This paper deals with the fire resistance behavior and failure mode of transmission towers exposed to wildfires. A nonlinear thermo-mechanical coupling model of a latticed transmission tower was established by the finite element (FE) program ANSYS, which was validated from steel members’ perspectives. Wildfire analysis was conducted to determine the key parameters, including the number of legs in fire, fire intensity, and residence time, and the temperature–time curve was further proposed for parametric analysis. It reveals that the transmission tower may collapse under serviceability state loads in the wildfire, and the final failure mode was determined by the number of tower legs in the wildfire. A higher wildfire intensity will significantly reduce the wildfire resistance time of the transmission tower. Therefore, combustible materials around transmission towers need to be regularly cleaned to reduce the intensity and residence time of wildfires, which is an effective way to prevent wildfires. Moreover, a wildfire protection layer design process was proposed based on the environment surrounding the transmission tower, which proved reliable to provide valuable insights for the fire protection design of transmission towers in areas prone to wildfires.