A portable and low-cost system prototype for glucose detector based on LMP91000EV M potentiostat has been created. Characterization of Screen-Printed Carbon Electrodes (SPCE) without modification (bare SPCE) and modified nickel oxide (NiO/SPCE) was carried out with a commercial potentiostat to see the effect of nickel oxide in detecting glucose. Through the electrochemical impedance spectroscopy method, the Rct value of 1,276.79 Ω is obtained for NiO/SPCE and 429.06 Ω for bare SPCE, so that NiO/SPCE has a slower electron transfer rate. Meanwhile, through the cyclic voltammetry method, the surface-active electrode area is 7.1×10-2cm2 for NiO/SPCE and 6.9×10-2 cm2 for bare SPCE, so that NiO/SPCE is more sensitive in detecting glucose. When the glucose concentration is varied, the Limit of Detection (LOD) and Limit of Quantitation (LOQ) values of NiO/SPCE are smaller, specifically 1.807 mM and 6.024 mM than bare SPCE, specifically 2.629 mM and 8.762 mM, so NiO/SPCE is more sensitive. When the scan rate is varied, the gradient value of NiO/SPCE is smaller, specifically -8.14×10-4 mA s/mV than bare SPCE, specifically -9.62×10-4 mA s/mV, so NiO/SPCE is not more sensitive. Next, comparing a prototype system that Trans Impedance Amplifier (TIA) gain is varied and a commercial potentiostat. As a result, the cyclic voltammogram per cycle on commercial potentiostat is more stable. The smaller the TIA gain on the system prototype, the more stable it is, this is because the noise that is amplified is getting smaller.
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