Highways with low traffic volume pose a particular kind of safety risk. Among the various causes of traffic accidents in China, driving behavior is important and cannot be neglected. In this research, the Golumd–Kunlun Mountain section of the low-volume Qinghai–Tibet highway was chosen for real-vehicle testing with a random sample of 25 drivers. A dynamic Global Positioning System device, a KF2 dynamic multiparameter physiology and psychology recorder, and an SMI iViewX HED system were employed for real-time measurements of vehicle speed, location, fixation, driver workload, and other parameters. With consideration of the dynamic interactions between driver, vehicle, road, and environment, the relationship between traffic safety and driving behavior was analyzed, as well as eye movement and driving marks on the selected highway. Analysis of the characteristics of traffic accidents in the test section showed that driving behavior was the major cause of traffic accidents. The conclusions provide important insights for traffic safety technology and management on low-volume roads in the plateau area.