Transgenic Varieties Research Articles

Genetic variation assessment of stacked-trait transgenic maize via conventional breeding

BackgroundThe safety assessment and control of stacked transgenic crops is increasingly important due to continuous crop development and is urgently needed in China. The genetic stability of foreign genes and unintended effects are the primary problems encountered in safety assessment. Omics techniques are useful for addressing these problems. The stacked transgenic maize variety 12–5 × IE034, which has insect-resistant and glyphosate-tolerant traits, was developed via a breeding stack using 12–5 and IE034 as parents. Using 12–5 × IE034, its parents (12–5 and IE034), and different maize varieties as materials, we performed proteomic profiling, molecular characterization and a genetic stability analysis.ResultsOur results showed that the copy number of foreign genes in 12–5 × IE034 is identical to that of its parents 12–5 and IE034. Foreign genes can be stably inherited over different generations. Proteomic profiling analysis found no newly expressed proteins in 12–5 × IE034, and the differences in protein expression between 12 and 5 × IE034 and its parents were within the range of variation of conventional maize varieties. The expression levels of key enzymes participating in the shikimic acid pathway which is related to glyphosate tolerance of 12–5 × IE034 were not significantly different from those of its parents or five conventional maize varieties, which indicated that without selective pressure by glyphosate, the introduced EPSPS synthase is not has a pronounced impact on the synthesis of aromatic amino acids in maize.ConclusionsStacked-trait development via conventional breeding did not have an impact on the genetic stability of T-DNA, and the impact of stacked breeding on the maize proteome was less significant than that of genotypic differences. The results of this study provide a theoretical basis for the development of a safety assessment approach for stacked-trait transgenic crops in China.

FvC5SD overexpression enhances drought tolerance in soybean by reactive oxygen species scavenging and modulating stress-responsive gene expression.

Overexpression of FvC5SD improves drought tolerance in soybean. Drought stress is one of the most important abiotic stress factors that influence soybean crop quality and yield. Therefore, the creation of drought-tolerant soybean germplasm resources through genetic engineering technology is effective in alleviating drought stress. FvC5SD is a type of C-5 sterol desaturase gene that is obtained from the edible fungus Flammulina velutipes. This gene has good tolerance to the effects of stresses, including drought and low temperature, in yeast cells and tomato. In this study, we introduced the FvC5SD gene into the soybean variety Shennong9 through the Agrobacterium-mediated transformation of soybean to identify drought-tolerant transgenic soybean varieties. PCR, RT-PCR, and Southern blot analysis results showed that T-DNA was inserted into the soybean genome and stably inherited by the progeny. The ectopic expression of FvC5SD under the control of a CaMV 35S promoter in transgenic soybean plants enhanced the plant's tolerance to dehydration and drought. Under drought conditions, the transgenic plants accumulated lower levels of reactive oxygen species and exhibited higher activities and expression levels of enzymes and cell than wild-type soybean. iTRAQ analysis of the comparative proteomics showed that some exogenous genes coding either functional or regulatory proteins were induced in the transgenic lines under drought stress. FvC5SD overexpression can serve as a direct and efficient target in improving drought tolerance in soybean and may be an important biotechnological strategy for trait improvement in soybean and other crops.

Overexpression of Sorghum plasma membrane-bound Na+/H+ antiporter-like protein (SbNHXLP) enhances salt tolerance in transgenic groundnut (Arachis hypogaea L.)

Soil salinity and water-deficit conditions often affect crop productivity in groundnut. Therefore, developing transgenic groundnut that can grow under such abiotic stress conditions is crucial to stabilize its yield. Sodium proton antiporter-like protein (NHXLP) is a plasma membrane-bound protein associated with Na+ exclusion and helps to maintain ion homeostasis under saline conditions. In the present study, salt tolerant transgenic groundnut variety JL-24 was developed by expressing SbNHXLP gene isolated from Sorghum bicolor. Molecular analysis of transgenics by PCR and Southern blot confirmed the integration of SbNHXLP gene. SbNHXLP expression at the transcript level was checked by reverse transcriptase (RT)-PCR. Homozygous T2 lines along with wild-type (WT) plants were evaluated for 150 mM NaCl stress tolerance. Biochemical analysis of transgenics under salt stress revealed higher chlorophyll content, superoxide dismutase, and catalase activities, accumulation of proline, and K+ accompanied by lower Na+ accumulation compared to WT plants. Additionally, transgenics displayed higher biomass and pod yield when compared with WT plants under stress. Our findings indicate that overexpression of SbNHXLP gene in groundnut results in enhanced tolerance to salinity stress. This highlights the potential of SbNHXLP as a target candidate gene to impart salt stress tolerance in groundnut. A sodium proton antiporter-like protein isolated from Sorghum (SbNHXLP) was overexpressed in groundnut and stably integrated. Transgenics displayed higher chlorophyll, proline, K+, and better yields than WT plants under salt stress.

Evaluation of wheat events transformed with the p5cs gene under conditions of water stress

The enzyme ∆1-pyrroline-5-carboxylate synthetase (P5CS), encoded by the p5cs gene, is the rate-limiting step for proline biosynthesis, which acts as an osmoprotectant. The launch of transgenic commercial varieties depends on physiological and phenotypic characterization of events. The objective of this study was to evaluate five p5cs gene events (591, 14, 326, 164, 312), through physiological analyses and production rates during droughts. Plants of events and irrigated (IC) and stressed controls (SC) in flowering stage were submitted to 16 days of complete water restriction. The following elements were evaluated: relative water content (RWC); malondialdehyde concentration (MDA%); membrane stability index (MSI%); proline content; and production rates. As a result, the effect of water stress was only perceived after 12 days of water restriction with a decrease in the RWC values. Transgenic plants showed higher proline levels on the 16th day, compared to the SC (33 to 62%). The levels of MDA were lower on the 12th and 16th days for all the plants evaluated, but the MSI were similar to the IC for the events 591, 326, and 164, demonstrating the osmoprotectant effect. However, higher levels of proline did not result in higher production rates in the transgenic plants.

Helicoverpa zea (Lepidoptera: Noctuidae) Preference for Plant Structures, and Their Location, Within Bt Cotton Under Different Nitrogen and Irrigation Regimes

Helicoverpa zea Boddie is a common economic pest of cotton (Gossypium hirsutum L.), including transgenic cotton varieties that express Bacillus thuringiensis (Bt). Helicoverpa zea oviposition is similar in Bt and non-Bt cotton, but behavior of H. zea larvae can be different in the presence of Bt, with neonates moving away from terminals faster in single-toxin Bt than non-Bt cotton or avoiding Bt-treated diet in the lab. We quantified H. zea oviposition and larval distribution on structures within cotton plants in small plot experiments of Cry1Ac + Cry1F cotton for 2 yr under different irrigation and nitrogen treatments. More eggs were oviposited on plants receiving nitrogen application during 2016 and on leaves in the top section of irrigated plants during 2017, but other treatment effects on eggs or larvae were minimal. Helicoverpa zea eggs were most common on leaves in the top third of plants at position zero and middle section of cotton plants throughout the season, but some oviposition occurred on fruiting structures as well. First and second instars were more common on squares in the top section of plants during 2016 and bolls in the middle and lower sections during 2017 due to oviposition lower in the canopy during 2017. During both years, third through fifth instars were more common on bolls in the middle and lower section of plants closer to the main stem. These findings have resistance management implications as extended larval feeding on bolls could optimize nutrition, decrease Bt susceptibility, and potentially influence behavioral resistance.

Loss of GLK1 transcription factor function reveals new insights in chlorophyll biosynthesis and chloroplast development.

Birch (Betula platyphylla × B. pendula) is an important tree for landscaping due to its attractive white bark and straight trunk. In this study, we characterized a T-DNA yellow-green leaf mutant, yl. We identified six insertion sites (ISs) in the mutant by genome resequencing and found a 40-kb deletion containing BpGLK1 around IS2 on chromosome 2. Complementation experiments with the yl mutant and repression of BpGLK1 in wild-type plants confirmed that BpGLK1 was responsible for the mutated phenotype. Physiological and ultrastructural analyses showed that the leaves of the yl mutant and BpGLK1-repression lines had decreased chlorophyll content and defective chloroplast development compared to the wild-type. Furthermore, the loss function of BpGLK1 also affected photosynthesis in leaves. Transcriptomics, proteomics, and ChIP-PCR analysis revealed that BpGLK1 directly interacted with the promoter of genes related to antenna proteins, chlorophyll biosynthesis, and photosystem subunit synthesis, and regulated their expression. Overall, our research not only provides new insights into the mechanism of chloroplast development and chlorophyll biosynthesis regulated by BpGLK1, but also provides new transgenic birch varieties with various levels of yellowing leaves by repressing BpGLK1 expression.

Limited effect of planting transgenic rice on the soil microbiome studied by continuous 13CO2 labeling combined with high-throughput sequencing.

The planting of transgenic rice has aroused ongoing controversy, due to the public anxiety surrounding the potential risk of transgenic rice to health and the environment. The soil microbial community plays an important environmental role in the plant-soil-microbe system; however, few studies have focused on the effect of transgenic rice on the soil rhizospheric microbiome. We labeled transgenic gene rice (TT51, transformed with Cry1Ab/1Ac gene), able to produce the Bt (Bacillus thuringiensis) toxin, its parental variety (Minghui 63), and a non-parental variety (9931) with 13CO2. The DNA of the associated soil rhizospheric microbes was extracted, subjected to density gradient centrifugation, followed by high-throughput sequencing of bacterial 16S rRNA gene. Unweighted unifrac analysis of the sequencing showed that transgenic rice did not significantly change the soil bacterial community structure compared with its parental variety. The order Opitutales, affiliated to phylum Verrucomicrobia and order Sphingobacteriales, was the main group of labeled bacteria in soil planted with the transgenic and parental varieties, while the orders Pedosphaerales, Chthoniobacteraceae, also affiliated to Verrucomicrobia, and the genus Geobacter, affiliated to class Deltaproteobacteria, dominated in the soil of the non-parental rice variety. The non-significant difference in soil bacterial community structure of labeled microbes between the transgenic and parental varieties, but the comparatively large difference with the non-parental variety, suggests a limited effect of planting transgenic Bt rice on the soil microbiome.

Screening of salt tolerant transgenic and non-transgenic cotton varieties under various levels of NaCl induced salinity stress

Screening of salt tolerant transgenic and non-transgenic cotton varieties under various levels of NaCl induced salinity stress

Effect of plating density, amino acid and osmoticum on transformation efficiency of papaya (Carica papaya L.)

Papaya suffers colossal losses due to Papaya Ring Spot Virus (PRSV) disease. PRSV is responsible for about 70% loss in papaya production worldwide. This disease is present all over the world wherever papaya is grown. Transgenic papaya varieties namely SunUp and Rainbow have been developed and commercialized in the USA using Pathogen Derived Resistance (PDR). The present investigation reports a successful gene delivery system in an Indian papaya variety employing PDR. Agro-infected immature young globular embryos of papaya cv. Pusa Delicious were co-cultivated with A. tumefaciens harboring a hairpin loop construct of a truncated PRSV coat protein gene. The Agro-infected explants were plated at a density of 2–3, in the presence of 100 mg L−1 L-cysteine in dark for 72 h. Transformed plants maturation under the influence of 45 mg L−1 PEG produced 4 independent transgenic events which were characterized by PCR and data sequencing.

First transgenic trait for control of plant bugs and thrips in cotton.

BACKGROUNDPlant bugs (Lygus spp.) and thrips (Thrips spp.) are two of the most economically important insect pest groups impacting cotton production in the USA today, but are not controlled by current transgenic cotton varieties. Thus, seed or foliar‐applied chemical insecticides are typically required to protect cotton from these pest groups. Currently, these pests are resistant to several insecticides, resulting in fewer options for economically viable management. Previous publications documented the efficacy of transgenic cotton event MON 88702 against plant bugs and thrips in limited laboratory and field studies. Here, we report results from multi‐location and multi‐year field studies demonstrating efficacy provided by MON 88702 against various levels of these pests.RESULTSMON 88702 provided a significant reduction in numbers of Lygus nymphs and subsequent yield advantage. MON 88702 also had fewer thrips and minimal injury. The level of control demonstrated by this transgenic trait was significantly better compared with its non‐transgenic near‐isoline, DP393, receiving insecticides at current commercial rates.CONCLUSIONThe level of efficacy demonstrated here suggests that MON 88702, when incorporated into existing IPM programs, could become a valuable additional tool for management of Lygus and thrips in cotton agroecosystems experiencing challenges of resistance to existing chemical control strategies. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

GENETIC SOURCES OF RESISTANCE TO ROOT ROTS CYCORY FROM DIFFERENT ECOLOGICAL AND GEOGRAPHICAL ORIGIN

The main type of root chicory diseases are root rot of root crops. In unfavorable years, they affect up to 40-50% of root crops, which are culled from the test weight and are not paid for when the crop is delivered to the processing enterprises. The most common lesions of the roots of chicory various pathogenic species of fungi: Phoma rostrupii Sacc., Botrytis cinerea (P.) Fr. J, Erwiria carotovora (Jones) Holt. Production is in dire need of new varieties of root chicory, resistant to root rot both during the growing season and during long-term storage. To develop transgenic varieties among the many varieties to allocate genetic sources of this feature below in further breeding work to fix him in generations. The article presents the data on the study of the defeat of root crops of root chicory varieties of different ecological and geographical origin of different types of root rot in the collection nursery (including the provocative background) for 2015-2017 in the NonChernozem zone of the Russian Federation in the Rostov region of the Yaroslavl oblast. The best resistance to root rot were varieties Luxor (Holland) and Harpachi (Hungary), the roots of which did not have signs of root rot, varieties Petrovsky (Russia), Wixor (Holland) and Sleszka (Czech Republic) were weakly affected (less than 2%). During the long winter storage, the best on this basis, proven varieties Harpachi (Hungary), Spicak (Czech Republic) and Luxor (the Netherlands), which had been preserved. These varieties can serve as a source of resistance to root rot and can be involved in the selection process as a donor of this feature.

Analytical Enzymatic Saccharification of Lignocellulosic Biomass for Conversion to Biofuels and Bio-Based Chemicals

Lignocellulosic feedstocks are an important resource for biorefining of renewables to bio-based fuels, chemicals, and materials. Relevant feedstocks include energy crops, residues from agriculture and forestry, and agro-industrial and forest-industrial residues. The feedstocks differ with respect to their recalcitrance to bioconversion through pretreatment and enzymatic saccharification, which will produce sugars that can be further converted to advanced biofuels and other products through microbial fermentation processes. In analytical enzymatic saccharification, the susceptibility of lignocellulosic samples to pretreatment and enzymatic saccharification is assessed in analytical scale using high-throughput or semi-automated techniques. This type of analysis is particularly relevant for screening of large collections of natural or transgenic varieties of plants that are dedicated to production of biofuels or other bio-based chemicals. In combination with studies of plant physiology and cell wall chemistry, analytical enzymatic saccharification can provide information about the fundamental reasons behind lignocellulose recalcitrance as well as about the potential of collections of plants or different fractions of plants for industrial biorefining. This review is focused on techniques used by researchers for screening the susceptibility of plants to pretreatment and enzymatic saccharification, and advantages and disadvantages that are associated with different approaches.

Activity of antioxidant enzymes in chloroplasts of transgenic plants of wheat with double-stranded RNA-suppressor of the prolindehydrogenase gene

Aim. To investigate the activity of antioxidant enzymes in chloroplasts of transgenic wheat plants variety Zymoyarka containing a double-stranded RNA-suppressor of the proline dehydrogenase gene. Methods. Spectrophotometrical determination of the activity of enzymes. Results. Under physiological conditions, the activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in transgenic plants significantly exceeded these indices in non-transgenic forms. Under soil drought, the activity of SOD in transgenic plants decreased significantly and amounted to only 70 % of the activity of this enzyme in control plants, the activity of APX was 12 % lower than that of control. The water deficit increased sharply (4–5 times) the level of free L-proline in transgenic wheat plants with reduced activity pdh. Conclusions. Biotechnological plants of wheat with increased activity of SOD and APX under optimum conditions can be characterized by increased adaptive plasticity compared to plants of original variety with lower activity of the enzymes. The increased activity of antioxidant enzymes may be a prerequisite for increasing the tolerance of these plants to stressors of different origins.
 Keywords: Triticum aestivum L., transgenic plants, double-stranded RNA-suppressor of the proline dehydrogenase gene, L-proline, antioxidant enzymes.

Genetically Modified Wheat, Wheat Intolerance, and Food Safety Concerns

Wheat intolerance is a common problem for certain individuals. A gluten-free diet is the only option for people with wheat-associated disorders (WAD) to manage their condition. The elimination of immunogenic proteins from the wheat is the most appropriate approach to ameliorate the symptoms of affected individuals, while also meeting their nutritional requirements. RNA interference technology can be exploited to silence the expression of gliadins to produce a wheat variety lacking the immunogenic proteins associated with WAD, but there are challenges before implementation of transgenic varieties in the market will occur. This review is focussed on RNA interference approaches acquired to produce wheat that patients with different WAD can tolerate. The authors also discuss the advantages and disadvantages of current omics approaches that are being used to validate the food safety issues related to the applicability and clinical relevance of genetically modified wheat.

The Integration of Algal Carbon Concentration Mechanism Components into Tobacco Chloroplasts Increases Photosynthetic Efficiency and Biomass

Increasing the productivity of crops is a major challenge in agricultural research. Given that photosynthetic carbon assimilation is necessary for plant growth, enhancing the efficiency of photosynthesis is one strategy to boost agricultural productivity. The authors attempted to increase the photosynthetic efficiency and biomass of tobacco plants by expressing individual components of the Chlamydomonas reinhardtii carbon concentration mechanism (CCM) and integrating them into the chloroplast. Independent transgenic varieties are generated accumulating the carbonic anhydrase CAH3 in the thylakoid lumen or the bicarbonate transporter LCIA in the inner chloroplast membrane. Independent homozygous transgenic lines showed enhanced CO2 uptake rates (up to 15%), increased photosystem II efficiency (by up to 18%), and chlorophyll content (up to 19%). Transgenic lines produced more shoot biomass than wild-type and azygous controls, and accumulated more carbohydrate and amino acids, reflecting the higher rate of photosynthetic CO2 fixation. These data demonstrate that individual algal CCM components can be integrated into C3 plants to increase biomass, suggesting that transgenic lines combining multiple CCM components could further increase the productivity and yield of C3 crops.

Efficiency of cotton bollworm (Helicoverpa armigera H\xfcbner) control of different Bt cotton varieties in North China

BackgroundThe cotton bollworm (Helicoverpa armigera) is one of cotton’s most destructive insect pests in terms of yield and quality. Since 1997, China has grown commercially available transgenic Bacillus thuringiensis (Bt) cotton. We aimed to investigate the variation in resistance of transgenic Bt cotton varieties to cotton bollworm in North China.MethodsPopulations of cotton bollworm were monitored from 2008 to 2015 in environments where Bt cotton was planted adjacent to other non-Bt crops. The study included 197 Bt cotton varieties planted in 42 counties/locations in three provinces (Hebei, Shandong and Henan) of North China, which were evaluated through field investigations, bioassays, and enzyme-linked immunosorbent assays (ELISA).ResultsThe average number of cotton bollworms never exceeded the action threshold (10 larvae per 100 cotton plants), however, their number reached 19.55 per 100 cotton plants in 2011. The ratios of damaged plants to total Bt cotton stem-tips, buds, and bolls was low except in 2010, when the ratios reached 1.82%, 2.09%, and 10.63%, respectively. The results of bioassay showed that the corrected mortality were higher at the second generation cotton bollworm stage than the third and fourth germination stages. Totally, Bt protein content declined sharply at the seedling stage from 2008 to 2015.ConclusionsThis study indicated that almost all Bt cotton varieties were capable to effectively control the populations of cotton bollworm in North China.

An Update on Genetic Modification of Chickpea for Increased Yield and Stress Tolerance.

Chickpea is a highly nutritious grain legume crop, widely appreciated as a health food, especially in the Indian subcontinent. The major constraints on chickpea production are biotic (Helicoverpa, bruchid, aphid, ascochyta) and abiotic (drought, heat, salt, cold) stresses, which reduce the yield by up to 90%. Various strategies like conventional breeding, molecular breeding, and modern plant breeding have been used to overcome these problems. Conventionally, breeding programs aim at development of varieties that combine maximum number of traits through inter-specific hybridization, wide hybridization, and hybridization involving more than two parents. Breeding is difficult in this crop because of its self-pollinating nature and limited genetic variation. Recent advances in in vitro culture and gene technologies offer unique opportunities to realize the full potential of chickpea production. However, as of date, no transgenic chickpea variety has been approved for cultivation in the world. In this review, we provide an update on the development of genetically modified chickpea plants, including those resistant to Helicoverpa armigera, Callosobruchus maculatus, Aphis craccivora, as well as to drought and salt stress. The genes utilized for development of resistance against pod borer, bruchid, aphid, drought, and salt tolerance, namely, Bt, alpha amylase inhibitor, ASAL, P5CSF129A, and P5CS, respectively, are discussed.

Feral rice from introgression of weedy rice genes into transgenic herbicide-resistant hybrid-rice progeny

Pollen-mediated transgenic flow of herbicide resistance occurs bidirectionally between transgenic cultivated rice and weedy rice. The potential risk of weedy traits introgressing into hybrid rice has been underestimated and is poorly understood. In this study, two glufosinate-resistant transgenic rice varieties, hybrid rice (F1), and their succeeding generations (F2-F4) were planted for 3 years in field plots free of weedy rice adjacent to experimental weedy-rice fields. Weedy-rice-like (feral) plants that were both glufosinate-resistant and had red-pericarp seed were initially found only among the F3 generations of the two glufosinate-resistant transgenic hybrid cultivars. The composite fitness (an index based on eight productivity and weediness traits) of the feral progeny was significantly higher than that of the glufosinate-resistant transgenic hybrid (the original female parent of the feral progeny) under monoculture common garden conditions. The hybrid rice progeny segregated into individuals of variable height and extended flowering. The hybrid rice F2 generations had higher outcrossing rates by pollen reception (0.96-1.65%) than their progenitors (0.07-0.98%). The results show that herbicide-resistant weedy rice can rapidly arise by pollen-mediated gene flow from weedy to transgenic hybrid rice, and their segregating pollen-receptive progeny pose a greater agro-ecological risk than transgenic varieties. The safety assessment and management regulations for transgenic hybrid rice should take into account the risk of bidirectional gene flow.

Transgenic zero-erucic and high-oleic mustard oil improves glucose clearance rate, erythrocyte membrane docosahexaenoic acid content and reduces osmotic fragility of erythrocytes in male Syrian golden hamsters

Brassica juncea, the Indian mustard variety has high erucic acid (22:1 n-9) in its oil, which causes several deleterious effects. The Centre for Genetic Manipulation of Crop Plants (India) has developed a zero-erucic and high-oleic acid transgenic mustard variety having 67% oleic acid, which is almost equivalent to that of olive oil, i.e. 71%. Therefore, we assessed its impact on erythrocyte osmotic fragility, fluidity and activities of membrane-bound enzymes and insulin sensitivity. 40 male Syrian golden hamsters of 6–8 weeks age, were divided into five groups, consisting of 8 hamsters in each and fed diet containing any one of the oils, i.e. groundnut (GNO), conventional mustard (OCM), low-erucic mustard (OLM), zero-erucic high-oleic transgenic mustard (OTM) and olive (OLO) at 10% level for 16 weeks. At the end, compared to OLO group, OTM-fed hamsters resisted osmotic shock-induced erythrocyte-haemolysis, which corroborated with higher docosahexaenoic acid (DHA; 22:6 n-3) levels in their erythrocyte membranes. However, it did affect neither the fluidity nor the activities of membrane-bound enzymes. Although fasting plasma glucose, insulin and free fatty acid levels were comparable among the various groups; during glucose challenge, OTM diet-fed animals displayed higher disposal rate of circulatory glucose, without altering the insulin levels, when compared to the conventional mustard; OCM. In conclusion, the consumption of oil from zero-erucic high-oleic transgenic mustard improved the DHA content of erythrocyte membrane, which possibly resisted haemolysis and enhanced glucose clearance during glucose overload. However, it did not affect the activities of erythrocyte membrane-bound enzymes and fluidity compared to olive oil.

INNOVATION SOLUTIONS FOR THE IMPORT PHASE OUT PURPOSES

The data of the Far East Region on the use of new highly effective developments of research institutions in solving problems of import substitution in agricultureare given.As a result of the activity of the five scientific institutions of the region, 162 varieties of various agricultural crops have been introduced in the Register of Selection Achievements, including: 49 – soybean, 40 –fruit and berry crops, 25 – potato and vegetables, 15 – perennial grasses, 33 –cereal and groats crops. All created varieties are resistant to environmental conditions and pests, they have a high potential for productivity, which is implemented in difficult weather and climatic conditions with limited thermal resources. The soybean varieties of the Far Eastern selection are highly yield in the limited heat conditions. In the last five years the variety assortment was renewed by 90 per cent. In this period Far Eastern breeders delivered in to production 25 high yield not transgenic varieties, 17 of them was selected in the all-Russian research organization institute of the soybean (RROI of the soybean) , them also very popular in Krasnoyarsk and Altai territories. The seed treatment by the fertilizers produced on the basis of high-activity stock culture combining with active phosphorus and potassium favors the yield increase to 462.97 …2020.25 pounds hereby increase the payback of 2.20 pounds fertilizers active ingredient to 2,8…12,3 kg of the grain.