chemical fertilizers, while those of peach orchard were only half of farmland because of less fertilizer inputs and possible root absorption effects. Piston flow resulted in the overall slow water movement and low NO3 flux under different land use types in deep loess deposits. Despite the soil water recharge by precipitation and low surface evaporation, the strong transpiration effects further resulted in no water and NO3 fluxes in the deep layers under old apple orchard. Nitrate reservoirs were mainly contributed by soil organic N (44 % ± 26 %) and ammonium N fertilizer (36 % ± 20 %), but their transformation was dominated by nitrification under old apple orchard and by mineralization in deep layers. The risks of NO3 bombs are temporarily weakened by lower dried soil layers under deep rooted plants; however, with lower water movement rates and limited denitrification potential, NO3 would eventually reach the aquifers in a few hundred years or more. This study provides new insights for clarifying NO3 transport and transformation processes by combing multiple isotopes, and the NO3 bombs would chronically endanger the sustainable development of terrestrial ecosystems and the environment in arid regions.

Climate and landform interact to control the source and transport of nitrate in Pacific Northwest rivers

The hydrological effects of climate change are documented in many regions; however, climate-driven impacts to the source and transport of river nutrients remain poorly understood. Understanding the factors controlling nutrient dynamics across river systems is critical to preserve ecosystem function yet challenging given the complexity of landscape and climate interactions. Here, we harness a large regional dataset of nitrate (NO3–) yield, concentration, and isotopic composition (δ15N and δ18O) to evaluate the strength of hydroclimate and landscape variables in controlling the seasonal source and transport of NO3–. We show that hydroclimate strongly influenced the seasonality of river NO3–, producing distinct, source-dependent NO3– regimes across rivers from two mountain ranges. Riverine responses to hydroclimate were also constrained by watershed-scale topographic features, demonstrating that while regional climate strongly influences the timing of river NO3– transport, watershed topography plays a distinct role in mediating the sensitivity of river NO3– dynamics to future change.

Unraveling differential characteristics and mechanisms of nitrogen uptake in wheat cultivars with varied nitrogen use efficiency

Nitrogen uptake is crucial to wheat nitrogen use efficiency (NUE). The study's findings indicate that both high- and low-NUE cultivars exhibited highest nitrogen uptake efficiency (NupE) under 0.2 mM nitrogen. Under 2 mM nitrogen, their NupEs decrease significantly, while uptakes to NO3− were notably higher than that of NH4+. Strikingly, high-NUE cultivars exhibited a significantly higher NH4+ uptake rate than low NUE cultivars, resulting in a marked improvement in their ability to take up nitrogen. The NUEs of the cultivars with 5 mM nitrogen were almost half that of 2 mM nitrogen. NO3− uptake primarily occurred in the mature zone of roots, while NH4+ uptake took place in the root tip meristem and elongation zones. Notably, the NH4+ uptake in root tip meristematic zone of high-NUE cultivar was significantly higher than that of low NUE cultivar. Furthermore, the NO3− uptake of high-NUE cultivar in the root mature zone was significantly higher than that of low-NUE cultivar under 2 mM nitrogen. These findings were consistent with the significantly higher expression levels of TaAMT in root tip and of TaNRT in root mature area of high-NUE cultivar compared to low-NUE cultivar, respectively, enabling efficient absorption of NO3− and NH4+ and transport of NO3− to shoot. The high-NUE cultivars showed elevated expression of amino acid transporters further promoting nitrogen uptake, and conversion of nitrogen into ureides and amino acids further facilitated inorganic nitrogen uptake by roots. The differential findings offer valuable insights into novel variety breeding of high NUE in the future.

The K+ transporter NPF7.3/NRT1.5 and the proton pump AHA2 contribute to K+ transport in Arabidopsis thaliana under K+ and NO3- deficiency.

Nitrate (NO3 -) and potassium (K+) are distributed in plants via short and long-distance transport. These two pathways jointly regulate NO3 - and K+ levels in all higher plants. The Arabidopsis thaliana transporter NPF7.3/NRT1.5 is responsible for loading NO3 - and K+ from root pericycle cells into the xylem vessels, facilitating the long-distance transport of NO3 - and K+ to shoots. In this study, we demonstrate a protein-protein interaction of NPF7.3/NRT1.5 with the proton pump AHA2 in the plasma membrane by split ubiquitin and bimolecular complementation assays, and we show that a conserved glycine residue in a transmembrane domain of NPF7.3/NRT1.5 is crucial for the interaction. We demonstrate that AHA2 together with NRT1.5 affects the K+ level in shoots, modulates the root architecture, and alters extracellular pH and the plasma membrane potential. We hypothesize that NRT1.5 and AHA2 interaction plays a role in maintaining the pH gradient and membrane potential across the root pericycle cell plasma membrane during K+ and/or NO3 - transport.

The preferential transport of NO3− by full-length Guillardia theta anion channelrhodopsin 1 is enhanced by its extended cytoplasmic domain

Previous research of anion channelrhodopsins (ACRs) has been performed using cytoplasmic domain (CPD)-deleted constructs and therefore have overlooked the native functions of full-length ACRs and the potential functional role(s) of the CPD. In this study, we used the recombinant expression of full-length Guillardia theta ACR1 (GtACR1_full) for pH measurements in Pichia pastoris cell suspensions as an indirect method to assess its anion transport activity and for absorption spectroscopy and flash photolysis characterization of the purified protein. The results show that the CPD, which was predicted to be intrinsically disordered and possibly phosphorylated, enhanced NO3- transport compared to Cl- transport, which resulted in the preferential transport of NO3-. This correlated with the extended lifetime and large accumulation of the photocycle intermediate that is involved in the gate-open state. Considering that the depletion of a nitrogen source enhances the expression of GtACR1 in native algal cells, we suggest that NO3- transport could be the natural function of GtACR1_full in algal cells.

Spatial and Temporal Analysis of Nitrate Dynamics along the Tigris River

Given the wide dependency on surface water used to supply drinking water, agricultural irrigation, and industrial activities, nitrate pollution has posed a serious concern in the Tigris River in recent years. The main objective of this study was to develop an understanding of the spatiotemporal patterns of nitrate distribution in the Tigris River through an integrated approach using hydrological data, physicochemical parameters, and model-based analysis. Eighty-four monthly sampling campaigns from forty monitoring locations along the Tigris River were carried out from January 2011 to December 2018. Obtained results demonstrated that the NO3- dynamics were strongly correlated with the length of transport distance and flow rates along the river system (p < 0.05). High flow rates in the upper courses of the river system favored physical transport of NO3- and promoted a dilution effect. However, low flow rates in the lower sections favored the accumulation processes of NO3- and promoted a concentration effect. High concentration of 7.0±1.96 g NO3- m-3 was observed in February 2018 downstream in the river. No significant seasonal effect in NO3- concentrations were observed. These results were supported by the changes in dissolved oxygen concentration and pH in the river system and indicated high nitrification rates and elevated NO3- accumulation, particularly downstream in the river. This modeling approach has also confirmed field observations of NO3- dynamics with 65% of the variances in the river system being explained by the model.

Significant Latitudinal Gradient of Nitrate Production in the Marine Atmospheric Boundary Layer of the Northern Hemisphere

AbstractAtmospheric samples collected during 2017 and 2018 Chinese Arctic Expedition cruises are used to investigate the latitudinal gradients of nitrate (NO3−) sources and production in the marine atmospheric boundary layer of the Northern Hemisphere. Both concentration and isotopic composition (δ15N, δ18O, and Δ17O) of atmospheric NO3− show significant latitudinal gradients. δ15N suggests that continental sources dominate atmospheric NO3− in both mid‐and high‐latitudes, while oceanic RONO2 emissions account for 21%–26% of the NO3− budget in mid‐latitudes. Slightly higher δ15N in high‐latitudes than in mid‐latitudes indicates insignificant isotope effects of long‐range atmospheric transport of NO3−. δ18O and Δ17O of NO3− are significantly higher in mid‐latitudes than in high‐latitudes, suggesting distinct main production pathways in the two regions. It is estimated that ∼69% of NO3− is produced via NO2 + OH in the high‐latitudes, while ∼68% of NO3− is from N2O5 + H2O and NO3 + DMS/CH pathways in mid‐latitudes.

AtbZIP62 Acts as a Transcription Repressor to Positively Regulate ABA Responses in Arabidopsis.

The basic region/leucine zipper (bZIP) transcription factor AtbZIP62 is involved in the regulation of plant responses to abiotic stresses, including drought and salinity stresses, NO3 transport, and basal defense in Arabidopsis. It is unclear if it plays a role in regulating plant responses to abscisic acid (ABA), a phytohormone that can regulate plant abiotic stress responses via regulating downstream ABA-responsive genes. Using RT-PCR analysis, we found that the expression level of AtbZIP62 was increased in response to exogenously applied ABA. Protoplast transfection assays show that AtbZIP62 is predominantly localized in the nucleus and functions as a transcription repressor. To examine the roles of AtbZIP62 in regulating ABA responses, we generated transgenic Arabidopsis plants overexpressing AtbZIP62 and created gene-edited atbzip62 mutants using CRISPR/Cas9. We found that in both ABA-regulated seed germination and cotyledon greening assays, the 35S:AtbZIP62 transgenic plants were hypersensitive, whereas atbzip62 mutants were hyposensitive to ABA. To examine the functional mechanisms of AtbZIP62 in regulating ABA responses, we generated Arabidopsis transgenic plants overexpressing 35S:AtbZIP62-GR, and performed transcriptome analysis to identify differentially expressed genes (DEGs) in the presence and absence of DEX, and found that DEGs are highly enriched in processes including response to abiotic stresses and response to ABA. Quantitative RT-PCR results further show that AtbZIP62 may regulate the expression of several ABA-responsive genes, including USP, ABF2, and SnRK2.7. In summary, our results show that AtbZIP62 is an ABA-responsive gene, and AtbZIP62 acts as a transcription repressor to positively regulate ABA responses in Arabidopsis.

Fine particulate pollution driven by nitrate in the moisture urban atmospheric environment in the Pearl River Delta region of south China

To identify potential sources of fine particles (PM2.5, with aerodynamic diameter (Da) ≤ 2.5 μm) in urban Dongguan of south China, a comprehensive campaign was carried out in the whole 2019. Hourly PM2.5 and its dominant chemical components including organic carbon (OC), elemental carbon (EC), water-soluble inorganic ions (WSIIs) and thirteen elements were measured using online instruments. Gaseous pollutants including NH3, HNO3, NO2, NO and O3 and meteorological parameters were also synchronously measured. PM2.5 was dominated by carbonaceous aerosols in summer and by WSIIs in the other seasons. PM2.5 and its dominant chemical components mostly peaked around noon (10:00–14:00 LST). Furthermore, high PM2.5 levels during the daytime were closely related with the increased NO3− levels. The high mass concentrations of NO3− in urban Dongguan during the daytime were likely related with regional transport of NO3− from suburban Dongguan, which was originated from the reaction between NO2 and O3 under the moisture condition during the nighttime. Seven major source factors for PM2.5 including secondary sulfate, ship emission, traffic emission, secondary nitrate, industrial processes, soil dust and coal combustion were identified by positive matrix factorization (PMF) analysis, which contributed 26 ± 14%, 16 ± 16%, 16 ± 10%, 14 ± 11%, 12 ± 11%, 8 ± 6% and 8 ± 6%, respectively, to annual PM2.5 mass concentration. Although secondary sulfate contributed much more than secondary nitrate to PM2.5 on annual basis, the latter exceeded the former source factor when daily PM2.5 mass concentration was higher than 60 μg m−3, indicating the critical role nitrate played in PM2.5 episode events.

Guard cell anion channel PbrSLAC1 regulates stomatal closure through PbrSnRK2.3 protein kinases

Stomatal pores on the leaf surface are the gateways for gas exchange between plants and the atmosphere, which is regulated mainly by the S-type anion channel SLAC1. However, the gene encoding the main S-type anion channel SLAC1 in pear and its genetic characteristics remain unknown. In this study, Pbr015894.1 was identified as the candidate for PbrSLAC1 in pear, and it was found to be expressed abundantly in leaves, particularly in the guard cells. Virus-induced gene silencing experiments indicated that stomatal closure was achieved by a change in cell turgor instigated by PbrSLAC1 channel transport of NO3− in pear leaves and induced by abscisic acid. Furthermore, the expression of PbrSLAC1 in Arabidopsis slac1–3 and slac1–4 rescued the defective NO3− transport seen in these mutants, pointing to its role in anion transport. Fluorescence microscopy suggested that PbrSLAC1 was localized in the plasma membrane, and a dual-luciferase assay system demonstrated an interaction between PbrSLAC1 and PbrSnRK2.3/2.8. Moreover, anion conductance mediated by PbrSLAC1 was activated by PbrSnRK2.3 in Xenopus laevis oocytes and the channel showed greater permeability for nitrate than chloride, sulfate, or malate ions. Taken together, these results demonstrate that PbrSLAC1, an anion channel regulated by PbrSnRK2.3, is involved in stomatal closure by mediating the efflux of NO3− in pear leaf.

Seasonal modeling analysis of nitrate formation pathways in Yangtze River Delta region, China

Abstract. Nitrate (NO3−) has been the dominant and the least reduced chemical component of fine particulate matter (PM2.5) since the stringent emission controls implemented in China in 2013. The formation pathways of NO3− vary seasonally and differ substantially in daytime vs. nighttime. They are affected by precursor emissions, atmospheric oxidation capacity, and meteorological conditions. Understanding NO3− formation pathways provides insights for the design of effective emission control strategies to mitigate NO3− pollution. In this study, the Community Multiscale Air Quality (CMAQ) model was applied to investigate the impact of regional transport, predominant physical processes, and different formation pathways to NO3− and total nitrate (TNO3, i.e., HNO3+ NO3−) production in the Yangtze River Delta (YRD) region during the four seasons of 2017. NO3-/PM2.5 and NO3-/TNO3 are the highest in the winter, reaching 21 % and 94 %, respectively. The adjusted gas ratio (adjGR = ([NH3]+ [NO3−])/([HNO3]+ [NO3−])) in the YRD is generally greater than 2 in the four seasons across most areas in the YRD, indicating that YRD is mostly in the NH3-rich regime and that NO3− is limited by HNO3 formation. Local emissions and regional transportation contribute to NO3− concentrations throughout the YRD region by 50 %–62 % and 38 %–50 %, respectively. The majority of the regional transport of NO3− concentrations is contributed by indirect transport (i.e., NO3− formed by transported precursors reacting with local precursors). Aerosol (AERO, including condensation, coagulation, new particle formation, and aerosol growth) processes are the dominant source of NO3− formation. In summer, NO3− formation is dominated by AERO and total transport (TRAN, sum of horizontal and vertical transport) processes. The OH + NO2 pathway contributes to 60 %–83 % of the TNO3 production, and the N2O5 heterogeneous (HET N2O5) pathway contributes to 10 %–36 % in the YRD region. HET N2O5 contribution becomes more important in cold seasons than warm seasons. Within the planetary boundary layer in Shanghai, the TNO3 production is dominated by the OH + NO2 pathway during the day (98 %) in the summer and spring and by the HET N2O5 pathway during the night (61 %) in the winter. Local contributions dominate the OH + NO2 pathway for TNO3 production during the day, while indirect transport dominates the HET N2O5 pathway at night.

Strategies to increase ion selectivity in electrodialysis

We analyze selective removal of anions in electrodialysis (ED) from a multicomponent ion mixture where all ions are monovalent, both experimentally, and by a modified theory. The theory makes use of the Nernst-Planck equation to describe transport of ions across ion-exchange membranes in combination with the ion affinity, which is a parameter that accounts for the preference of membrane materials to adsorb one ion more than another. For a mixture of NO3- and Cl- anions, ion affinity was measured in an adsorption experiment, and it was found that NO3- adsorbs more in anion-exchange membranes (AEMs) than Cl-. Faster transport of NO3- relative to Cl- in batch-mode ED experiments is well described by the transport theory, for three types of AEMs that we tested. From analysis of the model, we can derive that membrane selectivity in ED between different anions can be increased further by: i) an increase in the difference in ion affinity of the anions, ii) an increase in the AEM thickness, and iii) a decrease in the charge density of the AEM. The last two strategies go against the general understanding that thin highly charged membranes are in general better for ED. Our proposed strategies follow rigorously from our combined theoretical–experimental study.

Surface water temperature impacts on coastal wetland denitrification: Implications for river reconnection

Louisiana, located in the southeast United States, is home to 40% of the continental US's coastal wetlands yet accounts for 80% of the nation's coastal wetland loss. This loss is generally attributed to decreased sediment supply, hydrologic alteration from levees, channelization, subsidence, sea-level rise, and wave and tidal induced marsh edge erosion. The Mid-Barataria Sediment Diversion is a US $1.3 billion coastal restoration project that will divert up to 2100 m3 s−1 of sediment-laden Mississippi River water directly into Barataria Basin. The influx of colder, nutrient-rich, springtime river water could negatively impact water quality of the receiving basin. We quantified the effects of colder, surface water temperature on the nitrate (NO3−) reduction rate in vegetated marsh and open water bay sediments. Colder water limited NO3− removal processes averaging 17.1 mg N m−2 d−1 in the range of 5–14 °C, before increasing almost 3-fold in the 20 °C treatments at 50.6 mg N m−2 d−1. Low N removal rates, especially near the project inflow where temperatures will be coldest will favor transport of NO3− further into Barataria Basin where eutrophic conditions could become expressed. These results will inform coastal managers around the world of the potential ecosystem response to coastal restoration aimed at river reconnection where colder waters enter warmer, shallow basins.

Using Transcript Levels of Nitrate Transporter 2 as Molecular Indicators to Estimate the Potentials of Nitrate Transport in Symbiodinium, Cladocopium, and Durusdinium of the Fluted Giant Clam, Tridacna squamosa

Giant clams are important ecosystem engineers of coral reefs because they harbor large quantities of phototrophic Symbiodiniaceae dinoflagellates of mainly genera Symbiodinium, Cladocopium, and Durusdinium. The coccoid dinoflagellates donate photosynthate and amino acids to the clam host, which in return needs to supply inorganic carbon and nitrogen to them. The host can conduct light-enhanced absorption of nitrate (NO3–), which can only be metabolized by the symbionts. This study aimed to clone nitrate transporter 2 (NRT2) from the symbionts of the fluted giant clam, Tridacna squamosa. Here, we report three major sequences of NRT2 derived from Symbiodinium (Symb-NRT2), Cladocopium (Clad-NRT2) and Durusdinium (Duru-NRT2). Phenogramic analysis and molecular characterization confirmed that these three sequences were NRT2s derived from dinoflagellates. Immunofluorescence microscopy localized NRT2 at the plasma membrane and cytoplasmic vesicles of the symbiotic dinoflagellates, indicating that it could partake in the uptake and transport of NO3–. Therefore, the transcript levels of Symb-NRT2, Clad-NRT2, and Duru-NRT2 could be used as molecular indicators to estimate the potential of NO3– transport in five organs of 13 T. squamosa individuals. The transcript levels of form II ribulose-1, 5-bisphosphate carboxylase/oxygenase (rbcII) of Symbiodinium (Symb-rbcII), Cladocopium (Clad-rbcII) and Durusdinium (Duru-rbcII) were also determined in order to calculate the transcript ratios of Symb-NRT2/Symb-rbcII, Clad-NRT2/Clad-rbcII, and Duru-NRT2/Duru-rbcII. These ratios expressed the potentials of NO3– transport with reference to the phototrophic potentials in a certain genus of coccoid dinoflagellate independent of its quantity. Results obtained indicate that Symbiodinium generally had a higher potential of NO3– transport than Cladocopium and Durusdinium at the genus level. Furthermore, some phylotypes (species) of Symbiodinium, particularly those in the colorful outer mantle, had very high Symb-NRT2/Symb-rbcII ratio (7–13), indicating that they specialized in NO3– uptake and nitrogen metabolism. Overall, our results indicate for the first time that different phylotypes of Symbiodiniaceae dinoflagellates could have dissimilar abilities to absorb and assimilate NO3–, alluding to their functional diversity at the genus and species levels.

Regulation of Nitrate (NO3) Transporters and Glutamate Synthase-Encoding Genes under Drought Stress in Arabidopsis: The Regulatory Role of AtbZIP62 Transcription Factor.

Nitrogen (N) is an essential macronutrient, which contributes substantially to the growth and development of plants. In the soil, nitrate (NO3) is the predominant form of N available to the plant and its acquisition by the plant involves several NO3 transporters; however, the mechanism underlying their involvement in the adaptive response under abiotic stress is poorly understood. Initially, we performed an in silico analysis to identify potential binding sites for the basic leucine zipper 62 transcription factor (AtbZIP62 TF) in the promoter of the target genes, and constructed their protein–protein interaction networks. Rather than AtbZIP62, results revealed the presence of cis-regulatory elements specific to two other bZIP TFs, AtbZIP18 and 69. A recent report showed that AtbZIP62 TF negatively regulated AtbZIP18 and AtbZIP69. Therefore, we investigated the transcriptional regulation of AtNPF6.2/NRT1.4 (low-affinity NO3 transporter), AtNPF6.3/NRT1.1 (dual-affinity NO3 transporter), AtNRT2.1 and AtNRT2.2 (high-affinity NO3 transporters), and AtGLU1 and AtGLU2 (both encoding glutamate synthase) in response to drought stress in Col-0. From the perspective of exploring the transcriptional interplay of the target genes with AtbZIP62 TF, we measured their expression by qPCR in the atbzip62 (lacking the AtbZIP62 gene) under the same conditions. Our recent study revealed that AtbZIP62 TF positively regulates the expression of AtPYD1 (Pyrimidine 1, a key gene of the de novo pyrimidine biosynthesis pathway know to share a common substrate with the N metabolic pathway). For this reason, we included the atpyd1-2 mutant in the study. Our findings revealed that the expression of AtNPF6.2/NRT1.4, AtNPF6.3/NRT1.1 and AtNRT2.2 was similarly regulated in atzbip62 and atpyd1-2 but differentially regulated between the mutant lines and Col-0. Meanwhile, the expression pattern of AtNRT2.1 in atbzip62 was similar to that observed in Col-0 but was suppressed in atpyd1-2. The breakthrough is that AtNRT2.2 had the highest expression level in Col-0, while being suppressed in atbzip62 and atpyd1-2. Furthermore, the transcript accumulation of AtGLU1 and AtGLU2 showed differential regulation patterns between Col-0 and atbzip62, and atpyd1-2. Therefore, results suggest that of all tested NO3 transporters, AtNRT2.2 is thought to play a preponderant role in contributing to NO3 transport events under the regulatory influence of AtbZIP62 TF in response to drought stress.

PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.

Environmental acclimation ability plays a key role in plant growth, although the mechanism remains unclear. Here, we determined the involvement of Arabidopsis thaliana PLANT DEFENSIN 1 gene AtPDF1.5 in the adaptation to low nitrogen (LN) levels and cadmium (Cd) stress. Histochemical analysis revealed that AtPDF1.5 was mainly expressed in the nodes and carpopodium and was significantly induced in plants exposed to LN conditions and Cd stress. Subcellular localization analysis revealed that AtPDF1.5 was cell wall- and cytoplasm-localized. AtPDF1.5 overexpression significantly enhanced adaptation to LN and Cd stress and enhanced the distribution of metallic elements. The functional disruption of AtPDF1.5 reduced adaptations to LN and Cd stress and impaired metal distribution. Under LN conditions, the nitrate transporter AtNRT1.5 expression was upregulated. Nitrate transporter AtNRT1.8 expression was downregulated when AtPDF1.5 was overexpressed, resulting in enhanced transport of NO3− to shoots. In response to Cd treatment, AtPDF1.5 regulated the expression of metal transporter genes AtHMP07, AtNRAMP4, AtNRAMP1, and AtHIPP3, resulting in higher Cd accumulation in the shoots. We conclude that AtPDF1.5 is involved in the processing or transmission of signal substances and plays an important role in the remediation of Cd pollution and LN adaptation.

Sensitivity of PM2.5 and O3 pollution episodes to meteorological factors over the North China Plain

The Comprehensive Air-quality Model with extensions (CAMx) was used to explore the sensitivity of PM2.5 and O3 concentrations to four selected meteorological factors: wind speed, temperature, water vapor mixing ratio (Q), and planetary boundary layer height (PBLH) during two pollution episodes over the North China Plain (NCP). We also investigated the impact pathways of different meteorological factors on the formation of PM2.5 and O3. It is found that PM2.5 was more sensitive to the selected meteorological factors in the southeastern NCP, where high anthropogenic emissions and severe air pollution occur. Large variations were observed along the Taihang Mountains, where the height of the terrain changes dramatically. The sensitivity of O3 to wind speed, PBLH, temperature, and Q was mainly determined by the inhibition effects of PM2.5 in winter, while in summer, the complex chemical reactions were dominant. Significant diurnal variations of process analysis (PA) results were observed under various meteorological conditions. Higher temperature generally enhance heterogeneous chemistry and transport of NO3− through the top boundary layer during night-time in winter, however, in summer, the heterogeneous chemistry of NO3− and NH4+ during daytime were the major pathways to the increased PM2.5 due to increased temperature. Moreover, temperature alter PM2.5 concentrations through affecting vertical diffusivity and relative humidity, and alter O3 concentrations by affecting the gas phase chemistry and mass fluxes through the top boundary layer. Q mainly affects the rate of chemical reactions of PM2.5 and O3. The different impact pathways suggest that it is essential to consider variations in meteorological factors, in addition to the direct impacts of wind speed and PBLH, more attention should be paid to the complex impacts of temperature and Q, when developing emission control strategies.

Important factors when simulating the water and nitrogen balance in a tile-drained agricultural field under long-term monitoring

Despite the effectiveness of tile drain systems as a water management practice in naturally poorly drained soils, they facilitate the transport of NO3−-N to surface water bodies. In order to improve the risk assessment of this significant transport under increased applications of N fertilisers in agriculture, it is imperative to delineate the controlling factors and processes. The aim of this study was to acquire such knowledge using the 1D Daisy model to simulate water and N balance based on comprehensive data from a ten-year monitoring study of a tile-drained loamy field in Denmark under the actual crop rotation of winter wheat, sugar beet, spring barley, winter rape and maize. The model simulated the cumulative drainage and NO3−-N leaching over the ten-year period satisfactorily with NSE of 1.00 and 0.87 respectively. While the annual N input to the model was 181 kg N ha−1, an average of 139 kg N ha−1 was harvested in the crop, 22 kg N ha−1 was leached through deep percolation, 17 kg N ha−1 was leached to the tile drains, and 14 kg N ha−1 was lost due to denitrification. Although the model satisfactorily captured the monitored data, the results of this study highlight: (i) the requirement for improved parameterisation of winter crops, (ii) the need to give further consideration in the model to soil surface and macropore processes that govern water infiltration and (iii) that measured and simulated NO3−-N concentrations in the drainage exceeded the limit defined by the European Drinking Water Directive and Nitrates Directive for drinking water and hence improved N management strategies are essential for tile-drained agricultural fields in temperate regions under conventional crop rotations.

Understanding the impact of vehicular emissions on air pollution from the perspective of regional transport: A case study of the Beijing-Tianjin-Hebei region in China

Motor vehicles are recognised as important contributors to urban air pollution. However, few studies have focused on their contribution from the point of view of regional transport. To address this gap, the validated WRF-CAMx-PSAT modelling system was applied to quantitatively evaluate the impact of vehicular emissions on air pollution in the Beijing-Tianjin-Hebei (BTH) region of China in 2014. We found that although motor vehicle is a near-surface source, it can significantly influence the air quality through regional transmission, especially with respect to atmospheric PM2.5 and NO3− during haze days. The fraction of non-local emissions to the overall vehicular emission contribution was 45.2% and 60.3% for PM2.5 and NO3−, respectively. Furthermore, the regional transport of vehicular emissions played an increased significant role in contributing toward air pollution on haze days. The contribution of non-local vehicular emissions to atmospheric PM2.5 and NO3− from non-haze to haze days increased by 38% and 27%, respectively. An 8.01 times change in the per unit (104 ton) vehicular emission contribution (PVEC) of non-local emissions from non-haze to haze days for NO3− indicated that the control of non-local vehicular emissions can be more effective for the mitigation of NO3− pollution, as compared to the mitigation of undifferentiated emissions from different sources. An obvious power function relationship was found between the PVEC and transport distance. Attenuation index was defined to analyse the degree of attenuation of pollutants with distance. An obvious decrease in the attenuation coefficient from non-haze to haze days for NO3− indicated that the regional transport of NO3− was more prominent on haze days from vehicular emission. Thus, this study can provide new knowledge for understanding the environmental impact of vehicular emissions. In addition, the results can provide a scientific basis for formulating effective regional coordination control strategies for air pollution.