Objective: This study aims to investigate the cooperation and collaboration among the actors involved in the tourist routes known as Salamarias, Della Cuccagna, and Topiarias, Flores e Aromas, located in the northern region of Rio Grande do Sul, Brazil. Theoretical Framework: This article examines the economic and social impact of rural tourism, focusing on its contribution to economic growth, job creation, and the promotion of tourism products through tourist routes. It emphasizes cooperation among tourism actors for sustainable development and organizational efficiency. Method: We conducted qualitative and quantitative research, collecting data through interviews with 16 farms across three routes. A structured interview script included 60 open-ended questions and six closed questions. We analyzed the data using content analysis, and assessed the quantitative data using a five-point Likert scale to measure agreement levels. Results and Discussion: The results showed that cooperation and intercooperation along the routes promote income complementarity through tourism offerings and product sales, enhance knowledge and learning, expand social relations, increase the dissemination and consumption of organic products, and support the preservation of culture, heritage, and the environment. Research Implications: The practical and theoretical implications of this study provide insights that can guide public policies, regional development programs, and business strategies in the rural tourism sector. These insights emphasize the importance of cooperation, intercooperation, social networks, rural development, and social innovation. Originality/Value: The discussion not only enriches the existing literature but also opens new avenues for future research and theorizing in the context of rural tourism and regional development.