Advanced Driver Assistance System (ADAS) represents a collection of vehicle-based intelligent safety systems. One in particular, Traffic Sign Recognition System (TSRS), is designed to detect and interpret roadside information in the form of signage. Even though TSRS has been on the market for more than a decade now, the available ones differ in hardware and software solutions they use, as well as in quantity and typology of signs they recognize. The aim of this study is to determine whether differences between detection and readability accuracy of market-ready TSRS exist and to what extent, as well as how different levels of “graphical changes” on the signs affect their accuracy. For this purpose, signs (“speed limit” and “prohibition of overtaking”) were placed on a test field and 17 vehicles from 14 different car brands underwent testing. Overall, the results showed that sign detection and readability by TSRS differ between car brands and that even small changes in the design of signs can drastically affect TSRS accuracy. Even in a controlled environment where no sign has been altered, there has been a 5% margin of misread signs.