Total Transmittance Research Articles

Triple-broadband asymmetric transmission via cross-polarization conversion based on composite chiral metasurface structure

In this paper, a novel composite chiral metasurface (CCMS) was proposed and investigated, which is composed of a rectangle-patch-ring resonator (RPRR) structure sandwiched between two twisted sub-wavelength metal gratings separated by a dielectric substrate. Both simulation and experimental results demonstrate that the proposed CCMS achieves a significant asymmetric transmission (AT) effect and efficient cross-polarization conversion for the incident linear polarization wave in a triple-broadband range. Simulation results are in good agreement with the experiments. Specifically, the CCMS converts normal impinging y-/x-polarization wave along the forward/backward (-z/+z) direction into transmitted x-/y-polarization wave with the cross-polarization transmission coefficient over 0.7 at 4.15–5.5 GHz, 7.29–10.77 GHz and 12.49–16.59 GHz, respectively. In addition, the AT coefficient (Δlin) and the total transmittance (Tx) of the x-polarization wave propagation along the –z axis direction are both over 0.5 in the triple-broadband frequency range. The simulated polarization azimuth rotation and ellipticity angles, induced surface current, and electric field vector distributions further confirm the characteristics of the triple-broadband high-efficiency AT effect and cross-polarization conversion of the designed CCMS. The proposed CCMS design serves as an important reference for practical applications of microwave devices.

Antimicrobial and optical properties of a new biogenic silica-coated silver nanoparticles incorporated into experimental resin.

Evaluate the effects of incorporating silica-coated silver nanoparticles (Ag@SiO2 NPs) into odontological clinic resin materials. Silver nanoparticles coated with silicon dioxide were added to the experimental resin matrix at 1, 3, and 5wt%. Degree of conversion (DC), optical properties (total transmittance and color change), and microstructural analysis were evaluated. Materials were tested for silver ion release, cytotoxicity in dental pulp fibroblasts, Streptococcus mutans biofilm growth by Colony-Forming Unit (CFU) and confocal laser scanning microscopy (CLSM). Groups had a similar DC, despite significant differences observed in transmittance and color change analysis for all groups with NPs. Silver ion release values were below the detection limit after 72h for all groups, and NPs incorporation did not show a statistical difference from the control on pulp fibroblasts assay. After 72h, the CFU count was significantly reduced by 74% from 3wt% of Ag@SiO2NPs. CLSM evaluation on S. mutans colonies showed a dose-dependent decrease in the emitted fluorescence. The application of Ag@SiO2 NPs in a resinous matrix, demonstrates a significant reduction of S. mutans CFU in oral biofilm, at concentrations from 3wt%, without an increase in cytotoxicity. The reduced transmittance values did not affect the DC, although a significant color change was perceived in all concentrations. Key words:Nanoparticles, Silver Compounds, Composite Dental Resin, Anti-Bacterial Agent, Optical Imaging.

Passive Building Energy Saving: Building Envelope Retrofitting Measures to Reduce Cooling Requirements for a Residential Building in an Arid Climate

In arid climates, a significant portion of the urban peak energy demand is dedicated to cooling and air-conditioning during the summer. The rapid urbanization rates in developing countries, particularly in the Gulf Cooperation Council (GCC), have intensified the pressure on energy resources to meet the indoor comfort needs of residents. As a result, there has been a substantial increase in energy demand, with a 2.3% rise recorded in 2018. Electricity consumption in residential buildings accounted for over 48.6% of the total electricity consumption. The choice of building fabrics used in a residential building can significantly impact the building’s passive performance and carbon footprint. This study aimed to enhance our understanding of how specific fabric details influence cooling energy usage in arid climates. To achieve this, a validation simulation model was initially created as a base case for a residential housing typology in Al Ain, UAE. This was followed by a parametric energy evaluation of various building envelope features. The evaluation was based on the reduction of yearly cooling load energy. The simulation results indicate that incorporating 50 mm of expanded polystyrene insulation into the outside walls significantly reduced energy consumption for cooling requirements in the arid UAE climate. Furthermore, no substantial difference was observed in the various roofing choices, including cool and green roofs, gravels, and sand roofs. Additionally, we concluded that the total solar energy transmittance (g-value) of windows played a more significant role than thermal transmittance (U-value) in reducing solar heat gain within the spaces. These findings should guide strategic decisions on building envelope upgrading for sustainable societies.

Comprehensive investigation of a building integrated crossed compound parabolic concentrator photovoltaic window system: Thermal, optical and electrical performance

Integrating PV solar cells with concentrators into window systems can not only generate electricity for a building, but also has the potential to enhance the thermal resistance of the building's windows without a significant sacrifice of light transmittance for passive daylight. A novel system, known as the crossed compound parabolic concentrator photovoltaic window, has been recently studied for its electrical properties. However, its thermal and optical performance, particularly in terms of the overall heat transfer coefficient (U-value) and total optical transmittance when integrated into a building, remains unexamined. These factors are crucial for predicting the system's impact on a building's energy efficiency and indoor comfort. Therefore, this paper aims to investigate the U-value of this window system under various temperature scenarios and the optical transmittance of the window at different incident angles. The thermal conductance was assessed through numerical simulations using a computational fluid dynamics model, which was validated by experimental measurements conducted in a large climate chamber. The optical transmittance was investigated using a validated 3D ray-tracing model. The total optical transmittance and electricity generation were calculated for typical sunny days in winter and summer under London's climate conditions. Additionally, alternative designs were developed to explore the impact of pitch between adjacent optics on the thermal conductance and optical transmittance of the window. The results showed that the window with a configuration of Dx = Dy = 5 mm (where Dx and Dy represent the horizontal and vertical pitches, respectively, between two adjacent solar optics) achieved the lowest U-value (2.566 W/m2·K). This U-value is slightly lower than that of the original window design, which has a U-value of 2.575 W/m2·K. The original window configuration with Dx = Dy = 1.77 mm produces the highest power output. Specifically, it generates 499.25 Wh/m2 on a typical sunny day in winter and 162.73 Wh/m2 on a typical sunny day in summer. However, it exhibits the lowest transmittance (14.6 % on a typical sunny day in winter and 25.2 % on a typical sunny day in summer, respectively), indicating that it is more suitable for buildings with a higher window-to-wall ratio to ensure an adequate amount of natural light. For buildings with a lower window-to-wall ratio, the CCPC-PV window should be designed with a larger horizontal pitch, such as 15 mm and 30 mm, to meet indoor illuminance requirements while also providing enhanced thermal performance and additional power output.

Comparison of yield and properties of banana juice extracted by hot water extraction method and different enzymes and preparation of wine from the extracted juices

AbstractThe yield and properties of banana juice extracted by enzymatic pretreatments using different enzymes and were compared among themselves and with hot water extraction. The treatments that gave higher yield and better properties of the juices were used for winemaking and the properties of the wines were also compared. A comparison of gas chromatography‐mass spectrometry analysis was also done between the juices and the wines. The various treatments were hot water bath extraction and different enzymes (amylase, cellulase, pectinase, and viscozyme) at two concentrations (0.05% and 0.1%). Juice yield, total soluble sugars, pH, titrable acidity, reducing sugars, and transmittance were analyzed. Best results for juice recovery (46.44 ± 0.50%), total soluble solids (14.8 ± 0.01 °Brix), and transmittance (86.93 ± 2.68%) were achieved using pectinase (0.1%), while viscozyme (0.1%) yielded best results for viscosity (1.49 ± 0.09 mPa.s), pH (5.01 ± 0.05), and acidity (0.277 ± 0.01%). The banana juices from these treatments were then used for wine preparation, and the physicochemical parameters were monitored during a 30‐day storage period. Within 20 days during fermentation, a reduction in total soluble solids from 22 °Brix to 6.1 °Brix was observed. Juice from banana pulp can be successfully extracted using the enzymes pectinase and viscozyme, and wine can be prepared from the enzymatically extracted juices.

Thermal performance of indoor solar shading devices using a thermal nodal model and a lighting simulation model

Various facade designs, such as light shelves, have been proposed to achieve zero emission buildings. However, general thermal load simulation models are unable to properly evaluate the thermal effects of spaces near windows, such as light shelves and counters, which are installed on the interior side of windows for increased daylight utilization. In this study, we propose a method to evaluate the thermal performance of indoor light shelves. The model proposed herein consists of three components: A thermal nodal model of the window area, a lighting simulation model, and a thermal simulation model. The thermal nodal model places a node at each device (glass, ceiling, light shelf, and floor) in the light shelf space. The heat balance equation for each node is then solved. In doing so, the total transmittance of the entire indoor-installed device space, which considers inter-reflections and the solar radiation absorptance of each device, is calculated using the Radiance software. Additionally, the solar heat gain obtained using the thermal nodal model is substituted internally into NewHASP, and the thermal load calculation is performed. Finally, a simulation is performed using an office model, and the indoor-installed light shelf exhibited a 14 % reduction in annual thermal load.

Fabrication of antireflective coatings with self-cleaning function using Si–Ti modified hollow silicon mixed sol

The antireflective coating (ARC) is fabricated by the sol-gel method using mixed sol modified by Si–Ti composite sol. The effects of the mixing ratio of Si–Ti composite sol and hollow silica sol on the surface morphology, optical properties, mechanical properties, and wetting ability of the ARC were studied. Moreover, the self-cleaning ability and environmental stability were examined via dip coating the modified sol on glass substrates. The proposed ARC exhibited a total solar-weighted transmittance (Тsw) of more than 94.97% over a wavelength range of 380–1100 nm, significantly higher than that of the bare glass substrate (Тsw = 90.62%). After modification, the proposed ARC exhibited a hardness of 3 H. In addition, the coating presented an extremely hydrophilic surface with a minimum water contact angle of less than 5°. Water droplets resulted in the formation of a water film on the ARC surface, which could significantly reduce the adverse effects of subsequent pollutants on the coating transmittance; simultaneously, owing to the introduction of TiO2, the coating could oxidatively decompose organic contamination. Finally, damp test results showed that the ARC transmittance only decreased by 0.05%, indicating good environmental stability.

Prediction of soluble solids content using near-infrared spectra and optical properties of intact apple and pulp applying PLSR and CNN

Soluble solids content (SSC) is one of the most important internal quality attributes of fruit and could be predicted using near-infrared (NIR) spectra and optical properties. Partial least squares regression (PLSR) is a conventional regression method in SSC prediction. In recent years, deep learning methods represented by convolutional neural network (CNN) was suggested to be implied in spectral analysis. However, researchers are inevitably facing problems with regard to the selection of spectral pretreatment methods and the evaluation of the performance of the chosen regression. This study employed PLSR and CNN regression to predict SSC of apple based on the collected diffuse reflectance spectra of intact apple, total reflectance and total transmittance spectra of apple pulp, and the calculated optical property spectra, i.e., absorption coefficient and reduced scattering coefficient spectra of apple pulp. Five different spectral pretreatment methods were exerted on these spectra. Results showed that at a given regression (PLSR or CNN), the built models based on the diffuse reflectance spectra of intact apple had the best SSC prediction, and the built models based on pulp’s reduced scattering coefficient spectra had the poorest prediction performance. The best prediction performance was achieved by PLSR models using Savitzky-Golay with multiple scattering correction (Rp = 0.96, RMSEP = 0.54 %) and CNN regressions using Savitzky-Golay with standard normal variational transformation (Rp = 0.95, RMSEP = 0.59 %), respectively. Additionally, when the unknown original spectra were used for modeling, CNN had a better performance compared to PLSR, indicating the outstanding preponderance of CNN in spectral analysis. This study provides an effective reference for the selection of chemometric method based on NIR spectra.

Uncertainty of atmospheric scattering functions relevant for Satellite Ocean Colour Radiometry in European Seas

ABSTRACT Uncertainty assessment of Ocean Colour Radiometry (OCR) usually relies on the comparison between satellite marine reflectance and concurrent in situ radiometric measurements. However, when dealing with the standard atmospheric correction (AC), uncertainty of marine reflectance originates fundamentally from two sources: (i) uncertainty of the Level-1 calibration at top of the atmosphere, and (ii) uncertainty of the atmospheric scattering functions computed by the AC (atmospheric path reflectance and transmittance). The aim of this alternative work is thus to assess the uncertainty of these scattering functions, thanks to inherent optical properties of aerosols extracted from the AErosol Robotic NETwork (AERONET) and ingested in a radiative transfer code. The study is conducted for the MEdium Resolution Imaging Spectrometer (MERIS), over three AERONET sites selected in European seas (Aqua Alta Oceanographic Tower, AAOT; Gustav Dalen Tower, GDT; Lampedusa) and Lanai in the Pacific Ocean as a reference. We develop an uncertainty budget based on the statistical analysis of the errors between MERIS and AERONET-derived data. At 443 nm, uncertainty of MERIS atmospheric path reflectance due to random effects is estimated around 2%, while that of total atmospheric transmittance is between 0.6% and 1%. Propagation of these uncertainties to OCR is achieved following metrological principles and varies between match-ups and sites due to the distinctive contribution of the marine signal to the total radiometry: uncertainty of MERIS marine reflectance at 443 nm is 10.8% (±1.1%) at Lanai, 18.3% (±4.6%) at Lampedusa, 29.4% (±12.2%) at AAOT and up to 68.7% (±127.4%) at GDT (i.e. with a significant intra-site variability). The methodology is a first step aimed at deriving a pixel-by-pixel uncertainty budget of OCR, instead of providing overall estimates as generally done in the OC community. Limitations due to uncertainties in the AERONET data are also discussed, with a tentative quantification.

Enhancing Light Extraction Efficiency in OLED Using Scattering Structure-Embedded DMD-Based Transparent Composite Electrodes.

This study investigates the application of scattering structures to the metal layer in a DMD (Dielectric/Metal/Dielectric) configuration through plasma treatment. The purpose is to enhance the light extraction efficiency of organic light-emitting diodes (OLEDs). Different plasma conditions were explored to create scattering structures on the metal layer. The fabricated devices were characterized for their electrical and optical properties. The results demonstrate that the introduction of scattering structures through plasma treatment effectively improves the light extraction efficiency of OLEDs. Specifically, using O2-plasma treatment on the metal layer resulted in significant enhancements in the total transmittance, haze, and figure of merit. These findings suggest that incorporating scattering structures within the DMD configuration can effectively promote light extraction in OLEDs, leading to enhanced overall performance and light efficiency.

The Impact of Climate Change and Window Parameters on Energy Demand and CO2 Emissions in a Building with Various Heat Sources

This article presents an original study on the impact of climate change and the area of windows Awi (factor X1), the thermal transmittance coefficient of windows Uwi (factor X2), and the coefficient of total solar transmittance factor of the glazing ggl (factor X3) on the index of annual usable energy demand for heating EUH (function Y) of a single-family residential building in the climatic conditions of Bialystok (Poland), which were loaded with an equal gradual increase in average monthly external temperature by Δθe,n (factor X4). Based on the results of the computational experiment, a deterministic mathematical model of this dependence was developed, and the effects of selected factors on the Y function were analyzed for the considered climatic conditions. Moreover, in cases of selected variants, the influence of the energy source on the amount of final energy used and CO2 emissions was studied. It was found that an increase in the average monthly external temperature reduces the EUH of the tested building by 8.4% per every 1 °C of increase in Δθe,n. The reduction in CO2 emissions as a result of climate change is visible for systems with low efficiency and high emission factors (wood boiler), while in the case of pro-ecological high-efficiency systems (with a ground-source pump heat) it is inappreciable. Due to the need to decarbonize buildings, knowledge about the impact of the properties of windows, which are the weakest element in terms of heat loss through the building envelope, as well as the type of heat source on heat demand and CO2 emissions, is very important for engineers and designers when making the correct decisions.

Fabrication of tungsten-based optical diffuser using fiberform nanostructure via efficient plasma route.

Optical diffusion is an essential process used to manage photons in a wide range of photoelectric systems. This work proposes an approach to fabricate novel optical diffusers by a plasma-processing technique, using fiberform nanostructures formed by helium plasma irradiation and subsequent annealing. After an annealing procedure in the air for oxidation, the optical properties and the light-diffusing abilities of these nanostructured thin films were studied. In addition to the morphology analysis and total transmittance measurement, the diffusion efficiency of the optical diffusers was analyzed using a transmitted scatter distribution function (TDF). It was revealed that the diffusion efficiency of a device with an irradiation time of 30 minutes could reach 97%. The results demonstrate the potential of these nanostructured optical diffusers for various photoelectric applications.

Weak Localization of Light in a Magneto-Active Medium

The interference contribution to the optical conductance (total transmittance) of a sample of a disordered Faraday medium is calculated. The suppression of wave interference in a magnetic field is shown to be due to helicity-flip scattering events. The magnetic field does not destroy the interference of waves with a given helicity, but suppresses it if the helicity changes along different parts of the wave trajectory. This leads to a decrease in the interference contribution to the conductance with increasing the magnetic field. A similar phenomenon, negative magnetoresistance, is known as a consequence of weak localization of electrons in metals with impurities. It is found that, as the magnetic field increases, the change in the interference correction to the optical conductance tends to a certain limiting value, which depends on the ratio of the transport mean free path to the helicity-flip scattering mean free path. We also discuss the possibility of controlling the transition to the regime of strong “Anderson” localization in the quasi-one-dimensional case by means of the field.

Photonic crystal broadband y-shaped 1 × 2 beam splitter inversely designed by genetic algorithm

A novel two-dimensional photonic crystal broadband Y-shaped 1 × 2 beam splitter is proposed. Broadband performance of the 1 × 2 beam splitter can be greatly improved by optimizing the z-axis offset of the dielectric rods adjacent to the input and output waveguides, and the offset along the V-shaped oblique waveguide of the dielectric rods at the junction area. To enhance the optimization efficiency and achieve excellent splitting performance of the 1 × 2 beam splitter, the genetic algorithm is applied to inversely design the above offsets of the 1 × 2 beam splitter. The results show that, in the bandwidth range of 1515 to 1587 nm, the highest and lowest transmittance of the 1 × 2 beam splitter are 99.6% and 97.9%, respectively, the additional loss is <0.1 dB, and the uniformity tends to 0 dB. Finally, the tolerance analysis of the 1 × 2 beam splitter is carried out. When the deviation of each variable reaches ±10 nm, the total transmittance of the 1 × 2 beam splitter in the bandwidth range of 1515 to 1587 nm is still higher than 95.2%, with the additional loss <0.22 dB. Due to the virtues of broad operating bandwidth, high transmittance, good uniformity, and strong robustness, the proposed 1 × 2 beam splitter will have great application prospects in the field of photonic integrated circuits, all-optical communication network, and so on.

Optoelectronic performance of indium tin oxide thin films structured by sub-picosecond direct laser interference patterning

A route to increase the efficiency of thin film solar cells is improving the light-trapping capacity by texturing the top Transparent Conductive Oxide (TCO) so that the sunlight reaching the solar absorber scatters into multiple directions. In this study, Indium Tin Oxide (ITO) thin films are treated by infrared sub-picosecond Direct Laser Interference Patterning (DLIP) to modify the surface topography. Surface analysis by scanning electron microscopy and confocal microscopy reveals the presence of periodic microchannels with a spatial period of 5 µm and an average height between 15 and 450 nm decorated with Laser-Induced Periodic Surface Structures (LIPSS) in the direction parallel to the microchannels. A relative increase in the average total and diffuse optical transmittances up to 10.7% and 1900%, respectively, was obtained in the 400–1000 nm spectral range as an outcome of the interaction of white light with the generated micro- and nanostructures. The estimation of Haacke’s figure of merit suggests that the surface modification of ITO with fluence levels near the ablation threshold might enhance the performance of solar cells that employ ITO as a front electrode.

Artificial neural network-based determination of denoised optical properties in double integrating spheres measurement

Accurate determination of the optical properties of biological tissues enables quantitative understanding of light propagation in these tissues for optical diagnosis and treatment applications. The absorption ([Formula: see text]) and scattering ([Formula: see text]) coefficients of biological tissues are inversely analyzed from their diffuse reflectance (R) and total transmittance (T), which are measured using a double integrating spheres (DIS) system. The inversion algorithms, for example, inverse adding doubling method and inverse Monte Carlo method, are sensitive to noise signals during the DIS measurements, resulting in reduced accuracy during determination. In this study, we propose an artificial neural network (ANN) to estimate [Formula: see text] and [Formula: see text] at a target wavelength from the R and T spectra measured via the DIS to reduce noise in the optical properties. Approximate models of the optical properties and Monte Carlo calculations that simulated the DIS measurements were used to generate spectral datasets comprising [Formula: see text], [Formula: see text], R and T. Measurement noise signals were added to R and T, and the ANN model was then trained using the noise-added datasets. Numerical results showed that the trained ANN model reduced the effects of noise in [Formula: see text] and [Formula: see text] estimation. Experimental verification indicated noise-reduced estimation from the R and T values measured by the DIS with a small number of scans on average, resulting in measurement time reduction. The results demonstrated the noise robustness of the proposed ANN-based method for optical properties determination and will contribute to shorter DIS measurement times, thus reducing changes in the optical properties due to desiccation of the samples.

66‐2: Highly Transparent, Colorless Optical Film with Outstanding Mechanical Strength and Folding Reliability Using Mismatched Charge‐Transfer Complex Intensification

This study describes a novel colorless polyimide window film using the concept of charge‐transfer complex (CTC) intensification. The resulting film has a tensile modulus of 8.4 GPa, total transmittance of ≈90%, and yellow index below 3. This is the best‐recorded balance between mechanical strength and optical properties for a highly flexible optical film. Additionally, the film exhibits a pencil hardness grade over 2H and folding reliability over 200,000 folding/unfolding cycles. These outstanding properties are attributed to the unique supramolecular structure, which features multiple hydrogen bonding and salt complexation interactions that lead to CTC intensification.

Predictable esthetics in hybrid and resin-based CAD/CAM restorative materials: Translucency as a function of material thickness.

The CAM of esthetically pleasing monolithic dental restorations presents with specific challenges. One vital parameter to consider is the translucency of the materials. Previous studies have proven a correlation between translucency and material thickness for various all-ceramic materials. The aim of the present study was to assess and define the relationship between thickness and translucency in modern resin-based restorative materials. Specimens fabricated from two resin nano-ceramics (Cerasmart, Lava Ultimate), a polymer-infiltrated ceramic network (Vita Enamic), and a polymethyl methacrylate (Telio CAD) were examined, representing these different material classes. For each material, 12 specimens (n = 12) were fabricated in five thicknesses (0.4, 0.7, 1.0, 1.3, and 1.6 mm; N = 240). The translucency was measured with a spectrophotometer. The total light transmittance for each specimen was calculated applying specialized software. Regression curves were fitted to the results and their coefficient of determination (R2) fit was determined. Logarithmic regression curves showed the best R2 approximation (Cerasmart: R2 = 0.994; Vita Enamic: R2 = 0.978; Lava Ultimate: R2 = 0.997; Telio CAD: R2 = 0.997) to the light transmission values. The results of the present study indicate that the translucency of resin-based materials can be calculated using a mathematic approach to estimate their optical behavior. Cerasmart, Lava Ultimate, Vita Enamic, and Telio CAD exhibit a logarithmic relationship between material thickness and translucency. By determining material-specific coefficients for this logarithmic function, the resulting translucency can be computed for any given material thickness.

High Refractive Index Monomers for Improving the Holographic Recording Performance of Two-Stage Photopolymers.

Photopolymers hold great promise for the preparation of transparent volume holographic gratings (VHG), which are core optical elements in many application fields. To improve the holographic recording property of a two-stage photopolymer, four new (meth)acrylate monomers (CTA, CTMA, CTBA, CTBMA) with high refractive indices (1.59-1.63) are designed and synthesized in this study. Using them as one writing monomer, a series of photopolymer samples with different formulations and thicknesses are fabricated for holographic recording. Among them, a formulation containing 9 wt % CTMA shows the best performance. Using it as a recording medium, a VHG with high resolution and diffraction efficiency is constructed. Its refractive index modulation reaches 0.046. Moreover, its total transmittance within 400-800 nm achieves 96.62% after photobleaching. The results indicate that the CTMA-based formulation has great application potential in developing high-performance transparent VHG.

Scattering and absorption properties modification of optically cleared skeletal muscles: an ex vivo study.

Optical clearing is a relatively new approach to enhancing the optical transparency of biological tissues by reducing their scattering properties. The optical clearing effect is achievable via various chemical, physical, and photo-thermal techniques. The present work studied optical parameters of bovine skeletal muscles under different clearing protocols: immersion optical clearing in 99% glycerol and photo-thermal optical clearing via exposure to IR laser irradiation. Moreover, the two techniques were combined with different immersion time intervals after multiple exposure periods to get optimum results. The muscle samples' diffuse reflectance and total transmittance were measured using a single integrating sphere and introduced to the Kubleka-Munk mathematical model to determine the absorption and reduced scattering coefficients. Results revealed a 6% scattering reduction after irradiating the sample for 10min and immersing it in glycerol for 18min and 8% after 20min of laser irradiation and 18min of immersion. Moreover, increases of 6.5% and 7.5% in penetration depth were prominent for the total treatment times of 28min and 38min, respectively. Furthermore, the measurements' accuracy and sensitivity were analyzed and evaluated using the receiver operating characteristic method. The accuracy ranged from 0.93 to 0.98, with sensitivity from 0.93 to 0.99 for each clearing protocol. Although laser irradiation and application of 99% glycerol separately produced scattering light reduction, the maximal clearing effect was obtained while irradiating the sample with a laser for 20min and then immersing it in 99% glycerol for a maximum of 18min.