Transmission Of Infection Research Articles

Rapid Genomic Characterization of High-Risk, Antibiotic Resistant Pathogens Using Long-Read Sequencing to Identify Nosocomial

Background: Current epidemiological methods have limitations in identifying transmission of bacteria causing healthcare-associated infections (HAIs). Recent whole genome sequencing (WGS) studies found that genetically related strains can cause HAIs without meeting standard epidemiologic definitions, but these results could not provide data in a timely fashion needed for intervention. Given recent advances in Oxford Nanopore Technologies (ONT) sequencing, we sought to establish a validated ONT pipeline capable of providing accurate WGS-based comparisons of clinical pathogens within a short time frame that would allow for infection control interventions. Method: Using electronic medical record data, we identified potential healthcare acquisition of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and carbapenem-resistant gram-negative rods. Bacterial genomic DNA was directly extracted from clinical microbiology lab plates. Sequencing was conducted with the ONT MinION sequencer and R10.4.1 flow cell. MINTyper for single nucleotide polymorphism (SNP) calling and Ridom SeqSphere+ for core genome MLST were used to determine genetic relatedness. The main outcome was time from pathogen identification to completed genetic analysis. Result: The weekly workflow, from genomic DNA extraction to complete data analysis, averaged 2.6 days with a standard deviation of 1.3 days. (range: 1 to 6 days). Starting in August 2023, we have sequenced a total of 177 bacterial isolates from 156 unique patients. Isolates came from blood (38%), tissue/wound/body fluid (24%), urinary tract (20%), respiratory tract (16%), and rectal swab (2%). To date, six genetically related clusters have been identified. Three clusters involved ST117 vancomycin-resistant Enterococcus faecium (VREfm), comprising a total of 13 unique patients distributed as 2, 3, and 8 patients in each group, with pairwise SNP differences of 20, 11, and 14. Patients within the same clusters showed epidemiological links through overlapping admissions and temporally shared ICU stays. Additionally, another cluster consisted of five genetically related ST633 Pseudomonas aeruginosa isolates, with a pairwise SNP difference of 57.5. Each patient in this cluster had potential epidemiological links through overlapping admission times, despite the absence of identified shared spaces. The last two clusters involved Klebsiella pneumoniae and Escherichia coli (two cases each), with pairwise SNP differences of 18 and 9, respectively. In both cases, each patient showed potential epidemiological links through overlapping admission times. Conclusion: Our stand-alone ONT pipeline was able to rapidly and accurately detect genetically related AMR pathogens, aligning closely with epidemiological data. Our approach has the potential to assist in the efficient detection and deployment of preventative measures against healthcare-associated infection transmission.

Serological markers of transfusion transmissible infections and ABO blood groups in Najran, Saudi Arabia.

To ascertain the prevalence of transfusion transmissible infections (TTIs) across diverse donor groups in the Najran province. Additionally, to establish a potential association between the development of TTI and the donors' blood group, as determined by the ABO/Rh blood grouping system. Blood donation data of 4120 donors, spanning from January to December 2020, were retrospectively reviewed. The blood were screened for TTI markers, including hepatitis B surface antigen (HBsAg), anti-hepatitis B core (anti-HBc), anti-hepatitis C virus (anti-HCV), anti-human immunodeficiency viruses 1 and 2 (anti-HIV1&2), anti-human T-lymphotropic virus types 1 and 2 (anti-HTLV-1&2), and syphilis antigen. Positive TTI markers were detected in 10.9% of the donors. The most detected TTI marker was anti-HBc (8.9%), followed by HBsAg (0.7%). Other markers were individually detected in <1% of the donors. Anti-HBc-positive was significantly elevated among non-Saudi blood donors. There was an association between age groups and anti-HCV (p=0.002), anti-HTLV (p=0.004) and syphilis antigen (p=0.02) markers positivity. The AB positive blood group exhibited the most positivity for TTI markers, followed by O positive blood group. Similarly, association was found between ABO group and HBsAg (p=0.01), anti-HBc (p=0.001), and anti-HCV (p<0.001) markers positivity. Emphasis on implementing robust screening measures for donated blood is underscored by this study. There is the need for future study to extensively evaluate TTI status to enhance our understanding of the trend in TTI.

Unveiling Leptospira Antibody Seroprevalence among Voluntary Blood Donors: Insights from a Single-center Observational Study

Abstract BACKGROUND: Leptospirosis is among transfusion transmissible infections but is not screened routinely among blood donors and is endemic in Southern India. The presence of carrier state and the donors being asymptomatic may lead to transfusion transmission in the absence of screening. We aimed to study the prevalence of leptospirosis among blood donors and to assess the need to implement screening strategies for its detection. METHODS: A prospective observational study was conducted among blood donors using Purposive sampling to select donors for the study. The samples collected for routine transfusion transmissible infections screening were used to screen for Ig M antibodies to leptospira infection by Enzyme linked immunosorbent assay (ELISA) method. Samples that were reactive twice were true positive. These donors were contacted telephonically to enquire if they developed any symptoms of the infection. RESULTS: A total of 3576 came for blood donation during the study period of which 124 participated in the study. Among the 124 donors screened, a total of seven samples (5.64%) samples were found to be reactive for IgM Leptospira antibodies. The mean age of the donors that were found to be reactive was 27.5 years (SD: 3.15, Range: 20-45 years) with a male to female ratio of 6:1. All these donors were asymptomatic before or after seven days and 2 weeks of blood donation. CONCLUSION: Approximately six out of 100 donors screened were positive for IgM antibodies of Leptospira without any symptoms. As there is a risk of transmission via blood transfusion, regular screening may be needed at least in areas of high prevalence.

Sexual risk and testing for sexually transmissible infections in Aboriginal and Torres Strait Islander peoples and non-Indigenous young South Australians: results of an online survey.

Background Disproportionate rates of sexually transmissible infections (STIs) among Aboriginal and Torres Strait Islander young people are often attributed to risk-taking behaviours, but research rarely conducts direct comparison with their non-Indigenous peers to address this negative discourse. Methods 'Let's Talk About It 2019' was a cross-sectional online survey of South Australians (16-29 years). It prioritised recruitment of Aboriginal and Torres Strait Islander respondents to compare behaviours with non-Indigenous peers using multivariable Poisson regression models. Results Aboriginal and Torres Strait Islander (n =231) and non-Indigenous (n =2062) respondents reported similar condom use (40% vs 43%, P =0.477) and sexual debut median ages (16 years vs 17 years). Higher proportions of Aboriginal and/or Torres Strait Islander respondents reported a recent health check (48% vs 38%, P =0.002), STIs (60% vs 49%, P P =0.006) testing, STI diagnosis (29% vs 21%, P =0.042), and intoxication during last sex (30% vs 18%, P Conclusions Behaviours associated with STI transmission were mostly similar among Aboriginal and Torres Strait Islander and non-Indigenous respondents. Higher STI/HIV testing among Aboriginal and Torres Strait Islander respondents suggests effectiveness of targeted programs. Interventions targeting substance use and condom use among all young people are needed. Future interventions need to focus beyond behaviours and explore social determinants of health and sexual networks as contributors to disproportionate STI rates.

INTRA-FAMILIAL TRANSMISSION OF HEPATITIS B VIRUS IN ADEN -YEMEN

Hepatitis B virus (HBV) is a major challenge to public health worldwide. Close contacts between susceptible family members and the index cases who live with HBV infection increase the rate intra-familial transmission of HBV. So our study aimed to determine the prevalence of HBV infection among family members of confirmed positive HBV patients and to identify the intra and extra-familial risk factors contributing for transmission of HBV infection among those members. Analytical cross-sectional study was conducted on 100 family members who belong to the 14 index cases were included in this study, the average was 7.1(100/14) members. HBsAg in both index cases and family members was determined by using ELISA kit. Analysis of the data was performed by using SPSS (Version 21), the quantitative data were expressed as mean values, (SD) because the data was normal distributed. The qualitative data was expressed as percentages and χ2 test was used for comparison of two variables to determine the p- value where &lt;0.05 was considered statistically significant. They were 50 (50 %) males with a total mean± SD of age were 25±15.9 years. The overall prevalence of HBV infection was 19 (19. 0%). The highest rates were 41.2% in age group 30—39 years, 22% males, 26.7% married, 19.4% among those family members who were in close contact with female index cases and 40% father relatives. A statistically significance association between HBV infection and age group 30—39 years (p=0.011). There were no positive cases was found among those members who were vaccinated against HBV and no significant association between HBV infection and either intra or extra risk factors among family members. It can be concluded that Intra-familial transmission of HBV infection was high among family member in Yemen. The highest prevalence was found in age group 30-39 years, males, married and among those family members who were in close contact with female index cases. The most frequent relatives with HBV infection were fathers, while a negative HBV cases was observed among mothers and wives and vaccinated members. There were no significant association between HBV infection and either intra or extra risk factors among family members.

Tato sebagai Metode Identifikasi Korban Meninggal Tanpa Identitas

The identification of individuals is a critically important aspect of the forensic examination process, as errorsin the identification process can have fatal consequences in the legal proceedings. The identification of a living patientcan be carried out through various methods, including fingerprint identification, visual recognition, documentation,accessories, dental records, serological examinations, and biomolecular analysis. Presented is a case study of a maleindividual who died as a result of a motor vehicle accident involving a bus, which resulted in head and chest trauma,leading to the victim's immediate demise at the scene. No identifying marks were found on the victim, but the victimhad a tattoo on the left chest. Identification and external examinations were conducted by a team of doctors andINAFIS personnel, synchronized with the information provided by the victim's family. Subsequently, an analysis wasperformed, correlating with a review of the literature based on scientific publications. The final result was thesuccessful identification of the deceased individual, thus concluding that tattoos can be used as one of the methods ofidentification for individuals or unidentified bodies, supported by other methods such as family data and other uniqueindividual characteristics. However, the use of tattoos needs to be handled with caution due to the risk of infection,allergies, or the transmission of infections through poorly executed and unclean tattoo procedures.

Bacteriological and Mycological Assessments of Second-Hand Wears Procured From Girei And Jimeta Markets, Adamawa State, Nigeria

Study’s Novelty/Excerpt This study investigates into the microbial contamination of second-hand clothes, highlighting their potential role in infection transmission. The research highlights the significant phosphate-solubilizing activity of these native strains, which enhances phosphorus availability to plants through the secretion of organic acids and enzymes. The findings underscore the need for public health interventions, including the washing and disinfection of second-hand clothing to mitigate the risk of spreading antibiotic-resistant pathogens. Full Abstract Used clothes can potentially be an element in the chain of infection transmission during normal daily activities. Second-hand wear is among such used clothes purchased by many people and used directly without considering any attending health implications. This study, therefore, examined the level of microbial contamination of second-hand clothes and the association between the different categories of the clothes and microbial contaminants. Second-hand clothes such as shirts, trousers, vests, socks, and shoes were bought from three Markets in Girei, Yola North and Yola South. Isolation of organisms was carried out via Standard Plate Count. Isolates were identified using morphology and appropriate biochemical methods. A chi-square test (P ≤ 0.05 significant) was done to analyze some of the data obtained. Antibiotic susceptibility of the bacterial isolates was also determined using the Kirby-Bauer method. The most frequently isolated bacteria were Staphylococcus aureus. Other bacteria isolated were Pseudomonas spp and E. coli. A total of four fungal species were isolated. These are Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger and Trichophyton rubrum. Trousers had the highest bacterial count (1.10 x103 CFU/mL) and fungal count (8.00 x104 CFU/mL). The data obtained shows no significant association between fungal species and the categories of clothes with (P = 0.15), but there is a significant association between bacterial species isolated and the categories of clothes analyzed (P = 0.02). S. aureus was susceptible to ciprofloxacin (100%) and erythromycin (92.8%), while Pseudomonas sp and E. coli were most susceptible to ciprofloxacin and sparfloxacin (100%). The least susceptibility was observed with amoxicillin and sulfamethoxazole-trimethoprim. It became obvious that these clothes are possible carriers of antibiotic-resistant pathogens for skin and other infections. Washing and disinfecting second-hand wear before use may be important in minimizing the potential health implications associated with the isolated pathogens.

Evaluation of T. gondii, rubella, and cytomegalovirus seroprevalences among female Syrian refugees in Sanliurfa, Turkiye.

Since the Syrian Civil War began in 2011, the official number of refugees under temporary protection in Turkiye is reported to be 3,522,036 in 2023. Most of the Syrians living outside the refugee camps have worse conditions in terms of access to healthcare centers and social opportunities, compared to those living in camps. The Sanliurfa province hosts the third highest number of Syrians (370,291) in Turkiye. There are no data about the seroprevalence of Toxoplasma gondii (T. gondii), rubella (rub), or cytomegalovirus (CMV) among Syrian refugees in Sanliurfa. We aimed to investigate the seroprevalence of T. gondii, rub, and CMV infections among female Syrian refugees of reproductive age (15-49 years) living in Sanliurfa province. A cross-sectional study was conducted in different districts of Sanliurfa. A total of 460 households were selected using the probability sampling method. One married female Syrian refugee aged between 15 and 49 years, was chosen in each household, leading to a sample size of 410 female Syrian refugees. The seropositivity of T. gondii, CMV, and rub IgM and IgG in blood samples were analyzed using enzyme immunoassays (Abbott Architect, Illinois, USA). The seropositivity rates of T. gondii, CMV, and rubella IgM and IgG were 4.4% and 59.8%; 3.9%; and 99%; and 1.9%, and 99.5%, respectively. A screening program should be implemented for T. gondii, CMV, and rub infections for Syrian refugees. Seronegative women should be vaccinated against rub and educated about the transmission and preventive routes of toxoplasmosis and CMV infection.

Serological and molecular survey of Q fever in the dog population of the Campania region, southern Italy

Q fever is a re-emerging zoonosis whose epidemiological cycle in ruminants is well defined, while the role of other species (including pets) is still debated. In this study, the serological and molecular prevalence of Coxiella burnetii in a sample of dogs in the Campania region, southern Italy was evaluated. A seroprevalence of 5.97 % (16/268) was observed using a commercial multispecies ELISA, compared to only 2.7 % (5/197) at the molecular level. No risk factors correlated with higher levels of exposure except for the size of the animal (small dogs showed significantly higher seroprevalence). Positive samples were further evaluated for reactivity to phase I and II antigens using IFA and phase-specific ELISAs (for specific IgG detection). Two animals showed antibodies against both phases of infection, suggesting that Coxiella burnetii seroconversion in dogs follows similar dynamics to those observed in ruminants.One of the five samples that showed positive results in real-time PCR was confirmed at the PCR endpoint and showed similarity with other Coxiella spp. strains detected in tick and dog samples when sequenced.In this study, we demonstrated exposure to Coxiella burnetii for different categories of dogs in southern Italy, including pet dogs living indoors. Since reports of transmission of infection from pets to humans have been described in both rural and urban areas, careful surveillance of these species is also necessary. In the lack of additional information, comprehending the risk to humans requires monitoring of wild and domestic animal populations.

Sheath Blight and Bacterial Blight Resistance in Rice: Mechanisms, Progress and Future Perspectives for Sustainable Rice Production

Rice (Oryza sativa L.) is a staple food for nearly half of the world's population, including Pakistan. But several diseases are always posing a threat to rice farming, with bacterial blight and sheath blight being the two most common causes. R. solani is the main source of rice sheath blight and stood crop’s most destructive diseases. Reviewing published data on pathogenicity and disease management is crucial to developing effective crop protection against sheath blight. It also helps identify research gaps that need more in-depth investigation. Although there has been progress in identifying rice and pathogen-related genes linked to pathogenesis, the underlying processes are still unknown. Research on the applications of; agronomic techniques, chemical control, biological control, and genetic improvement in disease management strategies has been conducted. Optimizing the application of nitrogen fertilizer in combination to plant-plant spacing can minimize the transmission of infection, whereas SMART agricultural technologies; like crop monitoring using Unmanned Aerial Systems help detect and treat sheath blight disease early on. Biological agents and natural fungicides can be used to effectively prevent sheath blight while reducing the negative effects on the environment. Genetic strategies that hold potential to control sheath blight include the use of exogenous dsRNA to suppress pathogen gene expression, genome editing to create rice lines less susceptible to sheath blight, and the development of transgenic rice lines that over express or silence genes related to pathogenesis. The pathogen’s flexibility, the absence of resistant rice types, the lack of single resistance genes for breeding, and farmers' restricted access to informative programs about optimum management methods all work against effective crop protection against sheath blight.

Advancements in Fish Vaccination: Current Innovations and Future Horizons in Aquaculture Health Management

Aquaculture is rapidly becoming one of the pivotal sectors in the farming economy, driven by the increasing demand for high-quality animal protein at an affordable cost, especially with the escalating human population. However, the expansion of high-density fish populations also brings forth a challenge—the rapid transmission and spread of infectious disease agents among them. To combat this, vaccination is emerging as a reliable and standardized method for providing immunity against viral and bacterial outbreaks. The ideal vaccine is expected to be safe, effective, economical, and easily administered. The fish vaccination industry continually publishes new information on fish immunology and vaccinology, contributing to the improvement in vaccine formulation and efficacy. This review aims to offer insights into the current status of bacterial, viral, and parasitic diseases, discuss existing vaccinations, and address potential industry-threatening diseases like infectious edwardsiellosis, motile aeromonas septicemia (MAS), Tilapia Lake Virus (TiLV) disease, infectious salmon anemia (ISA), vibriosis, and white spot disease. Technological advancements have played a crucial role in enhancing our understanding of fish immunological mechanisms, leading to improved vaccine administration and the development of recombinant live attenuated, subunit, DNA, and RNA vaccines. However, challenges such as oral tolerance, vaccine degradation, and stressful environments persist, impacting vaccine efficacy. Addressing these challenges and gaining a deeper understanding of the fish immune system and host–pathogen interactions will be pivotal for future improvements, contributing to the sustainability of aquaculture and enhancing global food security.

Microbial ecology of protective isolation room: Air and Surfaces.

Healthcare-associated infections pose a significant public health burden, leading to morbidity, mortality, prolonged hospital stays, and substantial social and economic costs. Immunocompromised patients are at a heightened risk of nosocomial infections. This prospective study conducted at Mohammed VI University Hospital of Oujda aimed to assess the microbial ecology of surfaces and air in an immunosuppressed patient room compared to a double hospitalization room. Microbiological air purity tests were conducted employing both the sedimentation method and the collision method with the assistance of Microflow Alpha. The sedimentation method used Mueller Hinton with 5% human blood, facilitating the free fall of contaminated dust particles. The collection program employed was set for 10 minutes per 1 m3. For surface sampling, swabs were taken from a 25 cm2 surface. The swabs were immediately forwarded to the Microbiology Laboratory. We carried out both macroscopic and microscopic identification of colonies, followed by definitive biochemical identification using the BD phoenixTM system. Antibiotic susceptibility was assessed through agar diffusion on Muller Hinton medium coupled with the determination of the minimum inhibitory concentration. The results revealed a decreased bacterial count within the protective isolation room, in contrast to the standard hospital room. We noted the predominance of coagulase-negative Staphylococcus spp and Bacillus spp. Staphylococcus aureus and Aspergillus spp, common pathogens in healthcare-associated infections, were notably absent in the protective isolation room. The findings underline the pivotal role of hospital environments in the transmission of healthcare-associated infections. The protective isolation room demonstrated effective control of microbial contamination, with fewer and less resistant germs. The study highlighted the significance of air treatment systems in preventing the spread of opportunistic infections. Our study underscored the critical role of microbiological cleanliness in preventing nosocomial infections.

Capturing the implications of residential segregation for the dynamics of infectious disease transmission.

Occupational and residential segregation and other manifestations of social and economic inequity drive of racial and socioeconomic inequities in infection, severe disease, and death from a wide variety of infections including SARS-CoV-2, influenza, HIV, tuberculosis, and many others. Despite a deep and long-standing quantitative and qualitative literature on infectious disease inequity, mathematical models that give equally serious attention to the social and biological dynamics underlying infection inequity remain rare. In this paper, we develop a simple transmission model that accounts for the mechanistic relationship between residential segregation on inequity in infection outcomes. We conceptualize segregation as a high-level, fundamental social cause of infection inequity that impacts both who-contacts-whom (separation or preferential mixing) as well as the risk of infection upon exposure (vulnerability). We show that the basic reproduction number, ℛ 0 , and epidemic dynamics are sensitive to the interaction between these factors. Specifically, our analytical and simulation results and that separation alone is insufficient to explain segregation-associated differences in infection risks, and that increasing separation only results in the concentration of risk in segregated populations when it is accompanied by increasing vulnerability. Overall, this work shows why it is important to carefully consider the causal linkages and correlations between high-level social determinants - like segregation - and more-proximal transmission mechanisms when either crafting or evaluating public health policies. While the framework applied in this analysis is deliberately simple, it lays the groundwork for future, data-driven explorations of the mechanistic impact of residential segregation on infection inequities.

Strategic ventilation design for reducing airborne infection transmission in a two-story building: A numerical approach

This study employs Computational Fluid Dynamics (CFD) to assess the impact of various ventilation configurations on respiratory droplet dispersion in a two-story restaurant building, aiming to reduce airborne infection transmission. A total of 24 scenarios, involving three ventilation configurations and eight individuals as potential droplet sources, are studied. The configurations—Case A, Case B, and Case C—are analyzed for their effectiveness in controlling droplet dispersion and improving air exchange effectiveness (AEE). Case B, with an outlet near occupants and an inlet above the zone separation, proved most effective in minimizing the droplet transmission between floors, reducing suspended airborne droplets on each floor, and preventing surface contamination, thereby enhancing indoor air quality. Despite the positioning inlet and outlet vents near crowded areas, Case A falls short in effectively removing droplets due to the intricate airflow patterns caused by air diffusers. Case C showed larger AEE but was less effective in droplet dispersion control, indicating a crucial balance between optimizing air circulation and limiting pathogen dispersion is needed. The findings underscore the importance of strategic ventilation design in mitigating airborne infection transmission in multi-level buildings. An optimal configuration, as demonstrated by Case B, combines efficient air distribution with strategic placement of ventilation components to create barriers against interzonal droplet transmission. This study highlights the potential of targeted ventilation strategies to significantly reduce the risk of airborne infection in enclosed, populated spaces.

RSV in Young Children During and After the COVID-19 Pandemic-Implications for Delaying the Age of First Infection.

Nonpharmaceutical interventions introduced in the United Kingdom's response to the COVID-19 pandemic disrupted the transmission of other childhood infections. We report changes in seasonality, age dynamics and severity of respiratory syncytial virus infections between 2019 and 2023. These data show the potential effects of delaying respiratory syncytial virus exposure and may provide insights for the implementation of treatments preventing infection during early infancy.

Genetic diversity of porcine circoviruses 2 and 3 circulating among wild boars in the Moscow Region of Russia.

Porcine circoviruses (PCVs) are widely distributed in swine herds. PCV2, the significant swine pathogen, causes infections characterized by growth and development disorders, skin lesions, and respiratory distress. PCV3 has been circulating worldwide and can be associated with various clinical signs and disease developments. Wild boars are the main reservoir of these pathogens in wildlife and can create an alarming threat to pig farming. In Russia, three PCV2 genotypes (PCV2a, PCV2b, and PCV2d) were identified in pig farms. Additionally, PCV3 was observed in pig herds during the monitoring studies in the country. However, data considering the circulation of PCVs in herds of wild boars in Russia is scant. For this purpose, we performed PCR assays of the samples from 30 wild boars hunted in the Moscow Region of Russia in 2021-2023. The ratios of wild boars positive for PCV2, PCV3, or coinfected were 50, 10, and 13.3%, respectively. Additionally, we sequenced 15 PCV2 and four PCV3 complete genomes and conducted phylogenetic analysis, which divided PCV2 isolates into two groups: PCV2d and PCV2b. The study showed a high infection rate of PCV2 among wild boars, with PCV2d dominance. Simultaneously, PCV3 also circulates among wild boars. The obtained results can provide a basis for the development of preventive measures to support infection transmission risks between farm and wild animals.

Description of New Morphological Variation of Culex (Culex) coronator Dyar and Knab, 1906 and First Report of Culex (Carrollia) bonnei Dyar, 1921 Found in the Central Region of Peru

Mosquitoes (Diptera: Culicidae) pose a significant threat to public health worldwide, especially in tropical and subtropical regions, where they act as primary vectors in transmission of infectious agents. In Peru, 182 culicid species have been identified and several species of the genus Culex are known to transmit arboviruses. However, knowledge of mosquito diversity and distribution remains limited, with many studies focusing on specific regions only. Here, we describe a new morphological variation of Cx. (Culex) coronator Dyar and Knab, 1906, and report the presence of Culex (Carrollia) bonnei Dyar, 1921 in the central region of Peru, Huanuco. Specimens were obtained through larvae collections and identified through morphologic characterization, including dissection of male genitalia, and molecular analyses. In total, 17 mosquitoes were analyzed, and the genitalia of the male specimens allowed the identification of Cx. coronator and Cx. bonnei. Partial sequences of the CoxI gene corresponding to these two species were obtained (N = 10). Phylogenetic analysis revealed that the sequences of Cx. coronator grouped in a monophyletic clade with sequences ascribed to other species corresponding to the subgenus Carrollia, while Cx. bonnei specimens formed a monophyletic clade with homologous sequences from GenBank. This study underscores the importance of continued efforts to study the diversity and distribution of mosquitoes in Peru, including their potential role as vectors of human pathogens, to underpin effective disease control and prevention strategies, highlighting the importance of a complemented morphological and molecular analysis.

Spatial examination of social and environmental drivers of Middle East respiratory syndrome coronavirus (MERS-CoV) across Kenya.

Climate and agricultural land-use change has increased the likelihood of infectious disease emergence and transmissions, but these drivers are often examined separately as combined effects are ignored. Further, seldom are the influence of climate and agricultural land use on emerging infectious diseases examined in a spatially explicit way at regional scales. Our objective in this study was to spatially examine the climate, agriculture, and socio-demographic factors related to agro-pastoralism, and especially the combined effects of these variables that can influence the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels across northern Kenya. Our research questions focused on: (1) How MERS-CoV in dromedary camels has varied across geographic regions of northern Kenya, and (2) what climate, agriculture, and socio-demographic factors of agro-pastoralism were spatially related to the geographic variation of MERS-CoV cases in dromedary camels. To answer our questions, we analyzed the spatial distribution of historical cases based on serological evidence of MERS-CoV at the county level and applied spatial statistical analysis to examine the spatial relationships of the MERS-CoV cases between 2016 and 2018 to climate, agriculture, and socio-demographic factors of agro-pastoralism. Regional differences in MERS-CoV cases were spatially correlated with both social and environmental factors, and particularly ethno-religious camel practices, which highlight the complexity in the distribution of MERS-CoV in dromedary camels across Kenya.

Knowledge of pregnant women regarding prevention of mother-to-child transmission of HIV infection in Gert Sibande District, Mpumalanga Province, South Africa: A qualitative study.

Prevention of mother-to-child transmission (PMTCT) of HIV helps in closing the gaps for new HIV infections, thereby contributing to achieving the global targets of an AIDS-free generation. To explore knowledge regarding PMTCT among pregnant women in Gert Sibande District, Mpumalanga Province, South Africa (SA). The study was performed in two clinics in Chief Albert Luthuli Municipality, Gert Sibande District. It was a qualitative and descriptive exploratory study involving in-depth, one-on-one interviews with pregnant women regarding their PMTCT knowledge and perspectives. The study findings showed that pregnant women from the two clinics have a good knowledge of PMTCT. However, they were not aware that caesarean section can minimise mother-to-child transmission (MTCT) of HIV infection. PMTCT is important in establishing an HIV-free generation. The study revealed that women had a good understanding of MTCT; nevertheless, additional education is necessary, particularly regarding birthing procedures that minimise the risk of MTCT.

Comprehensive profiling of social mixing patterns in resource poor countries: A mixed methods research protocol.

Low-and-middle-income countries (LMICs) bear a disproportionate burden of communicable diseases. Social interaction data inform infectious disease models and disease prevention strategies. The variations in demographics and contact patterns across ages, cultures, and locations significantly impact infectious disease dynamics and pathogen transmission. LMICs lack sufficient social interaction data for infectious disease modeling. To address this gap, we will collect qualitative and quantitative data from eight study sites (encompassing both rural and urban settings) across Guatemala, India, Pakistan, and Mozambique. We will conduct focus group discussions and cognitive interviews to assess the feasibility and acceptability of our data collection tools at each site. Thematic and rapid analyses will help to identify key themes and categories through coding, guiding the design of quantitative data collection tools (enrollment survey, contact diaries, exit survey, and wearable proximity sensors) and the implementation of study procedures. We will create three age-specific contact matrices (physical, nonphysical, and both) at each study site using data from standardized contact diaries to characterize the patterns of social mixing. Regression analysis will be conducted to identify key drivers of contacts. We will comprehensively profile the frequency, duration, and intensity of infants' interactions with household members using high resolution data from the proximity sensors and calculating infants' proximity score (fraction of time spent by each household member in proximity with the infant, over the total infant contact time) for each household member. Our qualitative data yielded insights into the perceptions and acceptability of contact diaries and wearable proximity sensors for collecting social mixing data in LMICs. The quantitative data will allow a more accurate representation of human interactions that lead to the transmission of pathogens through close contact in LMICs. Our findings will provide more appropriate social mixing data for parameterizing mathematical models of LMIC populations. Our study tools could be adapted for other studies.