Total Unsaturated Fatty Acids Research Articles

Effect of Buffalo Breed on the Detailed Milk Composition in Guangxi, China.

Buffalo is the second source of milk in the world, and its milk is rich in nutritive components. It is well-known that breed influences milk composition. This work aimed to compare the detailed milk composition of three buffalo breeds (Murrah, Nili-Ravi, and Mediterranean) housed under the same environmental conditions. Mediterranean buffalo milk showed a significantly higher content of fat, protein, and some fatty acids. Moreover, the milk from the Mediterranean breed was characterized by the highest content of sphingomyelin (SM), cholesterol, and lanosterol. However, the Murrah buffalo milk contained the highest amount of total unsaturated fatty acids, phosphatidylinositol, and whey proteins. Furthermore, the Nili-Ravi buffalo milk was characterized by the highest content of total saturated fatty acids, phosphatidylglycerol, squalene, lathosterol, stigmasterol, beta-sitosterol, and casein fractions. Nevertheless, the lactose and amino acid profiles of the milk remained almost similar across the three buffalo breeds. The generated results in this study enable a comprehensive understanding of the milk constituent variability that is linked to buffalo breeds, which may support the acquirement of essential scientific knowledge on milk ingredient-processing interactions that will offer a foundation of knowledge for Chinese dairy processors in terms of milk processability and innovation.

A Comparative Study on the Carcass and Meat Chemical Composition, and Lipid-Metabolism-Related Gene Expression in Korean Hanwoo and Brindle Chikso Cattle.

The objective of this study was to elucidate the effect of cattle breed on carcass and meat chemical composition, fatty acid profiles, and lipid-metabolism-related genes. For this study, same-age Hanwoo and Chikso steers (n = 6 per breed) reared under identical conditions were used. Immediately after slaughter, muscle tissues were collected for analysis of mRNA expression. At 24 h post-mortem, the carcasses were assessed for carcass traits (marbling score, meat yield, etc.), and meat quality and fatty acid profiles in the longissimus lumborum (LL) and semimembranosus (SM) muscles. The results showed that no differences in the slaughter weight, dressing rate, back-fat thickness, trimmed fat, and total meat yield occurred between the two breeds (p > 0.05). However, Hanwoo cattle had a higher marbling score, intramuscular fat (IMF) content, and expression level of lipid-metabolism-related genes such as lipoprotein lipase, peroxisome proliferator-activated receptor gamma, and fatty acid binding protein 4, compared with Chikso (p < 0.05). Contrastingly, Chikso had a higher total unsaturated fatty acid content and expression level of stearoyl CoA desaturase 1 (p < 0.05). It may be said that the difference in the expression levels of lipid-metabolism-related genes could be the molecular factors underlying IMF deposition and fatty acid profile differences in the beef from the two breeds.

The evaluation of the genotype and slaughter weight effect on meat quality and fatty acid profile from two native sheep.

The purpose of this study is to investigate the quality characteristics and fatty acid composition of meat from male Akkaraman (AKK) and Bafra × Akkaraman B1 (BA B1) lambs slaughtered at different live weights. Thirty-six male lambs (18 AKK and 18 BA B1) were slaughtered at three different slaughter weights (35, 40, and 45kg) after being fattened intensively. Meat physical quality characteristics and fatty acid profiles were used as response variables. Color parameters (L*, a*, and b*) and pH were not significantly influenced by genotype and slaughter weight (SW). Water-holding capacity (WHC) was significantly affected by SW (P < 0.05), cooking loss (CL) by genotype (P < 0.001). Increasing SW, intramuscular fat of the Longissimus thoracis muscle showed increased total unsaturated fatty acids (TUFA) (P < 0.01) and ratios of TUFA/saturated fatty acids (SFA) (P < 0.01), and decreased thrombogenic and atherogenic indices (P < 0.01). Monounsaturated fatty acids (MUFA) (P < 0.05) and polyunsaturated fatty acids (PUFA) (P < 0.01) varied on with the genotypes. In conclusion, genotype and SW did not greatly affect meat quality; however, MUFA and PUFA mutually changed according to the genotype, and fatty acid percentages improved according to the SW.

The Effects of Different LED Lights on the Main Nutritional Compositions of Isochrysis zhanjiangensis

Light irradiation plays an important role in the growth of microalgae and their nutrient composition. To elucidate the effect of different LED lights (red, orange, green, blue, purple, simulated sunlight, and white) on the main nutritional compositions of Isochrysis zhanjiangensis, the growth, fatty acid composition, soluble carbohydrate, and soluble protein contents were studied. The results show that I. zhanjiangensis grew the fastest under blue light (p = 0.044). In terms of fatty acid composition, the percentage of myristic acid (C14:0) decreased significantly (p = 0.021) under blue light and simulated sunlight, but the percentages of palmitic acid (C16:0) (p = 0.032) and stearic acid (C18:0) (p = 0.037) significantly increased. The percentage of docosahexaenoic acid (C22:6n-3) increased under orange light (p = 0.021), and the percentage of total unsaturated fatty acids increased under blue light (p = 0.008). The protein content significantly increased under simulated sunlight (p = 0.025), while the carbohydrate content did not vary much (p &gt; 0.05) under different lights. These findings provide useful guidance for optimizing the cultivation conditions of I. zhanjiangensis to improve its nutritional value and yield.

Glucose-6-Phosphate Dehydrogenase Activity in Milk May Serve as a Non-Invasive Metabolic Biomarker of Energy Balance in Postpartum Dairy Cows

Negative energy balance (EB) postpartum is associated with adverse outcomes in dairy cows; therefore, non-invasive biomarkers to measure EB are of particular interest. We determined whether specific metabolites, oxidative stress indicators, enzyme activity, and fatty acid (FA) profiles in milk can serve as indicators of negative EB. Forty-two multiparous Holstein dairy cows were divided at calving into 2 groups: one was milked 3 times daily and the other, twice a day for the first 30 d in milk (DIM). Cows were classified retrospectively as being in either negative EB (NEB, n = 19; the mean EB during the first 21 DIM were less than the overall median of -2.8 Mcal/d), or in positive EB (PEB, n = 21; the mean EB was ≥-2.8 Mcal/d). The daily milk yield, feed intake, and body weight were recorded individually. Blood samples were analyzed for metabolites and stress biomarkers. Milk samples were taken twice weekly from 5 to 45 DIM to analyze the milk solids, the FA profile, glucose, glucose-6-P (G6P), G6P-dehydrogenase (G6PDH) activity, malic and lactic acids, malondialdehyde (MDA), and oxygen radical antioxidant capacity (ORAC). The NEB cows produced 10.5% more milk, and consumed 7.6% less dry matter than the PEB cows. The plasma glucose concentration was greater and β-hydroxybutyrate was lower in the PEB vs. the NEB cows. The average concentrations of milk glucose, G6P, malic and lactic acids, and MDA did not differ between groups; however, the G6PDH activity was higher and ORAC tended to be higher in the milk of NEB vs. the PEB cows. The correlation between milk G6PDH activity and EB was significant (r = -0.39). The percentages of oleic acid and total unsaturated FA in milk were higher for the NEB vs. the PEB cows. These findings indicate that G6PDH activity in milk is associated with NEB and that it can serve as a non-invasive candidate biomarker of NEB in postpartum cows, that should be validated in future studies.

Physicochemical and sensory characteristics of traditional Kırklareli meatballs with added cowpea (Vigna unguiculata) flour

The effects of cowpea (Vigna unguiculata) flour on some physical, chemical, textural and sensory properties of Kırklareli meatballs were investigated. For this purpose, cowpea flour was added to the traditional Kırklareli meatball formulation in four different proportions (2, 4, 6 and 8%). As a result of the analysis on cowpea flour; pH, water activity (aw), moisture, protein, fat, ash, carbohydrate, acidity, starch, total dietary fiber, total monounsaturated fatty acids, total polyunsaturated fatty acids, total unsaturated fatty acids and total saturated fatty acids were found as 6.25, 0.52, 10.20, 20.35, 0.53, 2.94, 65.43%, 0.06, 45.09, 20.90, 5.86, 59.88, 65.74 and 34.26%, respectively. Depending on the increase in incorporation rate of cowpea flour, changes in L* (43,40-53,88), b* (13,92-18,11), pH (5,83-5,94), aw (0,96-0,98), moisture (44,03-50,63%), protein (17,70-21,89%), fat (19,49-22,97%), carbohydrate (6,77-12,11%), salt (1,28-1,74%), total dietary fiber (2,81-5,08%) values of the raw samples and a* (5,64-9,44), b* (9,77-18,06), moisture (39,27-45,24%), protein (19,92-23,45%), fat (23,08-26,19%), carbohydrate (5,92-11,30%), total dietary fiber (3,28-5,40%) values of the cooked Kırklareli meatball samples were statistically significant (P < 0.05), while the changes in weight loss, ash, free fatty acidity, texture and sensory results were found insignificant (P > 0.05). Total saturated, total unsaturated and trans fatty acid contents of cowpea flour added meatball samples were significantly (P < 0.05) different from the control samples. In the sensory analysis, meatball samples with 4% cowpea flour received the highest general acceptability score. According to the obtained data, cowpea flour can be added up to 4% on basic meat values without changing the textural and sensory properties of Kırklareli meatballs. Cowpea flour could be added as a functional ingredient in meatballs.Graphical

Effects of Feeding Flaxseeds and Rapeseeds on Milk Fatty Acid Composition in Crossbred Dairy Cattle

Effects of feeding oil seeds on fatty acid profile of milk fat was studied on fifteen multiparous HF × Kankrej dairy cows (15 DIM) fed with three experimental diets, viz., T1: control, Total Mixed Ration (TMR) without oil seeds; T2: TMR with ground flaxseeds @ 4.5% and T3: TMR with ground rapeseed @ 4.5%. The experimental TMRs were offered along with 15 kg green cereal fodder as per the guidelines of ICAR standards for 120 days. The milk samples were collected at the end of the experiment for fatty acid analysis. The results revealed significant (p&lt;0.05) increase in the concentration of total unsaturated fatty acids by 21.5 and 29.5% and significant decrease in the saturated fatty acids by 8.0 and 11.0 % in T2 and T3, respectively, as compared to T1. Medium Chain Fatty Acids decreased significantly (p&lt;0.05) in T2 and T3, while Short Chain Fatty Acids remained at par among all treatments. The Long Chain Fatty Acids increased significantly (p&lt;0.05) in T2 and T3 groups, respectively, as compared to T1. The concentration of Omega 3 was found higher by 16.3 and 69.0% in T2 and T3 than T1. The significantly higher Omega 9 fatty acids concentration was found in both T2 and T3 by 32.57 and 30.0%, respectively, as compared to T1. Treatments had no effects on DMI, milk yield and milk composition. It is concluded that feeding flaxseeds and rapeseeds to dairy cows @ 4.5% in TMR altered milk fatty acid composition in beneficial way by increasing healthy fatty acids and decreasing non-desirable fatty acids.

SOLUTION BLOWN OF PLA NANOFIBER CONTAINING OZONATED MORMODICA OIL AND ITS MICROCAPSULES TO OBTAIN ANTIBACTERIAL MEDICAL TEXTILES SURFACES

In the scope of the study, it was aimed to obtain antibacterial nanofiber surfaces containing Momordica oil, its ozonated oil form and its microcapsules forms. First of all, Mormodica oil was exposed to ozone gas for 135 min. After that, crude and ozonated mormodica oil were microencapsulated by using simple coacervation. Subsequently, %10 PLA polymer solution were prepared and used for obtaining PLA nanofiber surface by using solution blowing spinning. Besides, PLA polymer solution were mixed with crude mormodica oil, ozonated mormodica oil and their microcapsules forms and then these solutions were spun by using solution blowing spinning. Obtained ozonated oil, microcapsules and nanofiber surfaces were characterized via measurement of total unsaturated fatty acid amount in the oils, scanning electron microscope, FT-IR analysis and antibacterial activity test. The data showed that mormodica oil were ozonated. Microencapsulation process was done successfully and obtained nanofiber containing mormodica oil and its microcapsules. Moreover antibacterial activity showed that mormodica oil and ozonated mormodica oil showed antibacterial activity against to S.aureus and E.coli bacteria according to the disc diffusion method. The nanofiber surfaces containing ozonated oil and its microcapsules showed antibacterial activity against to S.aureus and E.coli bacteria according to the ASTM E 2149-01 method. As a result, it was obtained biodegradable nanofiber containing microcapsules and showing antibacterial activity.

Heat stress on breeding value prediction for milk yield and composition of a Brazilian Holstein cattle population

Due to the high milk production of Holstein cows, many countries have chosen to import semen to improve local dairy herds. This strategy would be more effective if this semen was used in the same environment conditions in which the bulls were selected. If the effect of genotype by environment (G × E) interaction is not considered, the estimated breeding values (EBVs) may vary, potentially reducing the selection response. We evaluate the impact of heat stress on selection for milk yield and composition of Holstein cows using random regression models. To verify the interference of heat stress in milk yield (MY) and composition traits (fat, protein, total saturated, and total unsaturated fatty acids content in milk), temperature-humidity index (THI) on test-day milk records was used. The threshold value to divide the environments using test-day information from Brazilian Holstein cows was 72 units of THI, i.e., < 72 represented no heat stress and > 72 represented heat stress. Legendre polynomials of second-order (Leg 2) model and two lactation points (33 and 122 DIM) were used to estimate heritabilities and EBVs for five important dairy traits. The heritabilities of milk components and fatty acids were low (0.09-0.29), regardless of lactation period and degree of heat stress, with the exception of protein content (0.30-0.35). Fat content was the only milk component that was reduced according to the degree of heat stress and lactation period. The EBVs tended to decrease in heat stress conditions, thus animals with high genetic potential demonstrated evidence of G × E interaction. However, acclimatization of dairy cows to heat stress in the farm production systems may have been responsible for the low differences among genetic parameters and EBVs with and without heat stress found in this study.

Nutritional Attributes and Phenolic Composition of Flower and Bud of Sophora japonica L. and Robinia pseudoacacia L.

Sophora japonica L. (SJL) and Robinia pseudoacacia L. (RPL) are widely cultivated in China. However, the utilization of their main by-products are limited due to a lack of comprehensive nutritional attributes. Herein, the proximate composition, mineral elements, fatty acids, amino acids, monosaccharides, and phenolics were analyzed to investigate the nutritional attributes of SJL and RPL. Dietary fiber was the main ingredient in SJL and RPL, followed by protein and lipids. The content of Fe in SJL and RPL was highest, especially in flowers of SJL, reaching about 1179.51 mg/kg. The total unsaturated fatty acids accounted for 89.67% of the bud of SJL. Meanwhile, the essential amino acids contents of the flower and bud of SJL and RPL accounted for 35.95-40.59% of total amino acids. The flower of SJL (373.75 mg/g) exhibited the most abundant monosaccharides. Meanwhile, the total phenolics and flavonoid contents in the buds of SJL and RPL were significantly higher than that of the flower, implying the buds possessed better biological activity. Moreover, the bud of SJL possessed the most abundant phenolics. The results provided a reference for the development of functional food derived from SJL and RPL.

Effect of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat

This study was conducted to evaluate the effects of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat. A total of 40, 6-7 weeks New Zealand white × Chinchilla crossbred rabbits with an average weight of 490 ± 4.40 were randomly divided to 4 groups with 5 replications consisting of 2 animals each in a completely randomized design. Rabbits in treatment 1 (T1) was fed basal diet with no Trichilia monadelpha stem bark extract (TMSB), T2, T3 and T4 were fed basal diet with 3mL, 6mL and 9mL TMSB per rabbit/day. Phytochemical analysis of TMSB revealed the presence of tannins (10.95 mg/g), alkaloids (9.22 mg/g), saponins (4.75 mg/g), oxalates (3.10 mg/g), flavonoids (15.88 mg/g), phenols (18.46 mg/g), terpenoids (8.62 mg/g), glycosides (7.11 mg/g) and 2-diphenyl 1-piccrlhydrazyhydrate (430.8 mg/g). Concentrations of vitamins identified in the extract are; vitamin A (2.190 mg/100g), vitamin B1 (0.567 mg/100g), vitamin B2 (0.301 mg/100g), vitamin B3 (0.227 mg/100g), vitamin B9 (0.080 mg/100g), vitamin B12 (0.209 mg/100g) and vitamin C (5.680 mg/100g). Composition of saturated fatty acid (TSFA) decrease with a significant increase in monosaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) (P˂0.05) across the treatments. It was found that TMSB significantly (P˂0.05) influenced the concentration of total unsaturated fatty acid (TSFA). Therefore, it can be concluded that TMSB is capable of modulating the fatty acid and improving the quality of meat from rabbit without causing any deleterious effect on the performance of the animal.

Effects of blended microbial feed additives on performance, meat quality, gut microbiota and metabolism of broilers.

The present study investigated the effects of blend microbial feed additive (BMFA) in diet on performance, meat quality, gut microbiota and metabolism of broilers. In this study 240 seventy-day-old female Wenchang broilers were randomly allocated into four groups with five replicates of 12 broilers each. Broilers in the control group was fed only basal diet (S0), and the other three groups were fed the same basal diet supplemented with 0.2% (S1), 0.4% (S2), or 0.6% (S3) of BMFA, respectively. The trial continued for 54 days. The results showed that broilers in S2 and S3 had lower average daily feed intake (ADFI) compared with S0 and S1 (P < 0.05). However, diet supplementation with BMFA had no significantly influence on the average daily gain (ADG) and the ratio of ADFI to ADG (F/G) (P > 0.05). The highest thigh muscle percentage was observed in S2 (P < 0.05) among all groups. Diet supplementation with BMFA reduced the shear force in both breast and thigh muscles (P < 0.05) of broilers. An increase (P < 0.05) in the total unsaturated fatty acid (USFA), monounsaturated fatty acids (MUFA), and ratio of unsaturated fatty acids to saturated fatty acid (USFA/SFA) in breast muscles was observed in S3 compared with S0. It was found that the S3 had a relatively higher abundance of Lactobacillus (P < 0.001), as well as a lower abundance of the Bacteroides, Rikenellaceae RC9 gut group, Olsenella, Prevotellaceae UCG-001 and Prevotella (P < 0.05) than the S0. Correlation analysis indicated that a total of 17 differential metabolites between the S3 and S0 were significantly correlated with the 7 differential genera microflora. Overall, diet supplementation with 0.6% of BMFA can significantly improve the meat quality of broilers by decreasing the concentration of SFA and enhancing the levels of the total USFA, MUFA and USFA/SFA in breast muscles. Those findings were tightly bound to the higher proportion of Lactobacillus genus in the intestinal tract of broilers influenced by BMFA.

Effect of supplementation of oilseeds co-products on production performance and fatty acids composition of Beetal goats.

This study evaluated the effect of different oilseed co-product supplementations on feed intake, nutrient digestibility, N retention, yields of milk and milk constituents, and milk fatty acid (FA) profile of Beetal goats. In the lactation trial, thirty-six lactating multiparous Beetal goats (45 ± 2.04kg; 15 ± 2.3days in milk) were assigned to four experimental rations according to randomized complete block design. The blocks were balanced for daily milk yield, parity, and body weight. The goats were either fed a maize silage and wheat straw-based basal ration ad libitum (control) or the control ration was supplemented with cotton seed cake, mustard seed cake, or maize oil cake on an iso-N basis. At the end of lactation trial, four goats (44 ± 0.8kg BW; producing 1250 ± 110g milk/day) were selected and moved to individual metabolism crates for a digestibility and N balance experiment, using a Latin square design (4 × 4). Supplementation of the co-products increased intakes of forage mixture (P = 0.002), total dry matter (DM; P < 0.001), neutral detergent fibre (NDF; P = 0.003), and crude protein (CP; P < 0.001). The additional N supplied by the co-products increased (P < 0.001) N retention, yields of milk, fat, protein, and lactose. Further comparison of the rations supplemented with the co-products revealed that the greatest (P < 0.05) increase in yields of milk (240g/day), milk protein (11.6g/day), fats (16.3g/day), and lactose (11.2g/day) was recorded for maize oil cake, as compared to the control. Except C8:0, supplementation of the co-products decreased (P < 0.01) the contents of all de novo-synthesized saturated FAs (SFAs) and increased (P < 0.001) the contents of health beneficial C18:1n-9, C18:1 tans-11, C18:2n-6, C18:3n-3, C20:2n-6, C20:5n3, and total unsaturated FAs in milk fat. Our findings demonstrated that the oilseed co-product inclusion in dairy goat rations significantly improves forage biomass utilization, yields of milk and milk constituents, and milk FAs' profile, with the largest impact being observed for maize oil cake.

Dietary and Animal Strategies to Reduce the Environmental Impact of Pastoral Dairy Systems Result in Altered Nutraceutical Profiles in Milk.

The objective of this study was to evaluate and provide further insights into how dairy cows genetically divergent for milk urea N breeding values [MUNBV, high (2.21 ± 0.21) vs. low (-1.16 ± 0.21); µ ± SEM], consuming either fresh cut Plantain (Plantago lanceolata L., PL) or Ryegrass (Lolium perenne L., RG) herbage, impacted the nutraceutical profile of whole milk by investigating amino and fatty acid composition and applying metabolomic profiling techniques. Both diet and MUNBV, and their interaction term, were found to affect the relative abundance of alanine, glycine, histidine, and phenylalanine in the milk (p &lt; 0.05), but their minor absolute differences (up to ~0.13%) would not be considered biologically relevant. Differences were also detected in the fatty acid profile based on MUNBV and diet (p &lt; 0.05) with low MUNBV cows having a greater content of total unsaturated fatty acids (+16%) compared to high MUNBV cows and cows consuming PL having greater content of polyunsaturated fatty acids (+92%), omega 3 (+101%) and 6 (+113%) compared to RG. Differences in the metabolomic profile of the milk were also detected for both MUNBV and dietary treatments. Low MUNBV cows were found to have greater abundances of choline phosphate, phosphorylethanolamine, N-acetylglucosamine 1-phosphate, and 2-dimethylaminoethanol (p &lt; 0.05). High MUNBV cows had a greater abundance of methionine sulfoxide, malate, 1,5-anhydroglucitol (1,5-AG), glycerate, arabitol/xylitol, 3-hydroxy-3-methylglutarate, 5-hydroxylysine and cystine (p &lt; 0.05). Large differences (p &lt; 0.05) were also detected as a result of diet with PL diets having greater abundances of the phytochemicals 4-acetylcatechol sulfate, 4-methylcatechol sulfate, and p-cresol glucuronide whilst RG diets had greater abundances of 2,6-dihydroxybenzoic acid, 2-acetamidophenol sulfate, and 2-hydroxyhippurate. The results of this study indicate the potential to alter the nutraceutical value of milk from dietary and genetic strategies that have been previously demonstrated to reduce environmental impact.

Effect of Trichilia monadelpha Stem Bark Extract on the Fatty Acid Composition of Rabbit’s Thigh Meat

This study was conducted to evaluate the effects of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat. A total of 40, 6-7 weeks New Zealand white × Chinchilla crossbred rabbits with an average weight of 490 ± 4.40 were randomly divided to 4 groups with 5 replications consisting of 2 animals each in a completely randomized design. Rabbits in treatment 1 (T1) was fed basal diet with no Trichilia monadelpha stem bark extract (TMSB), T2, T3 and T4 were fed basal diet with 3mL, 6mL and 9mL TMSB per rabbit/day. Phytochemical analysis of TMSB revealed the presence of tannins (10.95 mg/g), alkaloids (9.22 mg/g), saponins (4.75 mg/g), oxalates (3.10 mg/g), flavonoids (15.88 mg/g), phenols (18.46 mg/g), terpenoids (8.62 mg/g), glycosides (7.11 mg/g) and 2-diphenyl 1-piccrlhydrazyhydrate (430.8 mg/g). Concentrations of vitamins identified in the extract are; vitamin A (2.190 mg/100g), vitamin B1 (0.567 mg/100g), vitamin B2 (0.301 mg/100g), vitamin B3 (0.227 mg/100g), vitamin B9 (0.080 mg/100g), vitamin B12 (0.209 mg/100g) and vitamin C (5.680 mg/100g). Composition of saturated fatty acid (TSFA) decrease with a significant increase in monosaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) (P˂0.05) across the treatments. It was found that TMSB significantly (P˂0.05) influenced the concentration of total unsaturated fatty acid (TSFA). Therefore, it can be concluded that TMSB is capable of modulating the fatty acid and improving the quality of meat from rabbit without causing any deleterious effect on the performance of the animal.

An endophytic fungus, Piriformospora indica, enhances drought tolerance of trifoliate orange by modulating the antioxidant defense system and composition of fatty acids.

A cultivable endophytic fungus, Piriformospora indica, improves growth and enhances stress tolerance of host plants, but the underlying mechanisms remain unknown. We hypothesized that P. indica enhanced the drought tolerance of the host by regulating the antioxidant defense system and composition of fatty acids. Trifoliate orange (Poncirus trifoliata) seedlings were inoculated with P. indica under ample water and drought stress to analyze the change in plant growth, reactive oxygen species (ROS) levels, antioxidant enzyme activities, non-enzymatic antioxidant concentrations, fatty acid compositions, and expressions of bothantioxidant enzyme genes and fatty acid desaturase (FAD) genes. The 9-week soil water deficit significantly increased the colonization of P. indica to roots, and P. indica promoted the increase of shoot biomass under drought. Soil drought triggered an elevation of hydrogen peroxide in roots, while the inoculated plants had lower levels of ROS (hydrogen peroxide and superoxide anion radicals) and lower degree of membrane lipid peroxidation (based on malondialdehyde levels) under drought. Drought treatment also elevated ascorbic acid and glutathione concentrations, and the elevation was further amplified after P. indica inoculation. Inoculated plants under drought also recorded significantly higher iron-superoxide dismutase (Fe-SOD), manganese-superoxide dismutase (Mn-SOD), peroxidases, catalase, glutathione reductase and ascorbate peroxidase activities, accompanied by up-regulation of PtFe-SOD and PtCu/Zn-SOD expressions. Inoculation with P. indica significantly increased total saturated fatty acids (e.g., C6:0, C15:0, C16:0, C23:0 and C24:0) concentration and reduced total unsaturated fatty acids (e.g., C18:1N9C, C18:2N6, C18:3N3, C18:1N12 and C19:1N9T) concentrations, leading to a decrease in the unsaturation index of fatty acids, which may be associated with the up-regulation of PtFAD2 and PtFAD6 and down-regulation of PtΔ9. It was concluded that the colonization of P. indica can activate enzyme and non-enzyme defense systems and regulate the composition of fatty acids under drought, thus alleviating the oxidative damage to the host caused by drought.

Effect of traditional fat replacement by oleogel made of beeswax and canola oil on processed meat (steak type) quality

Oleogel (OG) has become an alternative fat ingredient for many foods as well as meat and its related meat products due to its health beneficial effects. The objective of this study was to characterize the processed meat properties as well as analyse the trends of consumer acceptability of meat products by using OG utilization as a fat replacer. Processed meat samples were prepared for 3 groups including i) processed meat (only red part) without any additive addition: control, ii) 50% beef tallow adding: BT, and iii) 50% OG, obtained from beeswax and canola oil mixture, substituting of BT: OG. All meat samples determined the physical, physicochemical, and lipid profiles as well as sensorial properties. The results found that OG treatment showed desirable physical and physicochemical characteristics. The soft texture of the meat sample was observed in the OG group when compared with other treatments. Furthermore, OG replacing the traditional fat in processed meat has significantly reduced the percentage of total saturated fatty acid (SFA, 68.37±2.19) and increased the total unsaturated fatty acid (USFA, 31.63±1.63) (p &lt; 0.05) when compared with BT group treatment (SFA: 79.52±3.72 and USFA: 20.48±0.31). OG treatment also showed improvement in processed meat through the significantly higher score of overall liking from the consumer evaluation (p &lt; 0.05). The results of this study could be used as preliminary results for the development of healthy meat model products, contributing to driving meat consumption together with the reduction of risk factors of metabolic diseases.

Oxidative stability of high oleic palm and hazelnut skin oil blends

AbstractHigh oleic palm oil (HOPO) with high oleic and palmitic acid contents is a new vegetable oil that needs to find further use in food applications, while hazelnut skin oil (HSO) with high oleic acid content is a by‐product of hazelnut oil production and have no commercial value. The aim of the study was to obtain new oil blends for new food formulations with high oxidative stability and low (or reasonable) saturated fatty acid content using HOPO and HSO. For this aim, they were blended in the proportions of 0 to 100% (wt/wt), and fatty acid and tocol compositions, induction period and storage stability of the blends were evaluated. Tocotrienols (560.94 mg/kg) constitute the majority of the tocol content of HOPO, while tocopherols (437.69 mg/kg) were the major tocols of HSO. HOPO has a higher saturated fatty acid composition and lower monounsaturated fatty acids content than HSO. In general, it was determined that increasing the HSO ratio in oil blends reduced their oxidative stabilities, which is most likely because HSO is richer in total unsaturated fatty acids (91.8%) than HOPO (68.4%). In addition, tocotrienols are known to be stronger antioxidants than tocopherols. Although HOPO is rich in γ‐tocotrienol, which has a strong antioxidant activity, it also has some disadvantages such as having a high price and containing significant levels of saturated fatty acids (mainly palmitic acid). The optimum blend ratio was then determined as 50% HOPO:50% HSO considering oxidative stability and fatty acid compositions.

Nitrogen Starvation Enhances the Production of Saturated and Unsaturated Fatty Acids in Aurantiochytrium sp. PKU#SW8 by Regulating Key Biosynthetic Genes.

Nitrogen deprivation is known to improve lipid accumulation in microalgae and thraustochytrids. However, the patterns of fatty acid production and the molecular mechanisms underlying the accumulation of unsaturated and saturated fatty acids (SFAs) under nitrogen starvation remain largely unknown for thraustochytrids. In this study, batch culture experiments under nitrogen replete and nitrogen starvation conditions were performed, and the changes in the transcriptome of Aurantiochytrium sp. PKU#SW8 strain between these conditions were investigated. Our results showed improved yields of total fatty acids (TFAs), total unsaturated fatty acids, and total SFAs under nitrogen starvation, which suggested that nitrogen starvation favors the accumulation of both unsaturated and saturated fatty acids in PKU#SW8. However, nitrogen starvation resulted in a more than 2.36-fold increase of SFAs whereas a 1.7-fold increase of unsaturated fatty acids was observed, indicating a disproportionate increase in these groups of fatty acids. The fabD and enoyl-CoA hydratase genes were significantly upregulated under nitrogen starvation, supporting the observed increase in the yield of TFAs from 2.63 ± 0.22 g/L to 3.64 ± 0.16 g/L. Furthermore, the pfaB gene involved in the polyketide synthase (PKS) pathway was significantly upregulated under nitrogen starvation. This suggested that the increased expression of the pfaB gene under nitrogen starvation may be one of the explanations for the increased yield of docosahexaenoic acid by 1.58-fold. Overall, our study advances the current understanding of the molecular mechanisms that underlie the response of thraustochytrids to nitrogen deprivation and their fatty acid biosynthesis.

Fatty Acids of Erythrocyte Membranes and Blood Serum in Differential Diagnosis of Inflammatory Bowel Diseases

Aim: to study fatty acid levels in erythrocyte membranes (RBC) and blood serum (BS) in patients with inflammatory bowel diseases (IBDs) to develop differential diagnostic models including fatty acids as biomarkers to distinguish between nosological entities of IBDs (ulcerative colitis — UC, Crohn's disease — CD, unclassified colitis — UCC).Materials and methods. We examined 110 patients (mean age 37,7 ± 12,1 years) with IBDs and 53 healthy patients in control group (43,3 ± 11,7 years). The IBDs group included 50 patients with UC, 41 patients with CD, 19 patients with UCC. An exacerbation of the disease was revealed in 42 patients (84 %) with UC, 34 patients with CD (82.9 %) and 11 people with UCC (57.9 %). The study of fatty acids (FA) composition of RBC membranes and BS was carried out using GC/MS system based on three Agilent 7000B quadrupoles (USA).Results. The most significant for distinguishing active UC from CD exacerbation were serum levels of elaidin (p = 0.0006); docosatetraenoic (n-6) (p = 0.004); docodienic (n-6) (p = 0.009); omega-3/omega-6 ratio (p = 0.02); docosapentaenoic (n-3) (p = 0.03); the sum of eicosapentaenoic and docosahexaenoic (p = 0.03), as well as the content of RBC lauric FA (p = 0.04) (AUC — 0.89, sensitivity — 0.91, specificity — 0.89, diagnostic accuracy — 0.91). To distinguish active UC from the same of UCC, the following serum FA were found to be significant: alpha-linolenic; saturated (pentadecanoic, palmitic, stearic, arachidic); monounsaturated (palmitoleic, oleic); omega-6 (hexadecadienic, arachidonic) (p = 0.00000011—0.03300000) (AUC — 0.995, sensitivity — 0.98, specificity — 0.96, diagnostic accuracy — 0.97). The most significant in distinguishing patients with active CD from UCC exacerbation were levels of the following FA: alpha-linolenic; palmitoleic; oleic; the amount of saturated fatty acids (SFA); total unsaturated fatty acids (UFA); stearic; monounsaturated fatty acids (MUFA) amount; SFA/UFA; SFA/PUFA (polyunsaturated fatty acids); linoleic; total PUFA n6; lauric; arachidic acid (p = 0.0000000017–0.030000000) (AUC — 0.914, sensitivity — 0.90, specificity — 0.87, diagnostic accuracy — 0.91).Conclusion. The study of FA levels in groups with different nosological forms of IBDs using complex statistical analysis, including machine learning methods, made it possible to create diagnostic models that differentiate CD, UC and UCC in the acute stage with high accuracy. The proposed approach is promising for the purposes of differential diagnosis of nosological forms of IBDs.