Toxic Formaldehyde Research Articles

Permeability-Controlled Probe for Ratiometric Detection of Plasma Membrane Integrity and Late Apoptosis.

The destruction of plasma membrane integrity is closely related to immune response, neuronal injury, cell apoptosis, and other pathological events. However, the construction of ratiometric fluorescent probes capable of detecting plasma membrane integrity remains a significant challenge, hindering in-depth studies on related biomedical areas. Herein, a polarity-responsive fluorescent probe was constructed for the ratiometric detection of cell membrane integrity for the first time. The probe targeted intact plasma membranes in healthy cells and relocated into the cytoplasm to give significantly red-shifted fluorescence after plasma membrane damage. Molecular simulations revealed that the high transmembrane barrier and amphipathic nature of the probe were responsible for its targeting ability. With the probe, the ratiometric detection of late apoptosis stage was realized for the first time, and the membrane damage of tumor cells induced by UV irradiation, toxins, and antitumor drugs was visualized. The effect of formaldehyde on membrane integrity was evaluated using a probe, and cysteine was proved to be a potential detoxifier to counteract the toxicity of formaldehyde.

Sustainable Production of Dialkoxymethane Ethers and Related Acetals Using CO2, H2 and Alcohols or Diols

AbstractDialkoxymethane (DAM) ethers, so‐called oxymethylene ethers (OMEs), are compounds constituted by a CH2‐unit bound to two identical alkoxy substituents. These compounds have showcased interesting technological applications as fuel additives or fuels, as a consequence of their interesting properties such as their low vapor pressure, high viscosity, cetane number and oxygen content. Moreover, they are also employed as environmentally‐benign solvents and bulk compounds useful in fine‐chemical industry. In addition, DAM ethers are considered formaldehyde surrogates. Industrially, dimethoxymethane (DMM) is still produced via two‐step protocol involving toxic formaldehyde that consists of a first step of methanol oxidation (Formox process). Hence, the development of more sustainable protocols for their synthesis is highly desired. Among the different strategies reported for DAM synthesis, its production from CO2/H2 and alcohols constitutes a practical, green and attractive alternative route. In this concept, a careful discussion of the developed catalytic protocols for synthesizing DAM from CO2/H2 or formic acid/H2 and alcohols/diols will be summarized. A special emphasis will be performed on the reaction mechanisms involved and the catalyst nature design and characterization.

Evaluation of the long-term effects of formaldehyde on the physiology of the Mediterranean mussel, Mytilusgalloprovincialis

Formalin baths are the most widely used treatment for ectoparasitic fish diseases. Nonetheless, their use in fish cages has been blamed for a number of problems. Although a considerable amount of literature has been produced on the short-term toxic effects of formaldehyde, there is virtually no data on the long-term side effects of the compound on non-target organisms. Therefore, the purpose of this research was to assess the long-term formaldehyde toxicity in Mediterranean mussel, Mytilus galloprovincialis, a common sentinel species that inhabits the area surrounding cage farms. Mussels were kept in a laboratory microenvironment at 20 ± 1 °C for 21 days and exposed to two different formaldehyde concentrations during experimentation: a low dose (L; 40 ppb) based on formaldehyde field measurements in the vicinity of Mediterranean cages, and a high dose (H; 400 ppb) generated by a factor of 10 of the previous dose. A multi-biomarker approach that included antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD), lipid peroxidation (MDA), lysosomal stability (NRRT), genotoxicity tests, condition index (CI), and stress on stress (SoS), was used to evaluate the toxicity of formaldehyde on mussels. The results of the selected tests indicate that formaldehyde does not cause chronic toxicity in mussels subjected to commonly measured concentrations in the aquatic environment following formalin bath treatments. Despite being defined as reversible, the stress brought by the high dose used seems to reduce the antioxidant activity of the tested organism. The significance of this research lies in its contribution to understanding the wider ecological effects of formaldehyde exposure. Moreover, the results highlight the need for further research on other non-target marine organisms to fully understand the cumulative effects of formaldehyde on marine ecosystems.

Estimation of Formaldehyde Levels in Indoor Air of Gross Anatomy Laboratory

Introduction: Medical students spend majority of their times in Anatomy lab during their first year MBBS. Formalin has attracted attention as a health hazard for students and instructors as formalin concentrations in air of gross anatomy laboratories are often higher than permissible limits. Despite the known toxicity of formaldehyde and its potential health effects, laboratory workers and others have little enthusiasm for reducing or replacing the formaldehyde working solution Materials and Methods: The FA concentration (in ppm) of indoor air in Anatomy laboratory was measured using a SMILEDRIVE Portable air quality pollution meter. The mean FA concentration was measured after 1 hour after the cadavers were shifted to dissection table at three different levels a. At the level of floor b. At the level of roof c. 2 feets above the table (Breathing Zone of medical students) Three air samples from the breathing zones were tested and mean concentration of FA was taken with exhaust fan on at the level of roof and recorded as a. S1R- First immediately before the dissection (Zero hour), b. S2R- Second sample was collected after one hour after first sample was taken (First hour) and c. S3R- Third sample after two hours after first sample was taken (Second hour). Three air samples from the breathing zones were tested and mean concentration of FA was taken with exhaust fan on at the level of floor and recorded as a. S1F- First immediately before the dissection (Zero hour), b. S2F- Second sample was collected after one hour after first sample was taken (First hour) and c. S3F- Third sample after two hours after first sample was taken (Second hour). Results: The vertical distribution of FA was measured by taking the mean air samples at the level of floor, at roof level and about 2 feet above dissection table immediately after the cadavers were shifted to tables. It was found to be about 0.54ppm at the level of floor, 0.52 at the breathing zone and 0.51 ppm at roof level. The FA concentration was highest at the level of floor and least at the level of roof. On comparing S2F and S2R the p value was 1.335. Thus, it was not statistically significant. On comparing S3F and S3R the p value was 0.411. Thus, it was not statistically significant. Conclusion: The mean FA at the level of floor was 0.54 ppm. At the breathing zone the mean FA concentration was 0.51 ppm before the dissection was started. Exhaust fans at the level of roof were switched on and the second sample was taken after 1 hour. The FA concentration was 0.23 ppm. Third sample was taken at 2nd hour and FA concentration was 0.11 ppm. The exhaust fans at the level of floor were switched on. The air samples were measured at 0, 1st and 2nd hour at breathing zones. It was 0.51, 0.31 and 0,15 ppm respectively.

Distinct DNA repair mechanisms prevent formaldehyde toxicity during development, reproductionand aging.

Formaldehyde (FA) is a recognized environmental and metabolic toxin implicated in cancer development and aging. Inherited mutations in the FA-detoxifying enzymes ADH5 and ALDH2 genes lead to FA overload in the severe multisystem AMeD syndrome. FA accumulation causes genome damage including DNA-protein-, inter- and intra-strand crosslinks and oxidative lesions. However, the influence of distinct DNA repair systems on organismal FA resistance remains elusive. We have here investigated the consequence of a range of DNA repair mutants in a model of endogenous FA overload generated by downregulating the orthologs of human ADH5 and ALDH2 in C. elegans. We have focused on the distinct components of nucleotide excision repair (NER) during developmental growth, reproduction and aging. Our results reveal three distinct modes of repair of FA-induced DNA damage: Transcription-coupled repair (TCR) operating NER-independently during developmental growth or through NER during adulthood, and, in concert with global-genome (GG-) NER, in the germline and early embryonic development. Additionally, we show that the Cockayne syndrome B (CSB) factor is involved in the resolution of FA-induced DNA-protein crosslinks, and that the antioxidant and FA quencher N-acetyl-l-cysteine (NAC) reverses the sensitivity of detoxification and DNA repair defects during development, suggesting a therapeutic intervention to revert FA-pathogenic consequences.

Electrophoretic Microplate Protein Identification Based on Gold Staining of Molybdenum Disulfide Hydrogels.

Numerous high-performance nanotechnologies have been developed, but their practical applications are largely restricted by the nanomaterials' low stabilities and high operation complexity in aqueous substrates. Herein, we develop a simple and high-reliability hydrogel-based nanotechnology based on the in situ formation of Au nanoparticles in molybdenum disulfide (MoS2)-doped agarose (MoS2/AG) hydrogels for electrophoresis-integrated microplate protein recognition. After the incubation of MoS2/AG hydrogels in HAuCl4 solutions, MoS2 nanosheets spontaneously reduce Au ions, and the hydrogels are remarkably stained with the color of as-synthetic plasmonic Au hybrid nanomaterials (Au staining). Proteins can precisely mediate the morphologies and optical properties of Au/MoS2 heterostructures in the hydrogels. Consequently, Au staining-based protein recognition is exhibited, and hydrogels ensure the comparable stabilities and sensitivities of protein analysis. In comparison to the fluorescence imaging and dye staining, enhanced sensitivity and recognition performances of proteins are implemented by Au staining. In Au staining, exfoliated MoS2 semiconductors directly guide the oriented growth of plasmonic Au nanostructures in the presence of formaldehyde, showing environment-friendly features. The Au-stained hydrogels merge the synthesis and recognition applications of plasmonic Au nanomaterials. Significantly, the one-step incubation of the electrophoretic hydrogels leads to high simplicity of operation, largely challenging those multiple-step Ag staining routes which were performed with high complexity and formaldehyde toxicity. Due to its toxic-free, simple, and sensitive merits, the Au staining integrated with electrophoresis-based separation and microplate-based high-throughput measurements exhibits highly promising and improved practicality of those developing nanotechnologies and largely facilitates in-depth understanding of biological information.

Resorcinol‐Phthalaldehyde Resins for Photosynthesis of Hydrogen Peroxide: Modulation of Electronic Structure and Integration of Dual Channel Pathway

AbstractModulating of electronic structure of photocatalyst and integration of dual channel pathway are promising strategy for efficient photosynthesis of hydrogen peroxide (H2O2) from pure water without sacrificial agent and oxygen exposure. In this work, nontoxic aromatic dialdehyde is used to replace the commonly used toxic formaldehyde to form resorcinol‐phthalaldehyde resins through a hydrothermal method. The introducing of aromatic ring as π spacer increases the separation distance of electrons and holes to avoid their recombination. H2O2 can be produced via integrated oxygen reduction reaction (ORR) and water oxidation reaction (WOR) due to the suitable energy band positions and spatially separated reaction sites. Resorcinol‐p‐phthalaldehyde (RP) resin exhibits a promising H2O2 yield of 3351 µmol g−1 h−1 with high apparent quantum yield (AQY) of 14.9% at 420 nm and solar‐to‐chemical conversion (SCC) efficiency of 1.54%. This work provides a simple strategy to modulate the charge separation efficiency and redox reaction pathway on photocatalyst at molecular level design.

Recent Development in Synthesis of N‐Methylamines with Amines and Methanol

AbstractThe N‐methylation reaction of amines with methanol via the borrowing‐hydrogen strategy is an important method for the construction of C−N bonds. Compared with other C1 sources, such as toxic formaldehyde or expensive formic acid, methanol is more suitable for N‐methylation reactions due to its eco‐friendliness and low price. Using this strategy, various types N‐methylated products, including biological and pharmaceutical molecules have been prepared. Here, we present a thorough survey of decades of articles showing current developments in N‐methylation reactions with methanol. We discuss homogenous, heterogenous and light‐driven catalytic systems for both N‐methylation and N,N‐dimethylation reactions. This review presents the shortcomings of current developments and provides future development progress in N‐methylation reactions with methanol.

What are the DNA lesions underlying formaldehyde toxicity?

Formaldehyde is a highly reactive organic compound. Humans can be exposed to exogenous sources of formaldehyde, but formaldehyde is also produced endogenously as a byproduct of cellular metabolism. Because formaldehyde can react with DNA, it is considered a major endogenous source of DNA damage. However, the nature of the lesions underlying formaldehyde toxicity in cells remains vastly unknown. Here, we review the current knowledge of the different types of nucleic acid lesions that are induced by formaldehyde and describe the repair pathways known to counteract formaldehyde toxicity. Taking this knowledge together, we discuss and speculate on the predominant lesions generated by formaldehyde, which underly its natural toxicity.

Use of Ozone Therapy and Thymoquinone in the Prevention of Formaldehyde Toxicity by Inhalation: An Experimental Study.

The study determined the damage caused by formaldehyde (FA) exposure in blood and liver samples using biochemical markers. Histopathological analysis was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method and measurement of CD68 cell density. To what extent the antioxidant molecules thymoquinone (TQ) and ozone (O3) reversed the damage caused by FA exposure was investigated, both when used alone and combined. Fifty-six Sprague-Dawley male rats of eight to ten weeks of age were used in the experiment. The rats were divided into eight groups, with seven rats in each group: the untreated control group, the group treated with TQ (10 mg/kg/day), the group treated with O3 (150 μg/kg/day), the group treated with TQ+O3, the group exposed to FA (10 ppm 8 h/day), the group receiving FA+TQ, the group receiving FA+O3, and the group receiving FA+TQ+O3.Serum aspartate transaminase (AST), alanine transaminase (ALT), total antioxidant (TAS, U/mL), and total oxidant (TOS, nmol/mL) levels were analyzed. TAS and TOS levels, CD68 cell density, and apoptotic cells were determined in liver tissues. FA exposure caused an increase in serum AST and ALT levels of (p<0.05) experimental animals, a decrease in TAS levels in serum (p=0.03) and liver (p>0.05) and an increase in TOS levels (p>0.05), TUNEL positivity (p<0.001), and CD68 cell density (p=0.004). Administration of TQ and O3as antioxidants significantlyreversed biochemical and histopathological alterations in the serum and liver. TQ and ozone therapy suppressed oxidative stress caused by FA exposure and reversed the emerging histopathological deteriorations. Ozone therapy did not suppress the effects of TQ. Therefore, ozone therapy can be given as a supportive therapy along with the main therapeutic agents. We think TQ and ozone therapy may be useful to protect individuals exposed to FA.

Green tea marinades can reduce formaldehyde of pan-fried pork via Mannich reaction mechanism

Foods containing proteins or unsaturated fatty acids can generate toxic formaldehyde upon exposure to high thermal. Polyphenol-fortified food has been proved to be has less heat-induced toxic aldehydes formation (including formaldehyde), however, the chemical mechanism underlie such effect has not been fully revealed. In the present research, whether Mannich reaction mechanism is responsible for natural polyphenols's inhibition on formaldehyde production on cooking meat was explored. It is validated that green tea marination can suppress the accumulation of formaldehyde of pan-fried porks. By using HPLC, LC-ESI-MS and LC-ESI-HRMS analysis, members of catechins from tea polyphenols were found has high activity for Mannich addition. Besides, a serial of polyphenol-formaldehyde-amino acid three-component adducts were found in the extract of green tea marinated pork. Herein, we proposed firstly that tea polyphenols' inhibitory effect on formaldehyde was accounted for (at least in part) the formation of Mannich adducts.

Converting formaldehyde in methanol with MoO2 under irradiation: A pollution-free strategy for cleaning air

Direct photocatalytic reduction of toxic formaldehyde (HCHO) in value-added chemicals and fuels is promising because that not only abates the environmental pollution, but also solves the energy shortage. Herein, self-supported MoO2 and MoO3 nanoparticles growing on Mo meshes were comparatively applied to the photocatalytic conversion of HCHO. Under UV–visble lights, MoO2 reduces HCHO in methanol (CH3OH) while MoO3 oxidizes HCHO in carbon oxide and water. Their contrary photocatalytic capacities were revealed. Compared with MoO3, the lower work function of MoO2 enables an electron-rich interface, realizing a complete reduction of 30 ppm HCHO to CH3OH in 30 min. Theoretical calculations clarify that a large number of delocalized electrons on MoO2 attracts HCHO molecule and activates its CO bond, facilitating subsequent hydrogenation and reduction of HCHO to CH3OH. As for MoO3, the wider bandgap and higher potential of valence band govern the photocatalytic oxidation of HCHO.

Fabrication of PS-DVB@Cu core-shell microsphere for anisotropic conductive adhesives by electroless plating with copper nanoparticles as seeds

Polymer@metal core-shell microspheres are widely used in anisotropic conductive adhesives (ACAs). Styrene-divinylbenzene copolymer (PS-DVB) copper core-shell microspheres (PS-DVB@Cu) with high conductivity and anti-oxidation performance were successfully prepared by combining copper nanoparticles (Cu NPs) and electroless plating copper. The Cu NPs served as catalytic seeds, substituting noble metals to promote electroless deposition. Using environmentally benign ammonia borane as the reductant to replace toxic formaldehyde, the dense copper film was deposited on the surface of PS-DVB microspheres. The thickness of the Cu layer and bulk electrical resistivity of PS-DVB@Cu were tuned by controlling the concentration of the reducing agent. The minimum resistivity of PS-DVB@Cu was 3.27×10−6 Ω·m and merely slightly changed in the air for two months. The prepared PS-DVB@Cu-based ACAs could be effectively applied to anisotropic conductive connections in small-scale circuits with satisfactory anisotropic conductivity.

Ultraviolet enhanced inorganic graphenylene-like ZnMgX2 (X=O, S) for sensitive and reversible detection of toxic formaldehyde at room temperature: A first-principles study

This study investigates the interaction between formaldehyde (HCHO) and newly predicted graphenylene-like ZnMgX2 (X=O, S) monolayers based on first-principles calculations. It was found that the adsorption performance of HCHO molecule on pure ZnMgX2 monolayers was favorable and exothermic in nature, exhibiting no need for the presence of a metal support. Their experimental feasibility was proven through various analyses, including the evaluation of cohesive energy, phonon dispersion and ab initio molecular dynamics (AIMD) simulations. The results of the topological analysis indicate that the examined monolayers are stabilized by the mixed ionic-covalent bond. By systematically analyzing the adsorption energy, charge transfer, band gap, work function and presence of competing gases (H2O, N2, O2 and H2), their detection sensitivity and response times were determined. Under the presence of ultraviolet (UV) irradiation, the ZnMgO2 system demonstrates suitability as an electronic sensor for HCHO detection at room temperature, while ZnMgS2 with its large adsorption energy has the potential to be used for scavenging HCHO. Further investigation into the adsorption of H2O and O2 yields supporting evidence for high selectivity and improved recovery time of HCHO and potential formation of hydroxyl (•OH) and superoxide (O2•−) radicals, thereby establishing favorable conditions for eventual catalytic degradation of HCHO. This study will serve as a theoretical foundation for the development of HCHO gas sensors intended for both small-scale and industrial applications.

Atypical K6-ubiquitin chains mobilize p97/VCP and the proteasome to resolve formaldehyde-induced RNA-protein crosslinks

In this issue of Molecular Cell, Rahmanto etal.1 and Zhao etal.2 demonstrate that RNA-protein crosslinks contribute to formaldehyde toxicity by blocking protein synthesis. Furthermore, they identify a ubiquitin-mediated degradation system for RNA-protein crosslink resolution in eukaryotes.

Sensing Behavior of Pristine and TM-decorated Zn12O12 Nanocage Towards Toxic Formaldehyde, Phosgene and Thiophosgene Gases

Sensing Behavior of Pristine and TM-decorated Zn12O12 Nanocage Towards Toxic Formaldehyde, Phosgene and Thiophosgene Gases

Preparation and characterization of plywood panels using recycled HDPE as a formaldehyde-free adhesive: A comparative study

Formaldehyde-based resins such as urea-formaldehyde (UF) are the most widely used adhesives in the plywood industry. However, due to the concerns about formaldehyde toxicity, there is a need to develop formaldehyde-free adhesive materials. Therefore, the present study aimed to minimize or eliminate the harmful effects of UF resin by using recycled high-density polyethylene (HDPE) from post-consumed fruit packing boxes as a nontoxic adhesive. Three-layer plywood panels using poplar veneers and recycled HDPE films were prepared. UF was used as a control adhesive for the comparison. The influence of three variable parameters in terms of HDPE content (from 80 to 320 g/m2), hot-pressing temperature (from 140 to 180 °C), and pressing duration (from 3 to 8 min) on the properties of plywood panels were evaluated. For evaluation, physical properties, namely water absorption (WA), thickness swelling (TS), and mechanical properties, namely modulus of elasticity (MOE), modulus of rupture (MOR), and glue-line shear strength of the samples, were studied. The findings of this work confirmed that HDPE films gave the values of mechanical properties of plywood panels comparable to those obtained with UF adhesive, even though the adhesive content and pressing time were lower than when the UF adhesive was used. The obtained bonding strength values of HDPE-bonded plywood panels ranged from 0.82 to 1.60 MPa and met the requirement for the European Standard EN 314-2. According to FE-SEM micrographs, the fractured surface of plywood panels with 240 g/m2 of HDPE displayed a homogeneous cross-section area, and a large amount of HDPE was attached to the veneer surfaces. It was found that the average mechanical properties tended to increase with an increase in the pressing temperature and time. The type of adhesive had a significant effect on WA and TS. The smallest values of WA and TS were observed in plywood samples using high contents of HDPE films (240 and 320 g/m2). The optimum conditions were an HDPE loading of 240 g/m2, a hot-pressing temperature of 160 °C, and a hot-pressing time of 5 min. The overall results showed that recycled HDPE had enough potential to reuse as a promising adhesive for the environmentally-friendly plywood manufacturing.

Silencing of peroxiredoxin III inhibits formaldehyde-induced oxidative damage of bone marrow cells in BALB/c mice.

Formaldehyde (FA) is associated with the occurrence of leukemia, and oxidative stress is considered to be a major reason. As an endogenous biomarker of oxidative stress, few studies focus on the relationship between peroxiredoxin III (PrxIII) and FA toxicity. Our previous research observed high expression of PrxIII occurred in the process of apoptosis of bone marrow cells (BMCs) induced by FA, however the exact mechanism is unclear. Therefore, this paper aimed to explore the possible association between FA toxicity and PrxIII gene. We first, used a Cell Counting Kit-8 (CCK-8) to detect the viability of BMCs after they were exposed to different doses of FA (50, 100, 200 μmol/L) for different exposure time (12, 24, 48 h), then chose 24 h as an exposure time to detect the expression of PrxIII for exposing different doses of FA by Quantitative reverse transcription-PCR (qRT-PCR) and Western blot analysis. Based on our preliminary experimental results, we chose 100 μmol/L FA as an exposure dose to expose for 24 h, and used a small interfering RNA (siRNA) to silenced PrxIII to examine the cell viability by CCK-8, reactive oxygen species (ROS) level by DCFH-DA, apoptosis by Annexin V/PI double staining and cell cycle by flow cytometry (FCM) so as to explore the possible regulatory effect of PrxIII silencing on FA-induced bone marrow toxicity. High expression of PrxIII occurred in the process of FA-induced oxidative stress. Silencing of PrxIII prevented FA from inducing oxidative stress, thus increasing cell viability, decreasing ROS level, rescuing G0 -G1 and G2 -M arrest, and reducing cell apoptosis. PrxIII silencing might be a potential target for alleviating FA-induced oxidative damage.

Improving the Efficiency of the Indoor Air Purification from Formaldehyde by Plants Colonized by Endophytic Bacteria Ochrobactrum sp.

The endophytic bacteria can be in symbiosis with host plants, owing to the natural stability advantages in degrading pollutants. To explore the technological feasibility of this method for indoor formaldehyde removal, a system combining endophytic bacteria and plants was established. In the present study, highly efficient formaldehyde-degrading bacteria Ochrobactrumintermedium, named strain ZH-1, was successfully induced with antibiotics (rifampicin) to an antibiotic-labeled strain ZH-1R without microbial variation. The strain ZH-1R was then used for colonization in the Epipremnum aureum and Chlorophytum comosumf. variegate plants by three inoculation methods: root irrigation (RI), acupuncture injury to stem (AS), and acupuncture injury to leaves (AL). The results demonstrated that the acupuncture injury to stem (AS) method was the most effective for inoculating ZH-1R strain in Epipremnum aureum plants. Conversely, acupuncture injury to stem (RI) method yielded the best results for the Chlorophytum comosumf. variegate plants, highlighting the importance of usage of optimal plant specific inoculation method ensuring the highest possible performance characteristics of the biological system. The results of 8-day formaldehyde dynamic fumigation experiment demonstrated that the removal efficiency of the formaldehyde by Chlorophytum comosum f. variegata inoculated with ZH-1R was significantly higher than the one demonstrated by non-inoculated plants. The average increase of 20.17% was observed during daytime, while much more significant improvement by 62.88% was achieved at night. This implied that endophytic bacteria could not only effectively improve the removal efficiency of formaldehyde, but also increased the resistance of not-native host plants to formaldehyde toxicity, suggesting its potential in an integrated system which provides a new path of an efficient and economical approach to radically improve indoor air quality, especially at nighttime.

Sestec adhesive based on animal proteins for the production of particleboards

The development of a biodegradable adhesive that uses waste from industry will bring enormous economic, social, environmental, and health benefits. Many laboratories around the world conduct research on the development of natural adhesives that can replace those based on toxic formaldehyde. Works in this area are also carried out by Sestec Polska Sp. z o. o, an example of which is the Sestec B0021G adhesive. This product belongs to the family of animal protein-based adhesives and is dedicated to particle board (PB) production (classes P1–P5 according to EN 312). This work presents the results obtained during the preparation of boards based on Sestec B0021G protein adhesive, manufactured with the use of variable production parameters, and assesses the influence of these parameters on the properties of the obtained products. The emphasis was especially on presenting the impact of production parameters on the PB strength and water resistance. The tests were carried out using different densities (560–800 kg/m3), pressing temperatures (150–230 °C), amounts of adhesive (7–15% in the surface layer and 5–13% in the core layer) and specific press times (5–13 s/mm). The boards showed excellent mechanical properties and water resistance. Higher PB classes were obtained under standard production conditions. Eventually, the obtained results showed that the binder can be used to produce all classes of PB products P1 to P7, and not only P1-P5 as initially assumed.