Genetic variability is the basic requirement for improving crops which further leads to progress in crop breeding. This study was carried out to determine the inheritance pattern of various agronomically important traits in F2 populations of mustard genotypes. The objective of this study was to determine the genetic variability for agro-morphological traits under control conditions (CC) and heat stress environments (HS) and provide the future strategies to improve this important oilseed crop. The experiment was conducted at the Botanical Garden, Sindh Agriculture University, Tandojam. During 2022-23, a total of 27 genotypes including nine parental mustard genotypes and eighteen F2 progenies were grown under control and heat stress conditions. The experiment was laid out in a randomized complete block design with three replications. The analysis of variance for the genotypes, treatments, and F2 progenies revealed significant differences (P≤0.05) in most of the studied characters, highlighting the importance of genetic variations in the evaluated breeding materials of mustard for stress breeding. When estimating heritability for different characters, including seed yield plant-1, it was found that F2 crosses showed varying levels of heritability for different characteristics, ranging from low to moderate and high. This implies that careful selection may be conducted for traits that have low heritability, especially for seed yield.; however, those characters which showed high heritability would be selected at early generation stage to improve the characters.