This study proposes a differential wavelength measurement method based on the electromagnetic-induced photoacoustic effect. The differential method involves irradiating the sample with multiple wavelengths and utilizing differences in absorption characteristics across different materials to calculate and measure the excitation light wavelengths. Compared to traditional detection methods, this approach combines the unique properties of electromagnetic-induced photoacoustic effect, offering high sensitivity and a wider detection range from microwave to light. Furthermore, the system is structurally simple and stable, suitable for non-destructive testing of various materials, including wavelength-sensitive biological tissues. The experimental results demonstrate that combined with Polymers Benzodithiophene Triazole–Quinoxaline (PBTQ) and Single-Walled Carbon Nanotubes (SWCNTs) as absorbing media, this technique provides a rapid and cost-effective means of wavelength measurement, achieving an uncertainty of approximately 2.33 nm within the range of 680–800 nm, and it can be used for wavelength/frequency measurement of various electromagnetic waves.