Toxic Baits Research Articles

The effectiveness of Fipronil active substances compared with imidacloprid to control Germany’s cockroaches (Blatella germanica L.)

Blattella germanica L. is a residential pest that can be a disease vector. One of the ways to control Blattella germanica is using toxic baiting. Fipronil and imidacloprid are chemicals used to kill cockroaches. The aim of this study is to compare the effectiveness of the active substances Fipronil and imidacloprid used to control the Blattella germanica. The methods of this study were test insects preparation, making toxic bait, toxic bait effectiveness test, mortality and data analysis calculation. The data were analyzed statistically using One Way ANOVA test and probit analysis. ANOVA test results obtained a significance value of 0.00 (P < 0.05), this indicated that there was a significant difference in the mortality percentage between the treatment groups. The highest deaths percentage occurred in the Blattella germanica group that was treated with a combination of bait and the active substance Fipronil. In addition, the lowest LT50 and LT90 occurred in the LT50 = 28.23 hours and LT90 = 72.10 hours. These results showed that the active substance Fipronil was more effective in controlling the Germany’s cockroach compared to imidacloprid.

The effectiveness of the fipronil and imidacloprid active substances to control weaver ants (Oecophylla smaragdina)

One of the methods used in controlling Oecophylla smaragdina is to use baiting. The purpose of this study was to analyze comparison the effectiveness of the fipronil and imidacloprid active substances to control Oecophylla smaragdina. The methods used in this study include the preparation of test insects, making of toxic bait, test the effectiveness of toxic baits, calculation of mortality and data analysis. ANOVA test results obtained a significance value of 0.00 (P < 0.05), this indicates that there is a significant difference in the percentage of mortality between the treatment groups. The results showed that the two active substances could cause 100% mortality in weaver ants. In addition, the lowest LT50 and LT90 occurred in the use of bait with fipronil, namely LT50 = 6.31 hours and LT90 = 11.21 hours. The active substance fipronil only takes 12 hours to cause weaver ants to experience 100% mortality. In the group of weaver ants who were given bait with a combination of the active substance imidacloprid, the ants died 100% after 24 hours of treatment. The results of this study show that the active substance fipronil is more effective in controlling Oecophylla smaragdina compared to the active substance imidacloprid.

Sugar Bait Composition Containing Ivermectin Affect Engorgement and Mortality of the Mosquito Aedes aegypti (Diptera: Culicidae).

Toxins and attractants have been studied and used in sugar baits for mosquitoes. However, little importance has been given to the combination of sugar concentration, bait composition, and their relationship with mosquito engorgement and mortality. Therefore, the present study evaluated the effects of three concentrations of sucrose on baits with and without an attractant (concentrated guava juice), on engorgement and mortality rates of adult Aedes aegypti (Linnaeus, Diptera: Culicidae). Toxic sugar baits (TSB) and attractant toxic sugar baits (ATSB) containing 10, 50, and 70% sucrose and 100 ppm ivermectin (IVM) were prepared to assess engorgement and mortality rates. Subsequently, different concentrations of IVM (0.312-100 ppm) in TSB and ATSB were prepared with sucrose concentrations of 10 and 70% to determine the lethal concentrations (LC50 and LC90) values. Engorgement on the baits was observed under a stereomicroscope, and mortality was followed up to 48 h after feeding. In general, more mosquitoes engorged on TSB regardless of the sugar concentration, while higher concentrations of sugar in ATSBs resulted in higher numbers of mosquitoes engorging. A large increase in the LC90 of IVM was observed for females feeding on ATSBs and TSBs with 70% sucrose relative to those feeding on baits with lower sugar concentrations. No such effect was observed for males.

How low can you sow? House mouse eradication on Motuareronui/Adele Island

House mice (Mus musculus) are highly invasive mammals and can cause extensive ecosystem damage on islands where they are the sole mammalian pest species. Capability to eradicate mice has improved in recent years. Mouse eradication has been achieved on large islands where mice cohabit with other rodents and islands where mice are the sole mammalian pest. As the islands targeted for eradication become larger and more challenging, reduced toxic cereal bait application rates can reduce both complexity and cost, and ultimately make currently unachievable operations feasible.

Feral cats on Rakiura Stewart Island: population attributes and potential eradication tools

As a major threat to New Zealand’s biodiversity, feral cats (Felis catus) are the subject of planned eradications on a number of offshore islands, including Rakiura Stewart Island. We used camera traps to estimate population density of feral cats on the north-east coast of Rakiura, and to investigate their movement behaviour and detection probability. We also used camera footage to compare the consumption of two types of non-toxic sausage baits (chicken and rabbit) with a view to future use of toxic baits.

Indirect control of the ground-pearl Eurhizococcus brasiliensis (Wille, 1992) (Hemiptera: Coccomorpha: Margarodidae) using ants toxic baits

Linepithema micans (Forel, 1908) (Hymenoptera: Formicidae) is the main ant species responsible for spreading the ground pearl, Eurhizococcus brasiliensis (Wille, 1992) (Hemiptera: Margarodidae). The effect of different formulations of hydramethylnon toxic baits (TB) was evaluated on the control of L. micans and E. brasiliensis. Four formulations of TB were evaluated in a greenhouse to control L. micans. Based on the greenhouse experiment results, the small pellet formulation of the TB was selected to be evaluated, through weekly applications, in two vineyards naturally infested by L. micans and E. brasiliensis. Later, in order to establish the number of applications, 1, 3, 6 and 10 applications in the season were evaluated. The bait treatments were compared with the number of ants and ground pearls counted on one area free of TB and one area with the drench application of thiamethoxam. Hydramethylnon (0.5%) TB in the small pellet (SP) and large pellet (LP) formulations were the most efficient in L. micans control. The SP TB, applied six times during the vine cycle, effectively control the number of L. micans, reducing 100% of the ant population, as well as the E. brasiliensis, reducing 99.9% of the scale population on plants.

Local perceptions and sociocultural value of Hooded Vultures Necrosyrtes monachus in Burkina Faso, West Africa

Hooded Vultures have suffered from dramatic population declines in recent decades owing to anthropogenic pressures. To properly address this threat, the sociocultural perspectives towards this species should be considered. We examined peoples' perceptions, knowledge and beliefs on Hooded Vulture's sociocultural values in Burkina Faso through face to face surveys at 20 sites spread across the country. A total of 900 interviews (with 600 householders and 300 butchers) revealed valuable knowledge and an overall positive perception of Hooded Vultures. More than 72% (n = 653) of interviewees (mostly Mossi, Bissa and Gourmatche ethnic groups) regarded Hooded Vultures as important, venerated and sacred birds, and we received no reports of Hooded Vultures attacking livestock-which is all beneficial to their conservation. Conversely, a minority of interviewees (12%, n = 108) believed that Hooded Vultures are harmful, but the reasons provided for this view were insubstantial. Nevertheless, the negative views towards Hooded Vultures should be urgently examined. Furthermore, the vast majority of respondents (84%, n = 753) viewed Hooded Vulture populations as having decreased and the main reasons provided were decreasing food availability, intentional killing using poisoned baits for beliefmbased use, habitat loss or degradation, and unintentional poisoning. Interestingly, most respondents (84%, n = 760) were acutely aware of the imminence of the Hooded Vulture's possible extinction and are willing to take conservation measures, suggesting both support and eventual success for conservation actions in this area. The key actions suggested by local communities are valuable for vulture conservation and include severely punishing poachers, supplying safe food for Hooded Vultures, preserving and restoring habitat, and raising awareness of Hooded Vulture conservation.

The different aspects of attractive toxic baits containing fipronil for control of the German cockroach (Blattella germanica).

The use of Attractive Toxic Baits (ATBs) is considered to be a low-risk practical method for controlling cockroaches. This study evaluates the attractiveness of a lab-made, fipronil-containing bait, and its effect on the food consumption and mortality of the German cockroach Blattella germanica, under field and laboratory conditions. Different developmental stages of the cockroach were used to determine their preferred carbohydrate/protein rich foods and examine the effectiveness of lab-made baits. The analysis of variance (ANOVA) with Tukey's Test was determined using SAS 9.1 and GraphPad Prism software programs. The significance level was considered at p<0.05. The most-consumed foods were carbohydrate-rich foods (biscuit and banana powder) and food consumption rate was highest in non-gravid females. The most attractive bait ingredients were 20% roasted peanut butter, 50% biscuit and 30% banana powder. The highest lethality was recorded from the baits containing 0.02% fipronil under laboratory conditions, while infested houses baited with the lab-made bait showed 76.5% and 100% decline, respectively, in cockroach numbers in the first- and fourth-week post-baiting. In conclusion, palatable foods with a pleasant odour, like biscuit powder, banana powder and peanut butter were the most effective ingredients for the ATBs. The ATBs impregnated with 0.02% fipronil provide a promising approach for control of the German cockroach. However, there is a need to evaluate the potentials of the lab-made baits, under laboratory and field conditions, in the control of other health-important cockroaches.

An efficient and biodegradable alginate-gelatin hydrogel beads as bait against Aedes aegypti and Aedes albopictus

The effective control of Aedes mosquitoes using traditional control agents is increasingly challenging due to the presence of insecticide resistance in many populations of key mosquito vectors. An alternative strategy to insecticides is the use of toxic sugar baits, however it is limited due to short-term efficacy. Alginate-Gelatin hydrogel beads (AGHBs) may be an effective alternative by providing longer periods of mosquito attraction and control, especially of key vectors of dengue viruses such as Aedes aegypti and Aedes albopictus. Sodium alginate (ALG) and gelatin (GLN) are natural polymers, which can be a potential candidate to develop the AGHBs baits due to their biodegradability and environmental safety. Here we provide an assessment of the preparation of AGHBs optimized by varying the concentrations of ALG, GLN, and its cross-linking time (TIME). Fourier transform infrared spectroscopy (FTIR) analysis results in the determination of liquid bait loaded in the AGHBs. The evaluation of AGHBs' effectiveness as the potential baiting tool based on the mortality rate of mosquitoes after the bait consumption. The 100 % percent mortality of Aedes mosquitoes was obtained within 72 h of bait consumption. The field evaluation also justifies the applicability of AGHBs for outdoor applications. We conclude that the AGHBs are applicable as a baiting tool in carrying liquid bait in achieving mosquito mortality.

Dry and unwary are best conditions for baiting wild pigs (Sus scrofa)

Wild pigs (Sus scrofa) are a highly destructive invasive species throughout North and South America, Australia, and many island nations. Where invasive, their populations are targeted for control to reduce damage. Controlling wild pigs often involves baiting to draw them into traps or entice them to consume a toxic bait. However, baiting can have mixed success in congregating wild pigs to focal areas long enough for control measures to ultimately be implemented. We sought to evaluate how environmental conditions (i.e., precipitation) and negative stimuli (i.e., proxy for exposure to previous control efforts) influenced use of bait sites by wild pigs. We compared visitation to bait sites during dry (2019–2020) and wet (2021) years, and between wild pigs that had been previously trapped and released in southcentral Alabama and northcentral Texas, USA. We found that drier years substantially increased use of bait sites by wild pigs (i.e., 119–136 % increase over 17 days). Similarly, wild pigs that did not experience negative stimuli had increased use of bait sites (i.e., 30–31 % increase over 17 days). We recommend that managers intensify their control efforts during drier periods to take advantage of susceptible behaviors of wild pigs during these times. We also recommend that control efforts focus on eliminating the potential for surviving wild pigs which may experience negative stimuli (e.g., narrow misses during trapping, shooting some wild pigs from a group, sub-lethal doses from toxic baits) and become educated against future efforts.

Fox and cat responses to fox baiting intensity, rainfall and prey abundance in the Upper Warren, Western Australia

Context Invasive predators are major drivers of global biodiversity loss. Red foxes (Vulpes vulpes) and feral cats (Felis catus) have contributed to the decline and extinction of many native species in Australia. The deployment of poison baits to control fox populations is a widespread conservation tool, but the effects of baiting intensity, rainfall and prey abundance on baiting effectiveness remain poorly understood. Aims We aimed to understand what influences the association between fox baiting intensity, red fox activity and feral cat activity, to provide inferences about what might affect the effectiveness of fox baiting in reducing fox activity. Methods We used generalised linear models to assess how fox and cat activity changes in relation to fox baiting intensity, rainfall, native prey availability and distance to agricultural land over a 6-year period (2006–13) in the forest ecosystems of the Upper Warren region of south-western Australia. Key results We found that fox activity was negatively associated with rainfall in the previous 12 months and positively associated with prey abundance and fox baiting intensity. We also found an interaction between fox baiting and prey abundance, with fox activity increasing with prey activity in areas of low and moderate baiting intensity, but remaining constant in areas of high baiting intensity. Feral cat activity was positively associated with prey abundance and fox baiting intensity. We found no clear relationship between fox and cat activity. Conclusions The drivers of the association between fox baiting and fox activity are unclear because intense fox baiting was targeted at areas of known high fox abundance. However, our results indicate that intense fox baiting may be effective at decoupling the positive association between fox activity and prey abundance. Our results also suggest a positive association between fox baiting intensity and feral cat activity, thus supporting the case for integrated fox and cat management. Implications We caution interpretation of our results, but note that management of invasive predators could be improved by adjusting the intensity of management in response to changes in environmental conditions and local context (e.g. strategically conducting intense predator management where prey abundance is highest). Improved understanding of these associations requires a monitoring program with sufficient replication and statistical power to detect any treatment effects.

Potential of ivermectin as an active ingredient of the attractive toxic sugar baits against the Indian malaria vectors Anopheles culicifacies and Anopheles stephensi.

Attractive toxic sugar bait (ATSB) is a novel vector control tool that exploits the sugar feeding behavior of mosquitoes. The current study aims to evaluate the efficacy of ivermectin-based ATSB against insecticide susceptible and resistant strains of major Indian malaria vectors - Anopheles culicifacies and Anopheles stephensi. ATSB with different concentrations of ivermectin were tested against mosquito vectors under standard laboratory conditions. Dose-response analysis of ivermectin-ATSB showed 7.8 and 19.8ppm as 50% and 90% lethal concentration (LC50 and LC90 ) values for insecticide susceptible An. culicifacies. In the case of insecticide susceptible An. stephensi, the LC90 value was 35 ppm which was significantly higher in comparison to the LC90 for An. culicifacies. The LC50 of insecticide-resistant An. culicifacies and An. stephensi were 10.6 and 15.9ppm respectively whereas LC90 values were 36.9 and 61.0. Ivermectin-ATSB resulted in 99 ± 0.8% mortality of An. culicifacies and 93 ± 3.8% mortality of An. stephensi at an ivermectin concentration of 25 ppm. In another set of experiments, the ATSB solution containing standardized dose of ivermectin was sprayed on Allysum plant and mortality of both Anopheline vectors was recorded. Here, we observed > 90% mortality in both An. stephensi and An. culicifacies. Our study demonstrates the potential of ivermectin-based ATSB in killing Indian malaria vectors irrespective of the method of application. Further field trials with ivermectin containing ATSB may pave the way for its usage in the national vector control program. © 2022 Society of Chemical Industry.

Quantitative evaluation of the effects of bycatch on native species using mathematical models

Traps and poison bait are used to control invasive species and protect native species. However, the traps can kill native species and the poison bait may increase their mortality. As it interferes with conservation efforts, it is necessary to evaluate the effects of such bycatch. However, there are few theoretical methods for quantification of these effects.This paper presents a method for quantitatively evaluating the effects of bycatch on native populations, using a mathematical model that represents the population dynamics of native and invasive species.The mathematical model suggested the conditions under which only invasive species will be eliminated even if bycatch occurs. We also present the concept of the ”bycatch curve”, which shows the population density according to the killing rate. In addition, a mathematical simulation is presented using parameters estimated for the Amami-Oshima system.These theoretical results allowed us to determine the parameters that should be prioritized for estimation in actual control programs.

Selecting thermal tolerant strains of entomopathogenic fungi to control Ceratitis capitata (Wiedeman) in tropical semi-arid conditions

Although it is usual, using constant temperature to select thermo-tolerant entomopathogen (EF) strains could discard potentially efficient biocontrol agents (BCA). The objectives of this work were to select virulent EF against C. capitata and evaluate the effect of continual and fluctuating temperature on fungal development and virulence. Initial experiments compared the effect of constant temperatures (CT) (20, 25, 30, 35, and 38 °C) and simulated intraday temperature variation (SIV) (20–38 °C) over conidial germination, mycelial growth, and insect mortality. Temperatures ≥ 35 °C in the CT experiments significantly reduced conidial germination, and the strains of Beauveria bassiana BbLCB81 and BbLCB289 showed the highest conidial germination and mycelial growth. SIV also caused a significant effect, and M. anisopliae MaLCB62 showed the highest conidial germination and mycelial growth. Applying EF strains as toxic baits in CT showed that the highest mortality occurred at 30 °C, and BbLCB62 and BbLCB289 showed the largest insect mortality at 38 °C. Survival analysis showed a slight increase in the average survival time (ST50) at 30 °C. In SIV conditions, MaLCB56 and BbLCB289 were highly virulent. In a field cage experiment at a warmer temperature, BbLCB62 was the most virulent strain according to the M-C test comparing survival curves. However, confirmed accumulated mortality on the 10th day was similar to the moderately tolerant BbLCB289 (Tukey, p < 0.05). During winter, all EF strains showed similar mortality on 10th day (Tukey, p < 0.05), but a significant difference in the survival analysis. EF strains with mild temperature tolerance can be applied in seasons with high temperatures, and BbLCB62 and BbLCB289 were selected as potential biocontrol agents against C. capitata.

Efficacy of Matrine, Rotenone, and Pyrethrin Against Red Imported Fire Ant Solenopsis invicta (Hymenoptera: Formicidae) and Their Impact on Aquatic Organisms.

Chemicals, including toxic bait and dusts, are the main means of controlling the red imported fire ant Solenopsis invicta (abbreviation 'RIFA'), which is a serious invasive pest. To identify environmentally friendly chemicals for controlling RIFA, we tested the toxicity and horizontal transfer of three botanical insecticides-matrine, rotenone, and pyrethrin-and their impact on aquatic organisms (i.e., three fish and one shrimp). The LD50 value of matrine, rotenone, and pyrethrin was 0.24, 50.929, and 13.590 ng/ant, respectively. Matrine, rotenone, and pyrethrin had effective horizontal transfer and caused significant secondary mortality. After exposure to donor workers, 90.75%, 90.75%, and 100% of recipient workers in the 0.025% matrine, 1.0% rotenone, and 0.3% pyrethrin dust treatments, respectively, died within 48 h. Furthermore, 0.025% matrine dust caused significant tertiary mortality (49.5%). Tertiary mortality caused by 1.0% rotenone and 0.3% pyrethrin dusts was very low, only 7.75% and 18.5%, respectively. In a field trial, the comprehensive mortality effect of 0.05% matrine, 1.50% rotenone, and 0.375% pyrethrin dust was 74.96%, 30.10%, and 29.27%, respectively, after 14 d of treatment. Matrine had low toxicity to the fish Hypophthalmichthys molitrix, and 37.5 mg/L pyrethrin had low toxicity to the fish Cirrhinus molitorella, H. molitrix, and Oreochromis niloticus. However, rotenone was highly toxic to all three fish. The shrimp Neocaridina denticulate was not sensitive to matrine, rotenone, or pyrethrin. Matrine dust has highly effective horizontal transfer, and 0.05% matrine dust has great potential for managing RIFA in organic farms, aquaculture farms of H. molitrix, and water resource regions.

Production and Potential Application of Pyroligneous Acids from Rubberwood and Oil Palm Trunk as Wood Preservatives through Vacuum-Pressure Impregnation Treatment.

The development of low-environmental-impact technologies for the elimination of biological damage is one of the vital goals of the wood protection industry. The possibility of utilizing pyroligneous acid as a wood preservative can be a great solution to extend the application of the currently fast-growing timber species, which has lower natural durability against biological damage. In this study, the effectiveness of pyroligneous acid as a wood preservative was evaluated by impregnating rubberwood with pyroligneous acid using vacuum-pressure treatment, and the treated woods were exposed to mould fungi, wood-decay fungi and termite attacks under laboratory conditions. Pyroligneous acids produced from rubberwood (RWPA) and oil palm trunk (OPTPA) at different pyrolysis temperatures were evaluated. To fully understand the effectiveness of pyroligneous acids as wood preservatives, different concentrations of pyroligneous acids were impregnated into rubberwood. Concentrations of 50% RWPA and 30% OPTPA were sufficient against mould and decay fungi on rubberwood. Rubberwood impregnated with pyroligneous acid acted as a slow-acting toxic bait to cause a high termite mortality rate due to toxic feeding and does not serve as a good repellent to prevent termites from feeding on the wood. In general, OPTPA has better biological durability compared to RWPA.

Unraveling the real magnitude of illegal wildlife poisoning to halt cryptic biodiversity loss

Illegal wildlife poisoning is a global threat for biodiversity, yet the magnitude of its impact on ecosystems is largely underestimated as most of poisoning episodes remain undetected. Here, we conducted a large-scale field experiment to better understand the real dimension of the illegal wildlife poisoning in terms of composition and number of species and abundance of impacted individuals, as well as the ecological factors driving it. We used camera traps to monitor simulated poison baits placed in 25 study areas in SW Europe and applied Good–Turing theory to estimate the richness of species of the entire assemblage (observed plus undetected). We recorded 3095 individuals from 39 vertebrate species that consumed 94 % of the baits (N = 590). Yet, using sample completeness to estimate the entire species assemblage yielded a total of 47 species exposed to illegal poisoning. The observed assemblage included different trophic and functional groups (from lizards and snakes to apex species among birds and mammals), as well as a 38 % of threatened and near threatened species (according to Spanish and Portuguese vertebrate red list and UICN list). The size (weight) of the bait outstands as a reliable predictor of the number of species (0–8 species/bait, mean = 2) and individuals (0–99 individuals/bait, mean = 5) susceptible to poisoning. The habitat where the bait was placed modulated the abundance of individuals affected (greater in open than in closed habitats). Type of bait and habitat drove the compositional variation of species. Our approach enables uncover entire species assemblages prone to illegal poisoning and their ecological drivers associated, advancing the understanding of the impact of wildlife poisoning in ecosystems.

Ingestion of spinosad-containing toxic sugar bait alters Aedes albopictus vector competence and vectorial capacity for dengue virus.

Dengue virus (DENV) is a highly prevalent vector-borne virus that causes life-threatening illnesses to humans worldwide. The development of a tool to control vector populations has the potential to reduce the burden of DENV. Toxic sugar bait (TSB) provides a form of vector control that takes advantage of the sugar-feeding behavior of adult mosquitoes. However, studies on the effect of ingestion of toxins in TSB on vector competence and vectorial capacity for viruses are lacking. This study evaluated vector competence for DENV serotype-1 of Aedes albopictus at 7 and 14 days post-ingestion of TSB formulated with spinosad (of bacteria origin) as an oral toxin. Our results and others were modeled to estimate effects on Ae. albopictus vectorial capacity for DENV. Ingestion of TSB caused a reduction in survival of females, but increased mosquito susceptibility to DENV infection, disseminated infection, and transmission. However, this increase in vector competence was obviated by the reduction in survival, leading to a lower predicted vectorial capacity. The findings of this study highlight the importance of evaluating the net impact of TSB ingestion on epidemiological parameters of vectorial capacity in the context of vector control efforts to reduce the risk of transmission of vector-borne viruses.

Rapid, novel screening of toxicants in poison baits, and autopsy specimens by ambient mass spectrometry.

Animal poisoning and dissemination of baits in the environment have public health and ethological implications, which can be followed by criminal sanctions for those responsible. The reference methods for the analysis of suspect baits and autopsy specimens are founded on chromatographic-based techniques. They are extremely robust and sensitive, but also very expensive and laborious. For this reason, we developed an ambient mass spectrometry (AMS) method able to screen for 40 toxicants including carbamates, organophosphate and chlorinated pesticides, coumarins, metaldehyde, and strychnine. Spiked samples were firstly purified and extracted by dispersive solid phase extraction (QuEChERS) and then analyzed by direct analysis in real time high-resolution mass spectrometry (DART-HRMS). To verify the performance of this new approach, 115 authentic baits (n = 59) and necropsy specimens (gastrointestinal content and liver, n = 56) were assessed by the official reference methods and combined QuEChERS-DART-HRMS. The agreement between the results allowed evaluation of the performances of the new screening method for a variety of analytes and calculation of the resultant statistical indicators (the new method had overall accuracy 89.57%, sensitivity of 88.24%, and a specificity of 91.49%). Taking into account only the baits, 96.61% of overall accuracy was achieved with 57/59 samples correctly identified (statistical sensitivity 97.50%, statistical specificity 94.74%). Successful identification of the bitter compound, denatonium benzoate, in all the samples that contained rodenticides (28/28) was also achieved. We believe initial screening of suspect poison baits could guide the choice of reference confirmatory methods, reduce the load in official laboratories, and help the early stages of investigations into cases of animal poisoning.

Laboratory evaluation of sugar alcohols for control of mosquitoes and other medically important flies

Insecticide application for vector control is the most controversial component of a public health program due to concerns about environmental and human health safety. One approach to overcome this challenge is the use of environmentally benign active ingredients. Among the most promising emerging strategies are attractive toxic sugar baits. Sugar alcohols—naturally occurring molecules safe for human consumption but potentially toxic to insects when ingested, have received increased attention for use with this approach. For this study, we screened the toxicity of four different sugar alcohols on several mosquito species, a biting midge, and a filth fly. Sugar alcohol mortalities exceeded those in the sucrose (positive control) only group. However, only erythritol and highly concentrated xylitol induced mortalities exceeding those in the water only (negative control) treatment ranging from approximately 40–75%. Formulations containing erythritol and xylitol should be further investigated under field conditions for efficacy in reducing populations of biting flies and for assessing potential non-target impacts.