Transforming Activity Research Articles

Guiding the HBO1 complex function through the JADE subunit.

JADE is a core subunit of the HBO1 acetyltransferase complex that regulates developmental and epigenetic programs and promotes gene transcription. Here we describe the mechanism by which JADE facilitates recruitment of the HBO1 complex to chromatin and mediates its enzymatic activity. Structural, genomic and complex assembly in vivo studies show that the PZP (PHD1-zinc-knuckle-PHD2) domain of JADE engages the nucleosome through binding to histone H3 and DNA and is necessary for the association with chromatin targets. Recognition of unmethylated H3K4 by PZP directs enzymatic activity of the complex toward histone H4 acetylation, whereas H3K4 hypermethylation alters histone substrate selectivity. We demonstrate that PZP contributes to leukemogenesis, augmenting transforming activity of the NUP98-JADE2 fusion. Our findings highlight biological consequences and the impact of the intact JADE subunit on genomic recruitment, enzymatic function and pathological activity of the HBO1 complex.

Evidence of nitrogen inputs affecting soil nitrogen purification by mediating root exudates of salt marsh plants

Salt marsh has an important ‘purification’ role in coastal ecosystems by removing excess nitrogen that could otherwise harm aquatic life and reduce water quality. Recent studies suggest that salt marsh root exudates might be the ‘control centre’ for nitrogen transformation, but empirical evidence is lacking. Here we sought to estimate the direction and magnitude of nitrogen purification by salt marsh root exudates and gain a mechanistic understanding of the biogeochemical transformation pathway(s). To achieve this, we used a laboratory incubation to quantify both the root exudates and soil nitrogen purification rates, in addition to the enzyme activities and functional genes under Phragmites australis populations with different nitrogen forms addition (NO3−, NH4+ and urea). We found that NO3− and urea addition significantly stimulate P. australis root exudation of total acids, amino acids, total sugars and total organic carbon, while NH4+ addition only significantly increased total acids, amino acids and total phenol exudation. High total sugars, amino acids and total organic carbon concentrations enlarged nitrogen purification potential by stimulating the nitrogen purifying bacterial activities (including enzyme activities and related genes expression). Potential denitrification rates were not significantly elevated under NH4+ addition in comparison to NO3− and urea addition, which should be ascribed to total phenol self-toxicity and selective inhibition. Further, urea addition stimulated urease and protease activities with providing more NH4+ and NO2− substrates for elevated anaerobic ammonium oxidation rates among the nitrogen addition treatments. Overall, this study revealed that exogenous nitrogen could increase the nitrogen purification-associated bacterial activity through accelerating the root exudate release, which could stimulate the activity of nitrogen transformation, and then improve the nitrogen removal capacity in salt marsh.

To the issue of development of entrepreneurial state activity

The article deals with theoretical and applied issues of expanding the diversity of forms of state participation inthe economy within the framework of a new methodological approachto the development of economic state activity. The main establishedscientific concepts of state entrepreneurial activity are considered, the contentof the concept of “state entrepreneurship” is analysed, its modernforms are highlighted. The special emphasis in the work ismade on the study of entrepreneurial activity of the staterelated to the use of joint-stock property in the marketsector of the economy. The tendencies of transformation and developmentof entrepreneurial state activity in the conditions of new geopoliticaland economic reality are shown.

Organomagnesia: Reversibly High Carbon Dioxide Uptake by Magnesium Pyrazolates.

A series of new pyrazolate and mixed pyrazolate/pyrazole magnesium complexes is described and their reactivity toward carbon dioxide is examined. The dimeric complex [Mg(pzt Bu, t Bu)2]2 inserts CO2 instantly and quantitatively forming the tetrameric complex [Mg(CO2·pzt Bu, t Bu)2]4 and monomeric donor-stabilized [Mg(CO2·pzt Bu, t Bu)2(thf)2]. Complexes of the type [Mgx(pzR,R)2 x(HpzR,R)y]n (R = iPr, tBu) engage in similar insertion reactions involving dissociation of the carbamic acid HOOCpzR,R. Even solid polymeric derivatives [Mg(pzR,R)2]n (R = Me, H) react instantaneously and exhaustively with CO2, the resulting [Mg(CO2·pz)2]m featuring a CO2 capacity of 35.7wt% (8.2mmol g-1). All described magnesium pyrazolates display completely reversible CO2 uptake in solution and in the solid state, respectively, as monitored via VT 1H NMR and in situ FTIR spectroscopy as well as thermogravimetric analysis. Fluorinated [Mg2(pzCF3,CF3)4(thf)3] does not yield any isolable CO2 insertion product but exhibits the highest activity in the catalytic transformation of epoxides and CO2 to cyclic carbonates.

Transformation of the Forest Litter Microbial Community Introduced in to the Agrocenosis Soil

The long-term use of pesticides in the forest nurseries of the Sverdlovsk region has led to a decrease in the quality of Scots pine (Pinus sylvestris L.) planting material. As a way to reduce pesticide stress and create optimal conditions suitable for the conifers growth, a once use of forest litter (20 kg/m2) was proposed. This substrate is easier to obtain from a plantation near to the forest agrocenosis. However, by something cases, forest litter may be obtained from locations that are geographically far from the forest nursery. The article presents the results of a study the possibility of applying forest litter obtained from places located at different distances from the forest nursery in the bioremediation of pesticide-contaminated soil and improving the quality of Scots pine planting material. In the field experiment, samples of the forest litter were taken near two forest nurseries and introduced into the soil of the experimental plots before sowing pine. Part of the plots contained roundup (3 l a. s./ha), the other remained without treatment. In each nursery, plots were created containing both litter from a nearby stand and from a more remote forest area. It was found that the peak of the transforming activity of the microbiocenosis in the introduced forest litter and decreasing of the pesticide contamination, most likely, will be observed in the first stages of the microbiocenosis introduction. The observed effect takes place regardless of the distance from the application site where the forest substrate was obtained.

Everyone can draw: An inclusive and transformative activity for conceptualization of topographic anatomy.

Anatomical drawing traditionally involves illustration of labeled diagrams on two-dimensional surfaces to represent topographical features. Despite the visual nature of anatomy, many learners perceive that they lack drawing skills and do not engage in art-based learning. Recent advances in the capabilities of technology-enhanced learning have enabled the rapid and inexpensive production of three-dimensional anatomical models. This work describes a "drawing on model" activity in which learners observe and draw specific structures onto three-dimensional models. Sport and exercise sciences (SES, n = 79) and medical (MED, n = 156) students at a United Kingdom medical school completed this activity using heart and femur models, respectively. Learner demographics, their perceptions of anatomy learning approaches, the value of the activity, and their confidence in understanding anatomical features, were obtained via validated questionnaire. Responses to 7-point Likert-type and free-text items were analyzed by descriptive statistics and semi-quantitative content analysis. Learners valued art-based study (SES mean = 5.94 SD ±0.98; MED = 5.92 ± 1.05) and the "drawing on model" activity (SES = 6.33 ± 0.93; MED = 6.21 ± 0.94) and reported enhanced confidence in understanding of cardiac anatomy (5.61 ± 1.11), coronary arteries (6.03 ± 0.83), femur osteology (6.07 ± 1.07), and hip joint muscle actions (5.80 ± 1.20). Perceptions of learners were independent of both their sex and their art-based study preferences (p < 0.05). Themes constructed from free-text responses identified "interactivity," "topography," "transformative," and "visualization," as key elements of the approach, in addition to revealing some limitations. This work will have implications for anatomy educators seeking to engage learners in an inclusive, interactive, and effective learning activity for supporting three-dimensional anatomical understanding.

Cocrystal of Naringenin and Norfloxacin: Crystal Transformation, Solubility, and Antibacterial and Anticancer Activities

Cocrystal of Naringenin and Norfloxacin: Crystal Transformation, Solubility, and Antibacterial and Anticancer Activities

Cooperative roles of twinning, transformation and strain partitioning on the excellent cryogenic strength-toughness synergy of dual-heterogeneous metastable high entropy alloys

In a metastable Fe50Mn30Co10Cr10 high entropy alloy (HEA), a dual-heterogeneous structure, encompassing both grain size and dislocation heterogeneity, has been constructed to obtain excellent cryogenic mechanical performance by varying the annealing temperature (630 and 740 °C) following severe deformation. The HS-630 sample, consisting of recovered coarse-grains (CG), recrystallized fine-grains (FGs) and un-recrystallized ultrafine-grains (UFGs), possesses higher yield strength (YS) of ∼965 MPa, ultimate tensile strength (UTS) of ∼1680 MPa and uniform elongation (UEL) of ∼45 % when compared to the fully-recrystallized HS-740 sample at liquid nitrogen temperature (LNT). The difference in YS is primarily caused by the grain boundary and dislocation strengthening of UFGs, whereas the higher ductility of the HS-630 sample is associated with the hierarchical activation of transformation- and twinning-induced plasticity (TRIP and TWIP) effects, and compatible strain partitioning between heterogeneous domains. The dual-heterogeneity results in different critical stresses of ε-phase nucleation in heterogeneous domains, and promotes sustainable TRIP effect in CGs and the emergence of TWIP effect in UFGs with strain progressing. Moreover, the strain of the ductile transformed ε-phase is mainly accommodated by non-basal slip, which contributes to the strain hardening in the later deformation stage. Furthermore, the higher cryogenic impact toughness of the HS-630 sample can be attributed to the synergistic effects of the fracture mode, the transformation characteristics, and the heterogeneous strain partitioning.

Variation in Catalytic Efficacies of a 2D pH-Stable MOF by Altering Activation Methods.

Although it is well-known that the Lewis acidity of Metal-Organic Frameworks (MOFs) can effectively enhance their catalytic activity in organic transformations, access to these Lewis-acidic sites remains a key hurdle to widespread applications of Lewis-acidic catalysis by MOFs. Easy accessibility of strong Lewis acidic sites onto 2D MOFs by using proper activation methods can be a cornerstone in attaining desired catalytic performance. Herein, we report a new 2D chemically stable MOF, IITKGP-60, which displayed excellent framework robustness over a wide pH range (2-12). Benefiting from the abundant open metal sites (OMSs) and framework robustness, the catalytic activity of the developed material was explored in one-pot three-component Strecker reaction and Knoevenagel condensation reaction. Moreover, the developed catalyst is superior in catalyzing the reactions involving sterically hindered substrate (1-naphthaldehyde) with high turnover number. A comparative catalytic study was conducted using different activation methods (chloroform and methanol exchanged activated samples), highlighting the significant effect of activation methods on its catalytic performances. The sustainable synthetic pathway under solvent-free conditions for a broad scope of substrates using low catalyst loading and excellent recyclability made the developed pH-stable framework a promising heterogeneous catalyst.

Biotransformation of Ginsenoside Rb1 to Ginsenoside Rd and 7 Rare Ginsenosides Using Irpex lacteus with HPLC-HRMS/MS Identification.

The biotransformation of ginsenosides using microorganisms represents a promising and ecofriendly approach for the production of rare ginsenosides. The present study reports on the biotransformation of ginsenoside Rb1 using the fungus Irpex lacteus, resulting in the production of ginsenoside Rd and seven rare ginsenosides with novel structures. Employing high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry, the identities of the transformation products were rapidly determined. Two sets of isomers with molecular weights of 980.56 and 962.55 were discovered among the seven rare ginsenosides, which were generated through the isomerization of the olefin chain in the protopanaxadiol (PPD)-type ginsenoside skeleton. Each isomer exhibited characteristic fragment ions and neutral loss patterns in their tandem mass spectra, providing evidence of their unique structures. Time-course experiments demonstrated that the transformation reaction reached equilibrium after 14 days, with Rb1 initially generating Rd and compound 5, followed by the formation of other rare ginsenosides. The biotransformation process catalyzed by I. lacteus was found to involve not only the typical deglycosylation reaction at the C-20 position but also hydroxylation at the C-22 and C-23 positions, as well as hydrogenation, transfer, and cyclization of the double bond at the C-24(25) position. These enzymatic capabilities extend to the structural modification of other PPD-type ginsenosides such as Rc and Rd, revealing the potential of I. lacteus for the production of a wider range of rare ginsenosides. The transformation activities observed in I. lacteus are unprecedented among fungal biotransformations of ginsenosides. This study highlights the application of a medicinal fungi-based biotransformation strategy for the generation of rare ginsenosides with enhanced structural diversity, thereby expanding the variety of bioactive compounds derived from ginseng.

A Critical Discussion of the Relationship Between Processes of Spatial Transformation and Youth Gang Activity in Denver

Denver, Colorado is one of the most rapidly gentrifying cities in the United States. Case studies of gentrifying cities, including Chicago and Los Angeles demonstrate a relationship between urban restructuring and the emergence of youth gangs. However, this phenomenon is under-investigated in Denver. Drawing on an integrative literature review, this paper engages in a critical discussion of the relationship between processes of spatial transformation and youth gang activity in Denver. The contestation of urban space is identified as a central contributor to rising rates of gang criminality in the city’s gentrifying areas. These findings highlight the significance of community activism in popular resistance to gentrification and the protection of residents’ rights to the city.

Empowering education through digital transformation: Confronting educational wastage in basic education schools in Jordan

Activating digital transformation in basic education holds the potential to effectively address educational wastage by leveraging innovative technologies and strategies to optimize teaching and learning processes, enhance student engagement and outcomes and mitigate inefficiencies in educational practices. The objective of this research was to explore the feasibility of implementing digital transformation as a solution to address educational wastage in basic education schools within the Irbid Governorate. The study adopted a descriptive and analytical methodology employing a random sampling technique to select a sample of 270 principals and teachers from elementary education schools in Irbid Governorate. Data collection was facilitated through the utilization of a questionnaire as the research instrument. The validity and reliability of the instrument underwent a meticulous examination and validation process through a series of rigorous steps. The findings revealed several obstacles hindering the activation of digital transformation in basic education including the absence of integrated digital systems that function cohesively, limited utilization of modern techniques, technologies and teaching methods and insufficient teacher proficiency in technology usage and training. The study recommends overcoming these challenges by transitioning the school environment into a digital ecosystem, supporting the professional growth of teachers in scientific, educational and technical domains, establishing systems and regulations to enhance the school environment and improving teaching methods and learning strategies.

Digital transformation and innovation activities: evidence from publicly-listed firms in China

This study investigates the relationship between digital transformation (DT) and corporate innovation activities. By studying the publicly-listed firms in China, we find that firms with higher levels of DT tend to spend more on research and development (R&D) and tend to generate more innovation outputs. The impact of DT on R&D expenditures is particularly pronounced for firms with more limited disclosure of information, more complex organizational structures, and for those who do not receive government sponsorship. We find no evidence that DT affects innovation through alleviating financial constraints or enhancing operating performance. Collectively, these findings offer novel and practical insights into the interaction between digitization and firms’ innovation.

Research on the Activation Path of Employees’ Openness to Digital Transformation: A New Impetus to Realize Enterprises’ Digital Transformation for Sustainable Development

Digital transformation is an important strategic decision for the sustainable development of enterprises, which helps enterprises achieve sustainable performance, sustainable management, and even sustainable business models. While there have been a number of useful studies discussing the impetus of digital transformation, most of them have neglected the role from employees, especially their attitudes and perceptions towards transformation. Focusing on employees’ openness to digital transformation, we selected six antecedents to construct a configuration model, using fsQCA and NCA methods to explore the complex causal relationship between each antecedent and openness to digital transformation. This aims to derive the activation paths for employees’ openness to digital transformation, so as to help the enterprises’ digital transformation and further achieve sustainable development. Through the data analysis of 462 employees of Chinese enterprises, the following conclusions are drawn: firstly, single antecedents are not necessary conditions for high/non-high digital transformation openness activation; secondly, there are three configurations associated with the high openness activation of digital transformation: a comprehensive collaboration type under internal environment dominance (S1a + S1b), the consideration of both an internal and external environment type under mindfulness characteristics’ dominance (S2), and a mindful substitution type under internal environment dominance (S3); and finally, there are four configurations associated with non-high digital transformation openness activation and an asymmetric relationship exists with the high digital transformation openness activation path.

Valuation of the significant hypoglycemic activity of black currant anthocyanin extract by both starch structure transformation and glycosidase activity inhibition

The objective of this study was to investigate the impact of anthocyanin-rich black currant extract (BCE) on the structural properties of starch and the inhibition of glycosidases, gathering data and research evidence to support the use of low glycemic index (GI) foods. The BCE induced a change in the starch crystal structure from A-type to V-type, resulting in a drop in digestibility from 81.41 % to 65.57 %. Furthermore, the inhibitory effects of BCE on glycosidases activity (α-glucosidase: IC50 = 0.13 ± 0.05 mg/mL and α-amylase: IC50 = 2.67 ± 0.16 mg/mL) by inducing a change in spatial conformation were confirmed through in vitro analysis. The presence of a 5′-OH group facilitated the interaction between anthocyanins and receptors of amylose, α-amylase, and α-glucosidase. The glycosyl moiety enhanced the affinity for amylose yet lowered the inhibitory effect on α-amylase. The in vivo analysis demonstrated that BCE resulted in a reduction of 3.96 mM·h in blood glucose levels (Area Under Curve). The significant hypoglycemic activity, particularly the decrease in postprandial blood glucose levels, highlights the potential of utilizing BCE in functional foods for preventing diabetes.

Insights into the oral microbiota in human systemic cancers.

The oral cavity stands as one of the pivotal interfaces facilitating the intricate interaction between the human body and the external environment. The impact of diverse oral microorganisms on the emergence and progression of various systemic cancers, typified by oral cancer, has garnered increasing attention. The potential pathogenicity of oral bacteria, notably the anaerobic Porphyromonas gingivalis and Fusobacterium nucleatum, has been extensively studied and exhibits obvious correlation with different carcinoma types. Furthermore, oral fungi and viruses are closely linked to oropharyngeal carcinoma. Multiple potential mechanisms of oral microbiota-induced carcinogenesis have been investigated, including heightened inflammatory responses, suppression of the host immune system, influence on the tumor microenvironment, anti-apoptotic activity, and promotion of malignant transformation. The disturbance of microbial equilibrium and the migration of oral microbiota play a pivotal role in facilitating oncogenic functions. This review aims to comprehensively outline the pathogenic mechanisms by which oral microbiota participate in carcinogenesis. Additionally, this review delves into their potential applications in cancer prevention, screening, and treatment. It proves to be a valuable resource for researchers investigating the intricate connection between oral microbiota and systemic cancers.

Dakwah Transformasional : Merespon Tren Sekulerisme Di Kalangan Gen Z

In the modern era, which is increasingly influenced by dominant secular culture, Generation Z faces significant challenges in maintaining and developing their spiritual and religious values. In this context, transformative preaching emerges as a promising approach to respond to this secular trend in a relevant and meaningful way. This research aims to explore the potential of transformative preaching in responding to the secular trend among Generation Z. We seek to understand how transformative preaching can assist Generation Z in finding meaning, depth, and spiritual direction in their lives, which are saturated with secular influences. The research methodology employed is qualitative, involving in-depth interviews and participatory observation with a sample group of Generation Z actively engaged in transformative preaching activities. Data analysis is conducted using an inductive approach to identify patterns and key findings. The main findings indicate that transformative preaching holds great potential in helping Generation Z overcome the challenges of secularism. The inclusive and open approach of transformative preaching creates a supportive environment for Generation Z to explore and deepen their understanding of spirituality. Additionally, the development of moral values and the utilization of social media and digital technology have proven effective in forming inclusive and supportive communities. This research makes a significant contribution to understanding the role of transformative preaching in responding to secular trends among Generation Z. These findings can aid in the development of more effective and relevant preaching programs to guide Generation Z in their search for meaning and spiritual direction in their complex lives.

Modulation of plasticity by crystalline precipitates in CuZr metallic glasses

Amorphous/crystalline composites consist of crystalline phases embedded in an amorphous matrix that offer the possibility for synthesizing materials with tailored properties. Here, molecular dynamics simulations were performed to study the plastic behavior of CuZr metallic glasses (MGs) with CuZr B2 spherical precipitates under tensile tests. Shear transformation zones were observed to nucleate at the MG/B2 interface, a phenomenon that was more remarkable at larger precipitate sizes. Furthermore, large precipitates acted as barriers for shear band (SB) propagation. To overcome such barriers, and owing to the lack of phase transformation and plastic activity in the CuZr B2 structures, SBs displaced the precipitates, leading to increased stress in the sample. In addition, the degree of strain localization was higher when the precipitates were closer to each other. Thus, the plasticity in our MG composites was modulated by the size and distribution of the precipitates.

Recording and classifying MET receptor mutations in cancers.

Tyrosine kinase inhibitors (TKI) directed against MET have been recently approved to treat advanced non-small cell lung cancer (NSCLC) harbouring activating MET mutations. This success is the consequence of a long characterization of MET mutations in cancers, which we propose to outline in this review. MET, a receptor tyrosine kinase (RTK), displays in a broad panel of cancers many deregulations liable to promote tumour progression. The first MET mutation was discovered in 1997, in hereditary papillary renal cancer (HPRC), providing the first direct link between MET mutations and cancer development. As in other RTKs, these mutations are located in the kinase domain, leading in most cases to ligand-independent MET activation. In 2014, novel MET mutations were identified in several advanced cancers, including lung cancers. These mutations alter splice sites of exon 14, causing in-frame exon 14 skipping and deletion of a regulatory domain. Because these mutations are not located in the kinase domain, they are original and their mode of action has yet to be fully elucidated. Less than five years after the discovery of such mutations, the efficacy of a MET TKI was evidenced in NSCLC patients displaying MET exon 14 skipping. Yet its use led to a resistance mechanism involving acquisition of novel and already characterized MET mutations. Furthermore, novel somatic MET mutations are constantly being discovered. The challenge is no longer to identify them but to characterize them in order to predict their transforming activity and their sensitivity or resistance to MET TKIs, in order to adapt treatment.

First row transition metal catalyzed small molecule activation for sustainable organic transformation: Synergy between nature and chemist’s toolbox

In this review we have tried to present our view on the synergy between nature and chemists’ toolbox toward discovery of new reactions. Toward the search for new chemical reactivity, not only chemists learn from nature but nature’s evolutionary pathway perhaps has footprints of chemist’s innovative discoveries and beyond. Taking CO2 as a representative example of an abundant energy rich small molecule resource, we have tried to explore the probable strategies of activating the molecule using first row transition metal catalysts for sustainable organic transformation. Our study has tried to unfold nature’s strategy of CO2 activation and recycling which eventually motivated chemists toward discovery of new chemical reactions and further inspired to expand their toolbox for discovering new reactivities beyond the natural enzyme repertoire. The basic chemical CO2 activation strategy which have been focused in this review are using CO2 as a C1 building block in organic synthesis; catalytic reductive formylation and methylation of amines; carboxylation reactions via CO2 insertion. Additionally, carbonylation reactions with CO2 as CO surrogate has also been discussed. The focus of this review is to correlate nature’s chemical strategies with chemist’s approach to understand the concept involved in the chemical activation and utilization process of CO2 for unlocking new reaction paradigm, both for chemists’ and for nature’s catalytic repertoire.