Time-dependent origin–destination (OD) matrices are essential input for dynamic traffic models such as microscopic and mesoscopic traffic simulators. Dynamic traffic models also support real-time traffic management decisions, and they are traditionally used in the design and evaluation of advanced traffic traffic management and information systems (ATMS/ATIS). Time-dependent OD estimations are typically based either on Kalman filtering or on bilevel mathematical programming, which can be considered in most cases as ad hoc heuristics. The advent of the new information and communication technologies (ICT) provides new types of traffic data with higher quality and accuracy, which in turn allows new modeling hypotheses that lead to more computationally efficient algorithms. This article presents ad hoc, Kalman filtering procedures that explicitly exploit Bluetooth sensor traffic data, and it reports the numerical results from computational experiments performed at a network test site.