Event Abstract Back to Event A murine model of atherosclerosis, frequency and phenotype of the B cell subsets involved Diana Castaño1, Hector Rincón-Arévalo1, Janny Villa-Pulgarín2, Robinson Ramírez-Pineda2, Gloria Vásquez1 and Lina Yassin3* 1 Universidad de Antioquia, Grupo de Inmunología Celular e Inmunogética, Instituto de Investigaciones Médicas, Facultad de Medicina, Colombia 2 Grupo de Inmunomodulación, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Colombia 3 Grupo de Ciencias Básicas, Facultad de Medicina, Universidad CES, Colombia Atherosclerosis is a chronic inflammatory disease of the artery wall. Several studies have described a role for B cells in atherosclerosis. Adoptive transfer of follicular B cells promoted atheromas, whereas B1a cells protected atherosclerosis onset. Currently, the mechanisms responsible for this modulation are unknown. To characterize the B cell subsets involved in a murine model of atherosclerosis, C57BL/6 wild-type and atherogenic apolipoprotein-E knockout (Apoe-/-) mice were fed with high fat (HFD) or regular diet for 12 weeks. At that time, Apoe-/- mice developed overwhelming lesions in the aortic sinus, compared with wild-type ones. The study of splenic cells according CD19, CD21, CD23, and CD5 expression, showed not differences in the frequency of follicular, marginal zone and transitional 2 (T2) B cells between wild-type and Apoe-/- mice, before and after the pro-atherogenic diet. In Apoe-/- mice with HFD a significant reduction of CD19 expression was observed in total B cells that was more evident in T2 cells. Also, in this group of mice the percentage of CD5+ T2 cells was decreased, and in this subset CD21 expression was decreased while CD40 was increased. There were not differences in IgM, IgD, CD80 and CD86 expression in B subsets among the groups. Since, CD19, CD5 and CD40 are associated with the regulatory function of B cells, the next step in this line of investigation will be the evaluation of the regulatory function of these T2 cells in mice with established atherosclerosis. Acknowledgements This work is funded by COLCIENCIAS (grant number 111554531390) and Sostenibilidad 2013-2014 Universidad de Antioquia References Nakashima Y, Plump AS, Raines EW, Breslow JL and Ross R. ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb (1994) 14:133-40. Petro JB, Gerstein RM, Lowe J, Carter RS, Shinners N and Khan WN. Transitional type 1 and 2 B lymphocyte subsets are differentially responsive to antigen receptor signaling. J Biol Chem (2002) 277:48009-19. Matsushita T, Horikawa M, Iwata Y and Tedder TF. Regulatory B cells (B10 cells) and regulatory T cells have independent roles in controlling experimental autoimmune encephalomyelitis initiation and late-phase immunopathogenesis. J Immunol (2010) 185:2240-52. Keywords: B cells, Atherosclerosis, apolipoprotein-E, transitional-2, CD19 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Castaño D, Rincón-Arévalo H, Villa-Pulgarín J, Ramírez-Pineda R, Vásquez G and Yassin L (2013). A murine model of atherosclerosis, frequency and phenotype of the B cell subsets involved. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00670 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Lina Yassin, Grupo de Ciencias Básicas, Facultad de Medicina, Universidad CES, Medellin, Colombia, lyassin@ces.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Diana Castaño Hector Rincón-Arévalo Janny Villa-Pulgarín Robinson Ramírez-Pineda Gloria Vásquez Lina Yassin Google Diana Castaño Hector Rincón-Arévalo Janny Villa-Pulgarín Robinson Ramírez-Pineda Gloria Vásquez Lina Yassin Google Scholar Diana Castaño Hector Rincón-Arévalo Janny Villa-Pulgarín Robinson Ramírez-Pineda Gloria Vásquez Lina Yassin PubMed Diana Castaño Hector Rincón-Arévalo Janny Villa-Pulgarín Robinson Ramírez-Pineda Gloria Vásquez Lina Yassin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.