Introduction: Research shows high rates of discrimination against the transgender population in healthcare centers, which has negative consequences for their quality of life. There are few studies in this area that focus upon the experiences of trans men, although, they tend to experience higher levels of violence and stigmatization in healthcare settings. Method: We explored the perceptions of 14 Chilean trans men regarding discrimination in healthcare centers from an ethnographic approach, with the aim of analyzing their experiences in these facilities, and identifying their needs in relation to healthcare. Results: The majority of trans men perceived health centers as a source of discrimination. The most important expressions of discrimination involve questioning their gender identity, and disregarding their social names and pronouns. In view of this, interviewees placed fear of discrimination as the main factor preventing them from being attended at healthcare centers. On the other hand, having access to health workers who are trained in providing care to the transgender population, being provided with information, and the possibility of receiving psychosocial support are the main factors promoting their attendance at these facilities. Discussion: The study has implications both for health professionals and public policies focused on the transmasculine community. Recommendations are made for professionals and administrative staff to provide respectful and sympathetic care in order to generate spaces free of discrimination that encourage attendance at healthcare centers, improving the welfare of trans men.