Traditional Delivery Methods Research Articles

Constructability Reviews Utilization Practices for Traditional and Alternative Project Delivery Methods Across State Transportation Agencies

State Transportation Agencies (STAs) recognized that to enhance the quality of construction documents, a review process must be incorporated into project planning, design, and procurement to evaluate projects for constructability. The benefits promised by constructability reviews (CRs) encouraged STAs to adopt it as part of their operations. This approach soon evolved into a structured process, recognized by researchers and practitioners as, the constructability review process (CRP). A significant component to the CRP success is to involve experienced construction personnel in CRs during the design phase; a major limitation of the traditional design-bid-build (DBB) delivery method. To overcome this limitation, more emphasis is being placed towards alternative project delivery methods (APDMs). As such, design–build (DB) and construction manager/general contractor (CM/GC) are among the APDMs most utilized by STAs to deliver transportation projects. Although extensive research has investigated CRs under DBB projects, research on CRs implementation under APDMs is remarkably absent. This study examines CRs utilization and staffing practices adopted by STAs across DBB, DB, and CM/GC projects. The results of this study were drawn utilizing data collected through a national survey questionnaire and interviews with selected STAs. The study found that CRs are initiated and implemented at proportionally similar phases across DBB, DB, and CM/GC projects. Investigation of staffing needs revealed that although the agency holds the executive role in implementation of CRs on DBB projects, their role shifts on DB projects to become more administrative. The agency continues to be involved in CRs under CM/GC projects, along with their allocated design consultant, in conjunction with the GC. The results of this study are anticipated to provide STAs with guidance for CRs utilization on DBB, DB and CM/GC projects.

Formulation, Optimization, In Vitro and In Vivo Evaluation of Saxagliptin-Loaded Lipospheres for an Improved Pharmacokinetic Behavior

The development and optimization of controlled release lipospheres (LS) from safe biocompatible behenic acid (BA) was performed for not only enhancing patient's compliance against highly prevailed chronic diabetes but also to vanquish the insufficiencies of traditional methods of drug delivery. The Box-Bhenken design (BBD) was utilized to statistically investigate the impact of formulation variables on percentage yield (Y1), entrapment efficiency (Y2), and SG-release (Y3) from saxagliptin- (SG-) loaded LS, and the chosen optimized LS were subjected to a comparative in vivo pharmacokinetic analysis against commercially available SG brand. The compatibility analysis performed by DSC and FTIR established a complete lack of interaction of formulation components with SG, while p-XRD suggested a mild transformation of crystalline drug to its amorphous form during encapsulation process. The spherical, free flowing smooth surface LS having zeta potential of -32 mV and size range of 11-20 μm were conveniently formulated. The obtained data for Y1 (30-80%), Y2 (30-70%), and Y3 (40-90%) showed a best fit with quadratic model. The pharmacokinetics analysis of LS showed a significantly decreased Cmax of SG (75.63 ± 3.85) with a sufficiently elevated Tmax (10.53 h) as compared to commercial brand of SG (99.66 ± 2.97 ng/mL and 3.55 ± 2.18 h). The achievement of greater bioavailability of SG was most probably attributed to higher level of half-life, mean residence time (MRT), and AUC0-24 for SG released from LS. Conclusively, the novel approach of SG-loaded LS had successfully sustained the plasma SG level for a prolonged time without increasing Cmax which would ultimately bring an effective management of chronic diabetes.

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Compatibility of a Thermoresponsive and Controlled Release System for Promoting Sinonasal Cilia Regeneration

The current clinical goal for managing chronic rhinosinusitis (CRS), a heterogenous disease of the paranasal sinuses, is to control inflammation, yet adjunct therapies that promote mucosal regeneration can improve the long-term health of the upper airways. The small natural openings to the sinuses, however, limit the efficacy of traditional drug delivery methods (i.e., nasal sprays and irrigation). Accordingly, a conformable thermoresponsive and controlled release system ("TEMPS", Thermogel, Extended-release Microsphere-based delivery to the Paranasal Sinuses) is developed. The poly(lactic-co-glycolic acid) microsphere component enables the encapsulation of numerous therapeutics, such as retinoic acid (RA), an analog of vitamin A (VA). Studies in CRS patients and preclinical models have shown that aqueous RA or VA gels promoted the differentiation of ciliated cells and improved mucosal healing following repeat applications. In the present study, TEMPS is designed for the controlled release of RA such that a single dose of RA-TEMPS delivers bioactive drug for at least 30 days. Furthermore, as TEMPS will be in direct contact with sinonasal tissue, its compatibility with ciliated human nasal epithelium is explored. After ex vivo incubation in thermogel for 24 h, cilia motility is maintained, providing evidence that TEMPS can be compatible for application along the sinonasal epithelium.

Profit distribution of IPD projects using fuzzy alliance

PurposeThis paper examines the profit distribution of engineering projects in the integrated project delivery (IPD) mode. IPD is a new delivery method that can ameliorate many of the disadvantages of traditional delivery methods and improve project results. In the implementation of IPD, the profit distribution is key for ensuring the success of IPD projects.Design/methodology/approachThis paper described a new method for characterizing profit distribution in the IPD mode. The payment function and Shapley value of the cooperative fuzzy game of fuzzy alliance were defined by considering the Choquet integral of the fuzzy measure. The participation of each player was considered, and the influence of participation on the profit distribution was discussed. Lastly, changes in the profit distribution of core participants under different alliance combinations were studied.FindingsA case from a report of The American Institute of Architects (AIA) was used to verify the fuzzy alliance model. There was a significant correlation between the degree of participation of the owner, architect and builder and the profit distribution among these three participants.Research limitations/implicationsThe theoretical research in this paper has some limitations. Initially, this paper selects a case with only three key participants in order to simplify the research. When there are many core participants, how to establish the alliance in the IPD mode and how to establish the corresponding profit distribution model, further work is certainly required to disentangle these complexities in models. Second, in this case, BIM technology has little impact on the income of the whole project. Therefore, this paper does not consider the impact of BIM technology on the marginal effect of the IPD project. Third, the contract type in the case is a custom tri-party based on IFOA. There is no classified discussion of the impact of different contracts on the profit distribute in the paper.Practical implicationsBased on the in-depth study of cooperative game with alliance structure, this paper promotes the classic cooperative game with alliance structure. The authors define the payoff function of fuzzy cooperative games by Choquet integral of fuzzy measure, and introduce the idea into the field of IPD. It aims at extending the solution to a cooperative game without a core. It can be obtained through a simple calculation. In the IPD alliance, the fuzziness and uncertainty of the participation degree of each participant will affect the profit of the whole project. The authors find that the higher the participation rate of players, the more profit each participant has. The greater the influence weight of the designer on the alliance, the lower the influence weight of the contractor on the alliance, the lower the participation of the contractor and the designer, and the lower the income distribution value of the three core participants. It shows a monotonous decline status.Social implicationsFor any construction enterprise, it can make more profits if it joins the grand alliance. In the IPD alliance, each participant can maximize their own interests, which can also promote the enthusiasm of construction enterprises to participate in the alliance and increase the application of IPD mode in AEC industry. This research method provides a new fast, effective, and more realistic solution method for cooperative countermeasures. It can be further extended to other cooperative game fields and advance a new research perspective and solution for the distribution of cooperative interests.Originality/valueThe contribution of this paper is the development of a fuzzy alliance model that provides a tool for measuring the profit distribution in IPD. This is the first quantitative model to connect the degree of participation with the profit distribution in IPD using fuzzy alliance.

Maritime Robotics and Autonomous Systems Operations: Exploring Pathways for Overcoming International Techno-Regulatory Data Barriers

The current regulatory landscape that applies to maritime service robotics, aptly termed as robotics and autonomous systems (RAS), is quite complex. When it comes to patents, there are multifarious considerations in relation to vessel survey, inspection, and maintenance processes under national and international law. Adherence is challenging, given that the traditional delivery methods are viewed as unsafe, strenuous, and laborious. Service robotics, namely micro aerial vehicles (MAVs) or drones, magnetic-wheeled crawlers (crawlers), and remotely operated vehicles (ROVs), function by relying on the architecture of the Internet of Robotic Things. The aforementioned are being introduced as time-saving apparatuses, accompanied by the promise to acquire concrete and sufficient data for the identification of vessel structural weaknesses with the highest level of accuracy to facilitate decision-making processes upon which temporary and permanent measures are contingent. Nonetheless, a noticeable critical issue associated with RAS effective deployment revolves around non-personal data governance, which comprises the main analytical focus of this research effort. The impetus behind this study stems from the need to enquire whether “data” provisions within the realm of international technological regulatory (techno-regulatory) framework is sufficient, well organized, and harmonized so that there are no current or future conflicts with promulgated theoretical dimensions of data that drive all subject matter-oriented actions. As is noted from the relevant expository research, the challenges are many. Engineering RAS to perfection is not the end-all and be-all. Collateral impediments must be avoided. A safety net needs to be devised to protect non-personal data. The results here indicate that established data decision dimensions call for data security and protection, as well as a consideration of ownership and liability details. An analysis of the state-of-the-art and the comparative results assert that the abovementioned remain neglected in the current international setting. The findings reveal specific data barriers within the existing international framework. The ways forward include strategic actions to remove data barriers towards overall efficacy of maritime RAS operations. The overall findings indicate that an effective transition to RAS operations requires optimizing the international regulatory framework for opening the pathways for effective RAS operations. Conclusions were drawn based on the premise that policy reform is inevitable in order to push the RAS agenda forward before the emanation of 6G and the era of the Internet of Everything, with harmonization and further standardization being very high priority issues.

Media Business Development in the Context of Global Digitalization

In the context of global changes in the media market under the influence of Internet technologies, it becomes necessary to identify the main trends of its development, so the characteristic of the market trends, the integration process of entrepreneurial structures in the media industry, aimed at increasing their competitiveness at the present stage of development of the market economy. In the context of an ever-changing, competitive digital ecosystem and against the background of General cuts in advertising budgets in the print press, radio, and television, advertising investments in the digital environment are increasing starting in 2019. Revenues of legacy news media organizations (also called old or traditional media organizations) are constantly declining due to the high degree of audience participation in new media due to the use of user-generated content, media automation, and new digital media organizations. Using mathematical-statistical and logical research methods, the article identifies specific features of the media business sphere, which include audience fragmentation, culture of creative destruction, and hypercompetition in the media market. In addition, key issues and current changes in the media industry in the context of global digitalization are highlighted, reflecting the introduction of innovative technologies that affect the media industry, and the decline in traditional methods of delivery and media consumption. Global digitalization has affected the growth of media platforms, resulting in an increase in the supply of content itself, as well as significant changes in its consumption by the advertising market.

A Review of Digital Cognitive Behavioral Therapy for Insomnia (CBT-I Apps): Are They Designed for Engagement?

There are different ways to deliver Cognitive Behavioral Therapy for Insomnia (CBT-I), of which in-person (face to face) is the traditional delivery method. However, the scalability of in-person therapy is low. Digital Cognitive Behavioral Therapy for Insomnia (dCBT-I) is an alternative and there are tools on the market that are validated in clinical studies. In this paper, we provide a review of the existing evidence-based CBT-I apps and a summary of the published usability-oriented studies of these apps. The goal is to explore the range of interaction methods commonly applied in dCBT-I platforms, the potential impact for the users, and the design elements applied to achieve engagement. Six commercially available CBT-I apps tested by scientifically valid methods were accessed and reviewed. Commonalities were identified and categorized into interactive elements, CBT-I-related components, managerial features, and supportive motivational features. The dCBT-I apps were effectively assisting the users, and the type of interactions promoted engagement. The apps’ features were based on design principles from interactive product design, experience design, online social media, and serious gaming. This study contributes to the field by providing a critical summary of the existing dCBT-I apps that could guide future developers in the field to achieve a high engagement.

Novel Strategy to Combat Antibiotic Resistance: A Sight into the Combination of CRISPR/Cas9 and Nanoparticles.

Antibiotic resistance is a significant crisis that threatens human health and safety worldwide. There is an urgent need for new strategies to control multidrug-resistant (MDR) bacterial infections. The latest breakthrough in gene-editing tools based on CRISPR/Cas9 has potential application in combating MDR bacterial infections because of their high targeting ability to specifically disrupt the drug resistance genes that microbes use for infection or to kill the pathogen directly. Despite the potential that CRISPR/Cas9 showed, its further utilization has been hampered by undesirable delivery efficiency in vivo. Nanotechnology offers an alternative way to overcome the shortcomings of traditional delivery methods of therapeutic agents. Advances in nanotechnology can improve the efficacy and safety of CRISPR/Cas9 components by using customized nanoparticle delivery systems. The combination of CRISPR/Cas9 and nanotechnology has the potential to open new avenues in the therapy of MDR bacterial infections. This review describes the recent advances related to CRISPR/Cas9 and nanoparticles for antimicrobial therapy and gene delivery, including the improvement in the packaging and localizing efficiency of the CRISPR/Cas9 components in the NP (nanoparticle)/CRISPR system. We pay particular attention to the strengths and limitations of the nanotechnology-based CRISPR/Cas9 delivery system to fight nosocomial pathogens.We highlight the need for more scientific research to explore the combinatorial efficacy of various nanoparticles and CRISPR technology to control and prevent antimicrobial resistance.

Crowdsourcing mode evaluation for parcel delivery service platforms

The fast-growing practice of e-commerce implies a strong increase in the urban parcel delivery, which in turn creates significant pressure on last-mile city logistics. Because the crowdsourced delivery offers greater flexibility and requires less capital investment than traditional delivery methods, it has been playing a more crucial role when faced with the growing demand for the urban parcel delivery. With the increasing maturity of the crowdsourced delivery and the fierce competition among platforms, the evaluation of different crowdsourcing modes for the urban parcel delivery becomes important. This study proposes six mathematical models to evaluate different operation modes of the crowdsourced delivery in a quantitative way. Several realistic factors, such as the latest service time for each task, task cancellation rate and range distribution of tasks, are also analyzed in this paper. Numerical experiments are conducted to validate the effectiveness of the proposed models and to analyze the impact of different modes. Some managerial implications are also outlined on the basis of the numerical experiments and sensitivity analysis to help crowdsourced companies to make scientific decisions.

Dental Education in the COVID-19 Era: Challenges, Solutions and Oppor- tunities

Objective: The COVID-19 crisis has caused considerable disruption to medical education and has stimulated creative and rapid changes in the way technology is adopted and utilized for education. This change is accompanied by many challenges related to education, patient care, research, faculty development and collaboration, finances, and the psychological well-being of stakeholders. Methods: A review of the literature on dental education during the COVID-19 pandemic was conducted. Published articles addressing the challenges, solutions and opportunities in dental education during the COVID-19 era were assessed. Results: COVID-19 was a catalyst for a significant amount of change in dental education. Despite the magnitude of the challenges, this pandemic has influenced many positive solutions and opportunities in dental education. Conclusion: The COVID-19 pandemic forced dental educators to rethink models of curricular delivery, as it disrupted traditional delivery methods. The use of technology was adapted to ensure the continuity of education. With that, a number of challenges surfaced that were tackled creatively. Reflecting over the whole experience with COVID-19, the multiple opportunities that have been identified can improve the way we educate our students in the future.

Recent applications and strategies in nanotechnology for lung diseases.

Lung diseases, including COVID-19 and lung cancers, is a huge threat to human health. However, for the treatment and diagnosis of various lung diseases, such as pneumonia, asthma, cancer, and pulmonary tuberculosis, are becoming increasingly challenging. Currently, several types of treatments and/or diagnostic methods are used to treat lung diseases; however, the occurrence of adverse reactions to chemotherapy, drug-resistant bacteria, side effects that can be significantly toxic, and poor drug delivery necessitates the development of more promising treatments. Nanotechnology, as an emerging technology, has been extensively studied in medicine. Several studies have shown that nano-delivery systems can significantly enhance the targeting of drug delivery. When compared to traditional delivery methods, several nanoparticle delivery strategies are used to improve the detection methods and drug treatment efficacy. Transporting nanoparticles to the lungs, loading appropriate therapeutic drugs, and the incorporation of intelligent functions to overcome various lung barriers have broad prospects as they can aid in locating target tissues and can enhance the therapeutic effect while minimizing systemic side effects. In addition, as a new and highly contagious respiratory infection disease, COVID-19 is spreading worldwide. However, there is no specific drug for COVID-19. Clinical trials are being conducted in several countries to develop antiviral drugs or vaccines. In recent years, nanotechnology has provided a feasible platform for improving the diagnosis and treatment of diseases, nanotechnology-based strategies may have broad prospects in the diagnosis and treatment of COVID-19. This article reviews the latest developments in nanotechnology drug delivery strategies in the lungs in recent years and studies the clinical application value of nanomedicine in the drug delivery strategy pertaining to the lung.

A Cache Placement Strategy Based on Entropy Weighting Method and TOPSIS in Named Data Networking

Named data networking (NDN) aims to change the traditional content delivery method and caching by router nodes caching and participating in forwarding. NDN-caching can reduce the expected flood of global data traffic by providing cache storage at intermediate nodes for transmitted content objects, making data broadcasting in an efficient way. In this paper, a novel caching strategy based on entropy weighting method and TOPSIS is proposed for efficient content dissemination to improve NDN’s in-network caching performance. Firstly, the consumer’s request process is modeled by the entropy weighting method and TOPSIS to obtain the best cache node for the cache object according to the real-time status of the node. Secondly, two cache replacement algorithms (composed of an active cache replacement algorithm and a passive cache replacement algorithm) are proposed to reduce the cache redundancy on the delivery path and improve the utilization of data packets in the nodes. Finally, an effective cache mechanism and data packet migration scheme are proposed to further improve the cache performance according to the different types of cache nodes. The performance evaluation shows that the proposed scheme performs better in terms of cache hit rate, latency, and link load compared with some existing strategies.

Stochastic modelling of maintenance flexibility in Value for Money assessment of PPP road projects

Maintenance flexibility has been promoted as a value driver for long-term public–private partnerships (PPPs). However, the value and risk associated with this value driver have not been properly quantified in the Value for Money (VfM) assessment literature. To bridge the gap, a novel stochastic modelling methodology is proposed to characterize the complex interactions among the lifecycle cost (LCC), performance deterioration and maintenance strategies. Four different maintenance strategies are designed to emulate the practice in the traditional and PPP delivery methods. The LCC includes the direct maintenance cost, user cost, residual value, and payment deduction, the last three often being neglected in VfM assessments. Simulation-based optimization and dynamic programming analysis are used to determine the probability distributions of the LCC and the VfM. A hypothetical highway PPP project under an availability payment model is selected as a case study. The results show that maintenance flexibility is indeed able to reduce the LCC for the private party. However, this private efficiency, if not properly regulated, could cause a reduced asset residual value and an increased user cost, making the public party worse off. In addition, for all potential maintenance strategies, the public sector is found to retain significant lifecycle cost risk, largely in the form of user cost.

Evaluation of Integrated Library System (ILS) Use in University Libraries in Nigeria: An Empirical Study of Adoption, Performance, Achievements, and Shortcomings

Objective - The aim of this study was to evaluate Integrated Library System (ILS) use in university libraries in Nigeria in terms of their adoption, performance, achievements, and shortcomings and to propose a rigorous model for ongoing evaluation based on use of candidate variables (CVs) derived from the approach used by Hamilton and Chervany (1981) and from evaluation criteria suggested by Farajpahlou (1999, 2002). Methods - The study adopted a descriptive survey design. Nigeria is made up of six geo-political zones including: North-East (NE), North-West (NW), North-Central (NC), South-South (SS), South-East (SE), and South-West (SW). The population for this study comprised Systems/IT and E-librarians in the university libraries from all six of the geo-political zones of Nigeria. Because of the large number of universities in each of the zones in Nigeria, a convenience sampling method was used to select six universities representing federal, state, and private institutions from each of the six geo-political zones of Nigeria. A purposive sampling method was used to select the Systems/IT and E-librarians who were directly in charge of ILS in their various libraries. Therefore, the sample for this study was made up of 36 Systems/IT and E-librarians from the 36 selected universities in Nigeria. The instrument used to elicit responses from the respondents was an online questionnaire and was distributed through the respondents’ email boxes and WhatsApp. The questionnaire administration received a 100% response rate. Results - Findings revealed that university libraries in Nigeria have made remarkable progress in the adoption and use of ILS for library services. The findings also showed that much has been achieved in the use of ILS in library services. Evidence in the study indicated that the performance of the ILS adopted in the selected university libraries in the area of data entry and currency, accuracy, reliability, completeness, flexibility, ease of use, and timeliness was encouraging. Conclusions - Adoption and use of ILS in libraries is changing the way libraries deliver services to their patrons. Traditional methods of service delivery are different from the expectations of the 21st century library patrons. The transformation seen in the university libraries in Nigeria using ILS was tremendous and is changing the narratives of the past. However, several shortcomings still exist in the adoption and use of ILS in university libraries in Nigeria. Overcoming some of the limitations would require a conscious effort and decisiveness to ensure that librarians and library patrons enjoy the best services that ILS can offer. ILS developers should consider the dynamic needs of libraries and their patrons and incorporate specific candidate variables (CVs) in their ILS designs to enhance the quality of the services being offered to the library patrons.

Sound generation in zebrafish with Bio-Opto-Acoustics

Hearing is a crucial sense in underwater environments for communication, hunting, attracting mates, and detecting predators. However, the tools currently used to study hearing are limited, as they cannot controllably stimulate specific parts of the auditory system. To date, the contributions of hearing organs have been identified through lesion experiments that inactivate an organ, making it difficult to gauge the specific stimuli to which each organ is sensitive, or the ways in which inputs from multiple organs are combined during perception. Here, we introduce Bio-Opto-Acoustic (BOA) stimulation, using optical forces to generate localized vibrations in vivo, and demonstrate stimulation of the auditory system of zebrafish larvae with precise control. We use a rapidly oscillated optical trap to generate vibrations in individual otolith organs that are perceived as sound, while adjacent otoliths are either left unstimulated or similarly stimulated with a second optical laser trap. The resulting brain-wide neural activity is characterized using fluorescent calcium indicators, thus linking each otolith organ to its individual neuronal network in a way that would be impossible using traditional sound delivery methods. The results reveal integration and cooperation of the utricular and saccular otoliths, which were previously described as having separate biological functions, during hearing.

Abstract 17357: Direct Cardiac Reprogramming Using Combinatorial Modified mRNA

Introduction: Despite various clinical modalities, ischemic heart disease remains among the leading causes of mortality and morbidity worldwide. The elemental problem is the immense loss of cardiomyocytes (CMs) post-myocardial infarction (MI). Reprogramming non- cardiomyocytes (non-CMs) into cardiomyocyte (CM)-like cells in vivo is a promising strategy for cardiac regeneration: however, the traditional viral delivery method hampered its application into clinical settings due to low and erratic transduction efficiency. Methods: We used a modified mRNA (modRNA) gene delivery platform to deliver different stoichiometry of cardiac-reprogramming genes (Gata4, Mef2c, Tbx5 and Hand2) together with reprogramming helper genes (Dominant Negative (DN)-TGFβ, DN- Wnt8a and Acid ceramidase (AC)), named 7G, to induce direct cardiac reprogramming post myocardial infarction (MI). Results: Here, we identified 7G modRNA cocktail as an important regulator ofthe cardiac reprogramming. Cardiac transfection with 7G modRNA doubled cardiac reprogramming efficiency (57%) in comparison to Gata4, Mef2C and Tbx5 (GMT) alone (28%) in vitro . By inducing MI in our lineage tracing model, we showed that one-time delivery of the 7G-modRNA cocktail reprogrammed ~25% of the non-CMs in the scar area to CM- like cells. Furthermore, 7G modRNA treated mice showed significantly improved cardiac function, longer survival, reduced scar size and greater capillary density than control mice 28 days post-MI. We attributed the improvement in heart function post modRNA delivery of 7G or 7G with increased Hand2 ratio (7G-GMT Hx2) to significant upregulation of 15 key angiogenic factors without any signs of angioma or edema. Conclusions: 7G or 7G GMT HX2 modRNA cocktails boosts de novo CM-like cells and promotes cardiovascular regeneration post-MI. Thus, we highlight that this gene delivery approach not only has high efficiency but also high margin of safety for translation to clinics.

Multiplex Genome Engineering Methods for Yeast Cell Factory Development

As biotechnological applications of synthetic biology tools including multiplex genome engineering are expanding rapidly, the construction of strategically designed yeast cell factories becomes increasingly possible. This is largely due to recent advancements in genome editing methods like CRISPR/Cas tech and high-throughput omics tools. The model organism, baker’s yeast (Saccharomyces cerevisiae) is an important synthetic biology chassis for high-value metabolite production. Multiplex genome engineering approaches can expedite the construction and fine tuning of effective heterologous pathways in yeast cell factories. Numerous multiplex genome editing techniques have emerged to capitalize on this recently. This review focuses on recent advancements in such tools, such as delta integration and rDNA cluster integration coupled with CRISPR-Cas tools to greatly enhance multi-integration efficiency. Examples of pre-placed gate systems which are an innovative alternative approach for multi-copy gene integration were also reviewed. In addition to multiple integration studies, multiplexing of alternative genome editing methods are also discussed. Finally, multiplex genome editing studies involving non-conventional yeasts and the importance of automation for efficient cell factory design and construction are considered. Coupling the CRISPR/Cas system with traditional yeast multiplex genome integration or donor DNA delivery methods expedites strain development through increased efficiency and accuracy. Novel approaches such as pre-placing synthetic sequences in the genome along with improved bioinformatics tools and automation technologies have the potential to further streamline the strain development process. In addition, the techniques discussed to engineer S. cerevisiae, can be adapted for use in other industrially important yeast species for cell factory development.

Does Psychosocial Functioning Improve With Prolonged Exposure in Veterans With PTSD?: Exploring Traditional and Home-Based Telehealth Delivery Methods.

The current study explored whether prolonged exposure (PE), delivered in person or via home-based telehealth, had a therapeutic effect on psychosocial functioning in combat Veterans with posttraumatic stress disorder (PTSD). The effects of home-based telehealth on these metrics were also evaluated. In addition, we examined whether race, type of war conflict, and service-connected disability rating moderated the effect of PE on psychosocial functioning and whether PTSD, anxiety, and/or depression mediated the effect of PE on psychosocial functioning. We did not find moderating or mediating effects in our study. Improvements in PTSD, depression, and anxiety were associated with improvements in psychosocial functioning. We did not identify statistically significant differences in scores representing change in overall and each domain of psychosocial functioning between groups. Within-group analysis indicated psychosocial functioning improved in both groups but was mostly not statistically significant. However, some clinically relevant improvement may have occurred. [Journal of Psychosocial Nursing and Mental Health Services, 59(2), 31-40.].

Engineered Microneedle Patches for Controlled Release of Active Compounds: Recent Advances in Release Profile Tuning

AbstractTransdermal microneedle patches have attracted significant attention in the medical arena over the last decade as alternatives to traditional drug delivery methods. Microneedle patches are capable of delivering their payloads using burst‐ and sustained‐release mechanisms, or in response to internal/external triggers, to optimize their therapeutic potency. Herein, drug release mechanisms and profiles are reviewed, along with the impact of the cargo release on cytotoxicity and delivery efficacy. The potential applications of microneedles in transdermal treatment, from prophylaxis to therapeutics, are also highlighted.