Ecotourism in protected areas plays an important role in establishing mutually beneficial relationships among local people, the protected area, and tourism that are essential in protected area management. However, to properly manage protected areas, local people should be major stakeholders in order to maximize local economic benefits and obtain support for conservation efforts. This study assesses the current status of local people's economic participation in tourism in the Wolong Nature Reserve using a questionnaire survey. Through evaluation of geographic origin, income, and occupational distribution of operators engaged in tourism-related business, we identified and discussed constraints and opportunities for economic participation of local people. Economic inequity was found among local people, as well as between locals and non-locals, due to limited startup capital and operational skills. At present, only a small percentage of local people receive revenue directly from tourism. In addition, economic leakage and local dependence on natural resources still exist in the study area. To promote ecotourism and sustainable development, the relationships among tourism, local people, and biodiversity conservation in the study area must be strengthened. According to the findings, some suggestions are offered to protected area managers to foster better relationships.