Traditional Plant Research Articles

Cover crop alters rhizosphere sediments to recruit plant growth-promoting microorganisms, enhancing peanut production

Peanuts are typically grown in continuous monoculture in China, leading to continuous cropping obstacles and reduced yields. Cover cropping is emerging as a pivotal strategy for enhancing soil quality and promoting sustainable agriculture. However, the effects of cover crops on the rhizosphere environment, and how they benefit subsequent crops, are not well understood. We hypothesize that planting cover crop ryegrass during the winter fallow period could alter peanut rhizosphere sediments, recruit plant growth-promoting microorganisms, and ultimately enhance peanut production. This study aims to fill this gap by investigating the impact of ryegrass cover cropping on rhizosphere sediments and peanut yield. Rhizosphere soil organic carbon, total nitrogen, pH, and soil enzymes, including sucrase, urease, N-acetyl-β-D-glucosaminidase, and β-glucosidase, were analyzed, along with metabolomics, 16S rRNA, and fungal ITS sequence analyses, under traditional planting management (TP) and long-term cover crop (CC) treatments. The results showed that CC treatment significantly increased rhizosphere pH, enhanced soil organic carbon and total nitrogen levels, and elevated soil enzyme activities compared to TP treatment. Metabolomic analysis revealed that CC treatment upregulated compounds such as D-pinitol, palmitoleic acid, fructose, sorbitol, and sucrose, while downregulated compounds including benzoic acid, dodecanoic acid, and carbamic acid. This alteration in metabolite composition influenced the recruitment and function of the microbial community. Specifically, CC treatment markedly enhanced the abundance of growth-promoting microbes such as Bacillus, Paenibacillus, Pseudomonas, Lysobacter, Bradyrhizobium, and Mortierell etc., which are involved in important functions such as chemoheterotrophy, nitrate reduction, and plant saprotroph. Simultaneously, there was a decrease in pathogenic microorganisms, such as Aspergillus flavus and Fusarium oxysporum. Moreover, CC treatment positively influenced peanut root growth, resulting in longer roots and ultimately increase pod yield by 21.15 % compared to traditional winter fallow practices. These findings underscore the potential of cover cropping with ryegrass to improve rhizosphere microecosystem components, and ultimately enhance peanut productivity, providing valuable insights for sustainable agricultural practices.

Nutritional Properties of Wild Edible Plants with Traditional Use in the Catalan Linguistic Area: A First Step for Their Relevance in Food Security.

Wild food plants (WFPs) are crucial for the subsistence of many human populations. While there are at least 7000 edible plant species in the world, only approximately 420 are considered food crops. WFPs are often studied from the phytochemical and pharmacological point of view, because they include available food components with nutraceutical value. The present study aims to highlight the nutritional value of WFPs traditionally used in the Catalan linguistic area, providing detailed insights and discussing the significance of these properties. Information about the nutritional properties of 93 taxa, coming from ethnobotanical prospection, has been collected through an extensive bibliographic research. The results reveal that WFPs are rich in nutrients, especially micronutrients. Furthermore, in selected species, those for which nutritional information and a cultivated homologue are available, the nutrient content in wild taxa exceeds than of phylogenetically related crop plants with similar use. Traditional wild plant preparation forms for food and the nutritional value of a menu constituted by wild food plants are presented. This research represents a preliminary step toward selecting certain taxa that could be developed into new small- or large-scale crops or sustainably harvested in the wild, contributing to food security.

Bleomycin-Induced Fibrosis and the Effectiveness of Centella Asiatica as a Treatment.

Plant treatment has been used for thousands of years and has been proven to treat acute and chronic diseases. The function of the traditional plant Centella asiatica is as an antimicrobial agent, anticancer, antioxidant, and therapeutic gene in healing wounds and inflammation. Lung fibrosis caused by bleomycin can develop into chronic lung disease, which ends in tissue death if not treated immediately. The purpose of this study is to predict and explain the impact of Centella asiatica extract on model rats exposed to bleomycin in their lungs as a treatment or anti-fibrinolysis. This research is an analytical study with a randomized in-vivo experimental design divided into 3 groups of 5 male Wistar rats aged 10 weeks. Negative control group (K) with intratracheal induction of bleomycin alone. The positive group was given intratracheal bleomycin 4 mg/kg/BB on days 0 and 21 and added Centella asiatica induction at 400 mg (P1) on days 15 to 49. The other positive group was given intratracheal bleomycin 4 mg/kg/BB on days 0 and 21 and added Centella asiatica induction at 800 mg (P2) on days 15 to 49. Data were collected according to findings of lung histology analysis of rat samples. In the interalveolar septum group, there was a difference in Masson's Trichrome staining results in groups K and P1 with p<0.05 (p=0.036). However, there was no difference in histopathological staining results in groups K and P2 (p>0.05). The induction of bleomycin 4 mg/kg/BB was proven to cause fibrosis in the lungs of rats, and Centella asiatica extract was used as a treatment. Therefore, further research regarding antifibrotic drugs is hoped to reduce fibrotic areas significantly.

Phytochemical Identification of Alkaloidic Compounds from Cinnamomum cassia Cortex and Estimation of their Antifungal Efficacy

Cinnamomum caresia is defined as a medical and traditional plant grows in diverse countries in the world. It has therapeutic effects for treating various diseases resulting from infection by different microorganisms. In the current study, six alkaloids compounds were isolated and characterized from cinamom cortexes by using the GC-MS technique. The synergistic interaction was applied for the alkaloidic mixture against growth of two isolates of pathogenic fungi represented by Urocystis agropyri and Candida albicans by using the concentrations (25, 50, 75, 100, and 125 mg/ml) which recorded diverse diameters of inhibition equal to 58, 66, 72, 74, and 78 mm and 68, 52, 70, 72, and 74 mm, respectively. As a result, these alkaloidic compounds are recommended for use as natural therapy for diseases caused by the current pathogenic fungi.

The Influence of Cichorium Endivia and Urtica Dioica Extracts Against H2O2-Induced Stress in SH-SY5Y Cells

Aim: In neurodegenerative diseases, increased production of hydrogen peroxide (H2O2) causes oxidative stress in neurons, and when the damage is severe, it is irreversible and apoptosis is induced. In recent years, phytochemicals that exhibit neuroprotective and antioxidant properties to protect neurons from oxidative stress have gained importance. Therefore, herbal extracts with high antioxidant capacity and anti-apoptotic properties may provide a protective strategy against H2O2-induction. This study aims to examine the influence of Cichorium endivia liquid extract (CEE) and Urtica dioica liquid extract (UDE) against H2O2-induction on SH-SY5Y cells in vitro. Method: For this purpose, cells were treated with the extracts and then exposed to H2O2. The neuroprotective effect and cell viability of these extracts were evaluated by XTT method. Antioxidant activity was determined by the CUPRAC method. Finally, the apoptotic cell death and reactive oxygen species (ROS) levels were examined with DAPI and DCFH-DA, respectively. Results: The results obtained showed that CEE and UDE have an influence on neuroprotection, anti-apoptotic, and antioxidant effects by reducing H2O2-induced toxicity, apoptotic cell death, and ROS levels. Conclusion: H2O2 causes neurotoxicity in neurons through oxidative damage. To prevent this, traditional plant extracts with high antioxidant activity can provide a neuroprotective strategy. As a consequence, it was shown in an in vitro model that these herbal extracts could be a neuroprotective effect.

Yeast Proteins: Proteomics, Extraction, Modification, Functional Characterization, and Structure: A Review.

Proteins are essential for human tissues and organs, and they require adequate intake for normal physiological functions. With a growing global population, protein demand rises annually. Traditional animal and plant protein sources rely heavily on land and water, making it difficult to meet the increasing demand. The high protein content of yeast and the complete range of amino acids in yeast proteins make it a high-quality source of supplemental protein. Screening of high-protein yeast strains using proteomics is essential to increase the value of yeast protein resources and to promote the yeast protein industry. However, current yeast extraction methods are mainly alkaline solubilization and acid precipitation; therefore, it is necessary to develop more efficient and environmentally friendly techniques. In addition, the functional properties of yeast proteins limit their application in the food industry. To improve these properties, methods must be selected to modify the secondary and tertiary structures of yeast proteins. This paper explores how proteomic analysis can be used to identify nutrient-rich yeast strains, compares the process of preparing yeast proteins, and investigates how modification methods affect the function and structure of yeast proteins. It provides a theoretical basis for solving the problem of inadequate protein intake in China and explores future prospects.

Buckwheat: An Underutilized Himalayan Crop with Multifaceted Nutraceutical Benefits.

The human population is growing and alternate food options are needed to provide food and nutritional security to mankind. Reduced agricultural output as a result of climate change and increased demand for grains because of continuous population growth have created a gap between demand and supply of food. Buckwheat is a pseudocereal crop plant with high nutritional value that can be included as an alternate food in our diet. It is a traditional crop plant grown in the high mountains of the Himalayas for food as well as fodder. It completes its life cycle in 3-4 months, so is mostly grown as a second crop in between main crops like maize and barley. It also acts as a green manure by improving the phosphorus content of the soil. Buck-wheat has high nutritional value as it is rich in essential amino acids, vitamin B, trace elements, and other nutrients. The main bioactive compounds identified in buckwheat are rutin, quercetin, isoquercetin, d-chiroinositol, resveratol, and vitexin, which are responsible for its pharmacolog-ical properties. Research focused on value addition by exploring its nutritional, pharmaceutical, and other alternative uses of commercial importance, is needed for reviving buckwheat cultiva-tion practices and its conservation. Considering the multifarious applications of buckwheat, this review summarizes the currently available knowledge on the agronomic and nutraceutical sig-nificance of buckwheat to project its value as a future crop in the avenue of agriculture and functional food.

Trends in Greenhouse Production Technology with Special Reference to Protected Cultivation of Horticultural Crops

Greenhouse is an important application scenario of modern agriculture. With the improvement of people's life quality, the demand for vegetables, fruits, flowers and has also increased, and the amount of greenhouses has developed rapidly. Greenhouse adopts artificial technical means to change natural light and temperature conditions, and to create environmental factors to optimize the growth of animals and plants, so that greenhouse crops can grow throughout the year and the weather. Compared with traditional planting methods, the greenhouse is more automated, intelligent, and mechanized. Greenhouse farming (Protected cultivation) refers to the practice of growing crops in an enclosed or partially enclosed environment, such as greenhouses, shade houses, or high tunnels. The primary purpose of protected cultivation is to create a controlled microclimate that shields the crops from adverse weather conditions, pests, and diseases, while optimizing environmental factors. It involves the use of structures, materials, and technologies that provide a protective barrier and allow for manipulation of temperature, humidity, light, and other environmental variables. The benefits of greenhouses are high production per unit area because the genetic potential of the crop can be completely exploited, vegetables and nurseries can be grown off-season that obtain high market prices, good quality products and it is easy to protect the crops from pests, diseases and extreme climatic conditions. Greenhouse farming is a highly intensive industry that needs considerable labour and capital inputs. Because of this, all factors important for a successful enterprisers should be carefully considered by potential growers.

Predicting Cd accumulation in crops and identifying nonlinear effects of multiple environmental factors based on machine learning models

The traditional prediction of the Cd content in grains (Cdg) of crops primarily relies on the multiple linear regression models based on soil Cd content (Cds) and pH, neglecting inter-factorial interactions and nonlinear causal links between external environmental factors and Cdg. In this study, a comprehensive index system of multi-type environmental factors including soil properties, geology, climate, and anthropogenic activity was constructed. The machine learning models of the tree-based ensemble, support vector regression, artificial neural network for predicting Cdg of rice and wheat based on the environmental factor indexes significantly improved the accuracy than the traditional models of linear regression based on soil properties. Among them, the tree-based ensemble models of XGboost and random forest exhibited highest accuracies for predicting Cdg of rice and wheat, with R2 in the test dataset of 0.349 and 0.546, respectively. This study found that soil properties, including Cds, pH, and clay, have greater impacts on Cdg of rice and wheat, with combined contribution rates accounting for 65.2 % and 29.7 % respectively. Since wheat sampling areas are located in central and northern China, they are more constrained by precipitation and temperature than rice sampling areas in the south. Geologic and climate factors have a greater impact on Cdg of wheat, with a combined contribution rate of 49.9 %, which is higher than the corresponding rate of 20.9 % in rice. Furthermore, the Cdg of rice and wheat did not exhibit an absolute linear relationship with Cds, and excessively high Cds can reduce the bioconcentration factor of Cd accumulation in crops. Meanwhile, other environmental factors such as temperature, precipitation, elevation have marginal effects on the increase of Cdg of crops. This study provides a novel framework to optimize traditional soil plant transfer models, as well as offer a step towards realizing high precision prediction of Cd content in crops.

Molecular Marker Techniques and Genotypic Characterization Approaches in Plant Breeding

Plant breeding, often known as the science of plant development, is the study and practice of modifying a plant’s genetic makeup through a variety of breeding techniques to produce higher-quality, more prolific, and more resistant to harsh environmental circumstances. Classical breeding programs are indispensable techniques for increasing yields and improving plant characteristics, but they are progressing too slowly to meet the increasing food demand of the rapidly growing world population alone. Considering that the development periods of plants are generally long in traditional plant breeding, the opportunity to develop higher quality and more productive species that are more resistant to abiotic and biotic stress factors is very limited. Because there are multiple steps required in producing new plant varieties, including hybridization, selection, and testing, the process of creating a new variation takes several years. However, it is important to rapidly develop plant varieties with desirable characteristics to meet the increasing food demand of the rapidly growing world population, so the application of biotechnological methods integrated into plant breeding and combined with traditional methods can help reduce food shortages. Today, with the quick acceleration of biotechnology, molecular DNA marker technology has been developed in plant breeding and very important developments have been experienced. Thanks to the development of molecular tools for genetic research aimed at improving agricultural traits in plants related to crop yield, crop quality, or tolerance to adverse environmental conditions, we now have a much better understanding of plant genetics and the architecture and function of plant genomes. Therefore, it is of critical importance to revise current breeding procedures by incorporating molecular markers into breeding programs in the future.

Buddleoside-rich Chrysanthemum indicum L. extract modulates macrophage-mediated inflammation to prevent metabolic syndrome induced by unhealthy diet

BackgroundMetabolic syndrome (MetS) is a precursor to the development of many diseases (atherosclerosis, diabetes, etc.). It is marked by disruptions in glucose and lipid metabolism, along with hypertension. Numerous types of risk factors contribute to the development of the MetS, inflammation and insulin resistance are present throughout the metabolic abnormalities. Chrysanthemum indicum L. is a traditional Chinese plant used for both tea and medicine, known for its high content of total flavonoids, which are important secondary metabolites. Our research led to the extraction of a Buddleoside-Rich Chrysanthemum indicum L. extract (BUDE) which has demonstrated anti-inflammatory properties. Nonetheless, the specific role and mechanism of BUDE in preventing MetS remain unclear.MethodsThe study initially evaluated the role of BUDE in preventing MetS. Subsequently, it investigated the anti-inflammatory properties of BUDE in the liver and pancreas in response to unhealthy diets. It then examined the level of insulin resistance and pancreatic β-cell function induced by inflammation. Additionally, an lipopolysaccharide (LPS)-induced macrophage inflammation model was used to further investigate the ameliorative effects of BUDE in inflammation.ResultsBUDE has hypotensive, hypoglycemic and hypolipidemic effects. It can also resolve the imbalance between macrophage subpopulations, impede the triggering of the NF-κB signaling pathway, reduce the secretion of inflammatory mediators, ameliorate insulin resistance, and safeguard organs such as the liver and pancreas from inflammatory damage. These effects collectively contribute to preventing the development of MetS.DiscussionBUDE has the ability to modulate macrophage-mediated inflammation, leading to improved insulin resistance. Additionally, it delivers antihypertensive, hypoglycemic, and hypolipidemic effects, offering a potential for preventing MetS.

Rosa roxburghii Tratt (Cili) has a more effective capacity in alleviating DSS-induced colitis compared to Vitamin C through B cell receptor pathway

Rosa roxburghii Tratt (RRT), a traditional Chinese plant known as the ’King of Vitamin C (VitC; ascorbic acid, AsA)’, contains a wealth of nutrients and functional components, including polysaccharides, organic acids, flavonoids, triterpenes, and high superoxide dismutase (SOD) activity. The various functional components of RRT suggest that it may theoretically have a stronger potential for alleviating colitis compared to VitC. This study aims to verify whether RRT has a stronger ability to alleviate colitis than equimolar doses of VitC and to explore the mechanisms underlying this improvement. Results showed that RRT significantly mitigated body weight loss, intestinal damage, elevated inflammation levels, and compromised barriers in mice induced by Dextran sulfate sodium (DSS). Additionally, RRT enhanced the diversity and composition of intestinal microbiota in these DSS-induced mice. Colon RNA sequencing analysis revealed that compared to VitC, RRT further downregulated multiple immune-related signaling pathways, particularly the B cell receptor (BCR) pathway, which is centered around genes like Btk and its downstream PI3K-AKT, NF-κB, and MAPK signaling pathways. Correlation analysis between microbiota and genes demonstrated a significant relationship between the taxa improved by RRT and the key genes in the BCR and its downstream signaling pathways. Overall, RRT exhibited superior capabilities in alleviating DSS-induced colitis compared to VitC by decreasing intestinal inflammation and modulating BCR and its downstream signaling pathways, potentially regulated by the improved intestinal microbiota.

Parthenolide attenuates hypoxia-induced pulmonary hypertension through inhibiting STAT3 signaling

BackgroundPulmonary hypertension (PH) is a chronic lung disease characterized by the progressive pulmonary vascular remodeling with increased pulmonary arterial pressure and right ventricular failure. Pulmonary vascular remodeling involves the proliferation, migration, and resistance to apoptosis of pulmonary artery smooth cells (PASMCs). Parthenolide (PTN) is a bioactive compound derived from a traditional medical plant feverfew (Tanacetum parthenium), and it has been studied for treatment of pulmonary fibrosis, lung cancer, and other related ailments. However, the function of PTN in the treatment of PH has not been studied. PurposeThis study aimed to evaluate the anti-proliferation and pro-apoptosis effects of PTN on PH and investigate its potential mechanisms. MethodsAn in vivo hypoxia-induced pulmonary hypertension (HPH) model was established by maintaining male rats in a hypoxia chamber (10% O2) for 3 weeks, and PTN was intraperitoneally administered at the dose of 10 or 30 mg/kg. We assessed the impact of PTN on mean pulmonary arterial pressure (mPAP), pulmonary vascular remodeling, and right ventricular hypertrophy. In vitro, we evaluated hypoxia-induced cellular proliferation, migration, and apoptosis of rat PASMCs. Proteins related to the STAT3 signaling axis were analyzed by western blotting and immunofluorescence assays. Recovery experiments were performed using the STAT3 activator, colivelin TFA. ResultsPTN significantly alleviated the symptoms of HPH rats by attenuating pulmonary arterial remodeling. It also prevented the proliferation and migration of PASMCs. PTN also induced the apoptosis of PASMCs. PTN could directly interact with STAT3 and markedly inhibited STAT3 phosphorylation and nuclear translocation. In vitro, and in vivo experiments demonstrated that overexpression of STAT3 partially suppressed the effect of PTN. ConclusionOur study indicated that PTN alleviated hypoxia-induced pulmonary hypertension in rats by suppressing STAT3 activity.

The Effect of Powder Form and Sample Weight on the Acceptability of Yellow Melinjo (Gnetum gnemon L.) Peel Tea

Tea is one of the nutritious drinks that are popular with the Indonesian people. Recently, researchers have developed various new types of tea beyond the traditional tea plant (Camelia sinensis). One potential ingredient for tea development is melinjo peel, particularly the yellow melinjo peel, due to its strong antioxidant activity and superior α-glucosidase inhibitory activity. This study aims to investigate the influence of the powder form and sample weight on the acceptability of yellow melinjo (Gnetum gnemon L.) peel tea. The research employed an experimental method with a factorial completely randomized design (CRD) involving two factors. The first factor was the powder form of the tea (fine, semi-fine, and coarse), and the second factor was the sample weight (2.5 grams, 3 grams, and 5 grams). A hedonic test was conducted to assess the acceptability of yellow melinjo peel tea, involving 40 semi-trained panelists using a scale from 1 to 7 (strongly dislike to very much like). Data were analized using IBM SPSS 25.0 Statistics for Windows, with Kruskal-Wallis and Mann-Whitney tests at a significance level of P &lt; 0.05. The results showed that both the powder form and sample weight of yellow melinjo peel tea significantly affected (P&lt;0.05) taste, aroma, clarity, intensity, astringency, flavor, and overall acceptability, but did not significantly affect (P &gt; 0.05) color and taste intensity. The most preferred formulation by panelists was yellow melinjo peel tea with a sample weight of 2.5 grams and a fine powder form, with an average score of 4.23 (like slightly).

Balancing Yield and Environmental Impact: Nitrogen Management and Planting Density for Rice in Southwest China

With growing concerns about global warming, it is crucial to adopt agronomic practices that enhance rice yields from paddy fields while reducing greenhouse gas (GHG) emissions for sustainable agriculture. An optimal nitrogen (N) fertilization rate and planting density are vital to ensure high rice yields, minimize GHG emissions, and understand emission behavior for better field management. We hypothesized that optimizing N application rates and planting density to improve nitrogen use efficiency (NUE) in rice cultivation would reduce resource losses and GHG emissions. To test this hypothesis, we implemented five treatments with a rice straw return cultural system: two planting densities (16 hills m−2 (traditional density, D1) and 20 hills m−2 (25% higher density, D2)) and three N application rates (no N fertilizer (N0), 180 kg N ha−1 (N1), and 144 kg N ha−1 (N2)). The control treatment (CK) was traditional planting density with no N fertilizer. The four new cropping modes were N1D1, N1D2, N2D1, and N2D2. We investigated the effects of N application rates and planting density on rice grain yield, NUE, and GHG emissions in multiple rice-growing seasons. The N1D2 treatment exhibited the highest grain yield over the three years, with a value of 10,452 kg ha−1, representing an increase of 12.2% compared to CK. Moreover, N uptake in N1D2 was the highest, averaging 39.2% (p &lt; 0.05) higher than CK, and 8.5%, 3.5%, and 2.8% (p &lt; 0.05) higher than N1D1, N2D1 and N2D2, respectively. N2D2 exhibited the highest NUE, with a value of 58.99 kg kg−1, surpassing all other treatments over the three years. GHG emissions, global warming potential (GWP), and greenhouse gas intensity (GHGI) in N2D2 were lower than in N1D1, N1D2, and N2D1. Additionally, reducing N application (comparing N1D1 to N2D1) and increasing plant density (comparing N1D1 to N1D2) improved N agronomic efficiency (NAE) and N partial productivity (PFPN). The negative correlation between the NAE and PFPN with GWP and GHG emissions further supports the potential for optimized N management and denser planting density to reduce environmental impact. These findings have important implications for sustainable rice cultivation practices in Southwest China and similar agroecosystems, emphasizing the need for integrated nutrient management strategies to achieve food security and climate change mitigation goals.

Phylogeny and bioprospecting: The diversity of medicinal plants used in cancer management

Societal Impact StatementAs the second‐leading cause of mortality worldwide, cancer is a major focus of drug discovery research. Traditional plant knowledge can guide the search for undiscovered compounds, but the efficacy of this approach for cancer, a highly complex disease affecting diverse tissues, is unknown. We investigated the patterns underlying plant selection for cancer treatment globally, finding certain lineages are repeatedly targeted. While this indicates therapeutic value, their relatedness with plants used for unrelated ethnobotanical uses suggests that plants are probably selected to treat cancer‐associated symptoms, rather than addressing tumour growth. Careful re‐examination and scoring of ethnobotanical reports may make the prediction of lineages for drug discovery more informative.Summary Cancer is a highly diverse disease and as the second‐leading cause of death worldwide is a focus of drug discovery research. Natural products have been shown to be a useful source of novel molecules for treating cancer. It is likely there are many plants with undiscovered molecules of therapeutic value, however identifying new leads from the vast diversity of plants is very challenging. Traditional knowledge might inform bioprospecting by predicting lineages of plants rich in therapeutically useful molecules. We characterise the phylogenetic diversity of plants used in traditional cancer management using a comprehensive genus‐level phylogeny of angiosperms, and a list of 597 genera used globally to treat different cancers. We phylogenetically predict which lineages may have elevated potential for drug discovery and assess the quality of the prediction. We demonstrate the independent and repeated targeting of specific lineages of plants by different peoples in different parts of the world. However, the lineages we report here as rich in plants used in traditional cancer management coincide with those for other ethnobotanical applications and contain few plants with proven anti‐cancer activity. That the same lineages are used to treat different cancers is suggestive of independent discovery of therapeutic value. However, it is likely that the traditional knowledge explored here is shaped by the selection of plants conferring milder effects for treating wider symptoms, such as tiredness or nausea, rather than for halting tumour growth. Accurate prediction of useful plant lineages for cancer management requires more nuanced information than is commonly provided in ethnobotanical records.

The Heritagescape of Kichwa People of Nizag Built upon Traditional Plant Usage along a Chimborazo Variant of the Andean Road System or Qhapaq Ñan

To document the dynamics of biocultural heritage, we studied traditional uses of plants on a segment of the Andean Road System, or Qhapaq Ñan, within the central Andes of Ecuador, home of the Kichwa community of Nizag. Here, residents preserved a rich diversity of plants within their agricultural fields, or chakra, of the Andean landscapes, upholding a culture steeped in traditional knowledge. Wild and domesticated plants are a cornerstone for the community, prompting the development of ethnobotanical management strategies with a conservation approach. The diverse plant uses aim to revalue their culture, fortify their self-identification as an indigenous community, and ensure the transmission of ancestral knowledge. This research aims to ascertain the ethnobotanical applications of this venerable community. Employing participatory action research, semistructured interviews were conducted with 43 community informants to gather ethnobotanical data on the flora applied within their territory, spanning categories such as food, agriculture, crafts, living fences, construction, fuel, ornamental, environmental, technological, and medicinal services. The findings indicate that age, rather than educational level and gender, significantly influences the variation in plant usage knowledge. In total, 142 plant species were cataloged, comprising herbs (58%), shrubs (23%), trees (15%), and climbers (4%). The most represented families were Asteraceae, Fabaceae, Lamiaceae, and Poaceae. Notably, 96% of these species provide some type of environmental service or fulfill a niche within the socioecological system. Medicinal uses were reported for 89% of the plants, while 56% served agricultural purposes, primarily in fertilizer production and forage. Additionally, 44% of the plants are utilized as food, typically consumed fresh. Among the medicinal plants, leaves are the most used part (58%), prepared as infusions and poultices. The prevalent ailments addressed include respiratory and urinary system conditions, with Borago officinalis, Carica pentagona, Vasconcellea pubescens, and Origanum mejorana being the most frequently employed plants.

Moringa oleifera Lam.: A comprehensive review on its potential for diabetes management and beyond

Diabetes is a metabolic disease characterized by deregulated insulin levels and hyperglycemia. The global healthcare system widely acknowledges the use of medicinal plants as therapeutic agents. One such traditional plant with antidiabetic properties is Moringaoleifera, which has been used for centuries.M.oleifera,due to its important bioactive compounds such asflavonoid, carotenoidandpolyphenols,is used totreatvariousailments,includingdiabetes, cancer, kidney problemsandbacterial and viral infections. This review summarizes the applications of M.oleiferaanditsmechanismsforpreventingdiabetes. Additionally, it explores the influences of M.oleifera in treating diabetes, with a specific focus on its mechanism and effectiveness in reducing insulin levels and managing the conditions. The objectives of this review aretopresentsthe most recent information and elucidate the mechanism by which M.oleiferapreventsdiabetes. Additionally, it provides a thorough analysis of M.oleifera’sapplicationsasanatural treatment and its industrial uses. The reviewhighlightscurrentknowledge gapsandsuggests areas for further research. In conclusion, this study enhancesourunderstanding of the comprehensive applications of M.oleifera,advancing knowledge and help to preventdiabetes-related complications.

A qualitative exploration of food choice motives of urban indigenous food gatekeepers in Fiji: Implications for the use of the food choice questionnaire

Pacific Island Countries (PICs) have exceptionally high rates of obesity and non-communicable diseases. The causes are complex but one prominent factor is the notable shift from traditional plant and seafood diets to diets high in unhealthy processed foods. Literature is sparse on the motives behind food choice decisions of Pacific Islanders. The widely used Food Choice Questionnaire (FCQ) (Steptoe et al., 1995) is a multi-dimensional tool capturing nine potential food choice motives. This study used a hybrid thematic analysis. Fourteen in-depth semi-structured interviews with urban indigenous Fijian mothers were conducted to (1) explore how the nine FCQ motives impact food choice (2) to examine conceptual equivalence of the nine FCQ motives and (3) to identify additional food choice motives, not captured in the FCQ. Mothers identified seven of the nine FCQ motives (i.e., health, mood, convenience, sensory appeal, weight control and familiarity) and three new food choice motives (i.e., food that is filling, religious dietary restrictions and food quality) as important determinants of food choice. Moreover, while choosing foods that promote health and well-being was identified as an important food choice motive, mothers also identified four other motives, that they considered important, which lead to over-eating and unhealthy eating. Food choice is a complex, layered decision, driven by multiple considerations, some more important than others. These findings can inform diet-related interventions and policy within this community. Adapting the FCQ for broader PIC use through further qualitative exploration in PICs, and validating the adapted FCQ with larger samples will enhance its utility in measuring food choice motives in PICs.

Cotton-quality fibers from complexation between anionic and cationic cellulose nanoparticles

Natural polymers are attractive sustainable materials for production of fibers and composite materials. Cotton and flux are traditional plants used to produce textiles with comforting properties while technologies like Viscose, Lyocell and Ioncell-F allowed to extent fiber use into regenerated cellulose from wood. Neither natural nor man-made fibers completely satisfy the needs for cellulose based fabrics boosting development of new approaches to bring more sustainability into the fashion. Technologies like Spinnova are arising based on the spinning of mechanically pretreated cellulose materials with a lower environmental impact though challenged by the fiber quality and strength related to the inconsistency of the mechanical fibers. Nanoscaled cellulose is an excellent solution to improve the consistency of spin fibers, but charges introduced by traditional chemical treatments prevent rebuilding native hydrogen bonding and compromise the mechanical properties especially in wet conditions. We used nanocellulose with low surface charge isolated using reactive eutectic media to spin fibers able to restore the native hydrogen bonding and enable constitutional mechanical strength of cellulose. We performed un-optimized spinning to reveal the intrinsic properties of the fibers and confirmed the preserved strength of wet fibers compliant with the low surface charge enabling further engineering towards cotton-like fabric from wood.