Transect Method Research Articles

Factors Affecting the Winter Distribution of Birds in Forests at the Eastern Edge of Europe

We show for the first time the results of a study into the spatial distribution of birds in forests at the eastern edge of Europe (Republic of Tatarstan, Russia) and changes from early to late winter. A transect method was used to census randomly selected plots spread over a large geographical area in the winters 2018/9–2022/3. We used regression and ordination methods to assess the influence of key environmental factors on species richness, total density of birds, biological diversity, and the probabilities of occurrence of individual species. The most abundant bird species in early winter was the Willow Tit, and in the late winter was the Common Redpoll. Compared with the end of the 20th century, the number of wintering bird species has increased in the study area, likely due to climate warming. Species richness, total density, and the Shannon Index of diversity were higher in early winter than in late winter. Species richness and the Shannon Index were also higher at low elevations and in the west of the study region. Our research shows strong ecological-geographical differences in the preferences of individual bird species in the studied forests. However, almost without exception, birds had a higher probability of occurrence at lower elevation and toward the west.

Abundance of humpback whales in the eastern Indian sector of the Southern Ocean in 2018/19 using opportunistic sighting survey data with a note on the occurrence of other cetaceans

Past commercial whaling depleted the humpback whale in the Southern Ocean; however, its population has recovered since hunting was terminated in 1966. Information on abundance is essential not only managing target animals but understanding the ecosystem because the species is one of the main consumers of Antarctic krill. Abundance of this species has not been reported in the eastern Indian sector (80–150°E) of the Southern Ocean since the mid-2000s. A multidisciplinary ecosystem survey focusing on Antarctic krill was conducted in the area by the Japanese research vessel Kaiyo-maru in 2018/19 austral summer. An opportunistic cetacean sighting survey (platform of opportunity (POP) survey) was conducted as part of the survey. Four baleen and five toothed whale species were sighted during the sighting survey. Humpback whales (113 schools/178 animals) were most frequently observed, followed by fin whales (15/36). Abundance of humpback whales was estimated as 22,395 individuals (CV = 0.270, 95 % confidence intervals: 13,310–37,663) based on the design-based line transect method. Krill consumption by humpback whales in this area was tentatively estimated as 1.336 million tons, corresponding to 30.9 % of krill biomass, although the amount varied greatly depending on the consumption rate’s assumption. The estimated abundance was comparable with previous estimates, however, there are some caveats in interpreting the results. As no approaching whale sightings were made during this POP survey, the proportion of sightings of unidentified large whales was high, possibly leading to an underestimation of the abundance. Dedicated sighting surveys are desirable to obtain more accurate and unbiased abundance estimates, especially for management purposes, although the results of this study could be used for ecological studies in combination with other data sets recoded by the multidisciplinary survey.

Different diversity patterns of butterfly and moth assemblages between deciduous and evergreen broad-leaf forests

In this study, we compared the diversity and abundance of two groups of Lepidopteran insects—butterflies and moths—across deciduous (DBL) and evergreen broad-leaved (EBL) tree communities in warm temperate forests. Over three years (from 2018 to 2020) at the Wando Arboretum, we used transect and point methods to sample butterflies and light traps to sample moths. Our findings revealed that the total estimated species richness was higher in EBL compared to DBL. Non-metric multidimensional scaling (NMDS) analysis confirmed distinct moth assemblages between the two tree communities, while butterfly assemblages did not exhibit such separation. The seasonal diversity patterns of butterflies in both forests differed significantly, whereas those of moths exhibited a similar trend with a peak in June. Beta diversity components indicated high turnover rates in moth assemblages across both tree communities and high nestedness in butterfly assemblages within the DBL. Although the diet breadth of butterflies and moths did not significantly differ between the two tree communities, the number of butterfly species per host plant was higher in DBL and that of moths was higher in EBL. Considering the ongoing vegetation transition from DBL to EBL, changes in forest structure—such as canopy openness and tree density—may adversely impact insect communities within DBL.

Melatonin Delays Arthritis Inflammation and Reduces Cartilage Matrix Degradation through the SIRT1-Mediated NF-κB/Nrf2/TGF-β/BMPs Pathway.

Cartilage, a flexible and smooth connective tissue that envelops the surfaces of synovial joints, relies on chondrocytes for extracellular matrix (ECM) production and the maintenance of its structural and functional integrity. Melatonin (MT), renowned for its anti-inflammatory and antioxidant properties, holds the potential to modulate cartilage regeneration and degradation. Therefore, the present study was devoted to elucidating the mechanism of MT on chondrocytes. The in vivo experiment consisted of three groups: Sham (only the skin tissue was incised), Model (using the anterior cruciate ligament transection (ACLT) method), and MT (30 mg/kg), with sample extraction following 12 weeks of administration. Pathological alterations in articular cartilage, synovium, and subchondral bone were evaluated using Safranin O-fast green staining. Immunohistochemistry (ICH) analysis was employed to assess the expression of matrix degradation-related markers. The levels of serum cytokines were quantified via Enzyme-linked immunosorbent assay (ELISA) assays. In in vitro experiments, primary chondrocytes were divided into Control, Model, MT, negative control, and inhibitor groups. Western blotting (WB) and Quantitative RT-PCR (q-PCR) were used to detect Silent information regulator transcript-1 (SIRT1)/Nuclear factor kappa-B (NF-κB)/Nuclear factor erythroid-2-related factor 2 (Nrf2)/Transforming growth factor-beta (TGF-β)/Bone morphogenetic proteins (BMPs)-related indicators. Immunofluorescence (IF) analysis was employed to examine the status of type II collagen (COL2A1), SIRT1, phosphorylated NF-κB p65 (p-p65), and phosphorylated mothers against decapentaplegic homolog 2 (p-Smad2). In vivo results revealed that the MT group exhibited a relatively smooth cartilage surface, modest chondrocyte loss, mild synovial hyperplasia, and increased subchondral bone thickness. ICH results showed that MT downregulated the expression of components related to matrix degradation. ELISA results showed that MT reduced serum inflammatory cytokine levels. In vitro experiments confirmed that MT upregulated the expression of SIRT1/Nrf2/TGF-β/BMPs while inhibiting the NF-κB pathway and matrix degradation-related components. The introduction of the SIRT1 inhibitor Selisistat (EX527) reversed the effects of MT. Together, these findings suggest that MT has the potential to ameliorate inflammation, inhibit the release of matrix-degrading enzymes, and improve the cartilage condition. This study provides a new theoretical basis for understanding the role of MT in decelerating cartilage degradation and promoting chondrocyte repair in in vivo and in vitro cultured chondrocytes.

Environmental Health Impacts on The Sustainability of The Coral Reef Ecosystem in Gede Island, Indonesia

Gede Island, located north of Rembang Regency in Central Java, Indonesia, hosts coral reefs in poor environmental health. This study aimed to assess the impact of environmental health on various dimensions of sustainability to guide stakeholder decision-making. A descriptive quantitative method was employed. Observations of coral reef variables, including type and percentage of cover, were conducted using the Point Intercept Transect (PIT) method. The sustainability status of management practices was analysed using the Multidimensional Scaling (MDS) method with Rapfish (Rapid Assessment Techniques for Fisheries) 2013 version R software. The results identified 16 genera from three sampling points: Station 1 had 30.35% hard coral cover, Station 2 had 1%, and Station 3 had 12.94%. The sustainability status of Gede Island's coral reefs was evaluated across five attributes: ecological (41.61%), social (48.05%), economic (40.08%), institutional (26.36%), and technological (57.03%). Among these, only the technological attribute was classified as moderately sustainable, while the others were deemed less sustainable. A high sustainability status offers significant potential and benefits for the local community, such as opportunities for ecotourism and fisheries sustainability.

The Nrf2/HO-1 pathway participates in the antiapoptotic and anti-inflammatory effects of platelet-rich plasma in the treatment of osteoarthritis.

We aimed to explore the molecular mechanisms through which platelet-rich plasma (PRP) attenuates osteoarthritis (OA)-induced pain, apoptosis, and inflammation. An in vivo model of OA was established by injuring rats using the anterior cruciate ligament transection method, whereas an in vitro model was generated by exposing chondrocytes to interleukin (IL)-1β. Both models were then treated with PRP. In both the in vivo and in vitro models, OA led to the suppression of the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway, whereas treatment with PRP reactivated this molecular axis. Inhibition of the Nrf2/HO-1 pathway using the Nrf2 inhibitor brusatol or through Nrf2 gene silencing counteracted the effects of PRP in reducing the tenderness and thermal pain thresholds of OA rats. Additionally, PRP reduced the mRNA expression of IL-1β, IL-6, tumor necrosis factor-alpha (TNF-α), and matrix metallopeptidase 13 (MMP-13) and the protein expression of B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X-protein (Bax), and caspase-3. Furthermore, inflammation and apoptosis were induced by brusatol treatment or Nrf2 silencing. Additionally, in the in vitro model, PRP treatment increased the proliferation of chondrocytes and attenuated their inflammatory response and apoptosis, effects that were abrogated by Nrf2 depletion. The Nrf2/HO-1 pathway participates in the PRP-mediated attenuation of OA development by suppressing inflammation and apoptosis.

The distribution of macroalgae in the Aceh north-western coastline

Abstract Macroalgae are polyphyletic multicellular plants that generally inhabit the littoral zone of the seashore. Aceh Province has a coastline stretching along the east, north and west ends of Sumatra Island; thus, various macroalga species may be found. This research aims to study the distribution of macroalga species at four locations on the north-western side of Aceh, including three sites in Aceh Besar District (Lhoknga, Lange, and Ujung Pancu Beaches) and one site in Banda Aceh City (Ulee Lheue Beach). Sampling was done across 50 meters of the seashore and 1 meter towards the sea. The sampling was conducted using a 1×1 meter quadrant transect method with sampling points every 10 meters. The morphology determination shows that five species of macroalga grew at Lhoknga Beach, namely Valoniopsis pachynema, Halimeda opuntia, Turbinaria decurrens, Sargassum plagiophyllum, and Padina australis. Lange Beach had three macroalgae species: Chaetomorpha linum, Laurencia obtusa, and Sargassum plagiophyllum. Meanwhile, Ujung Pancu Beach has four macroalga species: Halymenia durvillei, Turbinaria decurrens, Ulva lactuca and Sargassum plagiophyllum. At Ulee Lheue Beach, five species were found: Gracilaria edulis, Caulerpa taxifolia, Hypnea musciformis, Padina australis, and Acanthophora spicifera. In total, 13 macroalga species were distributed on Aceh’s north-western coast.

Abundance of Marine Debris on Satonda Island, West Nusa Tenggara, Indonesia

Abstract Macro-sized marine debris is found not only on the coasts of densely populated islands but also on the coasts of small islands. The presence of marine debris on this small island is because there are tourist attractions that attract people’s attention or because currents around it carry it away. Therefore, this research aims to determine the abundance of marine debris on Satonda Island (a small island in the eastern part of Sumbawa Island, West Nusa Tenggara Province, Indonesia). Even though it is small, this island is an attractive tourist destination because it has a lake formed from the eruption of Mount Tambora. Marine debris data was collected using the quadrant transect method, carried out randomly along the coastline. Data collection time was carried out at high and low tides, with the parameters recorded being the amount and type of waste, size and shape of rubbish, coordinate points, transect length, and data collection time. The research results show that the marine debris on Satonda Island is diverse (393 items), with a total weight of 16,665.45 g. At high tide, it is dominated by rubber marine debris, such as shoes and sandals weighing 1,457 g (33 items), while at low tide, it is dominated by hard plastic marine debris weighing 3,972 g (147 items).

Initial Assessment of the Benthic Profile and Reef Fish Composition of the Damilisan Marine Sanctuary, Miagao, Iloilo, Philippines

Coral reefs are among the world’s most diverse and productive ecosystems. They provide valuable ecosystem services, including support for fisheries production and the livelihood of coastal communities. However, coral reefs are also threatened by anthropogenic and climate stressors. Establishing a marine protected area (MPA) is a typical coastal management tool widely used in the Philippines for conserving coral reefs and managing the fishing effort. This study presents initial information on the benthic and fish community profiles of the Damilisan Marine Sanctuary (DMS) in Miagao, Iloilo, Philippines. A modified point-intercept transect method was used to characterize the benthic profile, while the underwater visual census (UVC) was used to determine the fish community profile (diversity and density). The substrate was mainly composed of hard coral (HC) (39.3±18.3%), and the common HC observed were massive Porites and Goniopora, each contributing 12.8% of the total HC cover. Twenty coral reef fish species belonging to 10 families were identified during the UVC and had an overall density of 99±69 ind./1000 m2. Damselfishes (Family Pomacentridae) was the most abundant family (23±0.4 ind./500 m2), while Lutjanus semicinctus was the most abundant species (8.5±1.4 ind./500 m2). It is recommended that regular coral reef monitoring surveys be conducted in the DMS and in other MPAs in Miagao to monitor trends and changes in the HC cover and reef fish density and diversity in the MPA.

Vascularity evaluation of the equine tarsocrural and proximal intertarsal joint septum, and comparative analysis of two arthroscopic transection techniques.

To evaluate vascularity of the synovial membrane covered septum (SMS) separating the tarsocrural (TC) and proximal intertarsal (PIT) joints (Part 1) and compare two methods of transection, electrosurgical or Ferris Smith rongeur (FS rongeur) (Part 2). Experimental study. Part 1, 10 SMS (n = 5 horses). Part 2, six horses (n = 12 tarsi). In part 1, SMS harvested postmortem were each divided into eight regions of interest (ROIs), processed for histology, and immunostained with anti-α-actin antibody for blood vessel identification. Vascular density was calculated for each ROI. Data was compared within and between horses. In part 2, six horses underwent TC arthroscopy. Each limb was randomly assigned to undergo either electrosurgical or FS rongeur SMS transection. SMS transection and total operative time were recorded. Intraoperative hemorrhage was scored. Data was compared between both techniques. Significant interindividual variations in SMS vascular density were detected (p = .02), but there were no differences among ROIs. No differences in the transection time were detected between electrosurgery (4.83 ± 0.54 min) and FS rongeur (4.33 ± 0.67 min). No differences were found in intraoperative hemorrhage scores between techniques. Vascularity within the SMS varies among horses but not within its regions. Electrosurgical or FS rongeur transection of the medial SMS during tarsocrural arthroscopy is a rapid technique and improves surgical access to the dorsal compartment of the PIT.

Habitat Characteristics and Morphology of Chlorophyta (Order Bryopsidales) on the Intertidal of Pandanan Hamlet, West Sekotong

Bryopsidales order macroalgae can be found on various substrates in marine waters. The Bryopsidales order has a morphological form that is shaped like a filament or shaped like a leaf and has pigments like land plants. The study aims to determine the habitat characteristics and morphology of the Bryopsidales order on the coast of Pandanan Hamlet. Sampling was carried out using the line transect method and 1x1 meter quadrant. A total of 5 transect lines were stretched from the shoreline to the point where macroalgae were found. Each transect is placed in quadrants every 20 meters in a zigzag manner. The Bryopsidales order found consisted of 13 species, 3 families and 4 genera growing in habitats with substrates of loamy sand, loam, sand, and sandy loam, rock, dead coral, coral fragments, and live coral. There are 3 types of holdfast were found, namely bulbous, fibrous, and sprawling growers. There are also three blade shapes in this order, namely creeping blades in the Caulerpaceae family, segmented blades in the Halimedaceae family, and blades that are neither creeping nor segmented that resemble sponges in the Codiaceae family.

Current status and future perspectives of robotic liver surgery.

Robotic liver resection has been reported in case series since the early 2000s. The surgical robot is capable of precise operation using articulated forceps with seven degrees of freedom. The robot also eliminates tremors and provides a good surgical field with highly detailed 3D high-definition images. The clinical results demonstrating their usefulness have been increasing year by year. Initially, a shorter hospital stay was observed in comparison with open hepatectomy. Recent reports have also shown lower conversion and complication rates in comparison with laparoscopic hepatectomy. The clamp and crush method with bipolar forceps, sealing devices, ultrasonic shears, and the combined procedure of Cavitron ultrasonic surgical aspirator and robotic forceps as hybrid procedures have been reported as effective methods of parenchymal transection in robotic surgery. Theoretically, the advantages of the robotic platform allow for more complex liver resection around hilar structures and major blood vessels, as well as for vascular reconstruction or biliary reconstruction. The application of robotic liver surgery for hilar cholangiocarcinoma, living donor hepatectomy, and living donor liver transplantation has been reported. Robotic liver surgery is becoming more popular for certain indications; however, it is important to further evaluate its long-term surgical and oncological outcomes and costs in comparison with conventional laparoscopic and open liver surgery, based on accumulated experience.

Analysis of the safety and efficacy of the self-pulling and latter transected technique in modified overlap anastomosis in total laparoscopic total gastrectomy

BackgroundLaparoscopic total gastrectomy plus lymph node dissection is an effective treatment method for patients with gastric cancer. With the development and popularization of laparoscopic techniques in recent years, surgeons have become more skilled in laparoscopic techniques. Totally laparoscopic total gastrectomy (TLTG) has been developed; however, digestive tract reconstruction remains difficult, especially with anastomosis of the esophagus and jejunum. Using the self-pulling and latter transection (SPLT) method combined with a linear stapler has effectively solved the problem of narrow space in esophagojejunostomy. Here, we examined the safety and effectiveness of the SPLT technique in TLTG compared with SPLT with traditional esophagojejunostomy overlap anastomosis.MethodsWe retrospectively analyzed all patients with gastric cancer admitted to the Department of Gastrointestinal Surgery of the Second Affiliated Hospital of Fujian Medical University from September 2020 to September 2023. In total, 158 patients met the inclusion criteria and were included. Patients were grouped according to whether the lower esophagus was transected after self-pulling. Patient demographics, tumor characteristics, surgical conditions, and postoperative results between the two groups were statistically analyzed.ResultsA total of 158 patients were included in the study. All patients underwent TLTG and completed intracavitary anastomosis. There were 70 cases (44%) in the SPLT-Overlap group and 88 cases (56%) in the traditional overlap group. There was no significant difference in demographic and oncological characteristics between the two groups. The operation time (P = 0.002) and esophageal jejunum anastomosis time (P<0.001) were significantly shorter in the SPLT-Overlap group compared with the traditional overlap group. The intraoperative blood loss of the SPLT-Overlap group was 80.29 ± 36.36 ml, and the intraoperative blood loss of the traditional overlap group was 101.40 ± 46.68 ml. The difference was statistically significant (P=0.003). The SPLT-Overlap group also achieved a higher upper cutting edge (P =0.03). There was no significant difference between the two groups in terms of the incision size, postoperative hospital stay, time to first flatus, time to first liquid intake, drainage tube removal time, and esophagojejunal anastomotic diameter. There were 15 and 19 cases of short-term postoperative complications in the SPLT-Overlap and traditional Overlap groups, respectively. All patients received R0 resection, and no secondary surgery or death occurred.ConclusionWe applied SPLT to overlap anastomosis. Short-term, SPLT has good safety and feasibility in TLTG. It can effectively shorten the time of digestive tract reconstruction, simplify the reconstruction procedure, and make the digestive tract reconstruction simple and fast; at the same time, a safe cutting edge can be obtained.

Avifaunal Diversity of Cantonment Area, Lucknow, Uttar Pradesh, India

Birds have always fascinated mankind with their naturally attractive plumage, harmonious songs and creative behavior. Avifaunal Diversity is one of the most important ecological indicators, displays the environmental quality of an area, its pollution level, safety and accessibility of food and habitat. Cantonment area is one of the distinctive locations represents a sub-urban area of Lucknow. Cantonment area is an abundant green area around 23.43 square kilometer, preferred by number of bird species at its different types of habitat like shaded trees, bushes, grasses, grounds some parks and buildings etc. They provide food and shelter for the urban terrestrial bird’s community. The study was performed in Cantonment area for six months from February, 2022 to July, 2022. Bird surveys were carried out using the Line transect method across four different transects at this area. As a result of six months observation, 55 bird species were recorded belonging to 15 orders and 29 families. Majority of species were observed in the order Passeriformes and family Muscicapidae. Data analysis showed that 29 avian species were Common, 12 were Fairly Common, 6 were Uncommon and 8 were Rare, whereas 52 avian species were residents and 3 avian species were migrants.

EDNA Metabarcoding Reveals the Species-Area Relationship of Amphibians on the Zhoushan Archipelago.

The species-area relationship is important for understanding species diversity patterns at spatial scales, but few studies have examined the relationship using environmental DNA (eDNA) techniques. We investigated amphibian diversity on 21 islands of the Zhoushan Archipelago and nearby mainland areas in China using the combination of eDNA metabarcoding and the traditional line transect method (TLTM) and identified the species-area relationship for amphibians on the islands. The mean detection probability of eDNA is 0.54, while the mean detection probability of TLTM is 0.24. The eDNA metabarcoding detected eight amphibian species on the islands and nine species in the mainland areas, compared with seven species on the islands and nine species in the mainland areas that were identified by TLTM. Amphibian richness on the islands increased with island area and habitat diversity. The species-area relationship for amphibians in the archipelago was formulated as the power function (S = 0.47A0.21) or exponential function (S = 2.59 + 2.41 (logA)). Our results suggested that eDNA metabarcoding is more sensitive for the detection of amphibian species. The combined use of eDNA metabarcoding and the traditional line transect method may optimize the survey results for amphibians.

The Potential of Coral Reef as Support of Marine Ectourisme at Sidodadi Village, Pesawaran Regency, Province of Lampung

Coral reefs can be used for marine ecotourism by considering suitability and carrying capacity analysis. The aim of this research is to analyze the potential of coral as a support for marine ecotourism in Sidodadi Village, Teluk Pandan District, Pesawaran Regency, Province of Lampung. Data collection on the percentage of cover and type of growth (life form) of coral was carried out using the Underwater Photo Transect (UPT) method, while coral fish used the Underwater Visual Census (UVC) method. The results of the research show that the physico-chemical parameter conditions of the waters are suitable for marine ecotourism, coral cover ranges from 0,12-78,03%, 523 reef fish consist of 7 major fish families, 5 target fish families and 1 indicator fish family, suitability tourism at each station in the research location where station 1 is 59,25% (Conditionally Appropriate), station 2 is less than 40,74% (Not Appropriate), station 3 is 88,88% (Suitable) and station 4 is 59,25% (Conditionally Appropriate). The area's carrying capacity is 106 people/day and the utilization carrying capacity is 11 people/day.

Heterogeneric Diversity and Richness of Avifauna in a Degraded Landscape of Arial Beel in Bangladesh

Bird diversity and richness reflect the habitat quality of ecosystems. The species composition, diversity, and richness of birds were studied at Shologhar Union, a degraded landscape of Arial Beel, from March 2016 to February 2017, using line transect and point count methods. A total of 75 species of birds belonging to 14 orders and 35 families were recorded; most of them were residents (83%). Non-passerine birds were predominant (40 species, 53% of total species), with 18 families and 38 genera. Only one near-threatened species (Yellow Wattled Lapwing Vanellus malabaricus) was recorded. Among resident birds, Baya weaver (Ploceus philippinus) was dominant, and the Yellow Wagtail (Motacilla flava) was predominant among migratory birds. Most species were terrestrial (51 spp., 68% of total birds), contributing only 23% of the population. The individual rarefaction revealed the highest species richness in March and the lowest in May and June. The highest population was recorded in winter (38%), and insectivore species (40%) were the highest. No significant seasonal variation occurred in the richness of birds (Kruskal-Wallis test: K = 0.05 df = 2, p = 0.971) and in different feeding guilds (Kruskal-Wallis test: K = 0.86, df = 5, p = 0.65). Ardeidae and Sturnidae represented the highest diversified families (6 species in each, RDi = 8), and Baya Weaver contributed the highest (26%) population. The presence of different species of birds suggests that this site could provide a suitable habitat for wild birds, and restoration management of this part of Arial Beel should require conserving the habitat of avifauna. Jagannath University Journal of Life and Earth Sciences, 9(1): 19-44, 2023 (June)

Carbon stock, density, and biomass of seagrass in South Bangka, Indonesia

Abstract. Supratman O, Farhaby AM, Henri, Septiyati MU, Adi W. 2024. Carbon stock, density, and biomass of seagrass in South Bangka, Indonesia. Biodiversitas 25: 1736-1742. As a component of the carbon ecosystem, seagrass meadows play a crucial role in preventing climatic change. The execution of Indonesian programs pertaining to carbon economic value, carbon trading, and other endeavors aimed at attaining the Nationally Determined Contribution (NDC) requires estimated data on seagrass carbon stocks. The present study investigates the seagrass density, vegetation carbon stock, and sediment carbon stock of South Bangka, Indonesia. The investigation is carried out in the following stages: i) data collection on-site, ii) laboratory sample analysis, and iii) data analysis. The accumulation of data on-site utilizes a square transect method, which includes the gathering of sediment, sample biomass, and density information. The carbon composition of sediment and seagrass vegetation is ascertained through laboratory analysis employing the Loss of Ignition (LOI) method. The investigation uncovered eight species of seagrass in South Bangka. Cymondocea rotundata, Enhalus acoroides, Halophila ovalis, Halodule pinifolia, Halodule uninervis, Oceana serrulata, Siringodium isotifolium, and Thalassia Hemprichii are the identified species of seagrass. In South Bangka, the combined carbon stock of seagrass (above and below ground carbon) varies between 0.049±0.057 and 1.323±1.581 Mg C Ha-1. The range of sediment carbon is 110.53±42.07 Mg Corg Ha-1 extrapolated to a depth of 1 m. The results of this study suggest that the seagrass meadows in South Bangka are characterized by a significant species diversity and effectively sequester carbon in the form of biomass and sediment. This supports the National Climate Change Mitigation Program for Blue Carbon Ecosystems, an initiative that seeks to mitigate the adverse effects of climate change by utilizing the carbon sequestration capabilities of mangrove forests, seagrass meadows, and tidal marshes.

Butterflies (Lepidoptera: Papilionoidea) diversity in Kedung Klurak Tourism Area, Mojokerto District, East Java, Indonesia

Abstract. Umami S, Amaliyah NS, Fahlefi AR, Zumar MR, Romzalis AA, Wibisana OR, Susanto MAD. 2024. Butterflies (Lepidoptera: Papilionoidea) diversity in Kedung Klurak Tourist Area, Mojokerto District, East Java, Indonesia. Intl J Trop Drylands 8: 27-34. Kedung Klurak is a natural tourism area with very good environmental conditions and is naturally maintained. This study aims to identify and analyze the diversity of butterflies (Lepidoptera: Papilionoidea) in the Kedung Klurak Tourism Area, Mojokerto District, East Java, Indonesia. The sampling method used in this study was the sweeping net and cruising or transect method. The research location was divided into three locations: the riverbank, pine forest, and bamboo area. The results of this study found 37 species, with a total of 593 individuals belonging to 5 families. The index value of species diversity in the Kedung Klurak Tourism Area is H = 3.313 in the high category. The highest diversity index is at the riverbank location with H = 3.313, followed by pine forest with H = 3.060, and the lowest diversity index is at the bamboo area location with H = 2.811. Several factors affecting differences in butterfly species diversity (Lepidoptera) are their location because they have different environmental conditions and are influenced by the availability of biotic and abiotic factors, food, and host plants. Abiotic factors include light intensity, temperature, and air humidity.

Assessment of welfare of Pekin ducks reared in intensive production system in Poland using transect method

Abstract The aim of this study was to evaluate the practical application of the Transect Walk (TW) method on a commercial farm, focusing on different ages of Orvia ST5 Pekin ducks. The study was conducted in four flocks of Orvia ST5 ducks, reared in two identical buildings. Flocks 1 and 3 were housed in Building 1, while flocks 2 and 4 occupied Building 2. A total of 8,000 ducklings were allocated to rearing, with 2,000 in each flock. The welfare of the ducks was assessed at 5, 6, and 7 weeks of age using the TW method, which involved assessing indicators such as immobility, lameness, dirtiness, small size, head wounds, back wounds, tail wounds, feather loss, illness, terminal illness, being flipped, or being deceased. Information regarding factors contributing to reduced carcass quality was gathered from the slaughterhouse. It was observed that all welfare indicators, except for head and tail wounds, were equally effective in assessing duck welfare and accounted for up to 82.13% of the variation in welfare. Lameness emerged as the most prevalent factor associated with reduced welfare, showing a strong correlation with immobility. Both indicators exhibited a significant interaction with age and flock (P = 0.001 and P = 0.029, respectively). Feather loss emerged as another crucial indicator of reduced welfare in the examined flocks. The percentage of featherless ducks varied from 0.29% to 3.50% and was significantly influenced by both duck age and flock (both P<0.001). Additionally, it showed a significant and positive correlation with immobility (P<0.05). The primary issues identified during duck culling were associated with skin problems and leg and joint issues. Statistically significant variations were noted among flocks concerning the frequency of indicators linked to reduced welfare. This variance was attributed to the specific building where the ducks were raised. The findings of this study suggest the incorporation of the TW method in future welfare assessments, utilizing the 10 welfare indicators employed while excluding head and tail wounds.