The results of research on the justification of the parameters of freight with three-section mainline (crew) diesel freight locomotives 3 ТЭ 10 М on the railway section between Marokand – Kattakurgan with the route Samarkand – Navoi – Bukhara on Uzbek railways are presented, that were obtained by the famous locomotive traction theory. The values of parameters of main indicators of freight with studied diesel freight locomotives 3ТЭ10М were specified as a result of the performing the series of grade computations for different conditions of the organization of freight traffic on the section Marokand – Kattakurgan. Moreover, these values are presented in tabular data and graphic dependencies. Average and arithmetic mean values of main indicators are determined with the regard to freight trains without stopping and with stopping on interstation. The regression equations are obtained that intended for the calculation of averaged parameters’ values of the main indicators for fuel and energy efficiency to utilize the studied diesel freight locomotives 3 ТЭ 10 М in real conditions of the organization of rail freight transportation for the accepted range of mass’ variation of freight trains. The results of research are recommended for the practical utilization by locomotive drivers-instructors and specialists of the linear locomotive enterprises on the Uzbek railway network whose professional and production activities relate to issues of the diesel fuel saving for the haulage of trains.