Transmission X-ray Microscopy Research Articles

Insights into the effect of various supports on hydrothermal liquefaction of food waste over iron-oxide nano-catalysts

This work investigates the effect of supported iron-oxide nano-catalysts for hydrothermal conversion of food waste. The studied supports were Vulcan carbon (VC), CeO2, ZSM-5 and amorphous SiO2-Al2O3. Catalytic hydrothermal liquefaction experiments were carried out in a batch reactor at 16 MPa and 300 °C maintained for 1 h. Different fractions of Fe(0), Fe2+ and Fe3+ alter its tendency toward deoxygenation, hydrogenations and condensation reactions, which influence the bio-crude yield, elemental compositions, and energy recoveries. The fresh and spent catalysts were characterized using X-ray photoelectron spectroscopy, physisorption analysis, thermogravimetric analysis, transmission and scanning electron microscopy. It was found that the change in catalyst support influences HTL pathways and product compositions. The results reveal that the inclusion of FeOx catalyst on Vulcan carbon, SiO2-Al2O3 and ZSM-5 supports can increase the bio-crude yield by ~7–9 wt% compared to their FeOx-free yields. The increase in bio-crude yield was associated with the decrease in the surface ratios of Fe3+/Fe2+ at the range of 0.8–1.6. In overall, catalysts that had higher tendencies in converting amines into oil-soluble compounds increased the bio-crude yield, while catalysts that promoted dehydration and decarboxylation route decreased the bio-crude yield. The maximum energy recovery in bio-crude was obtained using FeOx/SiO2-Al2O3 catalyst with values ~95 %. The deactivation of catalysts was associated with the increase in Ca and P poisonous elements on catalytic sites, which decreased the energy recovery of recycled FeOx/SiO2-Al2O3 to ~85 % after three cycles.

Microbial Surface Confined Growth Strategy for the Synthesis of Highly Loaded NiCoP Nanoparticles with Hollow Derived Carbon Shells for Sodium Ion Capture.

NiCoP is considered to be a very promising material for sodium ion (Na+) capturing, however, the volume expansion and poor cyclic stability of NiCoP during the storage limit its application. In response to these limitations, Finite element simulations are used to help in the rational design of the NiCoP structure. A novel microbial surface confined growth strategy is employed to synthesize highly loaded NiCoP nanoparticles (NiCoP NPs) supported on hollow derived carbon shells (NPC), constructing a stable composite structure known as NiCoP@NPC. The highly loaded and uniformly dispersed NiCoP NPs are anchored in-situ and fully exposed, enabling enhanced electron and ion transport efficiency and thereby boosting pseudocapacitance. The NPC from yeast played a crucial role in mitigating the volume expansion of NiCoP NPs, thereby enhancing the structural stability of the electrode. Consequently, NiCoP@NPC demonstrated a high Na+ storage capacity of 59.70 ± 1.51 mg g-1 at 1.6 V and maintained good cycling stability, retaining over 73.3% of its capacity after 80 cycles at 1.6 V. Scanning transmission X-ray microscopy (STXM) analysis confirmed the reversible conversion reaction mechanism and the robust structure of NiCoP@NPC before and after the reaction; Density function theory (DFT) and electrochemical quartz crystal microbalance (EQCM-D) further confirmed that the structural design of NiCoP@NPC promoted electron transport, Na+ adsorption as well as improved cycling stability. This study is intended to provide a new idea for the in-situ confined synthesis of metal phosphides electrodes with stable performance and structure.

Phase-separated polymer blends for controlled drug delivery by tuning morphology

Controlling drug release rate and providing physical and chemical stability to the active pharmaceutical ingredient are key properties of oral solid dosage forms. Here, we demonstrate a formulation strategy using phase-separated polymer blends where the morphology provides a route for tuning the drug release profile. By utilising phase separation of a hydrophobic and a hydrophilic polymer, the hydrophilic component will act as a channelling agent, creating a porous network upon dissolution that will dictate the release characteristics. With ptychographic X-ray tomography and scanning transmission X-ray microscopy we reveal how the morphology depends on both polymer fraction and presence of drug, and how the drug is distributed over the polymer domains. Combining X-ray imaging results with dissolution studies reveal how the morphologies are correlated with the drug release and showcase how tuning the morphology of a polymer matrix in oral formulations can be utilised as a method for controlled drug release.

Scanning Transmission Soft X-Ray Microscopy Probes Topical Drug Delivery of Rapamycin Facilitated by Microneedles.

Scanning Transmission X-ray microscopy (STXM) is a sensitive and selective probe for the penetration of rapamycin which is topically applied to human skin ex vivo and is facilitated by skin treatment with microneedles puncturing the skin. Inner-shell excitation serves as a selective probe for detecting rapamycin by changes in optical density as well as linear combination modeling using reference spectra of the most abundant species. The results indicate that mechanical damage induced by microneedles allows this drug to accumulate in the stratum corneum without reaching the viable skin layers. This is unlike intact skin which shows no drug penetration at all and underscores the mechanical impact of microneedle skin treatment. These results are compared to drug penetration profiles of other drugs highlighting the importance of skin barriers. High spatial resolution studies also indicate that the lipophilic drug rapamycin is observed in corneocytes. Attempts in data evaluation are reported to probe rapamycin also in the lipid layers between the corneocytes, which was not accomplished before. These results are compared to recent results on rapamycin uptake in skin where barrier impairment was induced by pre-treatment with the enzyme trypsin and drug formulations leading to occlusion.

Soft X-ray spectromicroscopic proof of a reversible oxidation/reduction of microbial biofilm structures using a novel microfluidic in situ electrochemical device

In situ electrochemistry on micron and submicron-sized individual particles and thin layers is a valuable, emerging tool for process understanding and optimization in a variety of scientific and technological fields such as material science, process technology, analytical chemistry, and environmental sciences. Electrochemical characterization and manipulation coupled with soft X-ray spectromicroscopy helps identify, quantify, and optimize processes in complex systems such as those with high heterogeneity in the spatial and/or temporal domain. Here we present a novel platform optimized for in situ electrochemistry with variable liquid electrolyte flow in soft X-ray scanning transmission X-ray microscopes (STXM). With four channels for fluid control and a modular design, it is suited for a wealth of experimental conditions. We demonstrate its capabilities by proving the reversible oxidation and reduction of individual microbial biofilm structures formed by microaerophilic Fe(II)-oxidizing bacteria, also known as twisted stalks. We show spectromicroscopically the heterogeneity of the redox activity on the submicron scale. Examples are also provided of electrochemical modification of liquid electrolyte species (Fe(II) and Fe(III) cyanides), and in situ studies of electrodeposited copper nanoparticles as CO2 reduction electrocatalysts under reaction conditions.

Unraveling chemical origins of dendrite formation in zinc-ion batteries via in situ/operando X-ray spectroscopy and imaging

To prevent zinc (Zn) dendrite formation and improve electrochemical stability, it is essential to understand Zn dendrite growth, particularly in terms of morphology and relation with the solid electrolyte interface (SEI) film. In this study, we employ in-situ scanning transmission X-ray microscopy (STXM) and spectro-ptychography to monitor the morphology evolution of Zn dendrites and to identify their chemical composition and distribution on the Zn surface during the stripping/plating progress. Our findings reveal that in 50 mM ZnSO4, the initiation of moss/whisker dendrites is chemically controlled, while their continued growth over extended cycles is kinetically governed. The presence of a dense and stable SEI film is critical for inhibiting the formation and growth of Zn dendrites. By adding 50 mM lithium chloride (LiCl) as an electrolyte additive, we successfully construct a dense and stable SEI film composed of Li2S2O7 and Li2CO3, which significantly improves cycling performance. Moreover, the symmetric cell achieves a prolonged cycle life of up to 3900 h with the incorporation of 5% 12-crown-4 additives. This work offers a strategy for in-situ observation and analysis of Zn dendrite formation mechanisms and provides an effective approach for designing high-performance Zn-ion batteries.

Synergistic ultra-high adsorption and oxidation of arsenic in groundwater by iron-modified biochar: Mechanisms and potential application

This study explores the potential of low-cost biochar modified with ferrihydrite and goethite for the remediation of heavily As-polluted groundwater. Combining batch experiments and synchrotron radiation-based characterization, we investigated the mechanisms of As(III) adsorption and oxidation. The modified biochars exhibited significant adsorption capacities, achieving fitted qmax of 45.7 ± 3.9 mg/g for ferrihydrite-modified biochar (FhGM) and 20.2 ± 1.5 mg/g for goethite-modified biochar (GtGM) at a dosage of 1.0 g/L and an initial concentration of 10 mg/L As(III) over 24 h. Biochar facilitated approximately 30 % As(V) generation from As(III) due to enhanced electron transfer. Moreover, over 90 % of As(III) was oxidized to As(V) following the addition of H2O2, with scanning transmission X-ray microscopy revealing the distribution of As(III), and As(V) on the solid phase. In the GtGM-H2O2 system, reactive oxidation species, primarily O2•–, served as the main oxidant. In the FhGM-H2O2 system, Fe(IV) likely acted as the primary oxidizing agent. Column experiments with 0.64 g of FhGM demonstrated that, when treating 50 mg/L As-contaminated groundwater, the effluent total As concentration remained below the maximum allowable limit of 10 μg/L after 21 h. Furthermore, in the presence of H2O2, the system managed to treat 200 mg/L As-contaminated groundwater, and the effluent total As concentration exceeded 10 μg/L after 11 h. This study provides valuable insights and practical results for the remediation of high-concentration arsenic-contaminated groundwater, highlighting the effectiveness of iron-modified biochar in this application.

Comparison of soft X-ray spectro-ptychography and scanning transmission X-ray microscopy

Over the past decade advances in instrumentation and software have enabled development of spectro-ptychography (SP) as a higher spatial resolution extension of scanning transmission X-ray microscopy (STXM). Direct comparisons are made of same-area chemical state imaging of Cu nanoparticles using STXM and SP in order to compare and contrast the two approaches. We show that SP gives very similar chemical state information as STXM with significantly better spatial resolution and much higher quality images and chemical maps, on account of finer pixels in the reconstructed images. When defocused spot sizes are used (i.e., 1–3 μm, as opposed to full-focus 30–50 nm) SP data acquisition is faster and the radiation dose delivered to the sample is smaller than the corresponding STXM measurement. The limitations of SP are primarily related to the time and complexity of the ptychographic reconstruction. We argue that these documented advantages mean that SP rather than STXM should be used for more complex studies such as tomography and in situ studies, especially when radiation damage is a concern. The main point of this manuscript is to illustrate, with scientifically relevant samples, the significant advantages of SP relative to conventional STXM, with the goal of encouraging greater use of SP.

Reduced CO2 Release from Arctic Soils Due to CO2 Binding to Calcium Forming Aragonite.

Arctic soils are the largest pool of organic carbon compared with other soils globally and serve as a main source for greenhouse gases, especially in the course of the predicted future temperature increase. With increasing temperatures, substantial thawing of the permafrost layer of soils is expected, altering the availability of calcium in those soils, with an increase by ∼5 mg Ca g-1 DW predicted for Alaska. Here we show for two representative soils in Alaska (initially Ca-poor or Ca-rich) that this increase in Ca availability will lead to decreases in CO2 release by 50% and 57%. It is already well-known that the cation bridging of Ca ions to organic carbon renders this carbon unavailable for microbial respiration and that Ca is altering the transformation of Corg by microbes. Here we show that the decrease of the soil CO2 release may be also due to enhanced aragonite formation (by 300% for Ca-poor and 90-200% for Ca-rich soils), as revealed by synchrotron-based scanning transmission X-ray microscopy. We therefore call upon field experiments for validation of this process and inclusion of this process in global and local carbon budget models.

Investigation of the spatial distribution of sodium in bread microstructure using X-ray, light and electron microscopy

The sodium consumption in many countries is too high, which results in increased risk for hypertension, cardiovascular diseases, stroke and premature death. Inhomogeneous sodium distribution using layering is a viable way to reduce sodium in bread that normally contains a lot of sodium. Prevention of sodium migration during production and storage is important for the function of this approach. Furthermore, the distribution of sodium between starch and gluten influences their properties. The spatial distribution of sodium was investigated at high resolution using combinations of X-ray fluorescence microscopy (XFM), scanning transmission X-ray microscopy (STXM), light microscopy (LM), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) and image analysis. Reference breads and layered bread samples were baked with one salt-free layer and one layer containing 3.6 wt% sodium chloride salt. The obtained results showed that the concentration of sodium is higher in the starch phase than in the gluten phase and that sodium migrates across the layer interface from the salt-containing to the salt-free layer. The ratios between the sodium concentration in the starch and gluten phases were dependent on the sodium concentration across the interfaces. Furthermore, magnesium and phosphor signals in bread yeast cells were observed using XFM.

Enhanced thermoelectric properties of Ag2Se by manipulation in carrier concentration via acetylene carbon black nanocomposites

Pristine Ag2Se has inherent limitations in its Seebeck coefficient and electronic thermal conductivity, necessitating improvements through carrier concentration manipulation. While its porous structure has been utilized to reduce carrier concentrations, achieving optimal values remains challenging. This study explores enhancing Ag2Se with acetylene carbon black (ACB) nanocomposites to further reduce carrier concentrations and improve thermoelectric (TE) properties. ACB, a cost-effective alternative to other carbon materials, has demonstrated potential in enhancing TE performance in various composites. Ag2Se and ACB composites were synthesized by dispersing different ACB weights in a solution, followed by sintering. Characterization using x-ray diffraction (XRD), Transmission and scanning electron microscopy (TEM and SEM), and other techniques confirmed the phase, structure, and morphology of the composites. The addition of ACB resulted in decreased electrical conductivity and increased Seebeck coefficient in Ag2Se, particularly at ACB concentrations up to 5.0 wt%, balancing the power factor (PF). The total thermal conductivity decreased due to mainly reduced electronic thermal conductivity. The 5.0 wt% ACB sample achieved a zT of approximately 0.8 at 383 K, comparable to the performance of composites using more expensive low-dimensional carbon materials.

Novel sensitive electrochemical detection of paracetamol using magnetite/MXene electrode by differential pulse voltammetry

In this research work, a novel electrochemical sensor-based Fe3O4/Ti3C2 MXene @ glassy carbon electrode (GCE) for the detection of paracetamol was prepared by simple ultrasonic method by combining Ti3C2 MXene and Fe3O4 nanoparticles. The Fe3O4/Ti3C2 MXene nanomaterial was characterized by the means of different techniques: X-ray diffraction (XRD), transmission and scanning electron microscopy (TEM), nitrogen adsorption/desorption isotherms, as well as Fourier transform infrared spectroscopy (FTIR). The characterization results revealed the homogenous distribution of the prepared cubic Fe3O4 NPs within the prepared two-dimensional layered Ti3C2 MXene. The Fe3O4/Ti3C2 MXene @ GCE was used for the sensitive determination of the well-known analgesic drug paracetamol by differential pulse voltammetry (DPV). The prepared Fe3O4/Ti3C2 MXene @ GCE was characterized electrochemically, and the results showed that Fe3O4/Ti3C2 MXene @ GCE is a potential working electrode for the sensitive detection of paracetamol with very low detection limit (0.63 nM), within a linear dynamic range of 0.0–110.0 µM, high reproducibility and repeatability; with a very low relative standard deviation (SD%) for 7 days measurement (3.07–3.42 %), accuracy with a SD% of 0.89, high precision of 99.48 % in distilled water and 98.51 % in sea water. The proposed electroanalytical method was applied for the detection and quantification of paracetamol in real water samples, as well as different analgesic medical formulations, and the results showed outstanding accuracy and precision indicating the suitability of the Fe3O4/Ti3C2 MXene @ GCE electrode for the sensitive, accurate determination of paracetamol in diferrent matrices.

Energy storage chemistry: Atomic and electronic fundamental understanding insights for high-performance supercapacitors

The scarcity of fuels, high pollution levels, climate change, and other major environmental issues are critical challenges that modern societies are facing, mostly originating from fossil fuels-based economies. These challenges can be addressed by developing green, eco-friendly, inexpensive energy sources and energy storage devices. Electrochemical energy storage materials possess high capacitance and superior power density. To engineer highly efficient next-generation electrochemical energy storage devices, the mechanisms of electrochemical reactions and redox behavior must be probed in operational environments. They can be studied by investigating atomic and electronic structures using in situ x-ray absorption spectroscopy (XAS) analysis. Such a technique has attracted substantial research and development interest in the field of energy science for over a decade. The mechanisms of charge/discharge, carrier transport, and ion intercalation/deintercalation can be elucidated. Supercapacitors generally store energy by two specific mechanisms—pseudocapacitance and electrochemical double-layer capacitance. In situ XAS is a powerful tool for probing and understanding these mechanisms. In this Review, both soft and hard x rays are used for the in situ XAS analysis of various representative electrochemical energy storage systems. This Review also showcases some of the highly efficient energy and power density candidates. Furthermore, the importance of synchrotron-based x-ray spectroscopy characterization techniques is enlightened. The impact of the electronic structure, local atomic structure, and electronically active elements/sites of the typical electrochemical energy storage candidates in operational conditions is elucidated. Regarding electrochemical energy storage mechanisms in their respective working environments, the unknown valence states and reversible/irreversible nature of elements, local hybridization, delocalized d-electrons spin states, participation of coordination shells, disorder, and faradaic/non-faradaic behavior are thoroughly discussed. Finally, the future direction of in situ XAS analysis combined with spatial chemical mapping from operando scanning transmission x-ray microscopy and other emerging characterization techniques is presented and discussed.

From irregular to regular eutectic growth in the Al-Al3Ni system: In situ observations during directional solidification

We investigate the irregular eutectic growth dynamics of the Al-Al3Ni alloy, in which one of the solid phases (Al3Ni) grows faceted from the liquid. Leveraging in situ optical microscopy and synchrotron transmission x-ray microscopy, we address the question of the degree of coupling between Al and Al3Ni at the growth front and that of the shape of the microstructures left behind in the bulk solid during directional solidification. Real-time optical observations bring evidence for a morphological transition from a eutectic-grain dependent, irregular eutectic growth at low solidification velocity V (typically 1μms−1), to a weakly anisotropic, regular growth at higher V (reaching 10μms−1). Unprecedented x-ray nano-imaging of the solid–liquid interface, and 3D characterization of the growth patterns, were made possible by a new DS setup at Brookhaven National Laboratory’s NSLS-II. At low V, the leading tips of partly faceted Al3Ni crystals are observed to grow not far ahead of the Al growth front. Correlating in situ images and postmortem 3D tomographic reconstructions reveals that the presence of faceted and non-faceted regions of Al3Ni crystals in the solid is a direct consequence of coupling and decoupling during DS, respectively. Upon increasing V, the lead distance of Al3Ni vanishes, and the shape of Al3Ni ceases to be governed by faceted growth. These observations cast light onto the basic mechanisms (faceted growth, diffusive coupling, and the dynamics of trijunctions) governing a faceted to rod-like transition upon increasing V in the Al3Ni system, with broad implications to a large class of irregular eutectics.

Microstructure development of calcium carbonate cement through polymorphic transformations

This study presents the mechanical properties and polymorphic transformation of pastes made with calcium carbonate cement, where vaterite was the main component. Both laboratory (compression tests, XRD, & SEM) and synchrotron-based techniques (transmission X-ray microscopy and microtomography) were used in the characterization of the pastes. Mechanical properties were developed by controlling the polymorphic transformation of vaterite. When converting to aragonite, calcium carbonate cement exhibited three times greater strength development compared to its conversion to calcite at a water-to-cement ratio of 0.4, which resulted in 42 % porosity. During the conversion to aragonite, the calcium carbonate cement paste develops an interpenetrating matrix of aragonite needles that enhance strength development through interlocking at a low paste bulk density of 1.6 g/cm3. Together the environmental and mechanical properties of calcium carbonate cement suggest its potential as a greener building material compared to traditional Portland cement.

Nanoscale synchrotron x-ray analysis of intranuclear iron in melanised neurons of Parkinson’s substantia nigra

Neuromelanin-pigmented neurons of the substantia nigra are selectively lost during the progression of Parkinson’s disease. These neurons accumulate iron in the disease state, and iron-mediated neuron damage is implicated in cell death. Animal models of Parkinson’s have evidenced iron loading inside the nucleoli of nigral neurons, however the nature of intranuclear iron deposition in the melanised neurons of the human substantia nigra is not understood. Here, scanning transmission x-ray microscopy (STXM) is used to probe iron foci in relation to the surrounding ultrastructure in melanised neurons of human substantia nigra from a confirmed Parkinson’s case. In addition to the expected neuromelanin-bound iron, iron deposits are also associated with the edge of the cell nucleolus. Speciation analysis confirms these deposits to be ferric (Fe3+) iron. The function of intranuclear iron in these cells remains unresolved, although both damaging and protective mechanisms are considered. This finding shows that STXM is a powerful label-free tool for the in situ, nanoscale chemical characterisation of both organic and inorganic intracellular components. Future applications are likely to shed new light on incompletely understood biochemical mechanisms, such as metal dysregulation and morphological changes to cell nucleoli, that are important in understanding the pathogenesis of Parkinson’s.

Effect of Oxidation on Vivianite Dissolution Rates and Mechanism.

The interest in the mineral vivianite (Fe3(PO4)2·8H2O) as a more sustainable P resource has grown significantly in recent years owing to its efficient recovery from wastewater and its potential use as a P fertilizer. Vivianite is metastable in oxic environments and readily oxidizes. As dissolution and oxidation occur concurrently, the impact of oxidation on the dissolution rate and mechanism is not fully understood. In this study, we disentangled the oxidation and dissolution of vivianite to develop a quantitative and mechanistic understanding of dissolution rates and mechanisms under oxic conditions. Controlled batch and flow-through experiments with pristine and preoxidized vivianite were conducted to systematically investigate the effect of oxidation on vivianite dissolution at various pH-values and temperatures. Using X-ray absorption spectroscopy and scanning transmission X-ray microscopy techniques, we demonstrated that oxidation of vivianite generated a core-shell structure with a passivating oxidized amorphous Fe(III)-PO4 surface layer and a pristine vivianite core, leading to diffusion-controlled oxidation kinetics. Initial (<1 h) dissolution rates and concomitant P and Fe release (∼48 h) decreased strongly with increasing degree of oxidation (0-≤ 100%). Both increasing temperature (5-75 °C) and pH (5-9) accelerated oxidation, and, consequently, slowed down dissolution kinetics.

Understanding the Growth of Electrodeposited PtNi Nanoparticle Films Using Correlated In Situ Liquid Cell Transmission Electron Microscopy and Synchrotron Radiation.

Electrodeposition is a versatile method for synthesizing nanostructured films, but controlling the morphology of films containing two or more elements requires a detailed understanding of the deposition process. We used liquid cell transmission electron microscopy to follow the electrodeposition of PtNi nanoparticle films on a carbon electrode during cyclic voltammetry. These in situ observations show that the film thickness increases with each cycle, and by the fourth cycle, branched and porous structures could be deposited. Synchrotron studies using in situ transmission X-ray microscopy further revealed that Ni was deposited in the oxide phase. Ex situ studies of bulk electrodeposited PtNi nanoparticle films indicated the number of cycles and the scanning rate were the most influential parameters, resulting in a different thickness, a different homogeneity, a different nanoparticle size, and a different surface structure, while the precursor concentration did not have a significant influence. By varying the potential range, we were able to obtain films with different elemental compositions.

Dual-Salt aqueous electrolyte for enhancing Charge-Storage properties of VO2 polymorphic cathodes for Zn-Ion batteries

Three VO2 polymorphs, namely monoclinic VO2 (B), monoclinic VO2 (M), and tetragonal VO2 (A), are synthesized, and their microstructures and electrochemical properties are studied for application in Zn-ion batteries (ZIBs). A cost-effective ZnSO4-based aqueous electrolyte with various concentrations (3 − 7 m) of lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) additive is developed. The optimal ZnSO4/LiTFSI ratio in the electrolyte for superior Zn//VO2 battery properties is examined. The coordination structures of various electrolytes are investigated using wide-angle X-ray scattering. The incorporation of LiTFSI impairs the hydrogen-bonded network of H2O molecules and causes solvated-TFSI and TFSI‧‧‧TFSI structures to form, altering the electrolyte properties (such as electrochemical stability window, ionic conductivity, and Zn(OH)2)3(ZnSO4)·xH2O byproduct formation tendency). It is found that an excessive LiTFSI concentration leads to the formation of ion aggregates in the electrolyte and thus deterioration of electrode specific capacity and rate capability. Operando transmission X-ray microscopy observations confirm the high dimensional stability of the VO2 cathode during charging and discharging; the length variation of the VO2 (B) rods is approximately 10 %. The electrolyte composition also affects the Zn2+/Zn redox behavior at the anode side. The incorporation of LiTFSI into the electrolyte affects the Zn plating/stripping Coulombic efficiency, morphology of the deposited Zn, dead Zn amount, and anode cycle life. The constructed Zn//VO2 (B) cell with 1 m ZnSO4/5 m LiTFSI dual-salt electrolyte shows 90 % capacity retention after 1000 cycles. The proposed electrode material and electrolyte composition have great potential for applications in high-performance and high-stability rechargeable ZIBs.

Protein Corona-Directed Cellular Recognition and Uptake of Polyethylene Nanoplastics by Macrophages.

The widespread use of plastic products in daily life has raised concerns about the health hazards associated with nanoplastics (NPs). When exposed, NPs are likely to infiltrate the bloodstream, interact with plasma proteins, and trigger macrophage recognition and clearance. In this study, we focused on establishing a correlation between the unique protein coronal signatures of high-density (HDPE) and low-density (LDPE) polyethylene (PE) NPs with their ultimate impact on macrophage recognition and cytotoxicity. We observed that low-density and high-density lipoprotein receptors (LDLR and SR-B1), facilitated by apolipoproteins, played an essential role in PE-NP recognition. Consequently, PE-NPs activated the caspase-3/GSDME pathway and ultimately led to pyroptosis. Advanced imaging techniques, including label-free scattered light confocal imaging and cryo-soft X-ray transmission microscopy with 3D-tomographic reconstruction (nano-CT), provided powerful insights into visualizing NPs-cell interactions. These findings underscore the potential risks of NPs to macrophages and introduce analytical methods for studying the behavior of NPs in biological systems.