Transmission Of Helicobacter Pylori Research Articles

Breastfeeding Is Associated with Lower Likelihood of Helicobacter Pylori Colonization in Babies, Based on a Prospective USA Maternal-Infant Cohort.

Mother-to-child transmission of Helicobacter pylori (H. pylori) is the primary source of intrafamilial spread in early childhood in regions of high H. pylori prevalence. However, early-in-life H. pylori colonization and associated protective or risk factors have not been fully evaluated in lower prevalence regions, such as the USA. Therefore, from a well-characterized prospective US cohort, we selected women who provided fecal samples during pregnancy and had paired fecal samples from their babies up to 24months postpartum. We evaluated maternal and baby factors associated with likelihood of H. pylori colonization in the babies. Fecal antigen testing was used to determine H. pylori status. We also evaluated the association between maternal breastmilk cytokines and H. pylori colonization in breastfed babies. Among included mother-baby pairs (n = 66), H. pylori prevalence was 31.8% in mothers and 19.7% in their babies. Dominant breastfeeding (adjusted odds ratio [aOR] 0.17, 95% CI 0.03-0.98) and maternal IBD (aOR 0.05, 95% CI 0.01-0.27) were associated with significantly lower likelihood of H. pylori colonization among babies; no other clinical factors were associated with H. pylori colonization in the babies. Matrix metalloproteinase-10 (MMP-10) and tumor necrosis factor-related activation-induced cytokine expression were significantly higher in breastmilk of mothers with H. pylori positive vs negative babies. Consistent with data from high H. pylori prevalence regions, our findings suggest dominant breastfeeding may protect against early H. pylori colonization. Downregulation of pro-inflammatory cytokines such as MMP-10 may be relevant in mediating this protection among breastfed babies, but more data are needed.

Investigation of Helicobacter pylori transmission between spouses

Investigation of Helicobacter pylori transmission between spouses

Retracted: Vacuolating cytotoxin A (vacA) and cytotoxin‐associated gene A (cagA) genotypes of resistant Helicobacter pylori strains isolated from raw and pasteurized milk

Foods with animal origins play a considerable role in transmission of Helicobacter pylori. The current study was done to evaluate the genotyping patterns and antibiotic resistance properties of H. pylori strains isolated from raw and pasteurized milk. Three hundred and fifty raw and pasteurized milk samples were collected. Samples were cultured on Wilkins Chalgren media. Antibiotic resistance pattern and genotyping were done using the disk diffusion and polymerase chain reaction, respectively. Sixty‐eight out of 350 milk samples (19.42%) were positive for H. pylori. Prevalence of H. pylori in raw and pasteurized milk samples were 44.00% and 1.00%, respectively. Helicobacter pylori strains exhibited the maximum prevalence of resistance against tetracycline (94.11%), ampicillin (91.17%), amoxicillin (88.23%), and erythromycin (69.11%). VacA s1a (98.52%), m1a (88.23%), m2 (79.41%), and s2 (77.94%) and also vacA s1am1a (61.76%), s1am2 (51.94%), s2m2 (51.94%), and s2m1a (50.00%) were the most frequently detected genotypes. VacA s2/cagA+ (50.00%), s1a/cagA+ (47.05%), m1a/cagA+ (41.17%), and m2/cagA+ (33.82%) were the most frequently detected combined genotyping patterns. Boost prevalence of H. pylori in raw and pasteurized milk exhibits that they may be the natural reservoirs of bacteria. Similar vacA and cagA genotyping patterns of H. pylori strains recommends that they have similar routes of infection.Practical applicationsDue to the high consumption rate of raw and pasteurized milk samples, they should have a high microbial quality. Foods with animal origins and especially milk are considered as a probable source of Helicobacter pylori infections. The incidence of contamination of milk by H. pylori is fairly high, and nearly all of the isolates showed resistance against several types of antibiotics and harbored pathogenic vacA and cagA genotypes, so the risk of probable foodborne diseases caused by H. pylori in such products should not be neglected. Additionally, raw and pasteurized milk samples may act as a reservoir of H. pylori with ability to transfer antibiotic resistance and virulent genotypes.

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

AbstractDairy products play a considerable role in transmission of Helicobacter pylori. The current study was done to assess phenotypic characters of antimicrobial resistance and genotyping pattern of vacA, cagA, and iceA alleles among the H. pylori strains isolated from dairy products. Eight‐hundred dairy samples were collected and cultured. Antimicrobial resistance and genotyping patterns were assessed using disk diffusion and polymerase chain reaction, respectively. Thirty‐one out of 800 (3.87%) traditional dairy product samples were positive for H. pylori. Cheese (11.53%) samples had the highest incidence of H. pylori, while yoghurt (1.11%) had the lowest. Helicobacter pylori strains harbored the highest incidence of resistance against ampicillin (93.54%), amoxicillin (93.54%), tetracycline (90.32%), erythromycin (80.64%), and metronidazole (77.41%). VacA s1a (90.32%), m1a (90.32%), s2 (80.64%) and m2 (77.19%), cagA (51.61%), and iceA1 (32.25%) were the most routinely identified genotypes. S1am1a (67.74%), s2m1a (64.51%), and s1am2 (51.61%) were the most routinely identified genotyping pattern. Simultaneous presence of vacA, cagA, and iceA genotypes in antimicrobial resistant H. pylori strains indicates important public health issue regarding the consumption of dairy products. However, additional researches are required to find molecular genetic homology and other epidemiological aspects of H. pylori in dairy products.Practical applicationsDue to the high consumption rate of dairy samples, they should have a high microbial quality. Foods with animal origins and especially dairy samples are considered as a probable source of Helicobacter pylori infections. The incidence of contamination of dairy samples by H. pylori is fairly high, and nearly all of the isolates exhibited resistance against several types of antimicrobials and harbored pathogenic vacA, cagA, and iceA genotypes, so the risk of probable foodborne diseases caused by H. pylori in such products should not be neglected. Additionally, dairy samples may act as a reservoir of H. pylori with ability to transfer antimicrobial resistance and virulent genotypes.

Phenotypic analysis of antibiotic resistance and genotypic study of the vacA, cagA, iceA, oipA and babA genotypes of the Helicobacter pylori strains isolated from raw milk

BackgroundFoods with animal origins and particularly milk play a considerable role in transmission of Helicobacter pylori. The current study was performed to assess phenotypic characters of antibiotic resistance and genotyping pattern of vacA, cagA, iceA, oipA and babA2 alleles amongst the H. pylori strains isolated from raw milk.MethodsSix-hundred and thirty raw milk samples were collected and cultured on Wilkins Chalgren anaerobe media. Antibiotic resistance and genotyping patterns were studied using disk diffusion and PCR, respectively.ResultsSixty-seven out of 630 (10.63%) raw milk samples were positive for H. pylori. Ovine raw milk (17.27%) samples had the highest prevalence of H. pylori, while camel (5.00%) had the lowest. H. pylori strains harbored the highest prevalence of resistance against ampicillin (82.08%), tetracycline (76.11%), amoxicillin (74.62%), metronidazole (65.67%) and erythromycin (53.73%). Prevalence of resistance against more than 10 types of antibiotics was 17.91%. VacA s1a (83.58%), m1a (80.59%), s2 (77.61%) and m2 (68.65%), cagA (73.13%) and babA2 (44.77%) were the most commonly detected genotypes. We found that S1am1a (56.71%), s2m1a (56.71%), s1 am2 (43.28%) and s2 m2 (43.28%) were the most commonly detected genotyping pattern. Frequency of cagA-, oipA- and babA2- genotypes were 26.86%, 62.68% and 55.22%, respectively. We found that S1a/cagA+/iceA1/oipA−/babA2- (28.35%), m1a/cagA+/iceA1/oipA−/babA2- (28.35%) and s2/cagA+/iceA1/oipA−/babA2- (26.86%) were the most commonly detected combined genotyping pattern.ConclusionsSimultaneous presence of vacA, cagA, iceA, oipA and babA2 genotypes in antibiotic resistant H. pylori strains indicates important public health issue regarding the consumption of raw milk. However, additional researches are required to find molecular genetic homology and other epidemiological aspects of H. pylori in milk.

Population Knowledge, Attitude, and Practice Regarding Helicobacter pylori Transmission and Outcomes: A Literature Review.

Helicobacter pylori infection is associated with the development of chronic gastritis, peptic ulcer disease, and gastric cancer. Current clinical recommendations are that H. pylori test-and-treat should be individualized based on comorbidities and patient preferences among populations at increased risk for certain morbidities. However, knowledge, attitudes and practices regarding H. pylori among potential patient populations are largely unknown. We conducted a literature review to assess knowledge, attitudes, and practices of patients or community populations around H. pylori transmission, prevention, and associated morbidity. Nine studies met the inclusion criteria, all published between 1997 and 2014. Eight studies evaluated perception of H. pylori among at-risk populations, while one study evaluated perception among a general population. The studies suggest inconsistencies between the perceptions of these populations and the established understanding of knowledge, attitude, and preventive practices for H. pylori among even at-risk populations. To adequately respond to current test-and-treat recommendations for treatment of H. pylori, general population education must be implemented, especially among at-risk populations. Further work is needed within at-risk populations in the United States to determine prevalence of H. pylori and their current knowledge if adequate prevention strategies are to be designed.

Role of food in environmental transmission of Helicobacter pylori.

Helicobacter pylori (H.pylori) is a gram-negative bacterium that has infected more than half of the world's population. This pathogen colonizes the human gastric mucosa and is usually acquired during childhood. It is an important cause of peptic ulcers, chronic gastritis and stomach cancer. Among the risk factors for acquisition of H. pylori infection, poor socioeconomic status, poor sanitization and hygiene practices, and contaminated food and water, are the most significant ones. The main route of H. pylori transmission is still unknown. Studies show that H.pylori bacteria can spread directly from one person to the other, or indirectly from an infected person to the environment. Person to person transmission is divided into fecal-oral, gastric-oral, oral-oral, sexual routes. Presently, interpersonal pathways are more acceptable than environmental exposure routes. Literatures indicate the presence and survival of H. pylori in food samples, such as milk, vegetables and meat, and suggest these foods may play an important role in the environmental transmission of this pathogen. In addition, other studies report the presence of H. pylori in the gastric tissue of some animals (e.g. sheep and cow) and therefore, it is likely they participate in the food chain transmission as reservoirs besides human. Although there are findings which indicate the probable role of food products in the environmental transmission of H. pylori, there is still not enough direct evidence to confirm this and more studies are needed. However, attention to food contamination sources (unhygienic water) and controlling them may prevent transmission of pathogens associated with health.

Survival of Helicobacter pylori in the wastewater treatment process and the receiving river in Michigan, USA.

Contaminated water may play a key role in the transmission of Helicobacter pylori, resulting in gastrointestinal diseases in humans. The wastewater treatment process is an important barrier to control the transmission of H. pylori. However, the presence and viability of H. pylori in the treatment process is not well known. In this paper, the real colony morphology of H. pylori was confirmed by two types of culture media. The survival of H. pylori through the tertiary wastewater treatment process, especially UV disinfection, and in the receiving Huron River in Ann Arbor, Michigan, was investigated by plates cultivation, regular polymerase chain reaction (PCR) assays and quantitative real-time PCR from DNA. The results demonstrated that H. pylori was not only present, but also viable in all processed wastewater samples in the Ann Arbor wastewater treatment plant (WWTP). H. pylori can be found in a higher concentration in the receiving Huron River. There are many kinds of antibiotic- and UV-resistant bacteria, including H. pylori, in the final effluent of Ann Arbor WWTP.

Н.pylori-associated gastroduodenal pathology in early age infants: own clinical observation

The literature data concerning the problem of Helicobacter pylori infection in young children during last 10 years are presented in the article. Also results of own clinical observation of 2 children with Helicobacter-associated gastroduodenopathy were shown. Possible prophylactic measures of perinatal transmission of Helicobacter pylori and postnatal infection in infants accounting the world experience were described.

A study on the transmission of <i>helicobacter pylori</i> from food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples

Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in 1000 people across India. The Ratio of Male: female was 700: 300 with age group ranging from 20 - 39. Three gastric biopsies were collected for identification of Helicobacter pylori. PCR (Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis showed the prevalence of H. pylori as 70.8% in individuals frequently consuming food prepared under less hygienic condition (street vendor’s food) whereas 60% (less prevalence) in individuals consuming food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s food is not directly involved in the transmission of this bacterium. The unhygienic preparation of food and poor personal hygiene play a vital role in the transmission of these bacteria.

A Cross-Sectional Study of Helicobacter Infection Among Laboratory Animals and Animal Research Workers

A Cross-Sectional Study of Helicobacter Infection Among Laboratory Animals and Animal Research Workers

19 Does Drinking and Environmental Water Play a Role As a Reservoir in Helicobacter pylori Transmission? Evidence From the South Region of Poland

19 Does Drinking and Environmental Water Play a Role As a Reservoir in Helicobacter pylori Transmission? Evidence From the South Region of Poland

Helicobacter Pylori Persistence in Children: Distinguishing Inadequate Treatment, Resistant Organisms, and Reinfection

Helicobacter pylori is a worldwide infection that causes chronic gastritis, duodenal ulcers, and malignancy. Transmission of Helicobacter pylori within a family appears to be the predominant mode of contamination. Recurrence of the infection is frequently seen following treatment. Lack of eradication due to either inadequate treatment or resistant bacteria vs. reinfection have been explanations for detection of H. pylori following treatment. In this article we will discuss the concepts of inadequate treatment vs. resistant infection and reinfection as causes of persistent H. pylori infection.

Did transmission of Helicobacter pylori from humans cause a disease outbreak in a colony of Stripe-faced Dunnarts (Sminthopsis macroura)?

Since the discovery that Helicobacter pylori causes a range of pathologies in the stomachs of infected humans, it has become apparent that Helicobacters are found in a diverse range of animal species where they are frequently associated with disease. In 2003 and 2004, there were two outbreaks of increased mortality associated with gastric bleeding and weight-loss in a captive colony of the Australian marsupial, the Stripe-faced Dunnart (Sminthopsis macroura). The presence of gastric pathology led to an investigation of potential Helicobacter pathogenesis in these animals. Histological examination revealed the presence of gastritis, and PCR analysis confirmed the presence of Helicobacter infection in the stomachs of these marsupials. Surprisingly, sequencing of 16S rRNA from these bacteria identified the species as H. pylori and PCR confirmed the strain to be positive for the important pathogenesis factor, cagA. We therefore describe, for the first time, an apparent reverse zoonotic infection of Stripe-faced Dunnarts with H. pylori. Already prone to pathological effects of stress (as experienced during breeding season), concomitant H. pylori infection appears to be a possible essential but not sufficient co-factor in prototypic gastric bleeding and weight loss in these marsupials. The Stripe-faced Dunnart could represent a new model for investigating Helicobacter-driven gastric pathology. Infections from their human handlers, specifically of H. pylori, may be a potential risk to captive colonies of marsupials.

Effect of Dental Plaque Control on Infection of Helicobacter pylori in Gastric Mucosa

Data on the role of dental plaque in the transmission of Helicobacter pylori have varied. Furthermore, there has been few reports on the relationship between dental plaque control and H. pylori infection of gastric mucosa. The purpose of this study was to elucidate this potential relationship. The (13)C urea breath test was conducted on 56 subjects who received dental plaque control and 51 subjects who did not. The prevalence of H. pylori in the gastric mucosa was 19.64% in patients who received dental plaque control, which was significantly lower than in those without dental plaque control (84.31%). Long-term professional dental plaque control was associated with less gastric reinfection by H. pylori, suggesting that dental plaque control may help to prevent H. pylori-induced gastric disease or reinfection.

Transmission of Helicobacter pylori and the role of water and biofilms

Documented evidence relating to the survival of Helicobacter pylori outside the gastric niche is extremely limited. To date the primary transmission routes of H. pylori have yet to be confirmed and when this is achieved preventive infection control measures can be implemented to reduce and ultimately prevent human infection from this pathogen. There is mounting evidence which suggests that the prevalence of H. pylori infection has a strong correlation with access to clean water, suggesting a transmission route to the host. However, there are no established culture methods for the detection of viable H. pylori in the environment, in particular drinking water supplies, preventing the development of true epidemiological and risk assessments. The aim of this review is to highlight the available data to date that suggests drinking water and possible survival in biofilms as a probable transmission mode for H. pylori.

Horizontal versus Familial Transmission of Helicobacter pylori

Transmission of Helicobacter pylori is thought to occur mainly during childhood, and predominantly within families. However, due to the difficulty of obtaining H. pylori isolates from large population samples and to the extensive genetic diversity between isolates, the transmission and spread of H. pylori remain poorly understood. We studied the genetic relationships of H. pylori isolated from 52 individuals of two large families living in a rural community in South Africa and from 43 individuals of 11 families living in urban settings in the United Kingdom, the United States, Korea, and Colombia. A 3,406 bp multilocus sequence haplotype was determined for a total of 142 H. pylori isolates. Isolates were assigned to biogeographic populations, and recent transmission was measured as the occurrence of non-unique isolates, i.e., isolates whose sequences were identical to those of other isolates. Members of urban families were almost always infected with isolates from the biogeographic population that is common in their location. Non-unique isolates were frequent in urban families, consistent with familial transmission between parents and children or between siblings. In contrast, the diversity of H. pylori in the South African families was much more extensive, and four distinct biogeographic populations circulated in this area. Non-unique isolates were less frequent in South African families, and there was no significant correlation between kinship and similarity of H. pylori sequences. However, individuals who lived in the same household did have an increased probability of carrying the same non-unique isolates of H. pylori, independent of kinship. We conclude that patterns of spread of H. pylori under conditions of high prevalence, such as the rural South African families, differ from those in developed countries. Horizontal transmission occurs frequently between persons who do not belong to a core family, blurring the pattern of familial transmission that is typical of developed countries. Predominantly familial transmission in urban societies is likely a result of modern living conditions with good sanitation and where physical contact between persons outside the core family is limited and regulated by societal rules. The patterns observed in rural South African families may be representative of large parts of the developing world.

Tonsillectomy Does not Decrease the Risk of Helicobacter pylori Transmission

Tonsillectomy Does not Decrease the Risk of Helicobacter pylori Transmission

The transmission of Helicobacter pylori: The effects of analysis method and study population on inference

Although much is known about the virulence of Helicobacter pylori, the transmission pathways for this bacterium are still unresolved. Transmission has been addressed through: (1) prevalence within families; (2) detection in fecal/oral environments; (3) detection in the abiotic/biotic environment; and (4) direct inference from strain similarity. Here, we review the molecular and biochemical methods used and discuss the relative merits of each. Furthermore, as there are differences between developing and developed nations, we discuss the results obtained from transmission studies in light of the study population. We conclude that H. pylori is probably transmitted person-to-person, facilitated by fecal-oral transmission during episodes of diarrhea or gastro-oral contact during periods of vomiting. The persistence of H. pylori in abiotic and biotic environments remains unproven but possible reactivation from viable, non-culturable coccoid forms should be further investigated. Finally, we speculate on the effect of host-pathogen interactions in confounding the inference of transmission.

P1938 Use of nested PCR for evaluation of Helicobacter pylori transmission by endoscopies

P1938 Use of nested PCR for evaluation of Helicobacter pylori transmission by endoscopies