Translation Products Research Articles

Translation, transmission and indigenization of Christianity in nineteenth-century China: south-to-north travel of the disguised San Zi Jing by Medhurst

The Chinese traditional educational primer San Zi Jing (三字经) was immensely appreciated by Western missionaries, who frequently leveraged it to spur Chinese-language learning or to decipher Chinese thought. Walter Henry Medhurst (1796–1857), affiliated with the London Missionary Society (LMS), translated and reconstructed biblical scriptures into the format and style of the Chinese-language San Zi Jing, henceforth referred to as the “disguised San Zi Jing” in this article. The disguised San Zi Jing was initially published in Batavia in 1823 and reprinted in several locations, occasionally with minor modifications to its content. First, this study examines the complex qualities of the original San Zi Jing, which made it an ideal translation target, and the unique natures of the disguised San Zi Jing, which served as the ultimate translation product. Second, referencing Edward Said’s traveling theory, a diachronic investigation finds that the disguised San Zi Jing traveled in four stages, from Nanyang to Lingnan to Jiangnan and finally across Pan-China (more regions of China). Last, the article examines the indigenization process of the disguised San Zi Jing in space and content over time and analyzes the power dynamics underlying indigenization. The study contributes to translation history studies as well as studies of Christianity in China.

Examining student translators’ writing and translation products: quality, errors and self-perceived mental workload

ABSTRACT Although writing and translation are closely related text productions, their interface has rarely been studied in translator training. This study examined student translators’ writing and translation products in terms of their quality, errors and self-perceived mental workload. Data were collected from 11 intermediate-level translation students at a university in mainland China. They were asked to complete four tasks: L1 (Chinese) writing (L1W), L1 (English-Chinese) translation (L1T), L2 (English) writing (L2W), and L2 (Chinese-English) translation (L2T). The results of quantitative analyses revealed no significant linear correlation between self-perceived mental workload and quality/errors, but a significant effect of task type on mental workload. Errors most frequently occurred in translation and L2 text production. The L2W error rate predicted L2T scores and L2T error rates. A discrepancy was noted between the students’ self-evaluation and their actual performance. The findings of this study may help researchers and teachers gain a fine-grained understanding of students’ performance in writing and translation and shed light on the development of pedagogical measures in translator training.

Literature in and through Translation: Literary Translation as a Pedagogical Resource

This article is the revised version of the paper that I presented at the 5th APTIS (Association of Programmes in Translation and Interpreting Studies) 2023 conference (“The teaching and learning that matter today”), whose proceedings were never published. As a result of globalisation, the number of books requiring translation considerably increased. Nevertheless, readers do not always acknowledge translations as such, and literary translators do not generally obtain the recognition they deserve. Academia may be partly responsible for that: on the one side, indeed, literary translation is not as discussed as other topics within the broader field of Translation Studies; on the other, whilst teaching texts in translation is becoming increasingly common, translated literature is not generally considered as an academic discipline on its own. To promote a wider circulation and appreciation of translated literature in and beyond academia, translated literary texts could be systematically introduced into the curricula of courses in literature and literary translation. This could be achieved through the compilation and use of parallel corpora, namely collections of source texts and respective translations. In this light, this paper has two main objectives: explaining how courses in literature and literary translation could be taught using parallel corpora; showcasing the pedagogical advantages that such an approach may have on different levels. As for courses in literature it would provide students with an understanding of the mechanisms behind the production of literary translations and their relevance within the broader literary system. On what concerns courses in literary translation, it may represent a compromise between theory and practice, and between the research-orientated environment of academic settings and the commercially-orientated publishing industry. The study was conducted through the review of pedagogical practices and contexts where literary texts are taught in translation. The paper concludes with the observation that this corpus-based teaching approach may have some positive repercussions outside academia: it would not only contribute to a broader appreciation of translated literary texts among the general public but also foster a broader recognition of the role of the literary translator in shaping and constructing foreign literature.

New Literature from the Paris Disputation against the Talmud

Abstract In 1239, the convert Nicholas Donin submitted thirty-five articles against the Talmud to Pope Gregory IX. As a result, Christendom became aware of how Jews observed an oral law that was allegedly plagued with folly, blasphemy, and heresy. This triggered the infamous trial against the Talmud and resulted in the production of several Latin translations of rabbinical texts, including the compilation of the Extractiones de Talmud (1245). The present article mainly focusses on a short text taken from the manuscript Vatican City, Biblioteca Apostolica Vaticana, MS Vat. lat. 822 (folios 204ra–205ra), which includes a series of Talmudic passages that derive from texts which emerged from the Paris Disputation against the Talmud (1240–1245). From it, we are able to infer new information regarding the genesis of subsequent anti-Jewish works, including the Errores Iudaeorum by Thibaud de Sézanne and Passau Anonymous anti-Jewish sections. Additionally, we also provide the edition and translation of the text of the Vatican manuscript at the end of the article.

The authority of God takes precedence over Scripture and tradition

The divinely derived authority of the Bible to teach and guide, is somewhat mixed with the authority of traditions to interpret Scripture and document such interpretations outside Scripture. Tradition-based Bible translations pose a threat of elevating the authority of tradition over that of Scripture. All concerned traditions should not occupy or tame the authority of Scripture by producing Bibles attuned to tradition. The Scriptures were inspired by the Holy Spirit who inspires Bible readers to understand them. Tradition should not shape or formulate Scripture in accordance with its interpretive convictions. The Bible translations, produced under the auspices of specific traditions, should be exposed to guard the authority of Scripture not to be subdued by any tradition. The endless production of Bible translations has been considered to determine what could necessitate them. Among other findings, traditions have been found to be active in producing Bible translations, when their purview should be doing biblical interpretations and publish them within their traditions, and not to release them to the public as alternative Bibles or versions. The authority of God through the Holy Spirit, who inspired the prophets and apostles to write Scripture, is not transferable to any tradition. Regardless of the proliferation of tradition-based Bible translations, the authority to guide and teach believers, remains with God and the outlook of believers should be based on the supreme authority of God. The principle of Sola Scriptura is somewhat unsafe in the light of the production of tradition-orient Bible versions. The inspiration of the Bible by the Holy Spirit predates canonisation, redaction and restructuring of Scriptures. The production and publication of tradition-based Bibles with synthetic translation rules, poses a threat of diluting Scripture to align with specific traditions. Tradition is fallible and cannot be as authoritative as God. All authority to teach and guide continues to rest with the infallible God.Contribution: Continuous Reformation could regress to the outgrown pre-Reformation teachings that were subject to the authority of tradition, if the proliferation of tradition-based Bible translations is not noticed with a threat that they pose on the divinely derived authority of the Scriptures to teach and guide believing people. The gains of Reformers and Reformation should be guarded by being aware of Bible translation trends.

Landscape of Métis health and wellness: protocol for a scoping review

IntroductionIn Canada, Métis people are one of three distinct Indigenous peoples whose rights are recognised and affirmed in Section 35 of the federal Constitution Act, 1982. In line with Métis...

Molecular characterisation of a novel sadwavirus infecting cattleya orchids in Australia

The complete genome sequence of a novel sadwavirus infecting cattleya orchids in South East Queensland is described. Isometric virions of c. 27 nm diameter were observed in sap extracts viewed under a transmission electron microscope, and the genome sequence of this virus was determined by high-throughput sequencing. The viral genome consists of two RNA components, 5,910 and 4,435 nucleotides (nt) in length, each encoding a long polyprotein, with predicted cleavage sites at H/Y, E/G, Q/S, and Q/G for the RNA1 and T/G for the RNA2 translation products, respectively. RNA2 has an additional small ORF of 684 nt near the 3ʹ untranslated region. Phylogenetic analysis based on an amino acid sequence alignment of the Pro-Pol region suggested that this virus is most closely related to pineapple secovirus A, a member of the subgenus Cholivirus, but warrants classification as a member of a new species because it exhibited no more than 64% amino acid identity in pairwise sequence comparisons. Because of the prominent purple ringspots that were observed on the leaves of some of the plants, we propose the name “cattleya purple ringspot virus” for this virus (suggested species name: “Sadwavirus cattleyacola”).

Governance-oriented state translation program in the Zhenguan era of the Tang dynasty

ABSTRACT State translation program (STP) has existed since ancient times, and its manifestations, implementation modes, and governance functions have shown different characteristics in different political and historical periods. The histories, descriptions, and theories of translation in the Tang dynasty indicated that many influential inbound and outbound translation events during the Zhenguan era reflected the characteristics of STP. The state was the subject of translation activities, and through five means: nationalisation of translation institutions, intensification of resources, synergization of translation procedures, institutionalisation of translators’ identities, and classicisation of translation products, translation was elevated to a state act, which played the role of governance in maintaining imperial rule, stabilising social order, and constructing state image. The Zhenguan era has an essential position in the history of Chinese translation, and an examination of the types, characteristics, and functions of STP during this era will help reveal the form and mode of institutionalised translation and translation governance in ancient China, which can provide valuable experience and solutions for the implementation of translation governance in today’s states.

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats.

A GGGGCC (G4C2) hexanucleotide repeat expansion in C9ORF72 causes amyotrophic lateral sclerosis and frontotemporal dementia (C9ALS/FTD), while a CGG trinucleotide repeat expansion in FMR1 leads to the neurodegenerative disorder Fragile X-associated tremor/ataxia syndrome (FXTAS). These GC-rich repeats form RNA secondary structures that support repeat-associated non-AUG (RAN) translation of toxic proteins that contribute to disease pathogenesis. Here we assessed whether these same repeats might trigger stalling and interfere with translational elongation. We find that depletion of ribosome-associated quality control (RQC) factors NEMF, LTN1and ANKZF1 markedly boost RAN translation product accumulation from both G4C2 and CGG repeats while overexpression of these factors reduces RAN production in both reporter assays and C9ALS/FTD patient iPSC-derived neurons. We also detected partially made products from both G4C2 and CGG repeats whose abundance increased with RQC factor depletion. Repeat RNA sequence, rather than amino acid content, is central to the impact of RQC factor depletion on RAN translation-suggesting a role for RNA secondary structure in these processes. Together, these findings suggest that ribosomal stalling and RQC pathway activation during RAN translation inhibits the generation of toxic RAN products. We propose augmenting RQC activity as a therapeutic strategy in GC-rich repeat expansion disorders.

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Translational control exerts immediate effect on the composition, abundance, and integrity of the proteome. Ribosome-associated quality control (RQC) handles ribosomes stalled at the elongation and termination steps of translation, with ZNF598 in mammals and Hel2 in yeast serving as key sensors of translation stalling and coordinators of downstream resolution of collided ribosomes, termination of stalled translation, and removal of faulty translation products. The physiological regulation of RQC in general and ZNF598 in particular in multicellular settings is underexplored. Here we show that ZNF598 undergoes regulatory K63-linked ubiquitination in a CNOT4-dependent manner and is upregulated upon mitochondrial stresses in mammalian cells and Drosophila. ZNF598 promotes resolution of stalled ribosomes and protects against mitochondrial stress in a ubiquitination-dependent fashion. In Drosophila models of neurodegenerative diseases and patient cells, ZNF598 overexpression aborts stalled translation of mitochondrial outer membrane-associated mRNAs, removes faulty translation products causal of disease, and improves mitochondrial and tissue health. These results shed lights on the regulation of ZNF598 and its functional role in mitochondrial and tissue homeostasis.

The Use of Google Translate and U-Dictionary as Machine Translation for Translating Text: EFL College Student’s Preference and Perceptions

Several studies have investigated the effects of using Google Translate and U-Dictionary on translation products for English as a foreign language (EFL) student. However, students' preferences regarding these two machines’ translation for translating texts in EFL translation classes in Indonesia still seem relatively few and have not been much explored. This research aimed to determine which machine translation EFL college students prefer for translating their text. This study used a qualitative case study approach. In investigating students’ preferences, questionnaires, and in-depth interview were used as data collection tools. Seventeen students were selected by purposive sampling and were administered an online questionnaire. Then, students participated in in-depth interviews and obtained further information from an online questionnaire. We then analyzed the data using two techniques, including frequency counts for quantitative data and qualitative analysis through thematic analysis for qualitative data. The findings reveal that students prefer Google Translate over U-Dictionary for three reasons. First, they consider Google Translate as a complete machine translation with various features that help them translate text. Second, Google Translate increases student's knowledge of new vocabulary. Third, Google Translate makes the translation process more accessible for students. It is so easy to access. However, U-Dictionary also received high marks because students felt more confident in the accuracy of the U-Dictionary translation results. Therefore, it is believed that combining these two machines’ translations will make the student's translation process more accessible

Consumer focus group testing with stakeholders to generate an enteral resuscitation training flipbook for primary health center and first-level hospital providers in Nepal

IntroductionEnteral resuscitation (EResus) is operationally advantageous to intravenous resuscitation for burn-injured patients in some low-resource settings. However, there is minimal guidance and no training materials for EResus tailored to non-burn care providers. We aimed to develop and consumer-test a training flipbook with doctors and nurses in Nepal to aid broader dissemination of this life-saving technique. Materials and methodsWe used individual cognitive interviews with Nepali (n = 12) and international (n = 4) burn care experts to define key elements of EResus and specific concepts for its operationalization at primary health centers and first-level hospitals in Nepal. Content, prototype illustrations, and wireframe layouts were developed and revised with the burn care experts. Subsequently, eight consumer testing focus groups with Nepali stakeholders (5–10 people each) were facilitated. Prompts were generated using the Questionnaire Appraisal System (QAS) framework. The flipbook was iteratively revised and tested based on consumer feedback organized according to the domains of clarity, assumptions, knowledge/memory, and sensitivity/bias. Results and discussionThe flipbook elements were iterated until consumers made no additional requests for changes. Examples of consumer inputs included: clarity—minimize medical jargon, add shrunken organs and wilted plants to represent burn shock; assumptions—use locally representative figures, depict oral rehydration salts sachet instead of a graduated bottle; knowledge/memory—clarify complex topics, use Rule-of-9 s and depict approximately 20% total body surface area to indicate the threshold for resuscitation; sensitivity/bias—reduce anatomic illustration details (e.g. urinary catheter placement, body contours). ConclusionStakeholder engagement, consumer testing, and iterative revision can generate knowledge translation products that reflect contextually appropriate education materials for inexperienced burn providers. The EResus Training Flipbook can be used in Nepal and adapted to other contexts to facilitate the implementation of EResus globally.

Transcript Profiles of Microglia/Macrophage Cells at the Borders of Chronic Active and Subpial Gray Matter Lesions in Multiple Sclerosis.

Microglia/macrophages line the border of demyelinated lesions in both cerebral white matter and the cortex in the brains of multiple sclerosis patients. Microglia/macrophages associated with chronic white matter lesions are thought to be responsible for slow lesion expansion and disability progression in progressive multiple sclerosis, whereas those lining gray matter lesions are less studied. Profiling these microglia/macrophages could help to focus therapies on genes or pathways specific to lesion expansion and disease progression. We compared the morphology and transcript profiles of microglia/macrophages associated with borders of white matter (WM line) and subpial gray matter lesions (GM line) using laser capture microscopy. We performed RNA sequencing on isolated cells followed by immunocytochemistry to determine the distribution of translational products of transcripts increased in WM line microglia. Cells in the WM line appear activated, with shorter processes and larger cell bodies, whereas those in the GM line appear more homeostatic, with smaller cell bodies and multiple thin processes. Transcript profiling revealed 176 genes in WM lines and 111 genes in GM lines as differentially expressed. Transcripts associated with immune activation and iron homeostasis were increased in WM line microglia, whereas genes belonging to the canonical Wnt signaling pathway were increased in GM line microglia. We propose that the mechanisms of demyelination and dynamics of lesion expansion are responsible for differential transcript expression in WM lines and GM lines, and posit that increased expression of the Fc epsilon receptor, spleen tyrosine kinase, and Bruton's tyrosine kinase, play a key role in regulating microglia/macrophage function at the border of chronic active white matter lesions. ANN NEUROL 2024;95:907-916.

Translating Noncanonical Ancillary Qur’anic Oppositions Into English: An Etiotypological Analysis

Translating the sacred in a so-called divine language has been shown to be so difficult and challenging a task for translators of the Bible or the Qur’an. The human reproduction of the divine product(ion) has biblically and qur’anically been found to raise (in)soluble challenges and (in)surmountable hurdles in bicultural and cross-cultural communication and transfer in translation. One great challenge or big obstacle thereof is the (un)transferability of noncanonical ancillary oppositions from SL (QA) to TL (E) which is sought to be explored and investigated by the current study from a linguistic-translational perspective. Based on corpus data across several languages, noncanonical ancillary opposition has been shown to co-occur in preponderant syntactic frames loaned from fellow categories, such as coordination, subordination, transition, and so forth, to perform cross-categorial discourse functions in canonical, semicanonical, and noncanonical configurations, and to entertain a propensity and penchant for special multi-principled and rule-governed sequences based on morphology, gender, agency, and so on. The specific objectives of this study are to compare and contrast SL and TL frequent frameworks and ordering sequences in the process and product of translation. It was found that a number of these frames and functions have gone untended and unrendered by the two Qur’an translators under scrutiny due to their incognizance of colligational forms and discursive functions as translation units of functional sentence perspective contributing to the semantic ends of intraversially sentential structures. It was also revealed that SL paradigmatic lexical choices were too semantically distinct and complex to lexicalize into TL.

A familial missense variant in the Alzheimer’s disease gene SORL1 impairs its maturation and endosomal sorting

The SORL1 gene has recently emerged as a strong Alzheimer’s Disease (AD) risk gene. Over 500 different variants have been identified in the gene and the contribution of individual variants to AD development and progression is still largely unknown. Here, we describe a family consisting of 2 parents and 5 offspring. Both parents were affected with dementia and one had confirmed AD pathology with an age of onset > 75 years. All offspring were affected with AD with ages at onset ranging from 53 years to 74 years. DNA was available from the parent with confirmed AD and 5 offspring. We identified a coding variant, p.(Arg953Cys), in SORL1 in 5 of 6 individuals affected by AD. Notably, variant carriers had severe AD pathology, and the SORL1 variant segregated with TDP-43 pathology (LATE-NC). We further characterized this variant and show that this Arginine substitution occurs at a critical position in the YWTD-domain of the SORL1 translation product, SORL1. Functional studies further show that the p.R953C variant leads to retention of the SORL1 protein in the endoplasmic reticulum which leads to decreased maturation and shedding of the receptor and prevents its normal endosomal trafficking. Together, our analysis suggests that p.R953C is a pathogenic variant of SORL1 and sheds light on mechanisms of how missense SORL1 variants may lead to AD.

Abstract A038: Non-canonical MHC class I-associated antigens in pancreatic cancer

Abstract Translation of the ostensibly non-coding genome in cancer can generate novel peptides capable of presentation by major histocompatibility complex class I (MHC-I, HLA-I in humans). These non-canonical peptide sources can broaden the landscape of potentially targetable antigens in low-to-intermediate mutational burden malignancies, typified by pancreatic ductal adenocarcinoma (PDA). However, non-canonical MHC-I-associated peptides (ncMAPs) have yet to be explored in pancreatic cancer. We purified the malignant compartment from low tumor cellularity PDA specimens using patient-derived organoids (PDOs) and developed a personalized proteogenomics pipeline coupled with high-depth immunopeptidomics to deeply characterize the repertoire of HLA:peptide targets presented specifically on malignant cells. We demonstrate that ncMAPs are abundant on PDA and predominate over mutation-derived neoepitopes in the detectable immunopeptidome. While emerging evidence in other tumor types has implicated dysregulated translation of non-canonical open reading frames (ncORFs), it is currently unknown to what extent these translation products are truly cancer-restricted and how effectively the resulting ncMAPs can elicit a cytolytic T lymphocyte (CTL) response. To investigate the cancer-specificity of non-canonical peptides, we developed a translation-centric analysis pipeline that examines ncORF expression across a range of healthy tissues, including healthy thymus. We provide evidence that ~30% of ncMAPs exhibit cancer-restricted translation patterns, and a substantial subset of these are shared among pancreatic cancer patients with the appropriate HLA. To investigate immunogenicity, we employed a highly sensitive ex vivo platform to prime and expand antigen-specific T cells. We demonstrate that PDA-restricted ncMAPs are highly immunogenic, on par with or even exceeding the immunogenicity observed with mutation-derived peptides or tumor-associated antigens assayed using the same platform. These findings uncover a critical role for dysregulated translation in pancreatic cancer as a potential source for recurrent cancer-restricted epitopes capable of recognition by cytotoxic T cells. We envision that this novel class of antigens will accelerate ongoing efforts to treat pancreatic cancer patients with vaccines and cell-based therapies. Citation Format: Zackery A. Ely, Zachary J. Kulstad, Eva K. Verzani, Jennifer G. Abelin, Sudarsana Addepalli, Karl R. Clauser, Marta Casarrubios, Kevin S. Kapner, Miles P. Agus, Connor J. Hennessey, Sine R. Hadrup, Susan Klaeger, Jennifer Su, Alex M. Jaeger, Brian M. Wolpin, Srivatsan Raghavan, Philip D. Greenberg, Andrew J. Aguirre, Steven A. Carr, Tyler Jacks, William A. Freed-Pastor. Non-canonical MHC class I-associated antigens in pancreatic cancer [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr A038.

Khayyam’s Quatrains as Fitzgerald’s Rubẚiyat: Translation as Ideological Misrepresentation

Research studies from around the globe on Omar Khayyam’s Persian quatrains and their translation into English by the poet, writer, and translator Edward Fitzgerald, are in abundance. Researchers are, in general, in praise of the translation and give credit to Fitzgerald for making Khayyam a world-renowned poet. However, the translation has rarely been approached from a socio-political perspective or a look into Fitzgerald’s ideological manipulation of the original. The present research study investigates two issues with Fitzgerald’s translation- ideological manipulation and selective translation. The study also looks into Khayyam’s life and his works. It probes into the effects this translation left on the literary scene. The study involves a comparative literary translation analysis to compare and contrast the elements found in Fitzgerald’s translation and two Arabic translations. Employing Lefevere’s (1992) theory of ‘translation as rewriting,’ this paper assesses the extent to which a translator’s ideology can lead to a misrepresented product of translation (Lefevere, 1992). The study adopts textual analysis as a research method to capture the epicurean elements recurrently emphasized by Fitzgerald in his translation.

An asymmetric Janus membrane with anti-bacteria adhesion and rapid hemostasis properties for wound healing

Currently, commercial intraoral dressings have limitations as they only serve a single purpose and fail to meet various needs, such as hemostasis, wound protection, and dressing fixation. Here, we successfully fabricated a multifunctional polycaprolactone-chitosan Janus membrane (PCJM) with simultaneous hydrophobic and hydrophilic properties. The hydrophobic layer consists of a dense and disordered polycaprolactone, preventing bacterial adhesion, resisting fouling, and enhancing mechanical strength. The hydrophilic layer is a highly porous chitosan sponge, which facilitates cell and protein adhesion, as well as rapid absorption of blood and activation of the coagulation process. These results are confirmed by hemostasis experiments, which demonstrate that the PCJM had superior coagulation ability compared to commercial gelatin sponges and gauze, and by an early gingival healing model of beagle tooth extraction wounds, which exhibited excellent performance in promoting wound healing. Our work demonstrates the multifunctional PCJM wound dressing not only protects wounds from bacterial infections but also facilitates rapid hemostasis and early healing of beagle gingival soft tissue, which holds significant potential for clinical translation and large-scale production.

Lifting the curtain on the emergency department crisis: a multi-method reception study of Larry Saves the Canadian Healthcare System

BackgroundDespite growing evidence of the potential of arts-based modalities to translate knowledge and spark discussion on complex issues, applications to health policy are rare. This study explored the potential of a research-based theatrical video to increase public capacity and motivation to engage with the complex issues that make Emergency Department wait times such an intractable problem.MethodsLarry Saves the Canadian Healthcare System is a digital musical micro-series developed from extensive research examining system-level causes of Emergency crowding and the ineffectiveness of prevailing approaches. We released individual episodes and a revised full-length version on YouTube, using organic promotion strategies and paid advertising. We used YouTube Analytics to track views, engagement and viewer demographics, and content-analyzed viewer comments. We also conducted five university-based screenings; 92 students completed questionnaires, rating Larry on 16 descriptors using a 7-point Likert scale.ResultsFrom June 2022 through May 2023, Larry garnered over 100,000 views (76,752 of the full-length version, 35,535 of episodes), 1329 likes, 2780 shares, and 139 comments. Views and watch time were higher among women and positively associated with age. Among YouTube comments, the predominating themes were praise for the video and criticism of the healthcare system. Many commenters applauded the show’s accuracy, humor, and/or resonance with their experience; several shared healthcare horror stories. Students overwhelmingly agreed with all positive and disagreed with all negative descriptors, and nearly unanimously deemed the video informative, thought-provoking, and entertaining. Most also affirmed that it had increased their knowledge, interest, and confidence to participate in discussions about healthcare issues. Neither gender, primary language, nor employment in healthcare predicted ratings, but graduate students and those 25+ years old evaluated the video most positively.DiscussionThese findings highlight the promise of research-informed musical satire to inform and invigorate discourse on an urgent health policy problem. Larry has reached tens of thousands of viewers, garnered excellent feedback, and received high student ratings. Further research should directly assess educational and behavioural outcomes and explore what facilitative strategies could maximize this knowledge translation product’s potential to foster informed, impactful policy dialogue.

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Cellular proteostasis requires transport of polypeptides across membranes. Although defective transport processes trigger cytosolic rescue and quality control mechanisms that clear translocases and membranes from unproductive cargo, proteins that are synthesized within mitochondria are not accessible to these mechanisms. Mitochondrial-encoded proteins are inserted cotranslationally into the inner membrane by the conserved insertase OXA1L. Here, we identify TMEM126A as a OXA1L-interacting protein. TMEM126A associates with mitochondrial ribosomes and translation products. Loss of TMEM126A leads to the destabilization of mitochondrial translation products, triggering an inner membrane quality control process, in which newly synthesized proteins are degraded by the mitochondrial iAAA protease. Our data reveal that TMEM126A cooperates with OXA1L in protein insertion into the membrane. Upon loss of TMEM126A, the cargo-blocked OXA1L insertase complexes undergo proteolytic clearance by the iAAA protease machinery together with its cargo.