Cadmium Translocation Research Articles

Effect of mixing oak leaf biomass with soil on cadmium toxicity and translocation in tomato genotypes

The environmental non-element cadmium (Cd) is toxic to all forms of life, and it also has a negative impact on plant development and growth. In order to ascertain the effects of cadmium on tomato growth and the function of oak leaf biomass in the reduction of toxicity and translocation of cadmium in different parts of tomato genotypes, two tolerant and two sensitive tomato genotypes were exposed to cadmium stress through the availability or unavailability of oak leaf biomass. The experiment involved two factors. The first factor was the various treatment levels, including soil without Cd treatment and sodium hydroxide (NaOH) oak leaf biomass pretreatment (COC-control), soil with Cd treatment and without NaOH oak leaf biomass pretreatment (CdC), and soil with Cd treatment and NaOH oak leaf biomass pretreatment (CdOBC). The second element consists of four tomato genotypes. Comparing to control conditions, all tomato genotypes spotted significant reductions in all morphological traits under Cd stress in the presence or absence of NaOH oak leaf pretreatment. Related to CdC conditions, root length, shoot length, root fresh weight per plant, shoot fresh weight per plant, root dry weight per plant, shoot dry weight per plant, and total fruit weight per plant were significantly improved by 4.25%, 9.75%, 23.24%, 10.10%, 28.10%, 9.08%, and 4.61%, respectively, under the availability of pretreatment of oak leaf biomass. The tolerant genotypes (Karazi and Sirin) exhibited the greatest increase in all traits evaluated, with the exception of root length, under the CdOBC condition compared to the CdC statement. Significant increases in leaf biochemical parameters were seen with the availability or absence of NaOH pretreatment of oak leaf biomass in the soil. The maximum values of proline content, soluble sugar content, antioxidant activity, and guaiacol peroxidase were stated in the presence of oak biomass under Cd conditions (CdOBC), with mean values of 1772.46 μg g −1, 687.18 μg g −1, 1025.74 μg g −1-, and 0.43 units min −1 g −1, respectively. The in vitro-tolerant genotypes exhibited the maximum values of all biochemical parameters. The concentration of cadmium in the studied tomato genotypes revealed that cadmium accumulated more in the roots than other parts. According to these outcomes, NaOH pretreatment of oak leaf biomass can be employed to diminish the hazard of cadmium absorption by edible parts.

Metallochaperone protein OsHIPP17 regulates the absorption and translocation of cadmium in rice (Oryza sativa L.)

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in regulating heavy metal responding activities in plants. Yet only a handful of studies have characterized the functions of HIPPs. In this study, a novel HIPP member OsHIPP17 was functionally characterized, which was involved in the tolerance of yeast and plants to cadmium (Cd). The Cd accumulation in yeast cells was increased due to the overexpression of OsHIPP17. Nevertheless, the overexpression of OsHIPP17 in Arabidopsis thaliana resulted in compromised growth under Cd stress. Meanwhile, the mutation of OsHIPP17 resulted in 38.9–40.9 % increase of Cd concentration in rice roots as well as 14.3–20.0 % decrease of Cd translocation factor. Further investigation of the genes responsible for Cd absorption and transporter indicated that the expression levels of these genes were also perturbed. In addition, two OsHIPP17-interacting proteins, OsHIPP24 and OsLOL3 were identified in a yeast two hybrid assay. Further analysis of their functions revealed that OsHIPP24 or OsLOL3 may be involved in the regulation of Cd tolerance by OsHIPP17 in rice. All above results implied that OsHIPP17 may affect Cd resistance by regulating the absorption and translocation of Cd in rice.

In vitro characterization of cadmium transport across the gastro-intestinal membrane of the fathead minnow (Pimephales promelas) in the presence and absence of microplastics

There is concern that microplastics can act as a vector for cadmium through adsorption and desorption of free-ionic cadmium. Little is known about the uptake of cadmium following ingestion of cadmium-microplastic complexes. This study used an in vitro gut sac technique to investigate the translocation of cadmium across the gut barrier of fathead minnows following the simulated ingestion of cadmium, microplastics, or their complexed mixture. Microplastics did not cross the gut membrane, nor did microplastics alter the rate of cadmium translocation, which was estimated to be 1.2 ± 0.04 ng Cd / hour. Less cadmium translocated when cadmium-microplastic complexes were injected than the equivalent dose of only cadmium, indicating that the presence of microplastics was protective of dietary cadmium uptake. This work highlights the importance of considering dietary uptake and the role of microplastics acting as a vector for cadmium in aquatic environments and stresses the need to understand how environmental (digestive or ambient) characteristics govern cadmium-microplastic interactions.

Uptake and translocation of lead and cadmium in wild-found plant species from Bekasi and Karawang, West Java, for phytoremediation

Environmental degradation due to lead (Pb) and cadmium (Cd) pollution has been increasing. One of the alternative cost-effective green technologies to clean up heavy metal contamination is phytoremediation. This research aims to determine the potential of wild-found plants that could be used as Pb and Cd bioaccumulators. Plant species in this study were collected from heavy metal-contaminated soil in Bekasi and Karawang, West Java. Five species, namely Saccharum spontaneum, Acorus calamus, Ipomoea fistulosa, Ludwigia hyssopifolia, and Eichhornia crassipes, were studied for Pb accumulation capacity. Furthermore, five plant species, namely Limnocharis flava, Colocasia esculenta, Ipomoea fistulosa, Commelina benghalensis, and Eichhornia crassipes, were studied for their Cd accumulation capacity. The experiment was done in a greenhouse for eight weeks. Pb and Cd concentration were analyzed using atomic absorption spectrometry (AAS) to determine the uptake and translocation of Pb or Cd. I. fistulosa accumulated the highest amount of Pb, with a growth rate of up to 27.07 g week−1, a bioconcentration factor (BCF) of 1.46, and a translocation factor (TF) of 0.87 upon 300 mg kg−1 Pb treatment. C. esculenta showed considerable Cd bioaccumulation, as indicated by a BCF of 0.95 and a TF of 0.65, accompanied by sustained relative biomass increase (124.9%) and the highest growth rate (36.96 g week−1) among tested plant species upon 75 mg kg−1 Cd treatment. With their capacities for bioaccumulating Pb and Cd, respectively, I. fistulosa and C. esculenta were identified as potential accumulator species for phytoremediation in heavy metal-contaminated sites such as former mining lands, landfills, and highly polluted agricultural lands.

Hydrogen peroxide reduces root cadmium uptake but facilitates root-to-shoot cadmium translocation in rice through modulating cadmium transporters

Cadmium (Cd) contamination in agricultural soils has become a serious worldwide environmental problem threatening crop production and human health. Hydrogen peroxide (H2O2) is a critical second messenger in plant response to Cd exposure. However, its role in Cd accumulation in various organs of plants and the mechanistic basis of this regulation remains to be elucidated. In this study, we used electrophysiological and molecular approaches to understand how H2O2 regulates Cd uptake and translocation in rice plants. Our results showed that the pretreatment of H2O2 significantly reduced Cd uptake by rice roots, which was associated with the downregulation of OsNRAMP1 and OsNRAMP5. On the other hand, H2O2 promoted the root-to-shoot translocation of Cd, which might be attributed to the upregulation of OsHMA2 critical for Cd2+ phloem loading and the downregulation of OsHMA3 involved in the vacuolar compartmentalization of Cd2+, leading to the increased Cd accumulation in rice shoots. Furthermore, such regulatory effects of H2O2 on Cd uptake and translocation were notably amplified by the elevated level of exogenous calcium (Ca). Collectively, our results suggest that H2O2 can inhibit Cd uptake but increase root to shoot translocation through modulating the transcriptional levels of genes encoding Cd transporters, furthermore, application of Ca can amplify this effect. These findings will broaden our understanding of the regulatory mechanisms of Cd transport in rice plants and provide theoretical foundation for breeding rice for low Cd accumulation.

Selenite inhibited cadmium translocation and stimulated root growth of Brassica rapa L.: Regulation of element uptake, polysaccharide synthesis and crosslink, and cell wall enzymes

Previously studies have demonstrated that selenium (Se) can reduce cadmium (Cd) concentration in plants. However, its underlying mechanisms on relationships among Cd uptake/translocation, plant root growth, essential elemental uptake, cell wall components (CWCs), and cell wall enzymes were not fully elucidated. In this study, a hydroponic system was set up to explore the above relationships in a pakchoi variety (Brassica rapa L., LvYou 102) exposed to solo– or co– exposure of Cd (0.5 mg L–1) and Se (0.5 mg L–1) using a combined analysis of physiology and transcriptome. The results showed that Se reduced shoot Cd concentration and its translocation from roots to shoots. Se significantly stimulated root growth and root concentrations of potassium and magnesium, but reduced shoot concentrations of all tested essential elements as compared to the Cd treatment alone. Se significantly enhanced galacturonic acid (GalA) and total sugar concentrations in pectins but reduced that in hemicellulose I (HCI); while Se significantly increased GalA concentration but reduced total sugar concentration in hemicellulose II (HCII) when compared to the Cd treatment alone. Se enhanced activities of pectinesterase (PME), β–galactosidase (β–Gal), and xyloglucan endoglycosidase/hydrolase (XTH), but did not alter that of polygalacturonase (PG) and cellulase (CE) when compared to the Cd treatment alone. Under Cd exposure, Se in most cases down–regulated genes correlated with elemental uptake, degradation of pectins and hemicelluloses, and synthesis of cellulose and lignins, except for some up–regulated genes including XTH27, XTH22 and BraA10g 021100.3 C.

Effect of boron supply on the uptake and translocation of cadmium in Capsicum annuum

Large areas of soil in southern China are contaminated with cadmium (Cd) and are deficient in boron (B). Previously, we suggested that B supplementation could reduce Cd accumulation in hot peppers (Capsicum annuum L.); however, the physiological mechanisms underlying this reduction remain unclear. In this study, the uptake and translocation of Cd in hot pepper plants were investigated using hydroponic experiments with different B and Cd treatments. A pot experiment was performed to verify whether B decreased the Cd concentration in hot peppers by minimizing the Cd translocation rate. The results of the dose- and time-dependent experiments showed that B supplementation reduced root Cd uptake and root-to-shoot Cd translocation. Additionally, B supplementation increased the root length, diameter, volume, surface area, and number of root forks and tips, as well as improving the relative absorbance of carboxyl groups under Cd exposure, leading to enhanced Cd fixation in the cell walls of the roots. As a result, the fruit Cd concentration decreased because B inhibited Cd translocation from the roots. Overall, the results demonstrate that B supplementation can reduce Cd accumulation in hot peppers by promoting normal root growth and development and by limiting the uptake and translocation of Cd.

Transcriptome analysis reveals different mechanisms of selenite and selenate regulation of cadmium translocation in Brassica rapa

Selenium (Se) inhibits cadmium (Cd) root-to-shoot translocation and accumulation in the shoots of pak choi; however, the mechanism by which Se regulates Cd retention in roots is still poorly understood. A time-dependent hydroponic experiment was conducted to compare the effects of selenite and selenate on Cd translocation and retention in the roots. The underlying mechanisms were investigated regarding Se biotransformation and metal transportation in roots using HPLC and transcriptome analyses. Selenite showed reducing effects on Cd translocation and accumulation in shoots earlier than selenate. Selenite is mainly biotransformed into selenomethionine (80% of total Se in roots) at 72 h, while SeO42− was the dominant species in the selenate treatments (68% in shoots). Selenite up-regulated genes involved in the biosynthesis of lignin, suberin, and phytochelatins and those involved in stress signaling, thereby helping to retain Cd in the roots, whereas essentially, selenate had opposite effects and impaired the symplastic and apoplastic retention of Cd. These results suggest that cell-wall reinforcement and Cd retention in roots may be the key processes by which Se regulates Cd accumulation, and faster biotransformation into organic seleno-compounds could lead to earlier effects.

Suppression of highly efficient cadmium translocation by exogenous gibberellin provides insights into the molecular regulation mechanism of hyperaccumulation in Sedum plumbizincicola

Sedum plumbizincicola is a hyperaccumulating species with highly efficient uptake, translocation and distribution system for cadmium (Cd), a non-essential and toxic heavy metal. To understand the molecular regulation mechanisms of Cd transport and accumulation in this hyperaccumulator is still a challenge. In this study, we observed that exogenous gibberellin A3 (GA3) significantly reduced Cd loading efficiency from root to shoot and uploading efficiency from stem to leaves in S. plumbizincicola under Cd treatments, which provided us a remarkable opportunity to study the signaling mechanisms for regulation of highly efficient Cd transport and translocation in S. plumbizincicola. Here, we constructed a de novo full-length transcriptome database with the integration of full-length and next-generation transcriptome sequences for Cd-treated S. plumbizincicola with or without exogenous GA3 application. From this database, we identified 1461 differentially expressed genes (DEGs), which were related to solute transport, cell wall metabolism and signal transduction, and most of them were down-regulated by the exogenous application of gibberellin. In particularly, a substantial number of the downregulated genes belong to calmodulin-like (CML) proteins, calcium-dependent protein kinase, calcium-permeable channel, mitochondrial calcium uniporter (MCU) and Na+/Ca2+ exchanger-like proteins (NCL/EF-CAX). These results indicated that calcium homeostasis and signal transduction may play an important role in the regulation of highly efficient translocation of cadmium in hyperaccumulator S. pumbizincicola.

Physiological and transcriptomic characterization of cadmium toxicity in Moso bamboo (Phyllostachys edulis), a non-timber forest species.

Cadmium pollution in Moso bamboo forests poses a potential threat to the sustainable development of the bamboo industry. However, the effects of cadmium toxicity on Moso growth and its mechanisms of adaptation to cadmium stress are poorly understood. In this study, the physiological and transcriptional response of Moso to cadmium stress was investigated in detail using Moso seedlings in a hydroponic system. Cadmium toxicity severely inhibited the growth of roots but had little effect on biomass accumulation in the aerial parts. Cadmium accumulation in roots and aerial parts increased as external cadmium increased, with cadmium mainly localized in the epidermis and pericycle cells in the roots. The uptake and root-to-shoot translocation of cadmium was stimulated, but the photosynthetic process was suppressed under cadmium stress. A total of 3469 differentially expressed genes were identified from the transcriptome profile and those involved in cadmium uptake, transportation and detoxification were analyzed as candidates for having roles in adaptation to cadmium stress. The results suggested that Moso is highly efficient in cadmium uptake, xylem loading and translocation, as well as having a high capacity for cadmium accumulation. This work also provided basic information on physiological and transcriptional responses of Moso to cadmium toxicity.

Role of ZnCl2 in the Uptake and Translocation of Cd to Different Parts of Wheat Plant and Risk Assessments for Cow and Human

ABSTRACT In this study, we assessed the effect of zinc (Zn) when present in form of zinc chloride (ZnCl2) on translocation and accumulation of cadmium (Cd) in different parts of wheat plants (Triticum aestivum). Along with that, health risks to organisms of higher trophic levels (cows and humans) that consume these polluted wheat plants were also calculated. For the experiments, the soil was spiked with CdCl2 at rate of 3, 6, 12, 24, and 48 mg Cd kg−1 soil and ZnCl2 at a rate of 50, 100, 200, and 300 mg Zn kg−1 soil. In the combination treatment, all Cd concentrations used in single exposure were mixed in the soil that spiked with 200 mg kg−1 of Zn. According to the data, concentration of Zn and Cd in all parts of wheat plants increase with the increase of their concentration in the soil. However, soil application of Zn reduced Cd concentration in all parts of wheat plants (approximately two-fold in root and shoot, and more than 13-fold in the grains). The highest bioaccumulation of both metals was recorded in the roots and least in grains and leaves. The translocation factor also showed that roots store most of the metals and did not translocate them to the grains. The data showed that Zn application in the soil did not decrease ecological risk factor. However, daily intake metal (DIM) and health risk index (HRI) of cows showed that the Cd intake was higher than the permissible limit, but this quantity was not a health risk for cows. In humans, DIM values were higher than the permissible limit and caused severe health risk. However, a two-fold decrease in health risk of human due to Cd was recorded in the presence of Zn. This study revealed that ZnCl2 can be used to suppress the Cd uptake in wheat even at high pollution levels. It may decrease the risk of toxicity to higher tropic levels that consume different parts of wheat plants. In future further investigation about the role of ZnCl2 in the mitigation of other metals phytotoxicity is required to use it in agroecosystem.

Methionine application at low phosphate level promotes root-to-shoot cadmium translocation by mobilizing weakly bound apoplastic cadmium in valerian (Valeriana officinalis L.)

Controlling the uptake and translocation of heavy metals in medicinal plants is crucial due to its strong impact on the safety and quality of derived medicinal plant products. This study investigated the effects of phosphate and methionine on the distribution and chemical forms of Cd in valerian (Valeriana officinalis L.) roots and shoots. Seedlings were grown for two weeks in a Cd-free nutrient solution containing either 400 or 1200 μM phosphate and then exposed for 10 days to 10 μM Cd either in combination with 400 μM or no methionine in the nutrient solution. Root Cd was partitioned into a symplastic and an apoplastic Cd fraction using a desorption procedure with EDTA. A sequential extraction method was applied to determine chemical forms in which Cd was retained in both roots and shoots. “Pectate- and protein-bound Cd” and HAc-soluble “Cd phosphates” were the dominant Cd forms in sequential extractions of both roots and shoots. Phosphate increased the HAc-soluble “Cd phosphate fraction” in the roots, but not in the shoots. Methionine did not affect this fraction, but strongly increased ethanol- and water-soluble Cd in roots and shoots. While it also increased the “pectate- and protein-bound Cd fraction” in the shoots, it decreased this fraction and the share of apoplastic Cd in the roots. The phosphate treatment reduced Zn accumulation in the roots but had no effect on Zn accumulation in the shoots, whereas Met increased shoot Zn. The results indicate that methionine induced root-to-shoot Cd translocation primarily by mobilizing weakly bound Cd to root cell walls at the low phosphate level. This could help limit Cd accumulation in valerian roots grown for medicinal use.

Auxin alleviates cadmium toxicity by increasing vacuolar compartmentalization and decreasing long-distance translocation of cadmium in Poa pratensis

Kentucky bluegrass (Poa pratensis L.) hyperaccumulates cadmium (Cd) and exhibits a hypertolerance. Thus, it has potential for the phytoremediation of Cd-containing soil. Auxin signaling is involved in the response to Cd stress. However, the mechanisms of auxin-mediated detoxification and Cd translocation in plants remain unclear. This study aimed to investigate the effects of exogenous application of indole-3-acetic acid (IAA) on the Cd translocation, subcellular Cd distribution, chemical forms of Cd, and transcriptional regulation of Kentucky bluegrass. The results showed that the exogenous application of IAA increased the amount of organelle-bound Cd and vacuole-compartmentalized Cd in root cells, reduced the Cd concentration in the leaf tissues (epidermis, mesophyll, and vascular bundle) and root tissues (rhizodermis and cortex) but increased in the stele, and alleviate Cd-induced leaf chlorosis and growth inhibition. The expression of genes associated with Cd transporters (ABCs, ZIPs, NASs, OPTs, and YSLs), phosphatases, oxalate decarboxylases and lignin biosynthesis were significantly regulated by exogenous IAA under Cd stress. A positive regulation of phosphatases and oxalate decarboxylases genes related to an increase in phosphate- and oxalate-bound Cd, as well as a decrease in pectate- and protein-bound Cd and inorganic Cd, thereby contributing to a decrease in Cd phytotoxicity. The significant regulation of Cd transporters associated with decreasing the long-distance translocation of Cd, and the activation of lignin biosynthesis may contribute to the development of root endodermal barriers and increase the deposition of undissolved Cd phosphates and oxalate-bound Cd in the stele. These results revealed the important role of auxin in Cd detoxification and translocation in Kentucky bluegrass and they provide a theoretical basis for the phytoremediation of Cd-containing soil.

From soil to cacao bean: Unravelling the pathways of cadmium translocation in a high Cd accumulating cultivar of Theobroma cacao L.

The research on strategies to reduce cadmium (Cd) accumulation in cacao beans is currently limited by a lack of understanding of the Cd transfer pathways within the cacao tree. Here, we elucidated the transfer of Cd from soil to the nib (seed) in a high Cd accumulating cacao cultivar. Here, we elucidated the transfer of Cd from soil to the nib (seed) in a high Cd accumulating cacao cultivar through Cd stable isotope fractionation, speciation (X-Ray Absorption Spectroscopy), and localization (Laser Ablation Inductively Coupled Plasma Mass Spectrometry). The plant Cd concentrations were 10-28 higher than the topsoil Cd concentrations and increased as placenta< nib< testa< pod husk< root< leaf< branch. The retention of Cd in the roots was low. Light Cd isotopes were retained in the roots whilst heavier Cd isotopes were transported to the shoots (Δ 114/110 Cd shoot-root = 0.27 ± 0.02 ‰ (weighted average ± standard deviation)). Leaf Cd isotopes were heavier than Cd in the branches (Δ 114/110 Cd IF3 leaves-branch = 0.18 ± 0.01 ‰), confirming typical trends observed in annual crops. Nibs and branches were statistically not distinguishable (Δ 114/110 Cd nib-branch = -0.08‰ ± 0.06 ‰), contrary to the leaves and nibs (Δ 114/110 Cd nib-IF3 leaves = -0.25‰ ± 0.05 ‰). These isotope fractionation patterns alluded to a more direct transfer from branches to nibs rather than from leaves to nibs. The largest fraction (57%) of total plant Cd was present in the branches where it was primarily bound to carboxyl-ligands (60-100%) and mainly localized in the phloem rays and phelloderm of the bark. Cadmium in the nibs was mainly bound to oxygen ligands (60-90%), with phytate as the most plausible ligand. The weight of evidence suggested that Cd was transferred like other nutrients from root to shoot and accumulated in the phloem rays and phelloderm of the branches to reduce the transfer to foliage. Finally, the data indicated that the main contribution of nib Cd was from the phloem tissues of the branch rather than from leaf remobilization. This study extended the limited knowledge on Cd accumulation in perennial, woody crops and revealed that the Cd pathways in cacao are markedly different than in annual crops.

Integration of Transcriptome and Metabolome Analyses Reveals the Mechanistic Basis for Cadmium Accumulation in Maize.

Cadmium (Cd) pollution in soil has become a major environmental issue worldwide. However, the underlying molecular mechanism of low grain-Cd accumulation (GCA) in maize is still largely unknown. Herein, we report the mechanistic basis for low GCA in maize by a multiomics approach. The low GCA genotype L63 showed normal vacuolar formation and a lower capacity of xylem loading of Cd than the high-accumulator L42 under Cd stress. Transcriptomic sequencing identified 84 low-GCA-associated genes which are mainly involved in the S-adenosylmethionine (SAM) cycle, metal transport, and vacuolar sequestration. A metabolome analysis revealed that L63 plants had a more active SAM cycle and a greater capacity for terpenoid synthesis and phenylalanine metabolism than L42. Combining the analysis of transcriptome and metabolome characterized several genes as key genes involved in the determination of Cd accumulation. Our study identifies a mechanistic basis for low Cd accumulation in maize grains and provides candidate genes for genetic improvement of crops.

WGCNA Analysis Revealed the Hub Genes Related to Soil Cadmium Stress in Maize Kernel (Zea mays L.).

Soil contamination by heavy metals has become a prevalent topic due to their widespread release from industry, agriculture, and other human activities. Great progress has been made in elucidating the uptake and translocation of cadmium (Cd) accumulation in rice. However, there is still little known about corresponding progress in maize. In the current study, we performed a comparative RNA-Seq-based approach to identify differentially expressed genes (DEGs) of maize immature kernel related to Cd stress. In total, 55, 92, 22, and 542 DEGs responsive to high cadmium concentration soil were identified between XNY22-CHS-8 vs. XNY22-YA-8, XNY22-CHS-24 vs. XNY22-YA-24, XNY27-CHS-8 vs. XNY27-YA-8, and XNY27-CHS-24 vs. XNY27-YA-24, respectively. The weighted gene co-expression network analysis (WGCNA) categorized the 9599 Cd stress-responsive hub genes into 37 different gene network modules. Combining the hub genes and DEGs, we obtained 71 candidate genes. Gene Ontology (GO) enrichment analysis of genes in the greenyellow module in XNY27-YA-24 and connectivity genes of these 71 candidate hub genes showed that the responses to metal ion, inorganic substance, abiotic stimulus, hydrogen peroxide, oxidative stress, stimulus, and other processes were enrichment. Moreover, five candidate genes that were responsive to Cd stress in maize kernel were detected. These results provided the putative key genes and pathways to response to Cd stress in maize kernel, and a useful dataset for unraveling the underlying mechanism of Cd accumulation in maize kernel.

S-Methylmethionine Effectively Alleviates Stress in Szarvasi-1 Energy Grass by Reducing Root-to-Shoot Cadmium Translocation.

S-methylmethionine (SMM) is a universal metabolite of higher plants derived from L-methionine that has an approved priming effect under different types of abiotic and biotic stresses. Szarvasi-1 energy grass (Elymus elongatus subsp. ponticus cv. Szarvasi-1) is a biomass plant increasingly applied in phytoremediation to stabilize or extract heavy metals. In this study, Szarvasi-1 was grown in a nutrient solution. As a priming agent, SMM was applied in 0.02, 0.05 and 0.1 mM concentrations prior to 0.01 mM Cd addition. The growth and physiological parameters, as well as the accumulation pattern of Cd and essential mineral nutrients, were investigated. Cd exposure decreased the root and shoot growth, chlorophyll concentration, stomatal conductance, photosystem II function and increased the carotenoid content. Except for stomatal conductance, SMM priming had a positive effect on these parameters compared to Cd treatment without priming. In addition, it decreased the translocation and accumulation of Cd. Cd treatment decreased K, Mg, Mn, Zn and P in the roots, and K, S, Cu and Zn in the shoots compared to the untreated control. SMM priming changed the pattern of nutrient uptake, of which Fe showed characteristic accumulation in the roots in response to increasing SMM concentrations. We have concluded that SMM priming exerts a positive effect on Cd-stressed Szarvasi-1 plants, which retained their physiological performance and growth. This ameliorative effect is suggested to be based on, at least partly, the lower root-to-shoot Cd translocation by the upregulated Fe uptake and transport.

Enhancement of Cadmium Phytoremediation Potential of Helainthus annus L. with Application of EDTA and IAA.

The aim of the current study was to assess the cadmium (Cd) phytoremediation potential of Halianthus annus L. that was exposed to 50, 100, and 150 mg/kg of cadmium for 15, 30, and 60 days with application of EDTA (Ethylenediaminetetraacetic acid) in the soil and IAA (indole acetic acid) as a foliar spray. The results indicated that the concentration, duration of exposure, and amount of Cd affect the phytoremediation potential. The maximum Cd was observed at 60 days (32.05, 16.86, and 10.63%) of Cd application, compared to 15 (2.04, 0.60, and 1.17%) or 30 days (8.41, 3.93, and 4.20%, respectively), in a dose-dependent manner. The application of EDTA in the soil and foliar IAA enhanced the Cd accumulation in the plants at 15, 30, and 60 days of exposure, with maximum accumulation at 60 days. Exposed plants with foliar IAA application showed 64.82%, 33.77%, and 25.84% absorption at 50, 100, and 150 mg/kg, respectively. Apart from higher absorption, the cadmium translocation to the edible part of the plants ceased, i.e., the seeds had 0% accumulation. The interesting fact was recorded that efficient phytoremediation was recorded at 15 days of exposure, whereas maximum phytoremediation was recorded at 60 days of exposure. To minimize the stress, the host also produced stress-related metabolites (i.e., flavonoids, phenolics, proline, and sugar) and antioxidants (i.e., catalases and ascorbate peroxidases). From the current evidence, it could be assumed that the use of EDTA and IAA, along with hyperaccumulating plants, could be a possible green method to remediate Cd-contaminated soil efficiently in a short period of time.

Bioremediation and microbial-assisted phytoremediation of heavy metals by endophytic Fusarium species isolated from Convolvulus arvensis

Endophytic fungi are very prolific organisms associated with a wide range of host plants. Although they contribute to tolerance of their hosts against environmental stress, their role in response of plants to metal toxicity has not been thoroughly illuminated. Therefore, in this study, endophytic fungi were isolated from Convolvulus arvensis and their potential to bioremediate heavy metals from liquid media was assessed. Furthermore, the effect of the endophytic fungi on heavy metal tolerance, accumulation, and translocation by host plants were evaluated. The results revealed that Fusarium falciforme (MW898136) and Fusarium sp. (MW898137) possess a significant ability for bio-removal of cadmium from aqueous solutions as their tolerance indexes were >1. The bioaccumulation capacity reached 276.5 and 101.6 μg/g with bioremediation percentages of 82.4% and 76.5%, respectively. Fusarium falciforme (MW898136) filtrates, in the presence of cadmium, stimulated the growth of Triticum aestivum, and dramatically decreased cadmium bioaccumulation from 30.18 to 7.06 μg/g. The presence of F. falciforme (MW898136) in cadmium-stressed soil assisted Phaseolus vulgaris to produce more biomass, uptake higher amounts of cadmium by roots, and decreased the translocation of cadmium from roots to shoots. These results suggest that F. falciforme (MW898136) has the ability to immobilize cadmium and decrease its bioavailability in both liquid media and soil through absorption and/or accumulation.

Effects of different water conditions on the cadmium hyperaccumulation efficiency of Rorippa sylvestris (L.) Besser and Rorippa amphibia Besser.

Increasing the translocation and accumulation of cadmium (Cd) in Cd hyperaccumulator is an important technology to improve the phytoremediation efficiency of Cd-contaminated soil. In order to investigate the effects of different water conditions on the growth and Cd accumulation ability of Cd hyperaccumulators Rorippa sylvestris (L.) Besser and Rorippa amphibia Besser in Cd-polluted soil, clone seedlings of them were transplanted into pots filled with 50mgkg-1 Cd-contaminated soil and cultured with water conditions of soil relative water content (RWC) 35%, 55%, 75%, 95%, and flooding respectively. The results showed the following: with the increase of RWC, the height of R. sylvestris and R. amphibia increased gradually, the dry biomass of shoot and whole plant increased and reached the maximum in 95% and then decreased in flooding; the Cd concentrations in shoots of R. sylvestris and R. amphibia were more than 100mgkg-1 except for 35% and flooding; Cd bioconcentration factors (BCFs) of R. amphibia reached the maximum of 3.8870 in 75% and R. sylvestris reached the maximum of 3.2330 in 95%; sufficient water resulted in the decrease of photosynthetic rate due to more Cd accumulation. However, under flooding condition, because of the decrease of Cd bioavailability in soil, the accumulation of Cd in shoots declined and the net photosynthetic rate (Pn) enhanced slightly.